Restriction map for
pFN21AB6983
(Circular) (Six-base) MAPSORT of: pFN21AB6983.seq Check: 2783 from: 1 to: 5359
pFN21AB6983 5359 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3169 278
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2702 1937
Size: 765 4594
Acc65I G'GTAC_C
Cuts at: 2680 2680
Size: 5359
AclI AA'CG_TT
Cuts at: 1563 3486 3859 1563
Size: 1923 373 3063
Fragments arranged by size:
3063 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3422 4806 5074 1632
Size: 1790 1384 268 1917
Fragments arranged by size:
1917 1790 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5162
AflIII A'CryG_T
Cuts at: 1203 4258 5235 1203
Size: 3055 977 1327
Fragments arranged by size:
3055 1327 977
AgeI A'CCGG_T
Cuts at: 4190 4190
Size: 5359
AhdI GACnn_n'nnGTC
Cuts at: 4088 4088
Size: 5359
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5359
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5359
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2580 2612 2580
Size: 32 5327
AlwNI CAG_nnn'CTG
Cuts at: 2383 3102 4674 2383
Size: 719 1572 3068
Fragments arranged by size:
3068 1572 719
ApaI G_GGCC'C
Cuts at: 2067 2067
Size: 5359
ApaLI G'TGCA_C
Cuts at: 3416 4572 3416
Size: 1156 4203
ApoI r'AATT_y
Cuts at: 1493 2668 2894 3046 5155 1493
Size: 1175 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2334 3913 160
Size: 872 1302 1579 1606
Fragments arranged by size:
1606 1579 1302 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 2066 2684 1870
Size: 73 103 20 618 4545
Fragments arranged by size:
4545 618 103 73 20
BamHI G'GATC_C
Cuts at: 2689 3056 2689
Size: 367 4992
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2486 2680 4135 618
Size: 325 217 627 699 194 1455 1842
Fragments arranged by size:
1842 1455 699 627 325 217 194
BanII G_rGCy'C
Cuts at: 1391 2067 2598 2609 2678 1391
Size: 676 531 11 69 4072
Fragments arranged by size:
4072 676 531 69 11
BbeI G_GCGC'C
Cuts at: 2490 2490
Size: 5359
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3550 3584 1903
Size: 34 1613 34 3678
Fragments arranged by size:
3678 1613 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3251 4461 3251
Size: 1210 4149
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3970 136
Size: 107 122 71 631 605 2298 1525
Fragments arranged by size:
2298 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2208 2437 5354 2208
Size: 229 2917 2213
Fragments arranged by size:
2917 2213 229
BlpI GC'TnA_GC
Cuts at: 2776 2776
Size: 5359
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2620 3420 4576 1790
Size: 277 553 800 1156 2573
Fragments arranged by size:
2573 1156 800 553 277
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5359
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2607 3090 4048
Size: 416 53 83 399 24 1190 483 958
Cuts at: 4048 442
Size: 1753
Fragments arranged by size:
1753 1190 958 483 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 4019 1239
Size: 1223 1557 2579
Fragments arranged by size:
2579 1557 1223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3484 4349 4647 3484
Size: 865 298 4196
Fragments arranged by size:
4196 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4022 882
Size: 617 2523 2219
Fragments arranged by size:
2523 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2500 3150 493
Size: 711 1296 650 2702
Fragments arranged by size:
2702 1296 711 650
BsaBI GATnn'nnATC
Cuts at: 5353 5353
Size: 5359
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 2487 3166 3548
Size: 53 83 186 936 488 466 679 382
Cuts at: 3548 4183 275
Size: 635 1451
Fragments arranged by size:
1451 936 679 635 488 466 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2574 3792 4190 4464 4611 5022 2574
Size: 1218 398 274 147 411 2911
Fragments arranged by size:
2911 1218 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2580 2610 1178
Size: 30 165 30 1177 30 3927
Fragments arranged by size:
3927 1177 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5359
BseYI C'CCAG_C
Cuts at: 2081 2230 4562 5058 2081
Size: 149 2332 496 2382
Fragments arranged by size:
2382 2332 496 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4786
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2809 3570 4598 1540
Size: 275 186 808 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2598 3420 3505 4576 2598
Size: 822 85 1071 3381
Fragments arranged by size:
3381 1071 822 85
BsmI GAATG_Cn'
Cuts at: 2870 2963 2870
Size: 93 5266
BsmBI CGTCTCn'nnnn_
Cuts at: 2537 2537
Size: 5359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2598 2609 2620 2678 3420 3505
Size: 399 277 531 11 11 58 742 85
Cuts at: 3505 4576 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 531 399 277 85 58
11 11
BspEI T'CCGG_A
Cuts at: 2574 5022 2574
Size: 2448 2911
BspHI T'CATG_A
Cuts at: 3248 4978 3248
Size: 1730 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2714 844
Size: 1870 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3246 1394
Size: 429 1423 3507
Fragments arranged by size:
3507 1423 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 4028 65
Size: 1183 2606 174 1396
Fragments arranged by size:
2606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2340 4003 4190 1700
Size: 110 530 1663 187 2869
Fragments arranged by size:
2869 1663 530 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5359
BssHII G'CGCG_C
Cuts at: 1925 2133 1925
Size: 208 5151
BssSI C'ACGA_G
Cuts at: 3419 4431 3419
Size: 1012 4347
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2296 2383 2563 794
Size: 1502 87 180 3590
Fragments arranged by size:
3590 1502 180 87
BstBI TT'CG_AA
Cuts at: 4163 4163
Size: 5359
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5359
BstYI r'GATC_y
Cuts at: 1972 2208 2437 2689 3056 3441 3458 4910 4996
Size: 236 229 252 367 385 17 1452 86
Cuts at: 4996 5008 5354 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 252 236 229
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2256 2308 513
Size: 552 1191 52 3564
Fragments arranged by size:
3564 1191 552 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4620
Fragments arranged by size:
4620 683 56
BtsI GCAGTG_nn'
Cuts at: 2221 2875 3669 3689 2221
Size: 654 794 20 3891
Fragments arranged by size:
3891 794 654 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5324
DraI TTT'AAA
Cuts at: 2663 2663
Size: 5359
DraIII CAC_nnn'GTG
Cuts at: 2500 2500
Size: 5359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4366 809
Size: 507 3050 1802
Fragments arranged by size:
3050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2305 2520 2806
Size: 54 1425 50 275 18 475 215 286
Cuts at: 2806 3695 5324 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 475 286 275 215 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2806 1537
Size: 275 994 4090
Fragments arranged by size:
4090 994 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 2375 3289 1353
Size: 909 113 914 3423
Fragments arranged by size:
3423 914 909 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3946 4320 4466 387
Size: 1064 138 2357 374 146 1280
Fragments arranged by size:
2357 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2596 2596
Size: 5359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 3422 4019 4806 5074 1239
Size: 393 830 960 597 787 268 1524
Fragments arranged by size:
1524 960 830 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2057 2071 2458 2057
Size: 14 387 4958
Fragments arranged by size:
4958 387 14
EcoRI G'AATT_C
Cuts at: 2668 3046 2668
Size: 378 4981
FspI TGC'GCA
Cuts at: 3865 3865
Size: 5359
HaeII r_GCGC'y
Cuts at: 1678 2455 2490 4506 1678
Size: 777 35 2016 2531
Fragments arranged by size:
2531 2016 777 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 4000 4032 4074
Size: 32 135 32 857 32 1539 32 42
Cuts at: 4074 4106 1373
Size: 32 2626
Fragments arranged by size:
2626 1539 857 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 2949 669
Size: 988 281 765 246 3079
Fragments arranged by size:
3079 988 765 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5359
HpaI GTT'AAC
Cuts at: 2949 2949
Size: 5359
KasI G'GCGC_C
Cuts at: 2486 2486
Size: 5359
KpnI G_GTAC'C
Cuts at: 2684 2684
Size: 5359
MfeI C'AATT_G
Cuts at: 2958 2958
Size: 5359
MluI A'CGCG_T
Cuts at: 5235 5235
Size: 5359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4473 4657 1305
Size: 3168 184 2007
Fragments arranged by size:
3168 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2307 10
Size: 54 1425 343 475 3062
Fragments arranged by size:
3062 1425 475 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3317 3676 3835 518
Size: 648 2151 359 159 2042
Fragments arranged by size:
2151 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2258 2560 2775 3452 4600 4845 1606
Size: 407 245 302 215 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 407 302 245 245 215
NarI GG'CG_CC
Cuts at: 2487 2487
Size: 5359
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4807
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5359
NotI GC'GGCC_GC
Cuts at: 2806 2806
Size: 5359
NspI r_CATG'y
Cuts at: 1132 1229 2299 2717 4262 1132
Size: 97 1070 418 1545 2229
Fragments arranged by size:
2229 1545 1070 418 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5359
PciI A'CATG_T
Cuts at: 4258 4258
Size: 5359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2500 1108
Size: 1392 3967
PfoI T'CCnGG_A
Cuts at: 2024 2247 2024
Size: 223 5136
PmeI GTTT'AAAC
Cuts at: 2663 2663
Size: 5359
PmlI CAC'GTG
Cuts at: 2500 2500
Size: 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2580 2612 3428 3460 4941 4973 2580
Size: 32 816 32 1481 32 2966
Fragments arranged by size:
2966 1481 816 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2057 2071 2458 2057
Size: 14 387 4958
Fragments arranged by size:
4958 387 14
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5359
PsiI TTA'TAA
Cuts at: 2929 2929
Size: 5359
PspOMI G'GGCC_C
Cuts at: 2063 2063
Size: 5359
PstI C_TGCA'G
Cuts at: 830 2216 2711 830
Size: 1386 495 3478
Fragments arranged by size:
3478 1386 495
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5359
PvuII CAG'CTG
Cuts at: 2560 2560
Size: 5359
SacI G_AGCT'C
Cuts at: 2598 2598
Size: 5359
SacII CC_GC'GG
Cuts at: 2259 2259
Size: 5359
SalI G'TCGA_C
Cuts at: 1936 2701 1936
Size: 765 4594
SanDI GG'GwC_CC
Cuts at: 2071 2071
Size: 5359
SapI GCTCTTCn'nnn_
Cuts at: 2375 2375
Size: 5359
SbfI CC_TGCA'GG
Cuts at: 2711 2711
Size: 5359
ScaI AGT'ACT
Cuts at: 3607 5319 3607
Size: 1712 3647
SfcI C'TryA_G
Cuts at: 826 1045 2212 2707 3842 4523 4714 826
Size: 219 1167 495 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 681 495 219 191
SfoI GGC'GCC
Cuts at: 2488 2488
Size: 5359
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5359
SmaI CCC'GGG
Cuts at: 1872 2048 2068 2686 1872
Size: 176 20 618 4545
Fragments arranged by size:
4545 618 176 20
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3463 4364 4626 820
Size: 197 926 1520 901 262 1553
Fragments arranged by size:
1553 1520 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3150 493
Size: 2657 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5359
SphI G_CATG'C
Cuts at: 1132 2717 1132
Size: 1585 3774
SspI AAT'ATT
Cuts at: 5 52 3283 5
Size: 47 3231 2081
Fragments arranged by size:
3231 2081 47
StuI AGG'CCT
Cuts at: 1517 2516 1517
Size: 999 4360
StyI C'CwwG_G
Cuts at: 513 1065 2798 513
Size: 552 1733 3074
Fragments arranged by size:
3074 1733 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 3403 3556 3741 4186 325
Size: 1255 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 3605 5317
Size: 274 80 33 51 1443 315 1313 1712
Cuts at: 5317 96
Size: 138
Fragments arranged by size:
1712 1443 1313 315 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5319 5351 5319
Size: 32 5327
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5359
XbaI T'CTAG_A
Cuts at: 2695 2695
Size: 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5359
XmaI C'CCGG_G
Cuts at: 1870 2046 2066 2684 1870
Size: 176 20 618 4545
Fragments arranged by size:
4545 618 176 20
XmnI GAAnn'nnTTC
Cuts at: 3488 3488
Size: 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3167 276
Size: 53 83 186 936 1633 2468
Fragments arranged by size:
2468 1633 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI
PsrI RsrII SexAI SfiI SrfI SwaI XcmI