Restriction map for
pFN21AB6986
(Circular) (Six-base) MAPSORT of: pFN21AB6986.seq Check: 7181 from: 1 to: 5449
pFN21AB6986 5449 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3259 278
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2792 1937
Size: 855 4594
Acc65I G'GTAC_C
Cuts at: 2770 2770
Size: 5449
AclI AA'CG_TT
Cuts at: 1563 3576 3949 1563
Size: 2013 373 3063
Fragments arranged by size:
3063 2013 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2366 2691 3512 4896 5164 1632
Size: 734 325 821 1384 268 1917
Fragments arranged by size:
1917 1384 821 734 325 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5252
AflIII A'CryG_T
Cuts at: 1203 4348 5325 1203
Size: 3145 977 1327
Fragments arranged by size:
3145 1327 977
AgeI A'CCGG_T
Cuts at: 4280 4280
Size: 5449
AhdI GACnn_n'nnGTC
Cuts at: 4178 4178
Size: 5449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5449
AlwNI CAG_nnn'CTG
Cuts at: 3192 4764 3192
Size: 1572 3877
ApaI G_GGCC'C
Cuts at: 2266 2266
Size: 5449
ApaLI G'TGCA_C
Cuts at: 3506 4662 3506
Size: 1156 4293
ApoI r'AATT_y
Cuts at: 1493 2758 2984 3136 5245 1493
Size: 1265 226 152 2109 1697
Fragments arranged by size:
2109 1697 1265 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4003 160
Size: 872 2971 1606
Fragments arranged by size:
2971 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
AvaI C'yCGr_G
Cuts at: 1870 1943 2265 2452 2774 1870
Size: 73 322 187 322 4545
Fragments arranged by size:
4545 322 322 187 73
AvrII C'CTAG_G
Cuts at: 2429 2429
Size: 5449
BamHI G'GATC_C
Cuts at: 2779 3146 2779
Size: 367 5082
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 2770 4225 618
Size: 325 217 627 708 275 1455 1842
Fragments arranged by size:
1842 1455 708 627 325 275 217
BanII G_rGCy'C
Cuts at: 1391 2093 2266 2768 1391
Size: 702 173 502 4072
Fragments arranged by size:
4072 702 502 173
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 928
Size: 971 124 4354
Fragments arranged by size:
4354 971 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3640 3674 1903
Size: 34 1703 34 3678
Fragments arranged by size:
3678 1703 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3341 4551 3341
Size: 1210 4239
BclI T'GATC_A
Cuts at: 1548 2577 1548
Size: 1029 4420
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2474 4060 136
Size: 107 122 71 631 605 802 1586 1525
Fragments arranged by size:
1586 1525 802 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2187 5444 2187
Size: 3257 2192
BlpI GC'TnA_GC
Cuts at: 2866 2866
Size: 5449
Bme1580I G_kGCm'C
Cuts at: 1790 2266 2459 3510 4666 1790
Size: 476 193 1051 1156 2573
Fragments arranged by size:
2573 1156 1051 476 193
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5449
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3180 4138 442
Size: 416 53 83 399 24 1763 958 1753
Fragments arranged by size:
1763 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2540 4109 1239
Size: 1301 1569 2579
Fragments arranged by size:
2579 1569 1301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 3574 4439 4737 2639
Size: 935 865 298 3351
Fragments arranged by size:
3351 935 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4112 882
Size: 617 2613 2219
Fragments arranged by size:
2613 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3240 493
Size: 711 2036 2702
Fragments arranged by size:
2702 2036 711
BsaBI GATnn'nnATC
Cuts at: 2252 5443 2252
Size: 3191 2258
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3256 3638 4273 275
Size: 53 83 186 936 1723 382 635 1451
Fragments arranged by size:
1723 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3882 4280 4554 4701 5112 3882
Size: 398 274 147 411 4219
Fragments arranged by size:
4219 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2132 2162 1178
Size: 30 165 30 729 30 4465
Fragments arranged by size:
4465 729 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5449
BseYI C'CCAG_C
Cuts at: 4652 5148 4652
Size: 496 4953
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4876
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2017 2097 2899 3660 4688 1540
Size: 275 186 16 80 802 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 275 186 80 16
BsiHKAI G_wGCw'C
Cuts at: 3510 3595 4666 3510
Size: 85 1071 4293
Fragments arranged by size:
4293 1071 85
BsmI GAATG_Cn'
Cuts at: 2960 3053 2960
Size: 93 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2266 2459 2768 3510 3595 4666
Size: 399 303 173 193 309 742 85 1071
Cuts at: 4666 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 309 303 193 173
85
BspEI T'CCGG_A
Cuts at: 5112 5112
Size: 5449
BspHI T'CATG_A
Cuts at: 2634 3338 5068 2634
Size: 704 1730 3015
Fragments arranged by size:
3015 1730 704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 844
Size: 1960 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3336 1394
Size: 429 1513 3507
Fragments arranged by size:
3507 1513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2374 3944 4118 65
Size: 1183 1126 1570 174 1396
Fragments arranged by size:
1570 1396 1183 1126 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2286 4093 4280 1700
Size: 110 476 1807 187 2869
Fragments arranged by size:
2869 1807 476 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5449
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5449
BssSI C'ACGA_G
Cuts at: 3509 4521 3509
Size: 1012 4437
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5449
BstBI TT'CG_AA
Cuts at: 4253 4253
Size: 5449
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5449
BstXI CCAn_nnnn'nTGG
Cuts at: 2572 2572
Size: 5449
BstYI r'GATC_y
Cuts at: 1972 2187 2779 3146 3531 3548 5000 5086 5098
Size: 215 592 367 385 17 1452 86 12
Cuts at: 5098 5444 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 592 385 367 346 215 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2097 513
Size: 552 1032 3865
Fragments arranged by size:
3865 1032 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2320 487
Size: 683 56 1094 3616
Fragments arranged by size:
3616 1094 683 56
BtsI GCAGTG_nn'
Cuts at: 2965 3759 3779 2965
Size: 794 20 4635
Fragments arranged by size:
4635 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5449
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5414
DraI TTT'AAA
Cuts at: 2664 2753 2664
Size: 89 5360
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4456 809
Size: 507 3140 1802
Fragments arranged by size:
3140 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2154 2896
Size: 54 1425 50 275 18 214 110 742
Cuts at: 2896 3785 5414 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 742 275 214 110 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2896 1537
Size: 275 1084 4090
Fragments arranged by size:
4090 1084 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2057 2110 3379 1353
Size: 704 53 1269 3423
Fragments arranged by size:
3423 1269 704 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4036 4410 4556 387
Size: 1064 138 2447 374 146 1280
Fragments arranged by size:
2447 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 2540 2691 3512 4109 4896 5164
Size: 393 734 174 151 821 597 787 268
Cuts at: 5164 1239
Size: 1524
Fragments arranged by size:
1524 821 787 734 597 393 268 174
151
EcoO109I rG'GnC_Cy
Cuts at: 2262 2302 2262
Size: 40 5409
EcoRI G'AATT_C
Cuts at: 2758 3136 2758
Size: 378 5071
FspI TGC'GCA
Cuts at: 3955 3955
Size: 5449
HaeII r_GCGC'y
Cuts at: 1678 4596 1678
Size: 2918 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2629 2650 2661
Size: 32 135 32 560 32 465 21 11
Cuts at: 2661 2682 4090 4122 4164 4196 1373
Size: 21 1408 32 42 32 2626
Fragments arranged by size:
2626 1408 560 465 135 42 32 32
32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2793 3039 669
Size: 988 281 855 246 3079
Fragments arranged by size:
3079 988 855 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5449
HpaI GTT'AAC
Cuts at: 3039 3039
Size: 5449
KpnI G_GTAC'C
Cuts at: 2774 2774
Size: 5449
MfeI C'AATT_G
Cuts at: 3048 3048
Size: 5449
MluI A'CGCG_T
Cuts at: 5325 5325
Size: 5449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4563 4747 1305
Size: 3258 184 2007
Fragments arranged by size:
3258 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2156 10
Size: 54 1425 343 324 3303
Fragments arranged by size:
3303 1425 343 324 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3407 3766 3925 518
Size: 648 2241 359 159 2042
Fragments arranged by size:
2241 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2099 2865 3542 4690 4935 1606
Size: 493 766 677 1148 245 2120
Fragments arranged by size:
2120 1148 766 677 493 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4897
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5449
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5449
NotI GC'GGCC_GC
Cuts at: 2896 2896
Size: 5449
NspI r_CATG'y
Cuts at: 1132 1229 2807 4352 1132
Size: 97 1578 1545 2229
Fragments arranged by size:
2229 1578 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5449
PciI A'CATG_T
Cuts at: 4348 4348
Size: 5449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5449
PfoI T'CCnGG_A
Cuts at: 2517 2589 2517
Size: 72 5377
PmeI GTTT'AAAC
Cuts at: 2753 2753
Size: 5449
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 5031 5063 3518
Size: 32 1481 32 3904
Fragments arranged by size:
3904 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 2302
Size: 5449
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5449
PsiI TTA'TAA
Cuts at: 3019 3019
Size: 5449
PspOMI G'GGCC_C
Cuts at: 2262 2262
Size: 5449
PstI C_TGCA'G
Cuts at: 830 2166 2801 830
Size: 1336 635 3478
Fragments arranged by size:
3478 1336 635
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5449
SacII CC_GC'GG
Cuts at: 2100 2100
Size: 5449
SalI G'TCGA_C
Cuts at: 1936 2791 1936
Size: 855 4594
SbfI CC_TGCA'GG
Cuts at: 2801 2801
Size: 5449
ScaI AGT'ACT
Cuts at: 3697 5409 3697
Size: 1712 3737
SfcI C'TryA_G
Cuts at: 826 1045 2162 2797 3932 4613 4804 826
Size: 219 1117 635 1135 681 191 1471
Fragments arranged by size:
1471 1135 1117 681 635 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5449
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5449
SmaI CCC'GGG
Cuts at: 1872 2267 2776 1872
Size: 395 509 4545
Fragments arranged by size:
4545 509 395
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 3553 4454 4716 820
Size: 197 926 711 899 901 262 1553
Fragments arranged by size:
1553 926 901 899 711 262 197
SnaBI TAC'GTA
Cuts at: 493 3240 493
Size: 2747 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5449
SphI G_CATG'C
Cuts at: 1132 2807 1132
Size: 1675 3774
SspI AAT'ATT
Cuts at: 5 52 3373 5
Size: 47 3321 2081
Fragments arranged by size:
3321 2081 47
StuI AGG'CCT
Cuts at: 1517 2229 1517
Size: 712 4737
StyI C'CwwG_G
Cuts at: 513 1065 2429 2888 513
Size: 552 1364 459 3074
Fragments arranged by size:
3074 1364 552 459
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3476 3493 3646 3831 4276 325
Size: 1255 1896 17 153 185 445 1498
Fragments arranged by size:
1896 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 3695 5407
Size: 274 80 33 51 1443 342 1376 1712
Cuts at: 5407 96
Size: 138
Fragments arranged by size:
1712 1443 1376 342 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2176 2208 5409 5441 2176
Size: 32 3201 32 2184
Fragments arranged by size:
3201 2184 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2207 2306 1316
Size: 891 99 4459
Fragments arranged by size:
4459 891 99
XbaI T'CTAG_A
Cuts at: 2785 2785
Size: 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5449
XmaI C'CCGG_G
Cuts at: 1870 2265 2774 1870
Size: 395 509 4545
Fragments arranged by size:
4545 509 395
XmnI GAAnn'nnTTC
Cuts at: 2598 3578 2598
Size: 980 4469
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3257 276
Size: 53 83 186 936 1723 2468
Fragments arranged by size:
2468 1723 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI