Restriction map for
pFN21AB7024
(Circular) (Six-base) MAPSORT of: pFN21AB7024.seq Check: 3054 from: 1 to: 5983
pFN21AB7024 5983 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2300 2333 4046 5430 5698 1632
Size: 668 33 1713 1384 268 1917
Fragments arranged by size:
1917 1713 1384 668 268 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 2785 1166
Size: 1619 4364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2216 1184
Size: 1032 4951
AlwNI CAG_nnn'CTG
Cuts at: 2687 3246 3726 5298 2687
Size: 559 480 1572 3372
Fragments arranged by size:
3372 1572 559 480
ApaI G_GGCC'C
Cuts at: 2417 2417
Size: 5983
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 3292 3518 3670 5779 1493
Size: 1799 226 152 2109 1697
Fragments arranged by size:
2109 1799 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 2167 2416 2567 3308 1870
Size: 73 224 249 151 741 4545
Fragments arranged by size:
4545 741 249 224 151 73
BamHI G'GATC_C
Cuts at: 3313 3680 3313
Size: 367 5616
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 2246 2264 2422 3304
Size: 325 217 627 420 39 18 158 882
Cuts at: 3304 4759 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 882 627 420 325 217 158
39 18
BanII G_rGCy'C
Cuts at: 1391 2417 2850 3302 1391
Size: 1026 433 452 4072
Fragments arranged by size:
4072 1026 452 433
BbeI G_GCGC'C
Cuts at: 2250 2268 2426 2250
Size: 18 158 5807
Fragments arranged by size:
5807 158 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2819 928
Size: 971 920 4092
Fragments arranged by size:
4092 971 920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4174 4208 1903
Size: 34 2237 34 3678
Fragments arranged by size:
3678 2237 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2255 4594 136
Size: 107 122 71 631 605 583 2339 1525
Fragments arranged by size:
2339 1525 631 605 583 122 107 71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2417 4044 5200 1790
Size: 627 1627 1156 2573
Fragments arranged by size:
2573 1627 1156 627
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2812 3714 4672
Size: 416 53 83 399 24 1395 902 958
Cuts at: 4672 442
Size: 1753
Fragments arranged by size:
1753 1395 958 902 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 2324 2348 2623 2783 3043 4643 1239
Size: 826 259 24 275 160 260 1600 2579
Fragments arranged by size:
2579 1600 826 275 260 259 160 24
Bpu10I CC'TnA_GC
Cuts at: 2597 2597
Size: 5983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 4108 4973 5271 2288
Size: 1820 865 298 3000
Fragments arranged by size:
3000 1820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4646 882
Size: 617 3147 2219
Fragments arranged by size:
3147 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3774 493
Size: 711 2570 2702
Fragments arranged by size:
2702 2570 711
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2038 2247 2265 2423
Size: 53 83 186 936 505 209 18 158
Cuts at: 2423 3790 4172 4807 275
Size: 1367 382 635 1451
Fragments arranged by size:
1451 1367 936 635 505 382 209 186
158 83 53 18
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2038 2068 3041 3071 1178
Size: 30 165 30 635 30 973 30 4090
Fragments arranged by size:
4090 973 635 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2062 2090 2093 2115 2118 2822 1186
Size: 876 28 3 22 3 704 4347
Fragments arranged by size:
4347 876 704 28 22 3 3
BseYI C'CCAG_C
Cuts at: 2410 3210 5186 5682 2410
Size: 800 1976 496 2711
Fragments arranged by size:
2711 1976 800 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 3209 1163
Size: 573 1218 255 3937
Fragments arranged by size:
3937 1218 573 255
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2259 2395 3433 4194 5222 1540
Size: 275 186 258 136 1038 761 1028 2301
Fragments arranged by size:
2301 1038 1028 761 275 258 186 136
BsiHKAI G_wGCw'C
Cuts at: 2304 4044 4129 5200 2304
Size: 1740 85 1071 3087
Fragments arranged by size:
3087 1740 1071 85
BsmI GAATG_Cn'
Cuts at: 2512 3494 3587 2512
Size: 982 93 4908
Fragments arranged by size:
4908 982 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2045 2045
Size: 5983
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2417 2850 3302 4044 4129 5200
Size: 399 514 113 433 452 742 85 1071
Cuts at: 5200 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 514 452 433 399 113
85
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 3872 5602 3872
Size: 1730 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3338 844
Size: 2494 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 3870 1394
Size: 429 497 1550 3507
Fragments arranged by size:
3507 1550 497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4478 4652 65
Size: 1183 3230 174 1396
Fragments arranged by size:
3230 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2153 2249 2429 4627 4814 1700
Size: 110 343 96 180 2198 187 2869
Fragments arranged by size:
2869 2198 343 187 180 110 96
BsrGI T'GTAC_A
Cuts at: 96 3000 96
Size: 2904 3079
BssHII G'CGCG_C
Cuts at: 1925 2433 1925
Size: 508 5475
BssSI C'ACGA_G
Cuts at: 3152 4043 5055 3152
Size: 891 1012 4080
Fragments arranged by size:
4080 1012 891
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2299 2376 2941 794
Size: 1505 77 565 3836
Fragments arranged by size:
3836 1505 565 77
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 2216 1184
Size: 1032 4951
BstYI r'GATC_y
Cuts at: 1972 2275 2503 3313 3680 4065 4082 5534 5620
Size: 303 228 810 367 385 17 1452 86
Cuts at: 5620 5632 5978 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 810 385 367 346 303 228
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2877 2877
Size: 5983
BtgI C'CryG_G
Cuts at: 513 1065 2241 2346 2356 3170 513
Size: 552 1176 105 10 814 3326
Fragments arranged by size:
3326 1176 814 552 105 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 3499 4293 4313 3499
Size: 794 20 5169
Fragments arranged by size:
5169 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DraIII CAC_nnn'GTG
Cuts at: 2299 2376 3082 2299
Size: 77 706 5200
Fragments arranged by size:
5200 706 77
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2392 2498
Size: 54 1425 50 275 18 426 136 106
Cuts at: 2498 3430 4319 5948 8
Size: 932 889 1629 43
Fragments arranged by size:
1629 1425 932 889 426 275 136 106
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2256 2392 3430 1537
Size: 275 444 136 1038 4090
Fragments arranged by size:
4090 1038 444 275 136
EarI CTCTTCn'nnn_
Cuts at: 1353 3913 1353
Size: 2560 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2288 3025 4570 4944 5090 387
Size: 1064 138 699 737 1545 374 146 1280
Fragments arranged by size:
1545 1280 1064 737 699 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2300 2324 2333 2348 2623 2783
Size: 393 433 235 24 9 15 275 160
Cuts at: 2783 3043 4046 4643 5430 5698 1239
Size: 260 1003 597 787 268 1524
Fragments arranged by size:
1524 1003 787 597 433 393 275 268
260 235 160 24 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 5983
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
EcoRV GAT'ATC
Cuts at: 2860 2860
Size: 5983
FspI TGC'GCA
Cuts at: 2445 4489 2445
Size: 2044 3939
HaeII r_GCGC'y
Cuts at: 1678 2250 2268 2288 2426 5130 1678
Size: 572 18 20 138 2704 2531
Fragments arranged by size:
2704 2531 572 138 20 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2013 2045 4624 4656 4698
Size: 32 135 32 441 32 2579 32 42
Cuts at: 4698 4730 1373
Size: 32 2626
Fragments arranged by size:
2626 2579 441 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KasI G'GCGC_C
Cuts at: 2246 2264 2422 2246
Size: 18 158 5807
Fragments arranged by size:
5807 158 18
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5097 5281 1305
Size: 3792 184 2007
Fragments arranged by size:
3792 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4161
Fragments arranged by size:
4161 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2785 3941 4300 4459 518
Size: 648 1619 1156 359 159 2042
Fragments arranged by size:
2042 1619 1156 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2243 2326 2358 2702 2929 2971 3139 3246
Size: 637 83 32 344 227 42 168 107
Cuts at: 3246 3399 4076 5224 5469 1606
Size: 153 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 637 344 245 227 168
153 107 83 42 32
NaeI GCC'GGC
Cuts at: 2251 2431 2251
Size: 180 5803
NarI GG'CG_CC
Cuts at: 2247 2265 2423 2247
Size: 18 158 5807
Fragments arranged by size:
5807 158 18
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5431
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NgoMIV G'CCGG_C
Cuts at: 2249 2429 2249
Size: 180 5803
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 3341 4886 1132
Size: 97 2112 1545 2229
Fragments arranged by size:
2229 2112 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2732 1348
Size: 1384 4599
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2347 1108
Size: 1239 4744
PfoI T'CCnGG_A
Cuts at: 2841 3059 2841
Size: 218 5765
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 5565 5597 4052
Size: 32 1481 32 4438
Fragments arranged by size:
4438 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PspOMI G'GGCC_C
Cuts at: 2413 2413
Size: 5983
PstI C_TGCA'G
Cuts at: 830 2408 2674 3138 3335 830
Size: 1578 266 464 197 3478
Fragments arranged by size:
3478 1578 464 266 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
PvuII CAG'CTG
Cuts at: 2326 2929 2971 3246 2326
Size: 603 42 275 5063
Fragments arranged by size:
5063 603 275 42
SacII CC_GC'GG
Cuts at: 2244 2359 2244
Size: 115 5868
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 2404 2670 3134 3331 4466 5147 5338
Size: 219 1359 266 464 197 1135 681 191
Cuts at: 5338 826
Size: 1471
Fragments arranged by size:
1471 1359 1135 681 464 266 219 197
191
SfoI GGC'GCC
Cuts at: 2248 2266 2424 2248
Size: 18 158 5807
Fragments arranged by size:
5807 158 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 2249 2429 1700
Size: 549 180 5254
Fragments arranged by size:
5254 549 180
SmaI CCC'GGG
Cuts at: 1872 2169 2418 3310 1872
Size: 297 249 892 4545
Fragments arranged by size:
4545 892 297 249
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2303 4087 4988 5250 820
Size: 197 926 360 1784 901 262 1553
Fragments arranged by size:
1784 1553 926 901 360 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SrfI GCCC'GGGC
Cuts at: 2169 2169
Size: 5983
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5983
StyI C'CwwG_G
Cuts at: 513 1065 3422 513
Size: 552 2357 3074
Fragments arranged by size:
3074 2357 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2766 2986 4010 4027 4180 4365 4810
Size: 1255 1186 220 1024 17 153 185 445
Cuts at: 4810 325
Size: 1498
Fragments arranged by size:
1498 1255 1186 1024 445 220 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3000 4229 5941
Size: 274 80 33 51 1443 1023 1229 1712
Cuts at: 5941 96
Size: 138
Fragments arranged by size:
1712 1443 1229 1023 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2038 2070 2978 3010 5943 5975 2038
Size: 32 908 32 2933 32 2046
Fragments arranged by size:
2933 2046 908 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2647 3067 2647
Size: 420 5563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 2167 2416 3308 1870
Size: 297 249 892 4545
Fragments arranged by size:
4545 892 297 249
XmnI GAAnn'nnTTC
Cuts at: 4112 4112
Size: 5983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I EcoICRI EcoO109I FalI FseI
FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SwaI