Restriction map for
pFN21AB7005
(Circular) (Six-base) MAPSORT of: pFN21AB7005.seq Check: 8842 from: 1 to: 8482
pFN21AB7005 8482 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3956 5490 6292 278
Size: 53 83 186 936 2420 1534 802 2468
Fragments arranged by size:
2468 2420 1534 936 802 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 4262 4527 5141 5355 5577 5825 1937
Size: 1473 852 265 614 214 222 248 4594
Fragments arranged by size:
4594 1473 852 614 265 248 222 214
Acc65I G'GTAC_C
Cuts at: 5803 5803
Size: 8482
AclI AA'CG_TT
Cuts at: 1563 6609 6982 1563
Size: 5046 373 3063
Fragments arranged by size:
5046 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2369 3050 3681 3880 4647 4810 4822 6545
Size: 737 681 631 199 767 163 12 1723
Cuts at: 6545 7929 8197 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1723 1384 767 737 681 631 268
199 163 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8285
AflIII A'CryG_T
Cuts at: 1203 4341 7381 8358 1203
Size: 3138 3040 977 1327
Fragments arranged by size:
3138 3040 1327 977
AgeI A'CCGG_T
Cuts at: 4949 7313 4949
Size: 2364 6118
AhdI GACnn_n'nnGTC
Cuts at: 5569 7211 5569
Size: 1642 6840
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2972 3774 4619 1184
Size: 1788 802 845 5047
Fragments arranged by size:
5047 1788 845 802
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4976 5008 4976
Size: 32 8450
AlwNI CAG_nnn'CTG
Cuts at: 3028 5108 5417 5689 6225 7797 3028
Size: 2080 309 272 536 1572 3713
Fragments arranged by size:
3713 2080 1572 536 309 272
ApaLI G'TGCA_C
Cuts at: 2947 6539 7695 2947
Size: 3592 1156 3734
Fragments arranged by size:
3734 3592 1156
ApoI r'AATT_y
Cuts at: 1493 2086 4634 4900 5150 5164 5791 6017 6169
Size: 593 2548 266 250 14 627 226 152
Cuts at: 6169 8278 1493
Size: 2109 1697
Fragments arranged by size:
2548 2109 1697 627 593 266 250 226
152 14
AseI AT'TA_AT
Cuts at: 160 1032 7036 160
Size: 872 6004 1606
Fragments arranged by size:
6004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8482
AvaI C'yCGr_G
Cuts at: 1870 1943 3980 5421 5719 5807 1870
Size: 73 2037 1441 298 88 4545
Fragments arranged by size:
4545 2037 1441 298 88 73
AvrII C'CTAG_G
Cuts at: 2596 2596
Size: 8482
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2283 2316 2283
Size: 33 8449
BamHI G'GATC_C
Cuts at: 5812 6179 5812
Size: 367 8115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2798 4172 5803 7258 618
Size: 325 217 627 1011 1374 1631 1455 1842
Fragments arranged by size:
1842 1631 1455 1374 1011 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2170 4295 4359 4713 5801 1391
Size: 779 2125 64 354 1088 4072
Fragments arranged by size:
4072 2125 1088 779 354 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3929 4542 5698 928
Size: 971 2030 613 1156 3712
Fragments arranged by size:
3712 2030 1156 971 613
BbvCI CC'TCA_GC
Cuts at: 3537 4184 3537
Size: 647 7835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6673 6707 1903
Size: 34 4736 34 3678
Fragments arranged by size:
4736 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4012 6374 7584 4012
Size: 2362 1210 4910
Fragments arranged by size:
4910 2362 1210
BclI T'GATC_A
Cuts at: 1548 5259 1548
Size: 3711 4771
BfrBI ATG'CAT
Cuts at: 3751 3751
Size: 8482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7093 136
Size: 107 122 71 631 605 5421 1525
Fragments arranged by size:
5421 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2917 3247 3454 3772 5196 8477 2917
Size: 330 207 318 1424 3281 2922
Fragments arranged by size:
3281 2922 1424 330 318 207
BlpI GC'TnA_GC
Cuts at: 3288 4472 5899 3288
Size: 1184 1427 5871
Fragments arranged by size:
5871 1427 1184
Bme1580I G_kGCm'C
Cuts at: 1790 2951 3133 3550 4177 5726 6543 7699 1790
Size: 1161 182 417 627 1549 817 1156 2573
Fragments arranged by size:
2573 1549 1161 1156 817 627 417 182
BmgBI CAC'GTC
Cuts at: 1318 4342 5761 1318
Size: 3024 1419 4039
Fragments arranged by size:
4039 3024 1419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4089 5380 6213
Size: 416 53 83 399 24 2672 1291 833
Cuts at: 6213 7171 442
Size: 958 1753
Fragments arranged by size:
2672 1753 1291 958 833 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8482
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4147 4165 4147
Size: 18 8464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2097 2674 3113 4046 4184 5270 5698 7142
Size: 858 577 439 933 138 1086 428 1444
Cuts at: 7142 1239
Size: 2579
Fragments arranged by size:
2579 1444 1086 933 858 577 439 428
138
Bpu10I CC'TnA_GC
Cuts at: 3537 4184 3537
Size: 647 7835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2647 5027 5600 6607 7472 7770 2647
Size: 2380 573 1007 865 298 3359
Fragments arranged by size:
3359 2380 1007 865 573 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3977 7145 882
Size: 617 2478 3168 2219
Fragments arranged by size:
3168 2478 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3808 3868 4180 4198 4369 4627 6273
Size: 711 2604 60 312 18 171 258 1646
Cuts at: 6273 493
Size: 2702
Fragments arranged by size:
2702 2604 1646 711 312 258 171 60
18
BsaBI GATnn'nnATC
Cuts at: 4935 8476 4935
Size: 3541 4941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2727 2754 3953
Size: 53 83 186 936 477 717 27 1199
Cuts at: 3953 5487 6289 6671 7306 275
Size: 1534 802 382 635 1451
Fragments arranged by size:
1534 1451 1199 936 802 717 635 477
382 186 83 53 27
BsaWI w'CCGG_w
Cuts at: 3918 4566 4949 6915 7313 7587 7734 8145 3918
Size: 648 383 1966 398 274 147 411 4255
Fragments arranged by size:
4255 1966 648 411 398 383 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2647 2677 4839 4869 1178
Size: 30 165 30 1244 30 2162 30 4791
Fragments arranged by size:
4791 2162 1244 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2228 2699 3680 4123 4181 5518 1186
Size: 847 195 471 981 443 58 1337 4150
Fragments arranged by size:
4150 1337 981 847 471 443 195 58
BseYI C'CCAG_C
Cuts at: 2988 3072 3543 4359 4669 4696 4995 5101 5744
Size: 84 471 816 310 27 299 106 643
Cuts at: 5744 7685 8181 2988
Size: 1941 496 3289
Fragments arranged by size:
3289 1941 816 643 496 471 310 299
106 84 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2756 2993 3041 4210 4349 4352 5505
Size: 573 1020 237 48 1169 139 3 1153
Cuts at: 5505 1163
Size: 4140
Fragments arranged by size:
4140 1169 1153 1020 573 237 139 48
3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5697 5932 6693 7721 1540
Size: 275 186 3696 235 761 1028 2301
Fragments arranged by size:
3696 2301 1028 761 275 235 186
BsiHKAI G_wGCw'C
Cuts at: 2499 2951 3064 3520 5658 6543 6628 7699 2499
Size: 452 113 456 2138 885 85 1071 3282
Fragments arranged by size:
3282 2138 1071 885 456 452 113 85
BsmI GAATG_Cn'
Cuts at: 2548 3751 4401 5635 5993 6086 2548
Size: 1203 650 1234 358 93 4944
Fragments arranged by size:
4944 1234 1203 650 358 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2170 2499 2951 3064 3133 3520 3550
Size: 399 380 329 452 113 69 387 30
Cuts at: 3550 4177 4295 4359 4713 5658 5726 5801 6543
Size: 627 118 64 354 945 68 75 742
Cuts at: 6543 6628 7699 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 945 742 627 452 399 387
380 354 329 118 113 85 75 69
68 64 30
BspEI T'CCGG_A
Cuts at: 4566 8145 4566
Size: 3579 4903
BspHI T'CATG_A
Cuts at: 6371 8101 6371
Size: 1730 6752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3266 3627 4974 5837 844
Size: 2422 361 1347 863 3489
Fragments arranged by size:
3489 2422 1347 863 361
BsrBI CCG'CTC
Cuts at: 1394 1823 5458 6369 1394
Size: 429 3635 911 3507
Fragments arranged by size:
3635 3507 911 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2567 3147 4483 5074 6977 7151 65
Size: 1183 1319 580 1336 591 1903 174 1396
Fragments arranged by size:
1903 1396 1336 1319 1183 591 580 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2032 4433 4949 5079 5692 5696 7126
Size: 110 222 2401 516 130 613 4 1430
Cuts at: 7126 7313 1700
Size: 187 2869
Fragments arranged by size:
2869 2401 1430 613 516 222 187 130
110 4
BsrGI T'GTAC_A
Cuts at: 96 3840 96
Size: 3744 4738
BssHII G'CGCG_C
Cuts at: 1925 2992 1925
Size: 1067 7415
BssSI C'ACGA_G
Cuts at: 4537 4591 5263 6542 7554 4537
Size: 54 672 1279 1012 5465
Fragments arranged by size:
5465 1279 1012 672 54
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5689 794
Size: 4895 3587
BstBI TT'CG_AA
Cuts at: 7286 7286
Size: 8482
BstEII G'GTnAC_C
Cuts at: 1184 4756 1184
Size: 3572 4910
BstXI CCAn_nnnn'nTGG
Cuts at: 2145 2872 3799 5413 2145
Size: 727 927 1614 5214
Fragments arranged by size:
5214 1614 927 727
BstYI r'GATC_y
Cuts at: 1972 2917 3247 3454 3772 5196 5812 6179 6564
Size: 945 330 207 318 1424 616 367 385
Cuts at: 6564 6581 8033 8119 8131 8477 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1424 945 616 385 367 346
330 318 207 86 17 12
BstZ17I GTA'TAC
Cuts at: 3411 4263 5142 3411
Size: 852 879 6751
Fragments arranged by size:
6751 879 852
Bsu36I CC'TnA_GG
Cuts at: 4150 4864 4150
Size: 714 7768
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 2975 3798 4790 513
Size: 552 939 42 929 823 992 4205
Fragments arranged by size:
4205 992 939 929 823 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4798 487
Size: 683 56 3572 4171
Fragments arranged by size:
4171 3572 683 56
BtsI GCAGTG_nn'
Cuts at: 5382 5687 5998 6792 6812 5382
Size: 305 311 794 20 7052
Fragments arranged by size:
7052 794 311 305 20
ClaI AT'CG_AT
Cuts at: 1554 2888 1554
Size: 1334 7148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8447
DraI TTT'AAA
Cuts at: 5155 5786 5155
Size: 631 7851
DraIII CAC_nnn'GTG
Cuts at: 2334 3059 3160 4774 2334
Size: 725 101 1614 6042
Fragments arranged by size:
6042 1614 725 101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7489 809
Size: 507 6173 1802
Fragments arranged by size:
6173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 3579 4435
Size: 54 1425 50 275 18 331 1418 856
Cuts at: 4435 4744 5694 5698 5929 6818 8447 8
Size: 309 950 4 231 889 1629 43
Fragments arranged by size:
1629 1425 1418 950 889 856 331 309
275 231 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 5694 5929 1537
Size: 275 3882 235 4090
Fragments arranged by size:
4090 3882 275 235
EarI CTCTTCn'nnn_
Cuts at: 1353 2524 2630 2765 2828 2966 3657 5049 5465
Size: 1171 106 135 63 138 691 1392 416
Cuts at: 5465 6412 1353
Size: 947 3423
Fragments arranged by size:
3423 1392 1171 947 691 416 138 135
106 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4523 7069 7443 7589 387
Size: 1064 138 2934 2546 374 146 1280
Fragments arranged by size:
2934 2546 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2369 2674 3050 3113 3681 3880
Size: 393 465 272 305 376 63 568 199
Cuts at: 3880 4046 4184 4647 4810 4822 5270 5698 6545
Size: 166 138 463 163 12 448 428 847
Cuts at: 6545 7142 7929 8197 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 847 787 597 568 465 463 448
428 393 376 305 272 268 199 166
163 138 63 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3536 4502 5282 3536
Size: 966 780 6736
Fragments arranged by size:
6736 966 780
EcoO109I rG'GnC_Cy
Cuts at: 2154 3233 2154
Size: 1079 7403
EcoRI G'AATT_C
Cuts at: 5791 6169 5791
Size: 378 8104
FseI GG_CCGG'CC
Cuts at: 5700 5700
Size: 8482
FspI TGC'GCA
Cuts at: 2924 6988 2924
Size: 4064 4418
HaeII r_GCGC'y
Cuts at: 1678 7629 1678
Size: 5951 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2838 2870 4331 4363 4585
Size: 32 135 32 1266 32 1461 32 222
Cuts at: 4585 4617 5555 5587 7123 7155 7197 7229 1373
Size: 32 938 32 1536 32 42 32 2626
Fragments arranged by size:
2626 1536 1461 1266 938 222 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2779 3265 5356 5826 6072 669
Size: 988 281 841 486 2091 470 246 3079
Fragments arranged by size:
3079 2091 988 841 486 470 281 246
HindIII A'AGCT_T
Cuts at: 748 3360 5336 748
Size: 2612 1976 3894
Fragments arranged by size:
3894 2612 1976
HpaI GTT'AAC
Cuts at: 6072 6072
Size: 8482
KpnI G_GTAC'C
Cuts at: 5807 5807
Size: 8482
MfeI C'AATT_G
Cuts at: 6081 6081
Size: 8482
MluI A'CGCG_T
Cuts at: 8358 8358
Size: 8482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2132 2526 3427 4557 4605 5071 7596 7780
Size: 827 394 901 1130 48 466 2525 184
Cuts at: 7780 1305
Size: 2007
Fragments arranged by size:
2525 2007 1130 901 827 466 394 184
48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6660
Fragments arranged by size:
6660 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2870 3404 5405 5411 5447 6440 6799
Size: 648 1704 534 2001 6 36 993 359
Cuts at: 6799 6958 518
Size: 159 2042
Fragments arranged by size:
2042 2001 1704 993 648 534 359 159
36 6
MspA1I CmG'CkG
Cuts at: 1606 2026 3577 4276 4363 4378 4749 4924 5119
Size: 420 1551 699 87 15 371 175 195
Cuts at: 5119 5550 5898 6575 7723 7968 1606
Size: 431 348 677 1148 245 2120
Fragments arranged by size:
2120 1551 1148 699 677 431 420 371
348 245 195 175 87 15
NaeI GCC'GGC
Cuts at: 5694 5698 5694
Size: 4 8478
NcoI C'CATG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 6949
Fragments arranged by size:
6949 939 552 42
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8482
NgoMIV G'CCGG_C
Cuts at: 5692 5696 5692
Size: 4 8478
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8482
NotI GC'GGCC_GC
Cuts at: 5929 5929
Size: 8482
NsiI A_TGCA'T
Cuts at: 3753 3753
Size: 8482
NspI r_CATG'y
Cuts at: 1132 1229 4108 4696 5840 7385 1132
Size: 97 2879 588 1144 1545 2229
Fragments arranged by size:
2879 2229 1545 1144 588 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8482
PciI A'CATG_T
Cuts at: 7381 7381
Size: 8482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3160 5764 1108
Size: 2052 2604 3826
Fragments arranged by size:
3826 2604 2052
PfoI T'CCnGG_A
Cuts at: 2537 5763 2537
Size: 3226 5256
PmeI GTTT'AAAC
Cuts at: 5786 5786
Size: 8482
PmlI CAC'GTG
Cuts at: 3808 3868 4180 4198 4369 4627 3808
Size: 60 312 18 171 258 7663
Fragments arranged by size:
7663 312 258 171 60 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3018 3050 6551 6583 8064 8096 3018
Size: 32 3501 32 1481 32 3404
Fragments arranged by size:
3501 3404 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2154 3233 2154
Size: 1079 7403
PshAI GACnn'nnGTC
Cuts at: 1613 3220 1613
Size: 1607 6875
PsiI TTA'TAA
Cuts at: 3327 6052 3327
Size: 2725 5757
PstI C_TGCA'G
Cuts at: 830 2715 2970 3624 4094 4470 5648 5685 5834
Size: 1885 255 654 470 376 1178 37 149
Cuts at: 5834 830
Size: 3478
Fragments arranged by size:
3478 1885 1178 654 470 376 255 149
37
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8482
PvuII CAG'CTG
Cuts at: 3577 4276 4363 4924 5119 5550 3577
Size: 699 87 561 195 431 6509
Fragments arranged by size:
6509 699 561 431 195 87
SalI G'TCGA_C
Cuts at: 1936 5354 5824 1936
Size: 3418 470 4594
Fragments arranged by size:
4594 3418 470
SapI GCTCTTCn'nnn_
Cuts at: 2828 2966 5049 5465 2828
Size: 138 2083 416 5845
Fragments arranged by size:
5845 2083 416 138
SbfI CC_TGCA'GG
Cuts at: 5834 5834
Size: 8482
ScaI AGT'ACT
Cuts at: 6730 8442 6730
Size: 1712 6770
SexAI A'CCwGG_T
Cuts at: 3055 3055
Size: 8482
SfcI C'TryA_G
Cuts at: 826 1045 2310 2711 2966 3620 4090 4466 5008
Size: 219 1265 401 255 654 470 376 542
Cuts at: 5008 5644 5681 5830 6965 7646 7837 826
Size: 636 37 149 1135 681 191 1471
Fragments arranged by size:
1471 1265 1135 681 654 636 542 470
401 376 255 219 191 149 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8482
SgrAI Cr'CCGG_yG
Cuts at: 1700 2032 1700
Size: 332 8150
SmaI CCC'GGG
Cuts at: 1872 5809 1872
Size: 3937 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2626 5042 5615 6586 7487 7749
Size: 197 926 683 2416 573 971 901 262
Cuts at: 7749 820
Size: 1553
Fragments arranged by size:
2416 1553 971 926 901 683 573 262
197
SnaBI TAC'GTA
Cuts at: 493 6273 493
Size: 5780 2702
SpeI A'CTAG_T
Cuts at: 152 4972 152
Size: 4820 3662
SphI G_CATG'C
Cuts at: 1132 5840 1132
Size: 4708 3774
SspI AAT'ATT
Cuts at: 5 52 2669 6406 5
Size: 47 2617 3737 2081
Fragments arranged by size:
3737 2617 2081 47
StuI AGG'CCT
Cuts at: 1517 5664 1517
Size: 4147 4335
StyI C'CwwG_G
Cuts at: 513 1065 2004 2046 2596 3198 3598 4245 5921
Size: 552 939 42 550 602 400 647 1676
Cuts at: 5921 513
Size: 3074
Fragments arranged by size:
3074 1676 939 647 602 552 550 400
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3064 6509 6526 6679 6864 7309 325
Size: 1255 1484 3445 17 153 185 445 1498
Fragments arranged by size:
3445 1498 1484 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3840 4344 6728
Size: 274 80 33 51 1443 1863 504 2384
Cuts at: 6728 8440 96
Size: 1712 138
Fragments arranged by size:
2384 1863 1712 1443 504 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2647 2679 3938 3970 4839 4871 5030 5062 8442
Size: 32 1259 32 869 32 159 32 3380
Cuts at: 8442 8474 2647
Size: 32 2655
Fragments arranged by size:
3380 2655 1259 869 159 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4586 5759 1316
Size: 3270 1173 4039
Fragments arranged by size:
4039 3270 1173
XbaI T'CTAG_A
Cuts at: 4138 5818 4138
Size: 1680 6802
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2835 2835
Size: 8482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8482
XmaI C'CCGG_G
Cuts at: 1870 5807 1870
Size: 3937 4545
XmnI GAAnn'nnTTC
Cuts at: 2546 6611 2546
Size: 4065 4417
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3954 5488 6290 276
Size: 53 83 186 936 2420 1534 802 2468
Fragments arranged by size:
2468 2420 1534 936 802 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BbeI BsiWI BsmBI EcoICRI
EcoRV FalI FspAI KasI NarI NruI PacI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI