Restriction map for
pFN21AB6989
(Circular) (Six-base) MAPSORT of: pFN21AB6989.seq Check: 4030 from: 1 to: 5362
pFN21AB6989 5362 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3172 278
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2705 1937
Size: 768 4594
Acc65I G'GTAC_C
Cuts at: 2683 2683
Size: 5362
AclI AA'CG_TT
Cuts at: 1563 3489 3862 1563
Size: 1926 373 3063
Fragments arranged by size:
3063 1926 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3425 4809 5077 1632
Size: 1793 1384 268 1917
Fragments arranged by size:
1917 1793 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5165
AflIII A'CryG_T
Cuts at: 1203 4261 5238 1203
Size: 3058 977 1327
Fragments arranged by size:
3058 1327 977
AgeI A'CCGG_T
Cuts at: 4193 4193
Size: 5362
AhdI GACnn_n'nnGTC
Cuts at: 2164 4091 2164
Size: 1927 3435
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2657 1184
Size: 1473 3889
AlwNI CAG_nnn'CTG
Cuts at: 2213 3105 4677 2213
Size: 892 1572 2898
Fragments arranged by size:
2898 1572 892
ApaLI G'TGCA_C
Cuts at: 2193 3419 4575 2193
Size: 1226 1156 2980
Fragments arranged by size:
2980 1226 1156
ApoI r'AATT_y
Cuts at: 1493 2648 2671 2897 3049 5158 1493
Size: 1155 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 1155 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 3916 160
Size: 872 2884 1606
Fragments arranged by size:
2884 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 2118 2507 2687 1870
Size: 73 97 78 389 180 4545
Fragments arranged by size:
4545 389 180 97 78 73
BamHI G'GATC_C
Cuts at: 2692 3059 2692
Size: 367 4995
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2683 4138 618
Size: 325 217 627 896 1455 1842
Fragments arranged by size:
1842 1455 896 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2259 2681 1391
Size: 868 422 4072
Fragments arranged by size:
4072 868 422
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3553 3587 1903
Size: 34 50 34 1532 34 3678
Fragments arranged by size:
3678 1532 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3254 4464 3254
Size: 1210 4152
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5362
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2237 2562 3973
Size: 107 122 71 631 605 565 325 1411
Cuts at: 3973 136
Size: 1525
Fragments arranged by size:
1525 1411 631 605 565 325 122 107
71
BglII A'GATC_T
Cuts at: 5357 5357
Size: 5362
BlpI GC'TnA_GC
Cuts at: 2779 2779
Size: 5362
Bme1580I G_kGCm'C
Cuts at: 1790 2197 3423 4579 1790
Size: 407 1226 1156 2573
Fragments arranged by size:
2573 1226 1156 407
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3093 4051 442
Size: 416 53 83 399 24 1676 958 1753
Fragments arranged by size:
1753 1676 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2294 4022 1239
Size: 1034 21 1728 2579
Fragments arranged by size:
2579 1728 1034 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2551 3487 4352 4650 2551
Size: 936 865 298 3263
Fragments arranged by size:
3263 936 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4025 882
Size: 617 2526 2219
Fragments arranged by size:
2526 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3153 493
Size: 711 1949 2702
Fragments arranged by size:
2702 1949 711
BsaBI GATnn'nnATC
Cuts at: 5356 5356
Size: 5362
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3169 3551 4186 275
Size: 53 83 186 936 1636 382 635 1451
Fragments arranged by size:
1636 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3795 4193 4467 4614 5025 3795
Size: 398 274 147 411 4132
Fragments arranged by size:
4132 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2379 2409 1178
Size: 30 165 30 976 30 4131
Fragments arranged by size:
4131 976 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 1186
Size: 1339 4023
BseYI C'CCAG_C
Cuts at: 2370 4565 5061 2370
Size: 2195 496 2671
Fragments arranged by size:
2671 2195 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 2334 2536 1163
Size: 573 423 175 202 3989
Fragments arranged by size:
3989 573 423 202 175
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2812 3573 4601 1540
Size: 275 186 811 761 1028 2301
Fragments arranged by size:
2301 1028 811 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2023 2197 2259 3423 3508 4579 2023
Size: 174 62 1164 85 1071 2806
Fragments arranged by size:
2806 1164 1071 174 85 62
BsmI GAATG_Cn'
Cuts at: 2421 2873 2966 2421
Size: 452 93 4817
Fragments arranged by size:
4817 452 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2197 2259 2681 3423 3508 4579
Size: 399 233 174 62 422 742 85 1071
Cuts at: 4579 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 422 399 233 174 85
62
BspEI T'CCGG_A
Cuts at: 5025 5025
Size: 5362
BspHI T'CATG_A
Cuts at: 3251 4981 3251
Size: 1730 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2717 844
Size: 1873 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3249 1394
Size: 429 1426 3507
Fragments arranged by size:
3507 1426 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2641 3857 4031 65
Size: 1183 1393 1216 174 1396
Fragments arranged by size:
1396 1393 1216 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2606 4006 4193 1700
Size: 110 796 1400 187 2869
Fragments arranged by size:
2869 1400 796 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5362
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5362
BssSI C'ACGA_G
Cuts at: 2177 3422 4434 2177
Size: 1245 1012 3105
Fragments arranged by size:
3105 1245 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5362
BstBI TT'CG_AA
Cuts at: 4166 4166
Size: 5362
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5362
BstXI CCAn_nnnn'nTGG
Cuts at: 2074 2074
Size: 5362
BstYI r'GATC_y
Cuts at: 1972 2692 3059 3444 3461 4913 4999 5011 5357
Size: 720 367 385 17 1452 86 12 346
Cuts at: 5357 1972
Size: 1977
Fragments arranged by size:
1977 1452 720 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2226 2260 513
Size: 552 1161 34 3615
Fragments arranged by size:
3615 1161 552 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4623
Fragments arranged by size:
4623 683 56
BtsI GCAGTG_nn'
Cuts at: 2085 2878 3672 3692 2085
Size: 793 794 20 3755
Fragments arranged by size:
3755 794 793 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5362
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2274 2309 529
Size: 35 1710 35 3582
Fragments arranged by size:
3582 1710 35 35
DraI TTT'AAA
Cuts at: 2666 2666
Size: 5362
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2621 4369 809
Size: 507 1305 1748 1802
Fragments arranged by size:
1802 1748 1305 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2238 2547
Size: 54 1425 50 275 18 399 9 309
Cuts at: 2547 2809 3698 5327 8
Size: 262 889 1629 43
Fragments arranged by size:
1629 1425 889 399 309 275 262 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2809 1537
Size: 275 997 4090
Fragments arranged by size:
4090 997 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3292 1353
Size: 1939 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3949 4323 4469 387
Size: 1064 138 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2257 2257
Size: 5362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2294 3425 4022 4809 5077 1239
Size: 393 641 21 1131 597 787 268 1524
Fragments arranged by size:
1524 1131 787 641 597 393 268 21
EcoRI G'AATT_C
Cuts at: 2671 3049 2671
Size: 378 4984
FspI TGC'GCA
Cuts at: 3868 3868
Size: 5362
HaeII r_GCGC'y
Cuts at: 1678 2524 4509 1678
Size: 846 1985 2531
Fragments arranged by size:
2531 1985 846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2150 2182 2330 2362 4003
Size: 32 135 32 578 32 148 32 1641
Cuts at: 4003 4035 4077 4109 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1641 578 148 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2706 2952 669
Size: 988 281 768 246 3079
Fragments arranged by size:
3079 988 768 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5362
HpaI GTT'AAC
Cuts at: 2952 2952
Size: 5362
KpnI G_GTAC'C
Cuts at: 2687 2687
Size: 5362
MfeI C'AATT_G
Cuts at: 2961 2961
Size: 5362
MluI A'CGCG_T
Cuts at: 5238 5238
Size: 5362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4476 4660 1305
Size: 3171 184 2007
Fragments arranged by size:
3171 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 10
Size: 54 1425 343 717 2823
Fragments arranged by size:
2823 1425 717 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2078 3320 3679 3838 518
Size: 648 906 6 1242 359 159 2042
Fragments arranged by size:
2042 1242 906 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2173 2210 2251 2603 2778 3455 4603 4848
Size: 567 37 41 352 175 677 1148 245
Cuts at: 4848 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 567 352 245 175 41
37
NcoI C'CATG_G
Cuts at: 513 1065 2260 513
Size: 552 1195 3615
Fragments arranged by size:
3615 1195 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5362
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5362
NotI GC'GGCC_GC
Cuts at: 2809 2809
Size: 5362
NspI r_CATG'y
Cuts at: 1132 1229 2503 2720 4265 1132
Size: 97 1274 217 1545 2229
Fragments arranged by size:
2229 1545 1274 217 97
PasI CC'CwG_GG
Cuts at: 1348 2407 1348
Size: 1059 4303
PciI A'CATG_T
Cuts at: 4261 4261
Size: 5362
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2227 1108
Size: 1119 4243
PmeI GTTT'AAAC
Cuts at: 2666 2666
Size: 5362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 4944 4976 3431
Size: 32 1481 32 3817
Fragments arranged by size:
3817 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5362
PsiI TTA'TAA
Cuts at: 2932 2932
Size: 5362
PstI C_TGCA'G
Cuts at: 830 2131 2714 830
Size: 1301 583 3478
Fragments arranged by size:
3478 1301 583
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5362
PvuII CAG'CTG
Cuts at: 2210 2251 2603 2210
Size: 41 352 4969
Fragments arranged by size:
4969 352 41
SacI G_AGCT'C
Cuts at: 2259 2259
Size: 5362
SalI G'TCGA_C
Cuts at: 1936 2704 1936
Size: 768 4594
SbfI CC_TGCA'GG
Cuts at: 2714 2714
Size: 5362
ScaI AGT'ACT
Cuts at: 3610 5322 3610
Size: 1712 3650
SexAI A'CCwGG_T
Cuts at: 2586 2586
Size: 5362
SfcI C'TryA_G
Cuts at: 826 1045 2127 2710 3845 4526 4717 826
Size: 219 1082 583 1135 681 191 1471
Fragments arranged by size:
1471 1135 1082 681 583 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2237 2237
Size: 5362
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5362
SmaI CCC'GGG
Cuts at: 1872 2042 2689 1872
Size: 170 647 4545
Fragments arranged by size:
4545 647 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2566 3466 4367 4629 820
Size: 197 926 623 900 901 262 1553
Fragments arranged by size:
1553 926 901 900 623 262 197
SnaBI TAC'GTA
Cuts at: 493 3153 493
Size: 2660 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5362
SphI G_CATG'C
Cuts at: 1132 2720 1132
Size: 1588 3774
SspI AAT'ATT
Cuts at: 5 52 3286 5
Size: 47 3234 2081
Fragments arranged by size:
3234 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5362
StyI C'CwwG_G
Cuts at: 513 1065 2260 2801 513
Size: 552 1195 541 3074
Fragments arranged by size:
3074 1195 552 541
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3389 3406 3559 3744 4189 325
Size: 1255 1809 17 153 185 445 1498
Fragments arranged by size:
1809 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3608 5320 96
Size: 274 80 33 51 1443 1631 1712 138
Fragments arranged by size:
1712 1631 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5322 5354 5322
Size: 32 5330
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5362
XbaI T'CTAG_A
Cuts at: 2698 2698
Size: 5362
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2077 2077
Size: 5362
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5362
XmaI C'CCGG_G
Cuts at: 1870 2040 2687 1870
Size: 170 647 4545
Fragments arranged by size:
4545 647 170
XmnI GAAnn'nnTTC
Cuts at: 3491 3491
Size: 5362
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3170 276
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SfoI SrfI SwaI