Restriction map for
pFN21AB7035
(Circular) (Six-base) MAPSORT of: pFN21AB7035.seq Check: 1693 from: 1 to: 5983
pFN21AB7035 5983 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2637 3326 1937
Size: 700 689 4594
Fragments arranged by size:
4594 700 689
Acc65I G'GTAC_C
Cuts at: 2236 3304 2236
Size: 1068 4915
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2925 4046 5430 5698 1632
Size: 1293 1121 1384 268 1917
Fragments arranged by size:
1917 1384 1293 1121 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 2110 2222 4712 2110
Size: 112 2490 3381
Fragments arranged by size:
3381 2490 112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AlwNI CAG_nnn'CTG
Cuts at: 3726 5298 3726
Size: 1572 4411
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 3292 3518 3670 5779 1493
Size: 1799 226 152 2109 1697
Fragments arranged by size:
2109 1799 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 3067 3308 1870
Size: 73 1124 241 4545
Fragments arranged by size:
4545 1124 241 73
BamHI G'GATC_C
Cuts at: 2078 3313 3680 2078
Size: 1235 367 4381
Fragments arranged by size:
4381 1235 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 2756 3304 4759 618
Size: 325 217 627 449 520 548 1455 1842
Fragments arranged by size:
1842 1455 627 548 520 449 325 217
BanII G_rGCy'C
Cuts at: 1391 2714 3302 1391
Size: 1323 588 4072
Fragments arranged by size:
4072 1323 588
BbeI G_GCGC'C
Cuts at: 2760 2760
Size: 5983
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2411 2656 2913 928
Size: 971 512 245 257 3998
Fragments arranged by size:
3998 971 512 257 245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4174 4208 1903
Size: 34 50 34 2153 34 3678
Fragments arranged by size:
3678 2153 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4594 136
Size: 107 122 71 631 605 2922 1525
Fragments arranged by size:
2922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2091 3068 3183 4044 5200 1790
Size: 301 977 115 861 1156 2573
Fragments arranged by size:
2573 1156 977 861 301 115
BmgBI CAC'GTC
Cuts at: 1318 3087 1318
Size: 1769 4214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3714 4672 442
Size: 416 53 83 399 24 2297 958 1753
Fragments arranged by size:
2297 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2659 4643 1239
Size: 1420 1984 2579
Fragments arranged by size:
2579 1984 1420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4108 4973 5271 4108
Size: 865 298 4820
Fragments arranged by size:
4820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2209 3187 4646 882
Size: 617 710 978 1459 2219
Fragments arranged by size:
2219 1459 978 710 617
BsaAI yAC'GTr
Cuts at: 493 1204 3774 493
Size: 711 2570 2702
Fragments arranged by size:
2702 2570 711
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2757 2926 3790 4172
Size: 53 83 186 936 1224 169 864 382
Cuts at: 4172 4807 275
Size: 635 1451
Fragments arranged by size:
1451 1224 936 864 635 382 186 169
83 53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 3260 3290 1178
Size: 30 165 30 882 30 945 30 3871
Fragments arranged by size:
3871 945 882 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 2620 2813 3281 1186
Size: 888 546 193 468 3888
Fragments arranged by size:
3888 888 546 468 193
BseYI C'CCAG_C
Cuts at: 2229 5186 5682 2229
Size: 2957 496 2530
Fragments arranged by size:
2957 2530 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2396 2481 1163
Size: 573 450 210 85 4665
Fragments arranged by size:
4665 573 450 210 85
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2654 2714 3281 4044 4129 5200 2654
Size: 60 567 763 85 1071 3437
Fragments arranged by size:
3437 1071 763 567 85 60
BsmI GAATG_Cn'
Cuts at: 3494 3587 3494
Size: 93 5890
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2654 2714 3068 3183 3281 3302
Size: 399 301 563 60 354 115 98 21
Cuts at: 3302 4044 4129 5200 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 563 399 354 301 115
98 85 60 21
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3338 844
Size: 2494 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3021 4478 4652 65
Size: 1183 1773 1457 174 1396
Fragments arranged by size:
1773 1457 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2233 2922 4627 4814 1700
Size: 110 423 689 1705 187 2869
Fragments arranged by size:
2869 1705 689 423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5983
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2753 2753
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 2078 3313 3680 4065 4082 5534 5620 5632
Size: 106 1235 367 385 17 1452 86 12
Cuts at: 5632 5978 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1235 385 367 346 106 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5431
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2903 487
Size: 683 56 1677 3567
Fragments arranged by size:
3567 1677 683 56
BtsI GCAGTG_nn'
Cuts at: 2443 2747 3499 4293 4313 2443
Size: 304 752 794 20 4113
Fragments arranged by size:
4113 794 752 304 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 2193 3287 2193
Size: 1094 4889
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 3430 4319
Size: 54 1425 50 275 18 294 1306 889
Cuts at: 4319 5948 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1306 889 294 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 2480 2534 2568 2642 2790 3913 1353
Size: 854 273 54 34 74 148 1123 3423
Fragments arranged by size:
3423 1123 854 273 148 74 54 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2513 2838 4570 4944 5090 387
Size: 1064 138 924 325 1732 374 146 1280
Fragments arranged by size:
1732 1280 1064 924 374 325 146 138
EcoICRI GAG'CTC
Cuts at: 2712 2712
Size: 5983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2659 2925 4046 4643 5430 5698 1239
Size: 393 1027 266 1121 597 787 268 1524
Fragments arranged by size:
1524 1121 1027 787 597 393 268 266
EcoNI CCTnn'n_nnAGG
Cuts at: 2135 2135
Size: 5983
EcoO109I rG'GnC_Cy
Cuts at: 3093 3093
Size: 5983
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 2252 2760 5130 1678
Size: 574 508 2370 2531
Fragments arranged by size:
2531 2370 574 508
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2208 2240 2865
Size: 32 135 32 524 32 80 32 625
Cuts at: 2865 2897 3010 3042 4624 4656 4698 4730 1373
Size: 32 113 32 1582 32 42 32 2626
Fragments arranged by size:
2626 1582 625 524 135 113 80 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2959 3327 3573 669
Size: 988 281 1021 368 246 3079
Fragments arranged by size:
3079 1021 988 368 281 246
HindIII A'AGCT_T
Cuts at: 748 2270 748
Size: 1522 4461
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KasI G'GCGC_C
Cuts at: 2756 2756
Size: 5983
KpnI G_GTAC'C
Cuts at: 2240 3308 2240
Size: 1068 4915
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5097 5281 1305
Size: 3792 184 2007
Fragments arranged by size:
3792 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4161
Fragments arranged by size:
4161 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3203 3260 3941 4300 4459 518
Size: 648 2037 57 681 359 159 2042
Fragments arranged by size:
2042 2037 681 648 359 159 57
MspA1I CmG'CkG
Cuts at: 1606 2689 3399 4076 5224 5469 1606
Size: 1083 710 677 1148 245 2120
Fragments arranged by size:
2120 1148 1083 710 677 245
NaeI GCC'GGC
Cuts at: 2924 2924
Size: 5983
NarI GG'CG_CC
Cuts at: 2757 2757
Size: 5983
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5431
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NgoMIV G'CCGG_C
Cuts at: 2922 2922
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 2200 2663 3139 3341 4886 1132
Size: 97 971 463 476 202 1545 2229
Fragments arranged by size:
2229 1545 971 476 463 202 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5983
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5983
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2604 2636 4052 4084 5565 5597 2604
Size: 32 1416 32 1481 32 2990
Fragments arranged by size:
2990 1481 1416 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3093 3093
Size: 5983
PshAI GACnn'nnGTC
Cuts at: 1613 2513 3199 1613
Size: 900 686 4397
Fragments arranged by size:
4397 900 686
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PstI C_TGCA'G
Cuts at: 830 2892 3335 830
Size: 2062 443 3478
Fragments arranged by size:
3478 2062 443
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
SacI G_AGCT'C
Cuts at: 2714 2714
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SapI GCTCTTCn'nnn_
Cuts at: 2480 2642 2480
Size: 162 5821
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 2888 3331 4466 5147 5338 826
Size: 219 1843 443 1135 681 191 1471
Fragments arranged by size:
1843 1471 1135 681 443 219 191
SfoI GGC'GCC
Cuts at: 2758 2758
Size: 5983
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 2922 1700
Size: 1222 4761
SmaI CCC'GGG
Cuts at: 1872 3310 1872
Size: 1438 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4087 4988 5250 820
Size: 197 926 2144 901 262 1553
Fragments arranged by size:
2144 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3139 3341 1132
Size: 2007 202 3774
Fragments arranged by size:
3774 2007 202
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5983
StyI C'CwwG_G
Cuts at: 513 1065 2241 2427 2490 3422 513
Size: 552 1176 186 63 932 3074
Fragments arranged by size:
3074 1176 932 552 186 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2243 3264 4010 4027 4180 4365 4810
Size: 1255 663 1021 746 17 153 185 445
Cuts at: 4810 325
Size: 1498
Fragments arranged by size:
1498 1255 1021 746 663 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3210 4229 5941
Size: 274 80 33 51 1443 1233 1019 1712
Cuts at: 5941 96
Size: 138
Fragments arranged by size:
1712 1443 1233 1019 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2719 2751 3090 3122 3260 3292 5943 5975 2719
Size: 32 339 32 138 32 2651 32 2727
Fragments arranged by size:
2727 2651 339 138 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 3308 1870
Size: 1438 4545
XmnI GAAnn'nnTTC
Cuts at: 2978 4112 2978
Size: 1134 4849
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI
SfiI SrfI SwaI XcmI