Restriction map for
pFN21AB6996
(Circular) (Six-base) MAPSORT of: pFN21AB6996.seq Check: 2665 from: 1 to: 5467
pFN21AB6996 5467 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3277 278
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2810 1937
Size: 873 4594
Acc65I G'GTAC_C
Cuts at: 2788 2788
Size: 5467
AclI AA'CG_TT
Cuts at: 1563 3594 3967 1563
Size: 2031 373 3063
Fragments arranged by size:
3063 2031 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2460 2514 3530 4914 5182 1632
Size: 828 54 1016 1384 268 1917
Fragments arranged by size:
1917 1384 1016 828 268 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5270
AflIII A'CryG_T
Cuts at: 1203 2178 4366 5343 1203
Size: 975 2188 977 1327
Fragments arranged by size:
2188 1327 977 975
AgeI A'CCGG_T
Cuts at: 4298 4298
Size: 5467
AhdI GACnn_n'nnGTC
Cuts at: 4196 4196
Size: 5467
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5467
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2226 2258 2226
Size: 32 5435
AlwNI CAG_nnn'CTG
Cuts at: 2113 2492 3210 4782 2113
Size: 379 718 1572 2798
Fragments arranged by size:
2798 1572 718 379
ApaLI G'TGCA_C
Cuts at: 3524 4680 3524
Size: 1156 4311
ApoI r'AATT_y
Cuts at: 1493 2395 2673 2776 3002 3154 5263 1493
Size: 902 278 103 226 152 2109 1697
Fragments arranged by size:
2109 1697 902 278 226 152 103
AseI AT'TA_AT
Cuts at: 160 1032 4021 160
Size: 872 2989 1606
Fragments arranged by size:
2989 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
AvaI C'yCGr_G
Cuts at: 1870 1943 2792 1870
Size: 73 849 4545
Fragments arranged by size:
4545 849 73
BamHI G'GATC_C
Cuts at: 2797 3164 2797
Size: 367 5100
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2788 4243 618
Size: 325 217 627 1001 1455 1842
Fragments arranged by size:
1842 1455 1001 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2786 1391
Size: 1395 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2332 928
Size: 971 433 4063
Fragments arranged by size:
4063 971 433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3658 3692 1903
Size: 34 1721 34 3678
Fragments arranged by size:
3678 1721 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3359 4569 3359
Size: 1210 4257
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4078 136
Size: 107 122 71 631 605 337 2069 1525
Fragments arranged by size:
2069 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5462 5462
Size: 5467
BlpI GC'TnA_GC
Cuts at: 2884 2884
Size: 5467
Bme1580I G_kGCm'C
Cuts at: 1790 3528 4684 1790
Size: 1738 1156 2573
Fragments arranged by size:
2573 1738 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5467
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2162 3198 4156
Size: 416 53 83 399 24 745 1036 958
Cuts at: 4156 442
Size: 1753
Fragments arranged by size:
1753 1036 958 745 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2606 4127 1239
Size: 1367 1521 2579
Fragments arranged by size:
2579 1521 1367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 3592 4457 4755 2267
Size: 1325 865 298 2979
Fragments arranged by size:
2979 1325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4130 882
Size: 617 2631 2219
Fragments arranged by size:
2631 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3258 493
Size: 711 2054 2702
Fragments arranged by size:
2702 2054 711
BsaBI GATnn'nnATC
Cuts at: 5461 5461
Size: 5467
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3274 3656 4291 275
Size: 53 83 186 936 1741 382 635 1451
Fragments arranged by size:
1741 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2701 3900 4298 4572 4719 5130 2701
Size: 1199 398 274 147 411 3038
Fragments arranged by size:
3038 1199 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2562 2592 1178
Size: 30 165 30 1159 30 4053
Fragments arranged by size:
4053 1159 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5467
BseYI C'CCAG_C
Cuts at: 2028 2275 4670 5166 2028
Size: 247 2395 496 2329
Fragments arranged by size:
2395 2329 496 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2579 1163
Size: 573 843 4051
Fragments arranged by size:
4051 843 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2917 3678 4706 1540
Size: 275 186 916 761 1028 2301
Fragments arranged by size:
2301 1028 916 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3528 3613 4684 3528
Size: 85 1071 4311
Fragments arranged by size:
4311 1071 85
BsmI GAATG_Cn'
Cuts at: 2978 3071 2978
Size: 93 5374
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2786 3528 3613 4684 1391
Size: 399 996 742 85 1071 2174
Fragments arranged by size:
2174 1071 996 742 399 85
BspEI T'CCGG_A
Cuts at: 2701 5130 2701
Size: 2429 3038
BspHI T'CATG_A
Cuts at: 3356 5086 3356
Size: 1730 3737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2158 2822 844
Size: 1314 664 3489
Fragments arranged by size:
3489 1314 664
BsrBI CCG'CTC
Cuts at: 1394 1823 3354 1394
Size: 429 1531 3507
Fragments arranged by size:
3507 1531 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2523 3962 4136 65
Size: 1183 1275 1439 174 1396
Fragments arranged by size:
1439 1396 1275 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4111 4298 1700
Size: 110 2301 187 2869
Fragments arranged by size:
2869 2301 187 110
BsrGI T'GTAC_A
Cuts at: 96 2125 2608 96
Size: 2029 483 2955
Fragments arranged by size:
2955 2029 483
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5467
BssSI C'ACGA_G
Cuts at: 3527 4539 3527
Size: 1012 4455
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5467
BstBI TT'CG_AA
Cuts at: 4271 4271
Size: 5467
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5467
BstYI r'GATC_y
Cuts at: 1972 2489 2797 3164 3549 3566 5018 5104 5116
Size: 517 308 367 385 17 1452 86 12
Cuts at: 5116 5462 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 517 385 367 346 308 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2259 513
Size: 552 939 255 3721
Fragments arranged by size:
3721 939 552 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4728
Fragments arranged by size:
4728 683 56
BtsI GCAGTG_nn'
Cuts at: 2983 3777 3797 2983
Size: 794 20 4653
Fragments arranged by size:
4653 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5467
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5432
DraI TTT'AAA
Cuts at: 2771 2771
Size: 5467
DraIII CAC_nnn'GTG
Cuts at: 2170 2170
Size: 5467
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4474 809
Size: 507 3158 1802
Fragments arranged by size:
3158 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2549 2914
Size: 54 1425 50 275 18 186 533 365
Cuts at: 2914 3803 5432 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 533 365 275 186 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2914 1537
Size: 275 1102 4090
Fragments arranged by size:
4090 1102 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3397 1353
Size: 2044 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4054 4428 4574 387
Size: 1064 138 2465 374 146 1280
Fragments arranged by size:
2465 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2460 2514 2606 3530 4127 4914 5182
Size: 393 828 54 92 924 597 787 268
Cuts at: 5182 1239
Size: 1524
Fragments arranged by size:
1524 924 828 787 597 393 268 92
54
EcoRI G'AATT_C
Cuts at: 2673 2776 3154 2673
Size: 103 378 4986
Fragments arranged by size:
4986 378 103
FspI TGC'GCA
Cuts at: 2060 3973 2060
Size: 1913 3554
HaeII r_GCGC'y
Cuts at: 1678 4614 1678
Size: 2936 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2723 2755 4108 4140 4182
Size: 32 135 32 1151 32 1353 32 42
Cuts at: 4182 4214 1373
Size: 32 2626
Fragments arranged by size:
2626 1353 1151 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2811 3057 669
Size: 988 281 873 246 3079
Fragments arranged by size:
3079 988 873 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5467
HpaI GTT'AAC
Cuts at: 3057 3057
Size: 5467
KpnI G_GTAC'C
Cuts at: 2792 2792
Size: 5467
MfeI C'AATT_G
Cuts at: 3066 3066
Size: 5467
MluI A'CGCG_T
Cuts at: 5343 5343
Size: 5467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4581 4765 1305
Size: 3276 184 2007
Fragments arranged by size:
3276 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3645
Fragments arranged by size:
3645 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3425 3784 3943 518
Size: 648 2259 359 159 2042
Fragments arranged by size:
2259 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2279 2614 2640 2883 3560 4708 4953 1606
Size: 673 335 26 243 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 673 335 245 243 26
NcoI C'CATG_G
Cuts at: 513 1065 2004 2259 513
Size: 552 939 255 3721
Fragments arranged by size:
3721 939 552 255
NdeI CA'TA_TG
Cuts at: 387 2076 387
Size: 1689 3778
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5467
NotI GC'GGCC_GC
Cuts at: 2914 2914
Size: 5467
NspI r_CATG'y
Cuts at: 1132 1229 2182 2686 2825 4370 1132
Size: 97 953 504 139 1545 2229
Fragments arranged by size:
2229 1545 953 504 139 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5467
PciI A'CATG_T
Cuts at: 2178 4366 2178
Size: 2188 3279
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5467
PmeI GTTT'AAAC
Cuts at: 2771 2771
Size: 5467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 5049 5081 3536
Size: 32 1481 32 3922
Fragments arranged by size:
3922 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5467
PsiI TTA'TAA
Cuts at: 3037 3037
Size: 5467
PstI C_TGCA'G
Cuts at: 830 2155 2284 2691 2819 830
Size: 1325 129 407 128 3478
Fragments arranged by size:
3478 1325 407 129 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5467
PvuII CAG'CTG
Cuts at: 2279 2614 2640 2279
Size: 335 26 5106
Fragments arranged by size:
5106 335 26
SalI G'TCGA_C
Cuts at: 1936 2809 1936
Size: 873 4594
SbfI CC_TGCA'GG
Cuts at: 2819 2819
Size: 5467
ScaI AGT'ACT
Cuts at: 3715 5427 3715
Size: 1712 3755
SfcI C'TryA_G
Cuts at: 826 1045 2151 2280 2687 2815 3950 4631 4822
Size: 219 1106 129 407 128 1135 681 191
Cuts at: 4822 826
Size: 1471
Fragments arranged by size:
1471 1135 1106 681 407 219 191 129
128
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5467
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5467
SmaI CCC'GGG
Cuts at: 1872 2794 1872
Size: 922 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 3571 4472 4734 820
Size: 197 926 303 1325 901 262 1553
Fragments arranged by size:
1553 1325 926 901 303 262 197
SnaBI TAC'GTA
Cuts at: 493 3258 493
Size: 2765 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5467
SphI G_CATG'C
Cuts at: 1132 2825 1132
Size: 1693 3774
SspI AAT'ATT
Cuts at: 5 52 3391 5
Size: 47 3339 2081
Fragments arranged by size:
3339 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5467
StyI C'CwwG_G
Cuts at: 513 1065 2004 2259 2906 513
Size: 552 939 255 647 3074
Fragments arranged by size:
3074 939 647 552 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2181 3494 3511 3664 3849 4294 325
Size: 1255 601 1313 17 153 185 445 1498
Fragments arranged by size:
1498 1313 1255 601 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2125 2608 3713
Size: 274 80 33 51 1443 148 483 1105
Cuts at: 3713 5425 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1105 483 274 148 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5427 5459 5427
Size: 32 5435
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5467
XbaI T'CTAG_A
Cuts at: 2803 2803
Size: 5467
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5467
XmaI C'CCGG_G
Cuts at: 1870 2792 1870
Size: 922 4545
XmnI GAAnn'nnTTC
Cuts at: 3596 3596
Size: 5467
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3275 276
Size: 53 83 186 936 1741 2468
Fragments arranged by size:
2468 1741 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI