Restriction map for
pFN21AB7038
(Circular) (Six-base) MAPSORT of: pFN21AB7038.seq Check: 1824 from: 1 to: 6076
pFN21AB7038 6076 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3057 3057
Size: 6076
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3886 278
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3419 1937
Size: 1482 4594
Acc65I G'GTAC_C
Cuts at: 3397 3397
Size: 6076
AclI AA'CG_TT
Cuts at: 1563 4203 4576 1563
Size: 2640 373 3063
Fragments arranged by size:
3063 2640 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 2813 2990 3116 4139 5523 5791 1632
Size: 1046 135 177 126 1023 1384 268 1917
Fragments arranged by size:
1917 1384 1046 1023 268 177 135 126
AfeI AGC'GCT
Cuts at: 2609 2765 2609
Size: 156 5920
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5879
AflIII A'CryG_T
Cuts at: 1203 2127 2196 2727 4975 5952 1203
Size: 924 69 531 2248 977 1327
Fragments arranged by size:
2248 1327 977 924 531 69
AgeI A'CCGG_T
Cuts at: 4907 4907
Size: 6076
AhdI GACnn_n'nnGTC
Cuts at: 4805 4805
Size: 6076
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6076
AlwNI CAG_nnn'CTG
Cuts at: 3819 5391 3819
Size: 1572 4504
ApaI G_GGCC'C
Cuts at: 3059 3281 3059
Size: 222 5854
ApaLI G'TGCA_C
Cuts at: 4133 5289 4133
Size: 1156 4920
ApoI r'AATT_y
Cuts at: 1493 3385 3611 3763 5872 1493
Size: 1892 226 152 2109 1697
Fragments arranged by size:
2109 1892 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4630 160
Size: 872 3598 1606
Fragments arranged by size:
3598 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2167 2342 2719 2864 2920 3020
Size: 73 126 98 175 377 145 56 100
Cuts at: 3020 3070 3401 1870
Size: 50 331 4545
Fragments arranged by size:
4545 377 331 175 145 126 100 98
73 56 50
BamHI G'GATC_C
Cuts at: 3406 3773 3406
Size: 367 5709
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2258 2662 2807 2903 3010
Size: 325 217 627 471 404 145 96 107
Cuts at: 3010 3397 4852 618
Size: 387 1455 1842
Fragments arranged by size:
1842 1455 627 471 404 387 325 217
145 107 96
BanII G_rGCy'C
Cuts at: 1391 2070 2189 2228 2339 2408 2531 2696 2845
Size: 679 119 39 111 69 123 165 149
Cuts at: 2845 2999 3059 3281 3395 1391
Size: 154 60 222 114 4072
Fragments arranged by size:
4072 679 222 165 154 149 123 119
114 111 69 60 39
BbeI G_GCGC'C
Cuts at: 2262 2811 3014 2262
Size: 549 203 5324
Fragments arranged by size:
5324 549 203
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2713 2747 4267 4301 1903
Size: 34 50 34 692 34 1520 34 3678
Fragments arranged by size:
3678 1520 692 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3968 5178 3968
Size: 1210 4866
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6076
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2291 2300 2855
Size: 107 122 71 631 605 619 9 555
Cuts at: 2855 2882 4687 136
Size: 27 1805 1525
Fragments arranged by size:
1805 1525 631 619 605 555 122 107
71 27 9
BglII A'GATC_T
Cuts at: 2556 6071 2556
Size: 3515 2561
BlpI GC'TnA_GC
Cuts at: 2549 2993 3493 2549
Size: 444 500 5132
Fragments arranged by size:
5132 500 444
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2667 2906 3059 3281 4137 5293 1790
Size: 244 633 239 153 222 856 1156 2573
Fragments arranged by size:
2573 1156 856 633 244 239 222 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6076
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3807 4765 442
Size: 416 53 83 399 24 2390 958 1753
Fragments arranged by size:
2390 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6076
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 6076
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 4736 1239
Size: 829 2668 2579
Fragments arranged by size:
2668 2579 829
Bpu10I CC'TnA_GC
Cuts at: 2333 2429 2333
Size: 96 5980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2341 3194 4201 5066 5364 2341
Size: 853 1007 865 298 3053
Fragments arranged by size:
3053 1007 865 853 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4739 882
Size: 617 3240 2219
Fragments arranged by size:
3240 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2130 2209 3867 493
Size: 711 926 79 1658 2702
Fragments arranged by size:
2702 1658 926 711 79
BsaBI GATnn'nnATC
Cuts at: 6070 6070
Size: 6076
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2259 2808 2978 3011
Size: 53 83 186 936 726 549 170 33
Cuts at: 3011 3306 3883 4265 4900 275
Size: 295 577 382 635 1451
Fragments arranged by size:
1451 936 726 635 577 549 382 295
186 170 83 53 33
BsaWI w'CCGG_w
Cuts at: 4509 4907 5181 5328 5739 4509
Size: 398 274 147 411 4846
Fragments arranged by size:
4846 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2381 2411 2621 2651 3116
Size: 30 165 30 978 30 210 30 465
Cuts at: 3116 3146 1178
Size: 30 4108
Fragments arranged by size:
4108 978 465 210 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2071 3361 1186
Size: 885 1290 3901
Fragments arranged by size:
3901 1290 885
BseYI C'CCAG_C
Cuts at: 2008 2327 3052 5279 5775 2008
Size: 319 725 2227 496 2309
Fragments arranged by size:
2309 2227 725 496 319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2693 1163
Size: 573 957 4546
Fragments arranged by size:
4546 957 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2151 2286 2295 2358 2835 3526
Size: 275 186 150 135 9 63 477 691
Cuts at: 3526 4287 5315 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 691 477 275 186 150
135 63 9
BsiHKAI G_wGCw'C
Cuts at: 2946 4137 4222 5293 2946
Size: 1191 85 1071 3729
Fragments arranged by size:
3729 1191 1071 85
BsiWI C'GTAC_G
Cuts at: 2205 2205
Size: 6076
BsmI GAATG_Cn'
Cuts at: 3587 3680 3587
Size: 93 5983
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2070 2189 2228 2339 2408 2531
Size: 399 244 36 119 39 111 69 123
Cuts at: 2531 2667 2696 2845 2906 2946 2999 3059 3281
Size: 136 29 149 61 40 53 60 222
Cuts at: 3281 3395 4137 4222 5293 1391
Size: 114 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 244 222 149 136
123 119 114 111 85 69 61 60
53 40 39 36 29
BspEI T'CCGG_A
Cuts at: 5739 5739
Size: 6076
BspHI T'CATG_A
Cuts at: 3965 5695 3965
Size: 1730 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2757 3057 3431 844
Size: 1913 300 374 3489
Fragments arranged by size:
3489 1913 374 300
BsrBI CCG'CTC
Cuts at: 1394 1823 2015 2341 2670 3963 1394
Size: 429 192 326 329 1293 3507
Fragments arranged by size:
3507 1293 429 329 326 192
BsrDI GCAATG_nn'
Cuts at: 65 1248 4571 4745 65
Size: 1183 3323 174 1396
Fragments arranged by size:
3323 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 2294 2592 2813 2881 2980 3007
Size: 110 292 192 298 221 68 99 27
Cuts at: 3007 4720 4907 1700
Size: 1713 187 2869
Fragments arranged by size:
2869 1713 298 292 221 192 187 110
99 68 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6076
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6076
BssSI C'ACGA_G
Cuts at: 2033 4136 5148 2033
Size: 2103 1012 2961
Fragments arranged by size:
2961 2103 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6076
BstBI TT'CG_AA
Cuts at: 4880 4880
Size: 6076
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6076
BstYI r'GATC_y
Cuts at: 1972 2556 3406 3773 4158 4175 5627 5713 5725
Size: 584 850 367 385 17 1452 86 12
Cuts at: 5725 6071 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 850 584 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3084 3084
Size: 6076
BtgI C'CryG_G
Cuts at: 513 1065 2154 2262 2364 2511 3039 3273 513
Size: 552 1089 108 102 147 528 234 3316
Fragments arranged by size:
3316 1089 552 528 234 147 108 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5337
Fragments arranged by size:
5337 683 56
BtsI GCAGTG_nn'
Cuts at: 3592 4386 4406 3592
Size: 794 20 5262
Fragments arranged by size:
5262 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6041
DraI TTT'AAA
Cuts at: 3380 3380
Size: 6076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5083 809
Size: 507 3767 1802
Fragments arranged by size:
3767 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2283 2292
Size: 54 1425 50 275 18 318 135 9
Cuts at: 2292 2355 2514 2832 3270 3318 3523 4412 6041
Size: 63 159 318 438 48 205 889 1629
Cuts at: 6041 8
Size: 43
Fragments arranged by size:
1629 1425 889 438 318 318 275 205
159 135 63 54 50 48 43 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 2148 2283 2292 2355 2832 3523 1537
Size: 275 336 135 9 63 477 691 4090
Fragments arranged by size:
4090 691 477 336 275 135 63 9
EarI CTCTTCn'nnn_
Cuts at: 1353 2585 4006 1353
Size: 1232 1421 3423
Fragments arranged by size:
3423 1421 1232
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2615 4663 5037 5183 387
Size: 1064 138 1026 2048 374 146 1280
Fragments arranged by size:
2048 1280 1064 1026 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2678 2813 2990 3116 4139 4736
Size: 393 436 610 135 177 126 1023 597
Cuts at: 4736 5523 5791 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1023 787 610 597 436 393 268
177 135 126
EcoO109I rG'GnC_Cy
Cuts at: 2956 2956
Size: 6076
EcoRI G'AATT_C
Cuts at: 3385 3763 3385
Size: 378 5698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 6076
FseI GG_CCGG'CC
Cuts at: 2885 2885
Size: 6076
FspI TGC'GCA
Cuts at: 4582 4582
Size: 6076
HaeII r_GCGC'y
Cuts at: 1678 2217 2262 2611 2767 2792 2811 2928 2993
Size: 539 45 349 156 25 19 117 65
Cuts at: 2993 3014 5223 1678
Size: 21 2209 2531
Fragments arranged by size:
2531 2209 539 349 156 117 65 45
25 21 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4717 4749 4791 4823 1373
Size: 32 135 32 3145 32 42 32 2626
Fragments arranged by size:
3145 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3420 3666 669
Size: 988 281 1482 246 3079
Fragments arranged by size:
3079 1482 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6076
HpaI GTT'AAC
Cuts at: 3666 3666
Size: 6076
KasI G'GCGC_C
Cuts at: 2258 2807 3010 2258
Size: 549 203 5324
Fragments arranged by size:
5324 549 203
KpnI G_GTAC'C
Cuts at: 3401 3401
Size: 6076
MfeI C'AATT_G
Cuts at: 3675 3675
Size: 6076
MluI A'CGCG_T
Cuts at: 5952 5952
Size: 6076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2067 5190 5374 1305
Size: 762 3123 184 2007
Fragments arranged by size:
3123 2007 762 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2516 3272 10
Size: 54 1425 343 684 756 2814
Fragments arranged by size:
2814 1425 756 684 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2132 2138 2195 2510 4034 4393 4552
Size: 648 966 6 57 315 1524 359 159
Cuts at: 4552 518
Size: 2042
Fragments arranged by size:
2042 1524 966 648 359 315 159 57
6
MspA1I CmG'CkG
Cuts at: 1606 2163 2785 2820 3041 3492 4169 5317 5562
Size: 557 622 35 221 451 677 1148 245
Cuts at: 5562 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 622 557 451 245 221
35
NaeI GCC'GGC
Cuts at: 2104 2296 2815 2883 2982 3009 2104
Size: 192 519 68 99 27 5171
Fragments arranged by size:
5171 519 192 99 68 27
NarI GG'CG_CC
Cuts at: 2259 2808 3011 2259
Size: 549 203 5324
Fragments arranged by size:
5324 549 203
NcoI C'CATG_G
Cuts at: 513 1065 2154 2262 2364 2511 3273 513
Size: 552 1089 108 102 147 762 3316
Fragments arranged by size:
3316 1089 762 552 147 108 102
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6076
NgoMIV G'CCGG_C
Cuts at: 2102 2294 2813 2881 2980 3007 2102
Size: 192 519 68 99 27 5171
Fragments arranged by size:
5171 519 192 99 68 27
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6076
NotI GC'GGCC_GC
Cuts at: 2148 2832 3523 2148
Size: 684 691 4701
Fragments arranged by size:
4701 691 684
NspI r_CATG'y
Cuts at: 1132 1229 2143 2200 2731 3434 4979 1132
Size: 97 914 57 531 703 1545 2229
Fragments arranged by size:
2229 1545 914 703 531 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6076
PciI A'CATG_T
Cuts at: 2196 2727 4975 2196
Size: 531 2248 3297
Fragments arranged by size:
3297 2248 531
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3052 3202 1108
Size: 1944 150 3982
Fragments arranged by size:
3982 1944 150
PmeI GTTT'AAAC
Cuts at: 3380 3380
Size: 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 5658 5690 4145
Size: 32 1481 32 4531
Fragments arranged by size:
4531 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6076
PsiI TTA'TAA
Cuts at: 3646 3646
Size: 6076
PspOMI G'GGCC_C
Cuts at: 3055 3277 3055
Size: 222 5854
PspXI vC'TCGA_Gb
Cuts at: 2920 2920
Size: 6076
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2729 2761 2729
Size: 32 6044
PstI C_TGCA'G
Cuts at: 830 3428 830
Size: 2598 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6076
PvuII CAG'CTG
Cuts at: 2785 2785
Size: 6076
SacII CC_GC'GG
Cuts at: 3042 3042
Size: 6076
SalI G'TCGA_C
Cuts at: 1936 3418 1936
Size: 1482 4594
SbfI CC_TGCA'GG
Cuts at: 3428 3428
Size: 6076
ScaI AGT'ACT
Cuts at: 4324 6036 4324
Size: 1712 4364
SfcI C'TryA_G
Cuts at: 826 1045 2455 3424 4559 5240 5431 826
Size: 219 1410 969 1135 681 191 1471
Fragments arranged by size:
1471 1410 1135 969 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2291 2291
Size: 6076
SfoI GGC'GCC
Cuts at: 2260 2809 3012 2260
Size: 549 203 5324
Fragments arranged by size:
5324 549 203
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6076
SmaI CCC'GGG
Cuts at: 1872 3022 3072 3403 1872
Size: 1150 50 331 4545
Fragments arranged by size:
4545 1150 331 50
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2320 2920 3209 4180 5081 5343
Size: 197 926 377 600 289 971 901 262
Cuts at: 5343 820
Size: 1553
Fragments arranged by size:
1553 971 926 901 600 377 289 262
197
SnaBI TAC'GTA
Cuts at: 493 3867 493
Size: 3374 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6076
SphI G_CATG'C
Cuts at: 1132 3434 1132
Size: 2302 3774
SspI AAT'ATT
Cuts at: 5 52 4000 5
Size: 47 3948 2081
Fragments arranged by size:
3948 2081 47
StuI AGG'CCT
Cuts at: 1517 2849 3215 1517
Size: 1332 366 4378
Fragments arranged by size:
4378 1332 366
StyI C'CwwG_G
Cuts at: 513 1065 2154 2262 2364 2511 3273 3515 513
Size: 552 1089 108 102 147 762 242 3074
Fragments arranged by size:
3074 1089 762 552 242 147 108 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4103 4120 4273 4458 4903 325
Size: 1255 2523 17 153 185 445 1498
Fragments arranged by size:
2523 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3348 4322 6034
Size: 274 80 33 51 1443 1371 974 1712
Cuts at: 6034 96
Size: 138
Fragments arranged by size:
1712 1443 1371 974 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2024 2056 2621 2653 3116 3148 6036 6068 2024
Size: 32 565 32 463 32 2888 32 2032
Fragments arranged by size:
2888 2032 565 463 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6076
XbaI T'CTAG_A
Cuts at: 3412 3412
Size: 6076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 2371 2269
Size: 102 5974
XhoI C'TCGA_G
Cuts at: 1943 2920 1943
Size: 977 5099
XmaI C'CCGG_G
Cuts at: 1870 3020 3070 3401 1870
Size: 1150 50 331 4545
Fragments arranged by size:
4545 1150 331 50
XmnI GAAnn'nnTTC
Cuts at: 2765 4205 2765
Size: 1440 4636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3884 276
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsmBI BstXI
BstZ17I DraIII EcoICRI EcoNI EcoRV FspAI NruI NsiI
PacI PfoI PmlI PpuMI RsrII SacI SanDI SapI
SexAI SrfI SwaI