Restriction map for
pFN21AB7037
(Circular) (Six-base) MAPSORT of: pFN21AB7037.seq Check: 6816 from: 1 to: 6265
pFN21AB7037 6265 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 2238 2053
Size: 185 6080
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2993 4328 5712 5980 1632
Size: 1361 1335 1384 268 1917
Fragments arranged by size:
1917 1384 1361 1335 268
AfeI AGC'GCT
Cuts at: 3200 3200
Size: 6265
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 5164 6141 1203
Size: 3961 977 1327
Fragments arranged by size:
3961 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 2045 2511 4008 5580 2045
Size: 466 1497 1572 2730
Fragments arranged by size:
2730 1572 1497 466
ApaI G_GGCC'C
Cuts at: 3419 3419
Size: 6265
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 3574 3800 3952 6061 1493
Size: 2081 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3306 3306
Size: 6265
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 3092 3286 3338 3412 3449 3458
Size: 73 155 994 194 52 74 37 9
Cuts at: 3458 3554 3590 1870
Size: 96 36 4545
Fragments arranged by size:
4545 994 194 155 96 74 73 52
37 36 9
BamHI G'GATC_C
Cuts at: 2766 3595 3962 2766
Size: 829 367 5069
Fragments arranged by size:
5069 829 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2226 2566 3096 3349
Size: 325 217 627 375 64 340 530 253
Cuts at: 3349 3356 3473 3550 3586 5041 618
Size: 7 117 77 36 1455 1842
Fragments arranged by size:
1842 1455 627 530 375 340 325 253
217 117 77 64 36 7
BanII G_rGCy'C
Cuts at: 1391 2636 3287 3387 3419 3450 3458 3584 1391
Size: 1245 651 100 32 31 8 126 4072
Fragments arranged by size:
4072 1245 651 126 100 32 31 8
BbeI G_GCGC'C
Cuts at: 2166 3353 3360 3477 3554 2166
Size: 1187 7 117 77 4877
Fragments arranged by size:
4877 1187 117 77 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2884 2918 4456 4490 1903
Size: 34 947 34 1538 34 3678
Fragments arranged by size:
3678 1538 947 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2472 4157 5367 2472
Size: 1685 1210 3370
Fragments arranged by size:
3370 1685 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2495 3150 3249
Size: 107 122 71 631 605 823 655 99
Cuts at: 3249 3399 3509 4876 136
Size: 150 110 1367 1525
Fragments arranged by size:
1525 1367 823 655 631 605 150 122
110 107 99 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 3419 4326 5482 1790
Size: 1629 907 1156 2573
Fragments arranged by size:
2573 1629 1156 907
BmgBI CAC'GTC
Cuts at: 1318 3508 1318
Size: 2190 4075
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2062 2344 3996
Size: 416 53 83 399 24 645 282 1652
Cuts at: 3996 4954 442
Size: 958 1753
Fragments arranged by size:
1753 1652 958 645 416 399 282 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2848 3040 3215 4925 1239
Size: 1609 192 175 1710 2579
Fragments arranged by size:
2579 1710 1609 192 175
Bpu10I CC'TnA_GC
Cuts at: 2798 3245 3365 3381 2798
Size: 447 120 16 5682
Fragments arranged by size:
5682 447 120 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4390 5255 5553 4390
Size: 865 298 5102
Fragments arranged by size:
5102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 2237 2985 3127
Size: 53 83 186 936 630 74 748 142
Cuts at: 3127 3350 3357 3474 3551 4072 4454 5089 275
Size: 223 7 117 77 521 382 635 1451
Fragments arranged by size:
1451 936 748 635 630 521 382 223
186 142 117 83 77 74 53 7
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3038 3068 1178
Size: 30 165 30 1635 30 4375
Fragments arranged by size:
4375 1635 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2614 1186
Size: 1428 4837
BseYI C'CCAG_C
Cuts at: 2118 2158 2341 3010 5468 5964 2118
Size: 40 183 669 2458 496 2419
Fragments arranged by size:
2458 2419 669 496 183 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2374 1163
Size: 573 638 5054
Fragments arranged by size:
5054 638 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2431 3112 3118 3173 3394 3403
Size: 275 186 430 681 6 55 221 9
Cuts at: 3403 3504 3715 4476 5504 1540
Size: 101 211 761 1028 2301
Fragments arranged by size:
2301 1028 761 681 430 275 221 211
186 101 55 9 6
BsiHKAI G_wGCw'C
Cuts at: 2328 4326 4411 5482 2328
Size: 1998 85 1071 3111
Fragments arranged by size:
3111 1998 1071 85
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2636 3287 3387 3419 3450 3458
Size: 399 538 308 651 100 32 31 8
Cuts at: 3458 3584 4326 4411 5482 1391
Size: 126 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 538 399 308 126
100 85 32 31 8
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 2727 4154 5884 2727
Size: 1427 1730 3108
Fragments arranged by size:
3108 1730 1427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 2238 2286 3620 844
Size: 1209 185 48 1334 3489
Fragments arranged by size:
3489 1334 1209 185 48
BsrBI CCG'CTC
Cuts at: 1394 1823 3249 3421 4152 1394
Size: 429 1426 172 731 3507
Fragments arranged by size:
3507 1426 731 429 172
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2024 2223 2563 3168 4909 5096 1700
Size: 110 214 199 340 605 1741 187 2869
Fragments arranged by size:
2869 1741 605 340 214 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 3073 3306 1925
Size: 1148 233 4884
Fragments arranged by size:
4884 1148 233
BssSI C'ACGA_G
Cuts at: 2786 4325 5337 2786
Size: 1539 1012 3714
Fragments arranged by size:
3714 1539 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6265
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 2473 2593 1184
Size: 1289 120 4856
Fragments arranged by size:
4856 1289 120
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2146
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 2349 2766 3595 3962 4347 4364 5816 5902
Size: 377 417 829 367 385 17 1452 86
Cuts at: 5902 5914 6260 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 829 417 385 377 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2762 2762
Size: 6265
BtgI C'CryG_G
Cuts at: 513 1065 2268 2373 3106 3112 3173 3301 3397
Size: 552 1203 105 733 6 61 128 96
Cuts at: 3397 3498 513
Size: 101 3280
Fragments arranged by size:
3280 1203 733 552 128 105 101 96
61 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2923 487
Size: 683 56 1697 3829
Fragments arranged by size:
3829 1697 683 56
BtsI GCAGTG_nn'
Cuts at: 2111 3781 4575 4595 2111
Size: 1670 794 20 3781
Fragments arranged by size:
3781 1670 794 20
ClaI AT'CG_AT
Cuts at: 1554 2427 1554
Size: 873 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2265 2271
Size: 54 1425 50 275 18 196 239 6
Cuts at: 2271 2691 3109 3115 3170 3391 3400 3501 3510
Size: 420 418 6 55 221 9 101 9
Cuts at: 3510 3712 4601 6230 8
Size: 202 889 1629 43
Fragments arranged by size:
1629 1425 889 420 418 275 239 221
202 196 101 55 54 50 43 18
9 9 6 6
EagI C'GGCC_G
Cuts at: 1537 1812 3109 3115 3170 3391 3400 3501 3712
Size: 275 1297 6 55 221 9 101 211
Cuts at: 3712 1537
Size: 4090
Fragments arranged by size:
4090 1297 275 221 211 101 55 9
6
EarI CTCTTCn'nnn_
Cuts at: 1353 2624 3428 4195 1353
Size: 1271 804 767 3423
Fragments arranged by size:
3423 1271 804 767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2362 4852 5226 5372 387
Size: 1064 138 773 2490 374 146 1280
Fragments arranged by size:
2490 1280 1064 773 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2848 2993 3040 3215 4328 4925 5712
Size: 393 1216 145 47 175 1113 597 787
Cuts at: 5712 5980 1239
Size: 268 1524
Fragments arranged by size:
1524 1216 1113 787 597 393 268 175
145 47
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 6265
FspI TGC'GCA
Cuts at: 3086 4771 3086
Size: 1685 4580
FspAI rTGC'GCAy
Cuts at: 3086 3086
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2166 2501 3202 3353 3360 3477 3554 5412
Size: 488 335 701 151 7 117 77 1858
Cuts at: 5412 1678
Size: 2531
Fragments arranged by size:
2531 1858 701 488 335 151 117 77
7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4906 4938 4980 5012 1373
Size: 32 135 32 3334 32 42 32 2626
Fragments arranged by size:
3334 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 3609 3855 669
Size: 988 281 322 1349 246 3079
Fragments arranged by size:
3079 1349 988 322 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KasI G'GCGC_C
Cuts at: 2162 3349 3356 3473 3550 2162
Size: 1187 7 117 77 4877
Fragments arranged by size:
4877 1187 117 77 7
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2766 5379 5563 1305
Size: 1461 2613 184 2007
Fragments arranged by size:
2613 2007 1461 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2267 2693 3512 10
Size: 54 1425 343 435 426 819 2763
Fragments arranged by size:
2763 1425 819 435 426 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2441 4223 4582 4741 518
Size: 648 978 297 1782 359 159 2042
Fragments arranged by size:
2042 1782 978 648 359 297 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2312 3027 3108 3114 3234 3240 3259
Size: 516 190 715 81 6 120 6 19
Cuts at: 3259 3303 3399 3500 3681 4358 5506 5751 1606
Size: 44 96 101 181 677 1148 245 2120
Fragments arranged by size:
2120 1148 715 677 516 245 190 181
120 101 96 81 44 19 6 6
NaeI GCC'GGC
Cuts at: 2565 2565
Size: 6265
NarI GG'CG_CC
Cuts at: 2163 3350 3357 3474 3551 2163
Size: 1187 7 117 77 4877
Fragments arranged by size:
4877 1187 117 77 7
NcoI C'CATG_G
Cuts at: 513 1065 2268 513
Size: 552 1203 4510
Fragments arranged by size:
4510 1203 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NgoMIV G'CCGG_C
Cuts at: 2563 2563
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3109 3115 3400 3712 3109
Size: 6 285 312 5662
Fragments arranged by size:
5662 312 285 6
NspI r_CATG'y
Cuts at: 1132 1229 3544 3623 5168 1132
Size: 97 2315 79 1545 2229
Fragments arranged by size:
2315 2229 1545 97 79
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspOMI G'GGCC_C
Cuts at: 3415 3415
Size: 6265
PspXI vC'TCGA_Gb
Cuts at: 2098 2098
Size: 6265
PstI C_TGCA'G
Cuts at: 830 2460 3617 830
Size: 1630 1157 3478
Fragments arranged by size:
3478 1630 1157
PvuI CG_AT'CG
Cuts at: 2001 2431 2001
Size: 430 5835
PvuII CAG'CTG
Cuts at: 2122 3027 3240 2122
Size: 905 213 5147
Fragments arranged by size:
5147 905 213
SacII CC_GC'GG
Cuts at: 3109 3115 3304 3400 3501 3109
Size: 6 189 96 101 5873
Fragments arranged by size:
5873 189 101 96 6
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SapI GCTCTTCn'nnn_
Cuts at: 3428 3428
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 2456 3613 4748 5429 5620 826
Size: 219 1411 1157 1135 681 191 1471
Fragments arranged by size:
1471 1411 1157 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3399 3509 3399
Size: 110 6155
SfoI GGC'GCC
Cuts at: 2164 3351 3358 3475 3552 2164
Size: 1187 7 117 77 4877
Fragments arranged by size:
4877 1187 117 77 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3288 3460 3556 3592 1872
Size: 1416 172 96 36 4545
Fragments arranged by size:
4545 1416 172 96 36
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2098 4369 5270 5532 820
Size: 197 926 155 2271 901 262 1553
Fragments arranged by size:
2271 1553 926 901 262 197 155
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SrfI GCCC'GGGC
Cuts at: 3288 3556 3288
Size: 268 5997
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6265
StyI C'CwwG_G
Cuts at: 513 1065 2268 2548 2748 2808 2814 3704 513
Size: 552 1203 280 200 60 6 890 3074
Fragments arranged by size:
3074 1203 890 552 280 200 60 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2326 2541 2584 3458 3479 4292 4309
Size: 1255 746 215 43 874 21 813 17
Cuts at: 4309 4462 4647 5092 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 874 813 746 445 215 185
153 43 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2670 4511 6223
Size: 274 80 33 51 1443 693 1841 1712
Cuts at: 6223 96
Size: 138
Fragments arranged by size:
1841 1712 1443 693 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2341 2369 2373 2401 6225 6257 2341
Size: 28 4 28 3824 32 2349
Fragments arranged by size:
3824 2349 32 28 28 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 2098 1943
Size: 155 6110
XmaI C'CCGG_G
Cuts at: 1870 3286 3458 3554 3590 1870
Size: 1416 172 96 36 4545
Fragments arranged by size:
4545 1416 172 96 36
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI NruI
NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI
SanDI SexAI SwaI XcmI