Restriction map for
pFN21AB7022
(Circular) (Six-base) MAPSORT of: pFN21AB7022.seq Check: 1445 from: 1 to: 7621
pFN21AB7022 7621 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4518 5431 278
Size: 53 83 186 936 2982 913 2468
Fragments arranged by size:
2982 2468 936 913 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4095 4497 4964 1937
Size: 2158 402 467 4594
Fragments arranged by size:
4594 2158 467 402
Acc65I G'GTAC_C
Cuts at: 3273 4942 3273
Size: 1669 5952
AclI AA'CG_TT
Cuts at: 1563 5748 6121 1563
Size: 4185 373 3063
Fragments arranged by size:
4185 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3137 3857 3878 3926 4718 5684 7068 7336
Size: 1505 720 21 48 792 966 1384 268
Cuts at: 7336 1632
Size: 1917
Fragments arranged by size:
1917 1505 1384 966 792 720 268 48
21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7424
AflIII A'CryG_T
Cuts at: 1203 6520 7497 1203
Size: 5317 977 1327
Fragments arranged by size:
5317 1327 977
AgeI A'CCGG_T
Cuts at: 6452 6452
Size: 7621
AhdI GACnn_n'nnGTC
Cuts at: 4368 4519 6350 4368
Size: 151 1831 5639
Fragments arranged by size:
5639 1831 151
AleI CACnn'nnGTG
Cuts at: 1166 3554 4172 1166
Size: 2388 618 4615
Fragments arranged by size:
4615 2388 618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7621
AlwNI CAG_nnn'CTG
Cuts at: 3415 5364 6936 3415
Size: 1949 1572 4100
Fragments arranged by size:
4100 1949 1572
ApaI G_GGCC'C
Cuts at: 2012 2610 3466 2012
Size: 598 856 6167
Fragments arranged by size:
6167 856 598
ApaLI G'TGCA_C
Cuts at: 5678 6834 5678
Size: 1156 6465
ApoI r'AATT_y
Cuts at: 1493 4930 5156 5308 7417 1493
Size: 3437 226 152 2109 1697
Fragments arranged by size:
3437 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6175 160
Size: 872 5143 1606
Fragments arranged by size:
5143 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7621
AvaI C'yCGr_G
Cuts at: 1870 1943 4082 4115 4946 1870
Size: 73 2139 33 831 4545
Fragments arranged by size:
4545 2139 831 73 33
BamHI G'GATC_C
Cuts at: 4951 5318 4951
Size: 367 7254
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2626 3248 3273 4942 6397
Size: 325 217 627 839 622 25 1669 1455
Cuts at: 6397 618
Size: 1842
Fragments arranged by size:
1842 1669 1455 839 627 622 325 217
25
BanII G_rGCy'C
Cuts at: 1391 2012 2079 2610 2918 2961 3051 3178 3192
Size: 621 67 531 308 43 90 127 14
Cuts at: 3192 3390 3466 3520 4070 4260 4695 4844 4940
Size: 198 76 54 550 190 435 149 96
Cuts at: 4940 1391
Size: 4072
Fragments arranged by size:
4072 621 550 531 435 308 198 190
149 127 96 90 76 67 54 43
14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2701 928
Size: 971 802 5848
Fragments arranged by size:
5848 971 802
BbvCI CC'TCA_GC
Cuts at: 2210 4041 2210
Size: 1831 5790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2400 2434 5812 5846 1903
Size: 34 463 34 3378 34 3678
Fragments arranged by size:
3678 3378 463 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4613 5513 6723 4613
Size: 900 1210 5511
Fragments arranged by size:
5511 1210 900
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7621
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3924 4682 6232
Size: 107 122 71 631 605 2252 758 1550
Cuts at: 6232 136
Size: 1525
Fragments arranged by size:
2252 1550 1525 758 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4545 4719 7616 4545
Size: 174 2897 4550
Fragments arranged by size:
4550 2897 174
BlpI GC'TnA_GC
Cuts at: 2677 5038 2677
Size: 2361 5260
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2542 2610 3466 5682 6838 1790
Size: 222 530 68 856 2216 1156 2573
Fragments arranged by size:
2573 2216 1156 856 530 222 68
BmgBI CAC'GTC
Cuts at: 1318 4339 1318
Size: 3021 4600
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2060 2073 5352
Size: 416 53 83 399 24 643 13 3279
Cuts at: 5352 6310 442
Size: 958 1753
Fragments arranged by size:
3279 1753 958 643 416 399 83 53
24 13
BmtI G_CTAG'C
Cuts at: 1056 2339 1056
Size: 1283 6338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4567 4858 4567
Size: 291 7330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2182 2286 3190 3795 4135 4264 4274 4850
Size: 943 104 904 605 340 129 10 576
Cuts at: 4850 6281 1239
Size: 1431 2579
Fragments arranged by size:
2579 1431 943 904 605 576 340 129
104 10
Bpu10I CC'TnA_GC
Cuts at: 2088 2210 3045 3377 4041 4064 2088
Size: 122 835 332 664 23 5645
Fragments arranged by size:
5645 835 664 332 122 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2932 5746 6611 6909 2932
Size: 2814 865 298 3644
Fragments arranged by size:
3644 2814 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2262 3422 6284 882
Size: 617 763 1160 2862 2219
Fragments arranged by size:
2862 2219 1160 763 617
BsaAI yAC'GTr
Cuts at: 493 1204 5412 493
Size: 711 4208 2702
Fragments arranged by size:
4208 2702 711
BsaBI GATnn'nnATC
Cuts at: 2567 7615 2567
Size: 5048 2573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2415 4092 4515 5428
Size: 53 83 186 936 882 1677 423 913
Cuts at: 5428 5810 6445 275
Size: 382 635 1451
Fragments arranged by size:
1677 1451 936 913 882 635 423 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 6054 6452 6726 6873 7284 6054
Size: 398 274 147 411 6391
Fragments arranged by size:
6391 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2100 2130 2855 2885 3963
Size: 30 165 30 697 30 725 30 1078
Cuts at: 3963 3993 1178
Size: 30 4806
Fragments arranged by size:
4806 1078 725 697 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4526 4847 4850 1186
Size: 3340 321 3 3957
Fragments arranged by size:
3957 3340 321 3
BseYI C'CCAG_C
Cuts at: 2611 3279 4051 4058 4632 4680 4884 6824 7320
Size: 668 772 7 574 48 204 1940 496
Cuts at: 7320 2611
Size: 2912
Fragments arranged by size:
2912 1940 772 668 574 496 204 48
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2239 2609 2873 3325 3428 3478 1163
Size: 573 503 370 264 452 103 50 5306
Fragments arranged by size:
5306 573 503 452 370 264 103 50
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5071 5832 6860 1540
Size: 275 186 3070 761 1028 2301
Fragments arranged by size:
3070 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2678 2918 3178 3192 3441 4260 4844 4854 5682
Size: 240 260 14 249 819 584 10 828
Cuts at: 5682 5767 6838 2678
Size: 85 1071 3461
Fragments arranged by size:
3461 1071 828 819 584 260 249 240
85 14 10
BsmI GAATG_Cn'
Cuts at: 5132 5225 5132
Size: 93 7528
BsmBI CGTCTCn'nnnn_
Cuts at: 2422 2422
Size: 7621
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2079 2542 2610 2678 2918 2961
Size: 399 222 67 463 68 68 240 43
Cuts at: 2961 3051 3178 3192 3390 3441 3466 3520 4070
Size: 90 127 14 198 51 25 54 550
Cuts at: 4070 4260 4695 4844 4854 4940 5682 5767 6838
Size: 190 435 149 10 86 742 85 1071
Cuts at: 6838 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 550 463 435 399 240
222 198 190 149 127 90 86 85
68 68 67 54 51 43 25 14
10
BspEI T'CCGG_A
Cuts at: 7284 7284
Size: 7621
BspHI T'CATG_A
Cuts at: 3912 4413 5510 7240 3912
Size: 501 1097 1730 4293
Fragments arranged by size:
4293 1730 1097 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3337 3756 4023 4275 4868 4976 844
Size: 2493 419 267 252 593 108 3489
Fragments arranged by size:
3489 2493 593 419 267 252 108
BsrBI CCG'CTC
Cuts at: 1394 1823 4765 5508 1394
Size: 429 2942 743 3507
Fragments arranged by size:
3507 2942 743 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2143 4450 6116 6290 65
Size: 1183 895 2307 1666 174 1396
Fragments arranged by size:
2307 1666 1396 1183 895 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2439 2822 6265 6452 1700
Size: 110 629 383 3443 187 2869
Fragments arranged by size:
3443 2869 629 383 187 110
BsrGI T'GTAC_A
Cuts at: 96 3615 96
Size: 3519 4102
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7621
BssSI C'ACGA_G
Cuts at: 3932 5681 6693 3932
Size: 1749 1012 4860
Fragments arranged by size:
4860 1749 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3415 794
Size: 2621 5000
BstBI TT'CG_AA
Cuts at: 6425 6425
Size: 7621
BstEII G'GTnAC_C
Cuts at: 1184 2116 2941 1184
Size: 932 825 5864
Fragments arranged by size:
5864 932 825
BstXI CCAn_nnnn'nTGG
Cuts at: 3168 3168
Size: 7621
BstYI r'GATC_y
Cuts at: 1972 4545 4719 4951 5318 5703 5720 7172 7258
Size: 2573 174 232 367 385 17 1452 86
Cuts at: 7258 7270 7616 1972
Size: 12 346 1977
Fragments arranged by size:
2573 1977 1452 385 367 346 232 174
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3369 3369
Size: 7621
BtgI C'CryG_G
Cuts at: 513 1065 2004 2946 3801 3864 3967 513
Size: 552 939 942 855 63 103 4167
Fragments arranged by size:
4167 942 939 855 552 103 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2140 487
Size: 683 56 914 5968
Fragments arranged by size:
5968 914 683 56
BtsI GCAGTG_nn'
Cuts at: 2542 3308 3313 4828 5137 5931 5951 2542
Size: 766 5 1515 309 794 20 4212
Fragments arranged by size:
4212 1515 794 766 309 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7621
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3790 3825 529
Size: 35 3226 35 4325
Fragments arranged by size:
4325 3226 35 35
DraI TTT'AAA
Cuts at: 4925 4925
Size: 7621
DraIII CAC_nnn'GTG
Cuts at: 3400 3400
Size: 7621
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6628 809
Size: 507 5312 1802
Fragments arranged by size:
5312 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2437 2841
Size: 54 1425 50 275 18 207 400 404
Cuts at: 2841 3133 3150 3310 4072 4377 5068 5957 7586
Size: 292 17 160 762 305 691 889 1629
Cuts at: 7586 8
Size: 43
Fragments arranged by size:
1629 1425 889 762 691 404 400 305
292 275 207 160 54 50 43 18
17
EagI C'GGCC_G
Cuts at: 1537 1812 5068 1537
Size: 275 3256 4090
Fragments arranged by size:
4090 3256 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3551 5551 1353
Size: 2198 2000 3423
Fragments arranged by size:
3423 2198 2000
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6208 6582 6728 387
Size: 1064 138 4619 374 146 1280
Fragments arranged by size:
4619 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2916 3176 3190 4258 4842 2916
Size: 260 14 1068 584 5695
Fragments arranged by size:
5695 1068 584 260 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2286 3137 3190 3795 3857 3878
Size: 393 550 104 851 53 605 62 21
Cuts at: 3878 3926 4135 4264 4274 4718 4850 5684 6281
Size: 48 209 129 10 444 132 834 597
Cuts at: 6281 7068 7336 1239
Size: 787 268 1524
Fragments arranged by size:
1524 851 834 787 605 597 550 444
393 268 209 132 129 104 62 53
48 21 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2933 3380 4232 2933
Size: 447 852 6322
Fragments arranged by size:
6322 852 447
EcoO109I rG'GnC_Cy
Cuts at: 2244 2606 2607 3023 3373 3462 3889 3974 4055
Size: 362 1 416 350 89 427 85 81
Cuts at: 4055 4078 4264 2244
Size: 23 186 5601
Fragments arranged by size:
5601 427 416 362 350 186 89 85
81 23 1
EcoRI G'AATT_C
Cuts at: 4930 5308 4930
Size: 378 7243
EcoRV GAT'ATC
Cuts at: 4879 4879
Size: 7621
FseI GG_CCGG'CC
Cuts at: 2443 2443
Size: 7621
FspI TGC'GCA
Cuts at: 6127 6127
Size: 7621
HaeII r_GCGC'y
Cuts at: 1678 2289 6768 1678
Size: 611 4479 2531
Fragments arranged by size:
4479 2531 611
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4199 4231 4354 4386 4505
Size: 32 135 32 2627 32 123 32 119
Cuts at: 4505 4537 6262 6294 6336 6368 1373
Size: 32 1725 32 42 32 2626
Fragments arranged by size:
2627 2626 1725 135 123 119 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2698 3946 4096 4362 4965 5211
Size: 988 281 760 1248 150 266 603 246
Cuts at: 5211 669
Size: 3079
Fragments arranged by size:
3079 1248 988 760 603 281 266 246
150
HindIII A'AGCT_T
Cuts at: 748 3053 748
Size: 2305 5316
HpaI GTT'AAC
Cuts at: 2698 5211 2698
Size: 2513 5108
KpnI G_GTAC'C
Cuts at: 3277 4946 3277
Size: 1669 5952
MfeI C'AATT_G
Cuts at: 5220 5220
Size: 7621
MluI A'CGCG_T
Cuts at: 7497 7497
Size: 7621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2740 2839 4449 6735 6919 1305
Size: 1435 99 1610 2286 184 2007
Fragments arranged by size:
2286 2007 1610 1435 184 99
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 2843 3152 3312 4379
Size: 54 1425 343 207 804 309 160 1067
Cuts at: 4379 10
Size: 3252
Fragments arranged by size:
3252 1425 1067 804 343 309 207 160
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 2873 3554 3876 4172 4583 5579
Size: 648 1135 572 681 322 296 411 996
Cuts at: 5579 5938 6097 518
Size: 359 159 2042
Fragments arranged by size:
2042 1135 996 681 648 572 411 359
322 296 159
MspA1I CmG'CkG
Cuts at: 1606 3651 3709 4809 5037 5714 6862 7107 1606
Size: 2045 58 1100 228 677 1148 245 2120
Fragments arranged by size:
2120 2045 1148 1100 677 245 228 58
NaeI GCC'GGC
Cuts at: 2441 2824 2441
Size: 383 7238
NcoI C'CATG_G
Cuts at: 513 1065 2004 3967 513
Size: 552 939 1963 4167
Fragments arranged by size:
4167 1963 939 552
NdeI CA'TA_TG
Cuts at: 387 3224 387
Size: 2837 4784
NgoMIV G'CCGG_C
Cuts at: 2439 2822 2439
Size: 383 7238
NheI G'CTAG_C
Cuts at: 1052 2335 1052
Size: 1283 6338
NotI GC'GGCC_GC
Cuts at: 5068 5068
Size: 7621
NspI r_CATG'y
Cuts at: 1132 1229 2822 3574 4979 6524 1132
Size: 97 1593 752 1405 1545 2229
Fragments arranged by size:
2229 1593 1545 1405 752 97
PasI CC'CwG_GG
Cuts at: 1348 2275 2603 4294 1348
Size: 927 328 1691 4675
Fragments arranged by size:
4675 1691 927 328
PciI A'CATG_T
Cuts at: 6520 6520
Size: 7621
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2046 4162 4300 1108
Size: 938 2116 138 4429
Fragments arranged by size:
4429 2116 938 138
PfoI T'CCnGG_A
Cuts at: 2934 4260 2934
Size: 1326 6295
PmeI GTTT'AAAC
Cuts at: 4925 4925
Size: 7621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 5690 5722 7203 7235 2900
Size: 32 2758 32 1481 32 3286
Fragments arranged by size:
3286 2758 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2244 3023 3373 4055 4078 4264 2244
Size: 779 350 682 23 186 5601
Fragments arranged by size:
5601 779 682 350 186 23
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7621
PsiI TTA'TAA
Cuts at: 5191 5191
Size: 7621
PspOMI G'GGCC_C
Cuts at: 2008 2606 3462 2008
Size: 598 856 6167
Fragments arranged by size:
6167 856 598
PstI C_TGCA'G
Cuts at: 830 2635 3303 3348 3514 3753 4899 4973 830
Size: 1805 668 45 166 239 1146 74 3478
Fragments arranged by size:
3478 1805 1146 668 239 166 74 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7621
PvuII CAG'CTG
Cuts at: 3709 3709
Size: 7621
SacI G_AGCT'C
Cuts at: 2918 3178 3192 4260 4844 2918
Size: 260 14 1068 584 5695
Fragments arranged by size:
5695 1068 584 260 14
SalI G'TCGA_C
Cuts at: 1936 4094 4963 1936
Size: 2158 869 4594
Fragments arranged by size:
4594 2158 869
SanDI GG'GwC_CC
Cuts at: 3023 4055 3023
Size: 1032 6589
SbfI CC_TGCA'GG
Cuts at: 4973 4973
Size: 7621
ScaI AGT'ACT
Cuts at: 3998 5869 7581 3998
Size: 1871 1712 4038
Fragments arranged by size:
4038 1871 1712
SexAI A'CCwGG_T
Cuts at: 3642 3642
Size: 7621
SfcI C'TryA_G
Cuts at: 826 1045 2631 3299 3344 3510 3749 4895 4969
Size: 219 1586 668 45 166 239 1146 74
Cuts at: 4969 6104 6785 6976 826
Size: 1135 681 191 1471
Fragments arranged by size:
1586 1471 1146 1135 681 668 239 219
191 166 74 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 4682 4682
Size: 7621
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7621
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7621
SmaI CCC'GGG
Cuts at: 1872 4948 1872
Size: 3076 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2911 4115 5725 6626 6888 820
Size: 197 926 968 1204 1610 901 262 1553
Fragments arranged by size:
1610 1553 1204 968 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5412 493
Size: 4919 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7621
SphI G_CATG'C
Cuts at: 1132 4979 1132
Size: 3847 3774
SspI AAT'ATT
Cuts at: 5 52 5545 5
Size: 47 5493 2081
Fragments arranged by size:
5493 2081 47
StuI AGG'CCT
Cuts at: 1517 2984 2994 4186 4676 1517
Size: 1467 10 1192 490 4462
Fragments arranged by size:
4462 1467 1192 490 10
StyI C'CwwG_G
Cuts at: 513 1065 2004 3072 3967 4686 5060 513
Size: 552 939 1068 895 719 374 3074
Fragments arranged by size:
3074 1068 939 895 719 552 374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2537 4712 5648 5665 5818 6003 6448
Size: 1255 957 2175 936 17 153 185 445
Cuts at: 6448 325
Size: 1498
Fragments arranged by size:
2175 1498 1255 957 936 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 3615 3979
Size: 274 80 33 51 1443 207 1431 364
Cuts at: 3979 3996 5867 7579 96
Size: 17 1871 1712 138
Fragments arranged by size:
1871 1712 1443 1431 364 274 207 138
80 51 33 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2052 2084 2100 2132 2855 2887 7581 7613 2052
Size: 32 16 32 723 32 4694 32 2060
Fragments arranged by size:
4694 2060 723 32 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 4493 1316
Size: 3177 4444
XbaI T'CTAG_A
Cuts at: 4957 4957
Size: 7621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2172 4159 4372 2172
Size: 1987 213 5421
Fragments arranged by size:
5421 1987 213
XhoI C'TCGA_G
Cuts at: 1943 4115 1943
Size: 2172 5449
XmaI C'CCGG_G
Cuts at: 1870 4946 1870
Size: 3076 4545
XmnI GAAnn'nnTTC
Cuts at: 5750 5750
Size: 7621
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4516 5429 276
Size: 53 83 186 936 2982 913 2468
Fragments arranged by size:
2982 2468 936 913 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I FalI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SapI SfoI
SrfI SwaI