Restriction map for
pFN21AB7018
(Circular) (Six-base) MAPSORT of: pFN21AB7018.seq Check: 8336 from: 1 to: 7621
pFN21AB7018 7621 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4687 4687
Size: 7621
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3201 4548 5431 278
Size: 53 83 186 936 1665 1347 883 2468
Fragments arranged by size:
2468 1665 1347 936 883 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4527 4845 4964 1937
Size: 2590 318 119 4594
Fragments arranged by size:
4594 2590 318 119
Acc65I G'GTAC_C
Cuts at: 4942 4942
Size: 7621
AclI AA'CG_TT
Cuts at: 1563 2825 5748 6121 1563
Size: 1262 2923 373 3063
Fragments arranged by size:
3063 2923 1262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5684 7068 7336 1632
Size: 4052 1384 268 1917
Fragments arranged by size:
4052 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2311 820
Size: 197 1294 6130
Fragments arranged by size:
6130 1294 197
AflIII A'CryG_T
Cuts at: 1203 2289 4530 6520 7497 1203
Size: 1086 2241 1990 977 1327
Fragments arranged by size:
2241 1990 1327 1086 977
AgeI A'CCGG_T
Cuts at: 6452 6452
Size: 7621
AhdI GACnn_n'nnGTC
Cuts at: 4549 6350 4549
Size: 1801 5820
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2603 1184
Size: 1419 6202
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3903 3935 3903
Size: 32 7589
AlwNI CAG_nnn'CTG
Cuts at: 3341 4091 4900 5364 6936 3341
Size: 750 809 464 1572 4026
Fragments arranged by size:
4026 1572 809 750 464
ApaI G_GGCC'C
Cuts at: 3987 3987
Size: 7621
ApaLI G'TGCA_C
Cuts at: 2293 2701 4907 5678 6834 2293
Size: 408 2206 771 1156 3080
Fragments arranged by size:
3080 2206 1156 771 408
ApoI r'AATT_y
Cuts at: 1493 2600 2687 4030 4930 5156 5308 7417 1493
Size: 1107 87 1343 900 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 1107 900 226 152 87
AseI AT'TA_AT
Cuts at: 160 1032 2943 6175 160
Size: 872 1911 3232 1606
Fragments arranged by size:
3232 1911 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7621
AvaI C'yCGr_G
Cuts at: 1870 1943 4946 1870
Size: 73 3003 4545
Fragments arranged by size:
4545 3003 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3112 3145 3112
Size: 33 7588
BamHI G'GATC_C
Cuts at: 4951 5318 4951
Size: 367 7254
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2454 3155 4501 4942
Size: 325 217 627 234 433 701 1346 441
Cuts at: 4942 6397 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1346 701 627 441 433 325
234 217
BanII G_rGCy'C
Cuts at: 1391 3312 3987 4940 1391
Size: 1921 675 953 4072
Fragments arranged by size:
4072 1921 953 675
BbeI G_GCGC'C
Cuts at: 2025 2025
Size: 7621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2394 4119 4299 4709 928
Size: 971 495 1725 180 410 3840
Fragments arranged by size:
3840 1725 971 495 410 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5812 5846 1903
Size: 34 3875 34 3678
Fragments arranged by size:
3875 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5513 6723 5513
Size: 1210 6411
BclI T'GATC_A
Cuts at: 1548 2517 3727 4638 1548
Size: 969 1210 911 4531
Fragments arranged by size:
4531 1210 969 911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6232 136
Size: 107 122 71 631 605 4560 1525
Fragments arranged by size:
4560 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4211 7616 4211
Size: 3405 4216
BlpI GC'TnA_GC
Cuts at: 4301 4661 5038 4301
Size: 360 377 6884
Fragments arranged by size:
6884 377 360
Bme1580I G_kGCm'C
Cuts at: 1790 2297 2705 3880 3987 4911 5682 6838 1790
Size: 507 408 1175 107 924 771 1156 2573
Fragments arranged by size:
2573 1175 1156 924 771 507 408 107
BmgBI CAC'GTC
Cuts at: 1318 2700 1318
Size: 1382 6239
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5352 6310 442
Size: 416 53 83 399 24 3935 958 1753
Fragments arranged by size:
3935 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7621
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 4597 2031
Size: 2566 5055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2880 3804 4191 6281 1239
Size: 1641 924 387 2090 2579
Fragments arranged by size:
2579 2090 1641 924 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2432 2503 3851 5746 6611 6909 2432
Size: 71 1348 1895 865 298 3144
Fragments arranged by size:
3144 1895 1348 865 298 71
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6284 882
Size: 617 4785 2219
Fragments arranged by size:
4785 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2292 3124 5412 493
Size: 711 1088 832 2288 2702
Fragments arranged by size:
2702 2288 1088 832 711
BsaBI GATnn'nnATC
Cuts at: 4719 7615 4719
Size: 2896 4725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 3198 4545 5428
Size: 53 83 186 936 489 1176 1347 883
Cuts at: 5428 5810 6445 275
Size: 382 635 1451
Fragments arranged by size:
1451 1347 1176 936 883 635 489 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4653 6054 6452 6726 6873 7284 4653
Size: 1401 398 274 147 411 4990
Fragments arranged by size:
4990 1401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2567 2597 2979 3009 1178
Size: 30 165 30 1164 30 382 30 5790
Fragments arranged by size:
5790 1164 382 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3689 4879 1186
Size: 2503 1190 3928
Fragments arranged by size:
3928 2503 1190
BseYI C'CCAG_C
Cuts at: 4710 4893 6824 7320 4710
Size: 183 1931 496 5011
Fragments arranged by size:
5011 1931 496 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3401 3955 4077 1163
Size: 573 1665 554 122 4707
Fragments arranged by size:
4707 1665 573 554 122
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4617 5071 5832 6860 1540
Size: 275 186 2616 454 761 1028 2301
Fragments arranged by size:
2616 2301 1028 761 454 275 186
BsiHKAI G_wGCw'C
Cuts at: 2297 2705 4787 4911 5682 5767 6838 2297
Size: 408 2082 124 771 85 1071 3080
Fragments arranged by size:
3080 2082 1071 771 408 124 85
BsmI GAATG_Cn'
Cuts at: 3020 3449 4114 4614 5132 5225 3020
Size: 429 665 500 518 93 5416
Fragments arranged by size:
5416 665 518 500 429 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2112 2112
Size: 7621
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2297 2705 3312 3880 3987 4787 4911
Size: 399 507 408 607 568 107 800 124
Cuts at: 4911 4940 5682 5767 6838 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 800 742 607 568 507 408
399 124 107 85 29
BspEI T'CCGG_A
Cuts at: 4653 7284 4653
Size: 2631 4990
BspHI T'CATG_A
Cuts at: 2514 5510 7240 2514
Size: 2996 1730 2895
Fragments arranged by size:
2996 2895 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4687 4976 844
Size: 3843 289 3489
Fragments arranged by size:
3843 3489 289
BsrBI CCG'CTC
Cuts at: 1394 1823 2370 5508 1394
Size: 429 547 3138 3507
Fragments arranged by size:
3507 3138 547 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6116 6290 65
Size: 1183 4868 174 1396
Fragments arranged by size:
4868 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2279 4612 6265 6452 1700
Size: 110 469 2333 1653 187 2869
Fragments arranged by size:
2869 2333 1653 469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7621
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7621
BssSI C'ACGA_G
Cuts at: 3266 5681 6693 3266
Size: 2415 1012 4194
Fragments arranged by size:
4194 2415 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3600 4091 794
Size: 2806 491 4324
Fragments arranged by size:
4324 2806 491
BstBI TT'CG_AA
Cuts at: 6425 6425
Size: 7621
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7621
BstYI r'GATC_y
Cuts at: 1972 2211 2341 3048 4211 4720 4951 5318 5703
Size: 239 130 707 1163 509 231 367 385
Cuts at: 5703 5720 7172 7258 7270 7616 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1163 707 509 385 367 346
239 231 130 86 17 12
BstZ17I GTA'TAC
Cuts at: 4528 4528
Size: 7621
BtgI C'CryG_G
Cuts at: 513 1065 4839 513
Size: 552 3774 3295
Fragments arranged by size:
3774 3295 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6882
Fragments arranged by size:
6882 683 56
BtsI GCAGTG_nn'
Cuts at: 3050 5137 5931 5951 3050
Size: 2087 794 20 4720
Fragments arranged by size:
4720 2087 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7621
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7586
DraI TTT'AAA
Cuts at: 3529 4925 3529
Size: 1396 6225
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2144 2402 6628 809
Size: 507 828 258 4226 1802
Fragments arranged by size:
4226 1802 828 507 258
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3840 5068 5957
Size: 54 1425 50 275 18 2010 1228 889
Cuts at: 5957 7586 8
Size: 1629 43
Fragments arranged by size:
2010 1629 1425 1228 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5068 1537
Size: 275 3256 4090
Fragments arranged by size:
4090 3256 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2080 2475 5551 1353
Size: 727 395 3076 3423
Fragments arranged by size:
3423 3076 727 395
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6208 6582 6728 387
Size: 1064 138 4619 374 146 1280
Fragments arranged by size:
4619 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2880 3804 4191 5684 6281 7068 7336
Size: 393 1248 924 387 1493 597 787 268
Cuts at: 7336 1239
Size: 1524
Fragments arranged by size:
1524 1493 1248 924 787 597 393 387
268
EcoO109I rG'GnC_Cy
Cuts at: 3983 3983
Size: 7621
EcoRI G'AATT_C
Cuts at: 2687 4930 5308 2687
Size: 2243 378 5000
Fragments arranged by size:
5000 2243 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4112 4666 4112
Size: 554 7067
FspI TGC'GCA
Cuts at: 6127 6127
Size: 7621
HaeII r_GCGC'y
Cuts at: 1678 2016 2025 6768 1678
Size: 338 9 4743 2531
Fragments arranged by size:
4743 2531 338 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2567 2599 4535 4567 4774
Size: 32 135 32 995 32 1936 32 207
Cuts at: 4774 4806 6262 6294 6336 6368 1373
Size: 32 1456 32 42 32 2626
Fragments arranged by size:
2626 1936 1456 995 207 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2365 2829 4965 5211 669
Size: 988 281 427 464 2136 246 3079
Fragments arranged by size:
3079 2136 988 464 427 281 246
HindIII A'AGCT_T
Cuts at: 748 2351 748
Size: 1603 6018
HpaI GTT'AAC
Cuts at: 5211 5211
Size: 7621
KasI G'GCGC_C
Cuts at: 2021 2021
Size: 7621
KpnI G_GTAC'C
Cuts at: 4946 4946
Size: 7621
MfeI C'AATT_G
Cuts at: 5220 5220
Size: 7621
MluI A'CGCG_T
Cuts at: 7497 7497
Size: 7621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2574 4382 4827 6735 6919 1305
Size: 1269 1808 445 1908 184 2007
Fragments arranged by size:
2007 1908 1808 1269 445 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5799
Fragments arranged by size:
5799 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 2796 3608 4160 4914 5579 5938
Size: 648 1161 469 812 552 754 665 359
Cuts at: 5938 6097 518
Size: 159 2042
Fragments arranged by size:
2042 1161 812 754 665 648 552 469
359 159
MspA1I CmG'CkG
Cuts at: 1606 2409 5037 5714 6862 7107 1606
Size: 803 2628 677 1148 245 2120
Fragments arranged by size:
2628 2120 1148 803 677 245
NarI GG'CG_CC
Cuts at: 2022 2022
Size: 7621
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7069
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7621
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7621
NotI GC'GGCC_GC
Cuts at: 5068 5068
Size: 7621
NspI r_CATG'y
Cuts at: 1132 1229 3280 4979 6524 1132
Size: 97 2051 1699 1545 2229
Fragments arranged by size:
2229 2051 1699 1545 97
PacI TTA_AT'TAA
Cuts at: 3828 3828
Size: 7621
PasI CC'CwG_GG
Cuts at: 1348 3775 1348
Size: 2427 5194
PciI A'CATG_T
Cuts at: 6520 6520
Size: 7621
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3289 3410 1108
Size: 2181 121 5319
Fragments arranged by size:
5319 2181 121
PfoI T'CCnGG_A
Cuts at: 2125 2214 3334 4603 2125
Size: 89 1120 1269 5143
Fragments arranged by size:
5143 1269 1120 89
PmeI GTTT'AAAC
Cuts at: 4925 4925
Size: 7621
PmlI CAC'GTG
Cuts at: 2292 2292
Size: 7621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5690 5722 7203 7235 5690
Size: 32 1481 32 6076
Fragments arranged by size:
6076 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7621
PsiI TTA'TAA
Cuts at: 4101 5191 4101
Size: 1090 6531
PspOMI G'GGCC_C
Cuts at: 3983 3983
Size: 7621
PstI C_TGCA'G
Cuts at: 830 4136 4820 4973 830
Size: 3306 684 153 3478
Fragments arranged by size:
3478 3306 684 153
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7621
SalI G'TCGA_C
Cuts at: 1936 4963 1936
Size: 3027 4594
SapI GCTCTTCn'nnn_
Cuts at: 2080 2080
Size: 7621
SbfI CC_TGCA'GG
Cuts at: 4973 4973
Size: 7621
ScaI AGT'ACT
Cuts at: 4144 5869 7581 4144
Size: 1725 1712 4184
Fragments arranged by size:
4184 1725 1712
SexAI A'CCwGG_T
Cuts at: 4314 4314
Size: 7621
SfcI C'TryA_G
Cuts at: 826 1045 2768 2967 3435 4132 4816 4969 6104
Size: 219 1723 199 468 697 684 153 1135
Cuts at: 6104 6785 6976 826
Size: 681 191 1471
Fragments arranged by size:
1723 1471 1135 697 684 681 468 219
199 191 153
SfoI GGC'GCC
Cuts at: 2023 2023
Size: 7621
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7621
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7621
SmaI CCC'GGG
Cuts at: 1872 4948 1872
Size: 3076 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2311 2447 2482 3866 5725 6626
Size: 197 926 368 136 35 1384 1859 901
Cuts at: 6626 6888 820
Size: 262 1553
Fragments arranged by size:
1859 1553 1384 926 901 368 262 197
136 35
SnaBI TAC'GTA
Cuts at: 493 3124 5412 493
Size: 2631 2288 2702
Fragments arranged by size:
2702 2631 2288
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7621
SphI G_CATG'C
Cuts at: 1132 3280 4979 1132
Size: 2148 1699 3774
Fragments arranged by size:
3774 2148 1699
SspI AAT'ATT
Cuts at: 5 52 3632 3949 4007 5545 5
Size: 47 3580 317 58 1538 2081
Fragments arranged by size:
3580 2081 1538 317 58 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7621
StyI C'CwwG_G
Cuts at: 513 1065 2475 2502 2916 3931 4194 5060 513
Size: 552 1410 27 414 1015 263 866 3074
Fragments arranged by size:
3074 1410 1015 866 552 414 263 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3767 5648 5665 5818 6003 6448 325
Size: 1255 2187 1881 17 153 185 445 1498
Fragments arranged by size:
2187 1881 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2730 3364 3655
Size: 274 80 33 51 1443 753 634 291
Cuts at: 3655 4142 4152 5867 7579 96
Size: 487 10 1715 1712 138
Fragments arranged by size:
1715 1712 1443 753 634 487 291 274
138 80 51 33 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2103 2135 3405 3437 7581 7613 2103
Size: 32 1270 32 4144 32 2111
Fragments arranged by size:
4144 2111 1270 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4916 1316
Size: 3600 4021
XbaI T'CTAG_A
Cuts at: 4957 4957
Size: 7621
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7621
XmaI C'CCGG_G
Cuts at: 1870 4946 1870
Size: 3076 4545
XmnI GAAnn'nnTTC
Cuts at: 5750 5750
Size: 7621
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3199 4546 5429 276
Size: 53 83 186 936 1665 1347 883 2468
Fragments arranged by size:
2468 1665 1347 936 883 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstXI
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PpuMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SfiI SrfI SwaI XcmI