Restriction map for
pFN21AB7023
(Circular) (Six-base) MAPSORT of: pFN21AB7023.seq Check: 8182 from: 1 to: 8023
pFN21AB7023 8023 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2882 3832 4909 2882
Size: 950 1077 5996
Fragments arranged by size:
5996 1077 950
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4287 5833 278
Size: 53 83 186 936 2751 1546 2468
Fragments arranged by size:
2751 2468 1546 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2622 5366 1937
Size: 685 2744 4594
Fragments arranged by size:
4594 2744 685
Acc65I G'GTAC_C
Cuts at: 5344 5344
Size: 8023
AclI AA'CG_TT
Cuts at: 1563 6150 6523 1563
Size: 4587 373 3063
Fragments arranged by size:
4587 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3980 4405 6086 7470 7738 1632
Size: 2348 425 1681 1384 268 1917
Fragments arranged by size:
2348 1917 1681 1384 425 268
AfeI AGC'GCT
Cuts at: 3088 3088
Size: 8023
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7826
AflIII A'CryG_T
Cuts at: 1203 3117 4659 6922 7899 1203
Size: 1914 1542 2263 977 1327
Fragments arranged by size:
2263 1914 1542 1327 977
AgeI A'CCGG_T
Cuts at: 6854 6854
Size: 8023
AhdI GACnn_n'nnGTC
Cuts at: 4303 4663 6752 4303
Size: 360 2089 5574
Fragments arranged by size:
5574 2089 360
AleI CACnn'nnGTG
Cuts at: 1166 2532 1166
Size: 1366 6657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4178 4613 1184
Size: 2994 435 4594
Fragments arranged by size:
4594 2994 435
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3288 3320 3288
Size: 32 7991
AlwNI CAG_nnn'CTG
Cuts at: 2101 3176 5766 7338 2101
Size: 1075 2590 1572 2786
Fragments arranged by size:
2786 2590 1572 1075
ApaI G_GGCC'C
Cuts at: 3984 5114 3984
Size: 1130 6893
ApaLI G'TGCA_C
Cuts at: 6080 7236 6080
Size: 1156 6867
ApoI r'AATT_y
Cuts at: 1493 5332 5558 5710 7819 1493
Size: 3839 226 152 2109 1697
Fragments arranged by size:
3839 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6577 160
Size: 872 5545 1606
Fragments arranged by size:
5545 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8023
AvaI C'yCGr_G
Cuts at: 1870 1943 2116 2167 2860 4548 4877 5093 5295
Size: 73 173 51 693 1688 329 216 202
Cuts at: 5295 5348 1870
Size: 53 4545
Fragments arranged by size:
4545 1688 693 329 216 202 173 73
53 51
BamHI G'GATC_C
Cuts at: 4674 5353 5720 4674
Size: 679 367 6977
Fragments arranged by size:
6977 679 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2240 3574 4706 5283 5344
Size: 325 217 627 453 1334 1132 577 61
Cuts at: 5344 6799 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1334 1132 627 577 453 325
217 61
BanII G_rGCy'C
Cuts at: 1391 2211 3725 3984 4149 4176 5114 5202 5226
Size: 820 1514 259 165 27 938 88 24
Cuts at: 5226 5238 5342 1391
Size: 12 104 4072
Fragments arranged by size:
4072 1514 938 820 259 165 104 88
27 24 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 928
Size: 971 963 6089
Fragments arranged by size:
6089 971 963
BbvCI CC'TCA_GC
Cuts at: 3213 3213
Size: 8023
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2698 2732 3488 3522 6214
Size: 34 50 34 677 34 756 34 2692
Cuts at: 6214 6248 1903
Size: 34 3678
Fragments arranged by size:
3678 2692 756 677 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5915 7125 5915
Size: 1210 6813
BclI T'GATC_A
Cuts at: 1548 2524 4377 1548
Size: 976 1853 5194
Fragments arranged by size:
5194 1853 976
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3620 4171 6634
Size: 107 122 71 631 605 1948 551 2463
Cuts at: 6634 136
Size: 1525
Fragments arranged by size:
2463 1948 1525 631 605 551 122 107
71
BglII A'GATC_T
Cuts at: 2088 4527 4689 4800 8018 2088
Size: 2439 162 111 3218 2093
Fragments arranged by size:
3218 2439 2093 162 111
BlpI GC'TnA_GC
Cuts at: 2988 4397 5440 2988
Size: 1409 1043 5571
Fragments arranged by size:
5571 1409 1043
Bme1580I G_kGCm'C
Cuts at: 1790 2975 3984 4263 5114 5288 6084 7240 1790
Size: 1185 1009 279 851 174 796 1156 2573
Fragments arranged by size:
2573 1185 1156 1009 851 796 279 174
BmgBI CAC'GTC
Cuts at: 1318 4660 1318
Size: 3342 4681
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2286 2835 3460
Size: 416 53 83 399 24 869 549 625
Cuts at: 3460 5754 6712 442
Size: 2294 958 1753
Fragments arranged by size:
2294 1753 958 869 625 549 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8023
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3894 3894
Size: 8023
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2128 2233 2348 2615 3067 3284 3452 4374
Size: 889 105 115 267 452 217 168 922
Cuts at: 4374 5138 5216 5222 6683 1239
Size: 764 78 6 1461 2579
Fragments arranged by size:
2579 1461 922 889 764 452 267 217
168 115 105 78 6
Bpu10I CC'TnA_GC
Cuts at: 3213 3869 4652 3213
Size: 656 783 6584
Fragments arranged by size:
6584 783 656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5252 6148 7013 7311 5252
Size: 896 865 298 5964
Fragments arranged by size:
5964 896 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2324 3119 3354 4059 4473 6686 882
Size: 617 825 795 235 705 414 2213 2219
Fragments arranged by size:
2219 2213 825 795 705 617 414 235
BsaAI yAC'GTr
Cuts at: 493 1204 2950 3380 3502 3748 3780 5814 493
Size: 711 1746 430 122 246 32 2034 2702
Fragments arranged by size:
2702 2034 1746 711 430 246 122 32
BsaBI GATnn'nnATC
Cuts at: 8017 8017
Size: 8023
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4284 5830 6212 6847
Size: 53 83 186 936 2751 1546 382 635
Cuts at: 6847 275
Size: 1451
Fragments arranged by size:
2751 1546 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2179 4618 6456 6854 7128 7275 7686 2179
Size: 2439 1838 398 274 147 411 2516
Fragments arranged by size:
2516 2439 1838 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 2807 2837 3116
Size: 30 165 30 897 30 477 30 279
Cuts at: 3116 3146 3452 3482 4056 4086 5003 5033 1178
Size: 30 306 30 574 30 917 30 4168
Fragments arranged by size:
4168 917 897 574 477 306 279 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3434 3470 3473 4943 4954 5158 1186
Size: 2248 36 3 1470 11 204 4051
Fragments arranged by size:
4051 2248 1470 204 36 11 3
BseYI C'CCAG_C
Cuts at: 3330 3400 3808 4136 4184 4776 4824 4860 4951
Size: 70 408 328 48 592 48 36 91
Cuts at: 4951 5129 5226 7226 7722 3330
Size: 178 97 2000 496 3631
Fragments arranged by size:
3631 2000 592 496 408 328 178 97
91 70 48 48 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2468 2687 2816 3511 4000 4067 5060
Size: 573 732 219 129 695 489 67 993
Cuts at: 5060 1163
Size: 4126
Fragments arranged by size:
4126 993 732 695 573 489 219 129
67
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5473 6234 7262 1540
Size: 275 186 3472 761 1028 2301
Fragments arranged by size:
3472 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 3485 3636 3725 4149 4503 5238 6084 6169
Size: 1226 151 89 424 354 735 846 85
Cuts at: 6169 7240 2259
Size: 1071 3042
Fragments arranged by size:
3042 1226 1071 846 735 424 354 151
89 85
BsmI GAATG_Cn'
Cuts at: 5534 5627 5534
Size: 93 7930
BsmBI CGTCTCn'nnnn_
Cuts at: 2656 4275 2656
Size: 1619 6404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2259 2975 3485 3636 3725 3984
Size: 399 421 48 716 510 151 89 259
Cuts at: 3984 4149 4176 4263 4503 5114 5202 5226 5238
Size: 165 27 87 240 611 88 24 12
Cuts at: 5238 5288 5342 6084 6169 7240 1391
Size: 50 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 716 611 510 421 399
259 240 165 151 89 88 87 85
54 50 48 27 24 12
BspEI T'CCGG_A
Cuts at: 4618 7686 4618
Size: 3068 4955
BspHI T'CATG_A
Cuts at: 3966 5912 7642 3966
Size: 1946 1730 4347
Fragments arranged by size:
4347 1946 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2171 2882 3004 3832 4089 4370 4909 5378
Size: 1327 711 122 828 257 281 539 469
Cuts at: 5378 844
Size: 3489
Fragments arranged by size:
3489 1327 828 711 539 469 281 257
122
BsrBI CCG'CTC
Cuts at: 1394 1823 2341 2823 2943 5910 1394
Size: 429 518 482 120 2967 3507
Fragments arranged by size:
3507 2967 518 482 429 120
BsrDI GCAATG_nn'
Cuts at: 65 1248 2884 4162 6518 6692 65
Size: 1183 1636 1278 2356 174 1396
Fragments arranged by size:
2356 1636 1396 1278 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2782 6667 6854 1700
Size: 110 972 3885 187 2869
Fragments arranged by size:
3885 2869 972 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8023
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8023
BssSI C'ACGA_G
Cuts at: 2486 4412 6083 7095 2486
Size: 1926 1671 1012 3414
Fragments arranged by size:
3414 1926 1671 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8023
BstBI TT'CG_AA
Cuts at: 6827 6827
Size: 8023
BstEII G'GTnAC_C
Cuts at: 1184 4763 1184
Size: 3579 4444
BstXI CCAn_nnnn'nTGG
Cuts at: 3400 4318 4444 3400
Size: 918 126 6979
Fragments arranged by size:
6979 918 126
BstYI r'GATC_y
Cuts at: 1972 2088 2368 3693 4527 4674 4689 4800 5353
Size: 116 280 1325 834 147 15 111 553
Cuts at: 5353 5720 6105 6122 7574 7660 7672 8018 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1325 834 553 385 367 346
280 147 116 111 86 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2320 2376 2931 3791 3855 4990 513
Size: 552 1255 56 555 860 64 1135 3546
Fragments arranged by size:
3546 1255 1135 860 555 552 64 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2036 487
Size: 683 56 810 6474
Fragments arranged by size:
6474 810 683 56
BtsI GCAGTG_nn'
Cuts at: 2480 2507 2670 3008 3485 3937 5539 6333 6353
Size: 27 163 338 477 452 1602 794 20
Cuts at: 6353 2480
Size: 4150
Fragments arranged by size:
4150 1602 794 477 452 338 163 27
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8023
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2746 2781 3567 3602 529
Size: 35 2182 35 786 35 4950
Fragments arranged by size:
4950 2182 786 35 35 35
DraI TTT'AAA
Cuts at: 5327 5327
Size: 8023
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7030 809
Size: 507 5714 1802
Fragments arranged by size:
5714 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 3178 3316
Size: 54 1425 50 275 18 393 955 138
Cuts at: 3316 3509 3730 3897 4434 5470 6359 7988 8
Size: 193 221 167 537 1036 889 1629 43
Fragments arranged by size:
1629 1425 1036 955 889 537 393 275
221 193 167 138 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5470 1537
Size: 275 3658 4090
Fragments arranged by size:
4090 3658 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3411 4824 5953 1353
Size: 2058 1413 1129 3423
Fragments arranged by size:
3423 2058 1413 1129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2669 5315 6610 6984 7130 387
Size: 1064 138 1080 2646 1295 374 146 1280
Fragments arranged by size:
2646 1295 1280 1080 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3723 4147 5236 3723
Size: 424 1089 6510
Fragments arranged by size:
6510 1089 424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 2233 2348 2615 3067 3284 3452
Size: 393 496 105 115 267 452 217 168
Cuts at: 3452 3980 4374 4405 5138 5216 5222 6086 6683
Size: 528 394 31 733 78 6 864 597
Cuts at: 6683 7470 7738 1239
Size: 787 268 1524
Fragments arranged by size:
1524 864 787 733 597 528 496 452
394 393 268 267 217 168 115 105
78 31 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2963 2963
Size: 8023
EcoO109I rG'GnC_Cy
Cuts at: 2098 2865 3831 3981 4011 4028 4359 5110 2098
Size: 767 966 150 30 17 331 751 5011
Fragments arranged by size:
5011 966 767 751 331 150 30 17
EcoRI G'AATT_C
Cuts at: 5332 5710 5332
Size: 378 7645
FspI TGC'GCA
Cuts at: 3536 6529 3536
Size: 2993 5030
HaeII r_GCGC'y
Cuts at: 1678 3090 4129 7170 1678
Size: 1412 1039 3041 2531
Fragments arranged by size:
3041 2531 1412 1039
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2014 2046 3804 3836 3955
Size: 32 135 32 442 32 1758 32 119
Cuts at: 3955 3987 4017 4049 4289 4321 4649 4681 5003
Size: 32 30 32 240 32 328 32 322
Cuts at: 5003 5035 6664 6696 6738 6770 1373
Size: 32 1629 32 42 32 2626
Fragments arranged by size:
2626 1758 1629 442 328 322 240 135
119 42 32 32 32 32 32 32
32 32 32 32 32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 4000 5161 5367 5613 669
Size: 988 281 796 1266 1161 206 246 3079
Fragments arranged by size:
3079 1266 1161 988 796 281 246 206
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8023
HpaI GTT'AAC
Cuts at: 5613 5613
Size: 8023
KpnI G_GTAC'C
Cuts at: 5348 5348
Size: 8023
MfeI C'AATT_G
Cuts at: 5622 5622
Size: 8023
MluI A'CGCG_T
Cuts at: 7899 7899
Size: 8023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3102 3933 4143 4335 4818 7137 7321 1305
Size: 1797 831 210 192 483 2319 184 2007
Fragments arranged by size:
2319 2007 1797 831 483 210 192 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2225 3180 4436 10
Size: 54 1425 343 393 955 1256 3597
Fragments arranged by size:
3597 1425 1256 955 393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2532 2702 2907 2930 3207 3572
Size: 648 1194 172 170 205 23 277 365
Cuts at: 3572 3746 4229 5981 6340 6499 518
Size: 174 483 1752 359 159 2042
Fragments arranged by size:
2042 1752 1194 648 483 365 359 277
205 174 172 170 159 23
MspA1I CmG'CkG
Cuts at: 1606 2470 3309 3430 3763 3793 4864 5439 6116
Size: 864 839 121 333 30 1071 575 677
Cuts at: 6116 7264 7509 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1071 864 839 677 575 333
245 121 30
NcoI C'CATG_G
Cuts at: 513 1065 4990 513
Size: 552 3925 3546
Fragments arranged by size:
3925 3546 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8023
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8023
NotI GC'GGCC_GC
Cuts at: 5470 5470
Size: 8023
NspI r_CATG'y
Cuts at: 1132 1229 3328 5381 6926 1132
Size: 97 2099 2053 1545 2229
Fragments arranged by size:
2229 2099 2053 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3073 1348
Size: 1725 6298
PciI A'CATG_T
Cuts at: 6922 6922
Size: 8023
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4921 1108
Size: 3813 4210
PmeI GTTT'AAAC
Cuts at: 5327 5327
Size: 8023
PmlI CAC'GTG
Cuts at: 3380 3502 3780 3380
Size: 122 278 7623
Fragments arranged by size:
7623 278 122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6092 6124 7605 7637 6092
Size: 32 1481 32 6478
Fragments arranged by size:
6478 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2865 4028 4359 2865
Size: 1163 331 6529
Fragments arranged by size:
6529 1163 331
PshAI GACnn'nnGTC
Cuts at: 1613 2675 1613
Size: 1062 6961
PsiI TTA'TAA
Cuts at: 5593 5593
Size: 8023
PspOMI G'GGCC_C
Cuts at: 3980 5110 3980
Size: 1130 6893
PstI C_TGCA'G
Cuts at: 830 3015 3359 3498 4110 5375 830
Size: 2185 344 139 612 1265 3478
Fragments arranged by size:
3478 2185 1265 612 344 139
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8023
PvuII CAG'CTG
Cuts at: 2470 3430 3763 4864 2470
Size: 960 333 1101 5629
Fragments arranged by size:
5629 1101 960 333
SacI G_AGCT'C
Cuts at: 3725 4149 5238 3725
Size: 424 1089 6510
Fragments arranged by size:
6510 1089 424
SacII CC_GC'GG
Cuts at: 3794 3794
Size: 8023
SalI G'TCGA_C
Cuts at: 1936 5365 1936
Size: 3429 4594
SbfI CC_TGCA'GG
Cuts at: 5375 5375
Size: 8023
ScaI AGT'ACT
Cuts at: 4052 6271 7983 4052
Size: 2219 1712 4092
Fragments arranged by size:
4092 2219 1712
SexAI A'CCwGG_T
Cuts at: 4068 4428 4068
Size: 360 7663
SfcI C'TryA_G
Cuts at: 826 1045 2623 3011 3236 3355 3494 4106 4150
Size: 219 1578 388 225 119 139 612 44
Cuts at: 4150 5371 6506 7187 7378 826
Size: 1221 1135 681 191 1471
Fragments arranged by size:
1578 1471 1221 1135 681 612 388 225
219 191 139 119 44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8023
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8023
SmaI CCC'GGG
Cuts at: 1872 2118 5350 1872
Size: 246 3232 4545
Fragments arranged by size:
4545 3232 246
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4548 4877 5231 6127 7028 7290
Size: 197 926 2605 329 354 896 901 262
Cuts at: 7290 820
Size: 1553
Fragments arranged by size:
2605 1553 926 901 896 354 329 262
197
SnaBI TAC'GTA
Cuts at: 493 5814 493
Size: 5321 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8023
SphI G_CATG'C
Cuts at: 1132 3328 5381 1132
Size: 2196 2053 3774
Fragments arranged by size:
3774 2196 2053
SspI AAT'ATT
Cuts at: 5 52 5947 5
Size: 47 5895 2081
Fragments arranged by size:
5895 2081 47
StuI AGG'CCT
Cuts at: 1517 3029 1517
Size: 1512 6511
StyI C'CwwG_G
Cuts at: 513 1065 2226 3976 4698 4990 5462 513
Size: 552 1161 1750 722 292 472 3074
Fragments arranged by size:
3074 1750 1161 722 552 472 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2321 2348 4691 4953 4965 6050 6067
Size: 1255 741 27 2343 262 12 1085 17
Cuts at: 6067 6220 6405 6850 325
Size: 153 185 445 1498
Fragments arranged by size:
2343 1498 1255 1085 741 445 262 185
153 27 17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3738 4050 6269
Size: 274 80 33 51 1443 1761 312 2219
Cuts at: 6269 7981 96
Size: 1712 138
Fragments arranged by size:
2219 1761 1712 1443 312 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2300 2332 2499 2531 2807 2839 4056 4088 5267
Size: 32 167 32 276 32 1217 32 1179
Cuts at: 5267 5299 7983 8015 2300
Size: 32 2684 32 2308
Fragments arranged by size:
2684 2308 1217 1179 276 167 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8023
XbaI T'CTAG_A
Cuts at: 5359 5359
Size: 8023
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3287 3622 3892 4436 3287
Size: 335 270 544 6874
Fragments arranged by size:
6874 544 335 270
XhoI C'TCGA_G
Cuts at: 1943 4548 4877 1943
Size: 2605 329 5089
Fragments arranged by size:
5089 2605 329
XmaI C'CCGG_G
Cuts at: 1870 2116 5348 1870
Size: 246 3232 4545
Fragments arranged by size:
4545 3232 246
XmnI GAAnn'nnTTC
Cuts at: 6152 6152
Size: 8023
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4285 5831 276
Size: 53 83 186 936 2751 1546 2468
Fragments arranged by size:
2751 2468 1546 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII
SanDI SapI SfiI SfoI SrfI SwaI