Restriction map for
pFN21AB6973
(Circular) (Six-base) MAPSORT of: pFN21AB6973.seq Check: 4509 from: 1 to: 5356
pFN21AB6973 5356 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2348 2348
Size: 5356
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3166 278
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2699 1937
Size: 762 4594
Acc65I G'GTAC_C
Cuts at: 2677 2677
Size: 5356
AclI AA'CG_TT
Cuts at: 1563 3483 3856 1563
Size: 1920 373 3063
Fragments arranged by size:
3063 1920 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3419 4803 5071 1632
Size: 1787 1384 268 1917
Fragments arranged by size:
1917 1787 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5159
AflIII A'CryG_T
Cuts at: 1203 4255 5232 1203
Size: 3052 977 1327
Fragments arranged by size:
3052 1327 977
AgeI A'CCGG_T
Cuts at: 4187 4187
Size: 5356
AhdI GACnn_n'nnGTC
Cuts at: 4085 4085
Size: 5356
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5356
AlwNI CAG_nnn'CTG
Cuts at: 2218 3099 4671 2218
Size: 881 1572 2903
Fragments arranged by size:
2903 1572 881
ApaI G_GGCC'C
Cuts at: 2541 2541
Size: 5356
ApaLI G'TGCA_C
Cuts at: 3413 4569 3413
Size: 1156 4200
ApoI r'AATT_y
Cuts at: 1493 2191 2665 2891 3043 5152 1493
Size: 698 474 226 152 2109 1697
Fragments arranged by size:
2109 1697 698 474 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3910 160
Size: 872 2878 1606
Fragments arranged by size:
2878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 2579 2681 1870
Size: 73 474 162 102 4545
Fragments arranged by size:
4545 474 162 102 73
BamHI G'GATC_C
Cuts at: 2686 3053 2686
Size: 367 4989
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2638 2677 4132 618
Size: 325 217 627 851 39 1455 1842
Fragments arranged by size:
1842 1455 851 627 325 217 39
BanII G_rGCy'C
Cuts at: 1391 2541 2675 1391
Size: 1150 134 4072
Fragments arranged by size:
4072 1150 134
BbeI G_GCGC'C
Cuts at: 2642 2642
Size: 5356
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3547 3581 1903
Size: 34 1610 34 3678
Fragments arranged by size:
3678 1610 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3248 4458 3248
Size: 1210 4146
BclI T'GATC_A
Cuts at: 1548 2091 2304 1548
Size: 543 213 4600
Fragments arranged by size:
4600 543 213
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2349 2450 3967
Size: 107 122 71 631 605 677 101 1517
Cuts at: 3967 136
Size: 1525
Fragments arranged by size:
1525 1517 677 631 605 122 107 101
71
BglII A'GATC_T
Cuts at: 2370 5351 2370
Size: 2981 2375
BlpI GC'TnA_GC
Cuts at: 2462 2773 2462
Size: 311 5045
Bme1580I G_kGCm'C
Cuts at: 1790 2541 3417 4573 1790
Size: 751 876 1156 2573
Fragments arranged by size:
2573 1156 876 751
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5356
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2300 3087 4045
Size: 416 53 83 399 24 883 787 958
Cuts at: 4045 442
Size: 1753
Fragments arranged by size:
1753 958 883 787 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2225 2411 4016 1239
Size: 863 123 186 1605 2579
Fragments arranged by size:
2579 1605 863 186 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3481 4346 4644 3481
Size: 865 298 4193
Fragments arranged by size:
4193 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4019 882
Size: 617 2520 2219
Fragments arranged by size:
2520 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3147 493
Size: 711 1943 2702
Fragments arranged by size:
2702 1943 711
BsaBI GATnn'nnATC
Cuts at: 5350 5350
Size: 5356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2486 2639 3163 3545
Size: 53 83 186 936 953 153 524 382
Cuts at: 3545 4180 275
Size: 635 1451
Fragments arranged by size:
1451 953 936 635 524 382 186 153
83 53
BsaWI w'CCGG_w
Cuts at: 3789 4187 4461 4608 5019 3789
Size: 398 274 147 411 4126
Fragments arranged by size:
4126 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5131
Fragments arranged by size:
5131 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2540 1186
Size: 1354 4002
BseYI C'CCAG_C
Cuts at: 2014 2549 4559 5055 2014
Size: 535 2010 496 2315
Fragments arranged by size:
2315 2010 535 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2359 1163
Size: 573 623 4160
Fragments arranged by size:
4160 623 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2628 2806 3567 4595 1540
Size: 275 186 627 178 761 1028 2301
Fragments arranged by size:
2301 1028 761 627 275 186 178
BsiHKAI G_wGCw'C
Cuts at: 3417 3502 4573 3417
Size: 85 1071 4200
Fragments arranged by size:
4200 1071 85
BsmI GAATG_Cn'
Cuts at: 2867 2960 2867
Size: 93 5263
BsmBI CGTCTCn'nnnn_
Cuts at: 2477 2477
Size: 5356
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2541 2675 3417 3502 4573 1391
Size: 399 751 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 751 742 399 134 85
BspEI T'CCGG_A
Cuts at: 5019 5019
Size: 5356
BspHI T'CATG_A
Cuts at: 3245 4975 3245
Size: 1730 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2348 2644 2711 844
Size: 1504 296 67 3489
Fragments arranged by size:
3489 1504 296 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3243 1394
Size: 429 1420 3507
Fragments arranged by size:
3507 1420 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3851 4025 65
Size: 1183 2603 174 1396
Fragments arranged by size:
2603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2488 4000 4187 1700
Size: 110 678 1512 187 2869
Fragments arranged by size:
2869 1512 678 187 110
BsrGI T'GTAC_A
Cuts at: 96 2310 96
Size: 2214 3142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5356
BssSI C'ACGA_G
Cuts at: 3416 4428 3416
Size: 1012 4344
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5356
BstBI TT'CG_AA
Cuts at: 4160 4160
Size: 5356
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5356
BstYI r'GATC_y
Cuts at: 1972 2370 2686 3053 3438 3455 4907 4993 5005
Size: 398 316 367 385 17 1452 86 12
Cuts at: 5005 5351 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 398 385 367 346 316 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 2280 513
Size: 552 939 69 207 3589
Fragments arranged by size:
3589 939 552 207 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4617
Fragments arranged by size:
4617 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 3666 3686 2872
Size: 794 20 4542
Fragments arranged by size:
4542 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5321
DraI TTT'AAA
Cuts at: 2660 2660
Size: 5356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2141 4363 809
Size: 507 825 2222 1802
Fragments arranged by size:
2222 1802 825 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2442 2565 2592
Size: 54 1425 50 275 18 612 123 27
Cuts at: 2592 2625 2803 3692 5321 8
Size: 33 178 889 1629 43
Fragments arranged by size:
1629 1425 889 612 275 178 123 54
50 43 33 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2625 2803 1537
Size: 275 813 178 4090
Fragments arranged by size:
4090 813 275 178
EarI CTCTTCn'nnn_
Cuts at: 1353 3286 1353
Size: 1933 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2615 3943 4317 4463 387
Size: 1064 138 1026 1328 374 146 1280
Fragments arranged by size:
1328 1280 1064 1026 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2225 2411 3419 4016 4803 5071
Size: 393 470 123 186 1008 597 787 268
Cuts at: 5071 1239
Size: 1524
Fragments arranged by size:
1524 1008 787 597 470 393 268 186
123
EcoO109I rG'GnC_Cy
Cuts at: 2451 2537 2451
Size: 86 5270
EcoRI G'AATT_C
Cuts at: 2665 3043 2665
Size: 378 4978
FspI TGC'GCA
Cuts at: 3862 3862
Size: 5356
HaeII r_GCGC'y
Cuts at: 1678 2288 2425 2642 4503 1678
Size: 610 137 217 1861 2531
Fragments arranged by size:
2531 1861 610 217 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373
Size: 32 135 32 2425 32 42 32 2626
Fragments arranged by size:
2626 2425 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2700 2946 669
Size: 988 281 762 246 3079
Fragments arranged by size:
3079 988 762 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5356
HpaI GTT'AAC
Cuts at: 2946 2946
Size: 5356
KasI G'GCGC_C
Cuts at: 2638 2638
Size: 5356
KpnI G_GTAC'C
Cuts at: 2681 2681
Size: 5356
MfeI C'AATT_G
Cuts at: 2955 2955
Size: 5356
MluI A'CGCG_T
Cuts at: 5232 5232
Size: 5356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4470 4654 1305
Size: 3165 184 2007
Fragments arranged by size:
3165 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3534
Fragments arranged by size:
3534 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 3314 3673 3832 518
Size: 648 1242 906 359 159 2042
Fragments arranged by size:
2042 1242 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2383 2461 2772 3449 4597 4842 1606
Size: 564 213 78 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 564 311 245 213 78
NaeI GCC'GGC
Cuts at: 2490 2490
Size: 5356
NarI GG'CG_CC
Cuts at: 2639 2639
Size: 5356
NcoI C'CATG_G
Cuts at: 513 1065 2004 2073 2280 513
Size: 552 939 69 207 3589
Fragments arranged by size:
3589 939 552 207 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5356
NgoMIV G'CCGG_C
Cuts at: 2488 2488
Size: 5356
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5356
NotI GC'GGCC_GC
Cuts at: 2803 2803
Size: 5356
NspI r_CATG'y
Cuts at: 1132 1229 2714 4259 1132
Size: 97 1485 1545 2229
Fragments arranged by size:
2229 1545 1485 97
PasI CC'CwG_GG
Cuts at: 1348 2051 1348
Size: 703 4653
PciI A'CATG_T
Cuts at: 4255 4255
Size: 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5356
PmeI GTTT'AAAC
Cuts at: 2660 2660
Size: 5356
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3425 3457 4938 4970 3425
Size: 32 1481 32 3811
Fragments arranged by size:
3811 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5356
PsiI TTA'TAA
Cuts at: 2926 2926
Size: 5356
PspOMI G'GGCC_C
Cuts at: 2537 2537
Size: 5356
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2318 2350 2318
Size: 32 5324
PstI C_TGCA'G
Cuts at: 830 2024 2228 2708 830
Size: 1194 204 480 3478
Fragments arranged by size:
3478 1194 480 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5356
PvuII CAG'CTG
Cuts at: 2170 2170
Size: 5356
SalI G'TCGA_C
Cuts at: 1936 2698 1936
Size: 762 4594
SbfI CC_TGCA'GG
Cuts at: 2708 2708
Size: 5356
ScaI AGT'ACT
Cuts at: 3604 5316 3604
Size: 1712 3644
SexAI A'CCwGG_T
Cuts at: 2136 2136
Size: 5356
SfcI C'TryA_G
Cuts at: 826 1045 2020 2224 2704 3839 4520 4711 826
Size: 219 975 204 480 1135 681 191 1471
Fragments arranged by size:
1471 1135 975 681 480 219 204 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2450 2450
Size: 5356
SfoI GGC'GCC
Cuts at: 2640 2640
Size: 5356
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
SgrAI Cr'CCGG_yG
Cuts at: 1700 2488 1700
Size: 788 4568
SmaI CCC'GGG
Cuts at: 1872 2581 2683 1872
Size: 709 102 4545
Fragments arranged by size:
4545 709 102
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3460 4361 4623 820
Size: 197 926 1517 901 262 1553
Fragments arranged by size:
1553 1517 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3147 493
Size: 2654 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5356
SphI G_CATG'C
Cuts at: 1132 2714 1132
Size: 1582 3774
SspI AAT'ATT
Cuts at: 5 52 3280 5
Size: 47 3228 2081
Fragments arranged by size:
3228 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5356
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2100 2280 2520 2595 2795
Size: 552 939 69 27 180 240 75 200
Cuts at: 2795 513
Size: 3074
Fragments arranged by size:
3074 939 552 240 200 180 75 69
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2247 2558 3383 3400 3553 3738 4183
Size: 1255 667 311 825 17 153 185 445
Cuts at: 4183 325
Size: 1498
Fragments arranged by size:
1498 1255 825 667 445 311 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2397 3602
Size: 274 80 33 51 1443 333 87 1205
Cuts at: 3602 5314 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1205 333 274 138 87 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5316 5348 5316
Size: 32 5324
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5356
XbaI T'CTAG_A
Cuts at: 2692 2692
Size: 5356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2287 2011
Size: 276 5080
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5356
XmaI C'CCGG_G
Cuts at: 1870 2579 2681 1870
Size: 709 102 4545
Fragments arranged by size:
4545 709 102
XmnI GAAnn'nnTTC
Cuts at: 3485 3485
Size: 5356
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3164 276
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI
SrfI SwaI