Restriction map for
pFN21AB6967
(Circular) (Six-base) MAPSORT of: pFN21AB6967.seq Check: 4676 from: 1 to: 5350
pFN21AB6967 5350 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2587 3160 278
Size: 53 83 186 936 1051 573 2468
Fragments arranged by size:
2468 1051 936 573 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2693 1937
Size: 756 4594
Acc65I G'GTAC_C
Cuts at: 2671 2671
Size: 5350
AclI AA'CG_TT
Cuts at: 1563 3477 3850 1563
Size: 1914 373 3063
Fragments arranged by size:
3063 1914 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3413 4797 5065 1632
Size: 1781 1384 268 1917
Fragments arranged by size:
1917 1781 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5153
AflIII A'CryG_T
Cuts at: 1203 4249 5226 1203
Size: 3046 977 1327
Fragments arranged by size:
3046 1327 977
AgeI A'CCGG_T
Cuts at: 4181 4181
Size: 5350
AhdI GACnn_n'nnGTC
Cuts at: 4079 4079
Size: 5350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5350
AlwNI CAG_nnn'CTG
Cuts at: 2075 3093 4665 2075
Size: 1018 1572 2760
Fragments arranged by size:
2760 1572 1018
ApaLI G'TGCA_C
Cuts at: 3407 4563 3407
Size: 1156 4194
ApoI r'AATT_y
Cuts at: 1493 2388 2659 2885 3037 5146 1493
Size: 895 271 226 152 2109 1697
Fragments arranged by size:
2109 1697 895 271 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3904 160
Size: 872 2872 1606
Fragments arranged by size:
2872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2675 1870
Size: 73 78 654 4545
Fragments arranged by size:
4545 654 78 73
BamHI G'GATC_C
Cuts at: 2680 3047 2680
Size: 367 4983
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2045 2671 4126 618
Size: 325 217 627 258 626 1455 1842
Fragments arranged by size:
1842 1455 627 626 325 258 217
BanII G_rGCy'C
Cuts at: 1391 2271 2290 2669 1391
Size: 880 19 379 4072
Fragments arranged by size:
4072 880 379 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2099 928
Size: 971 200 4179
Fragments arranged by size:
4179 971 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3541 3575 1903
Size: 34 50 34 1520 34 3678
Fragments arranged by size:
3678 1520 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3242 4452 3242
Size: 1210 4140
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3961 136
Size: 107 122 71 631 605 337 1952 1525
Fragments arranged by size:
1952 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5345 5345
Size: 5350
BlpI GC'TnA_GC
Cuts at: 2767 2767
Size: 5350
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2069 3411 4567 1790
Size: 258 21 1342 1156 2573
Fragments arranged by size:
2573 1342 1156 258 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2145 3081 4039
Size: 416 53 83 399 24 728 936 958
Cuts at: 4039 442
Size: 1753
Fragments arranged by size:
1753 958 936 728 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5350
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2472 2472
Size: 5350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4010 1239
Size: 2771 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3475 4340 4638 3475
Size: 865 298 4187
Fragments arranged by size:
4187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4013 882
Size: 617 2514 2219
Fragments arranged by size:
2514 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3141 493
Size: 711 1937 2702
Fragments arranged by size:
2702 1937 711
BsaBI GATnn'nnATC
Cuts at: 2476 2568 5344 2476
Size: 92 2776 2482
Fragments arranged by size:
2776 2482 92
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2584 3157 3539 4174
Size: 53 83 186 936 1051 573 382 635
Cuts at: 4174 275
Size: 1451
Fragments arranged by size:
1451 1051 936 635 573 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2229 3783 4181 4455 4602 5013 2229
Size: 1554 398 274 147 411 2566
Fragments arranged by size:
2566 1554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5125
Fragments arranged by size:
5125 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2484 1186
Size: 1298 4052
BseYI C'CCAG_C
Cuts at: 2075 4553 5049 2075
Size: 2478 496 2376
Fragments arranged by size:
2478 2376 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2649 1163
Size: 573 913 3864
Fragments arranged by size:
3864 913 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2800 3561 4589 1540
Size: 275 186 799 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2271 2290 3411 3496 4567 2271
Size: 19 1121 85 1071 3054
Fragments arranged by size:
3054 1121 1071 85 19
BsmI GAATG_Cn'
Cuts at: 2861 2954 2861
Size: 93 5257
BsmBI CGTCTCn'nnnn_
Cuts at: 2575 2629 2575
Size: 54 5296
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2069 2271 2290 2669 3411 3496
Size: 399 258 21 202 19 379 742 85
Cuts at: 3496 4567 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 258 202 85
21 19
BspEI T'CCGG_A
Cuts at: 2229 5013 2229
Size: 2784 2566
BspHI T'CATG_A
Cuts at: 3239 4969 3239
Size: 1730 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2003 2383 2705 844
Size: 1159 380 322 3489
Fragments arranged by size:
3489 1159 380 322
BsrBI CCG'CTC
Cuts at: 1394 1823 3237 1394
Size: 429 1414 3507
Fragments arranged by size:
3507 1414 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3845 4019 65
Size: 1183 2597 174 1396
Fragments arranged by size:
2597 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3994 4181 1700
Size: 110 2184 187 2869
Fragments arranged by size:
2869 2184 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5350
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5350
BssSI C'ACGA_G
Cuts at: 2533 3410 4422 2533
Size: 877 1012 3461
Fragments arranged by size:
3461 1012 877
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5350
BstBI TT'CG_AA
Cuts at: 4154 4154
Size: 5350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5350
BstXI CCAn_nnnn'nTGG
Cuts at: 2186 2461 2186
Size: 275 5075
BstYI r'GATC_y
Cuts at: 1972 2680 3047 3432 3449 4901 4987 4999 5345
Size: 708 367 385 17 1452 86 12 346
Cuts at: 5345 1972
Size: 1977
Fragments arranged by size:
1977 1452 708 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4798
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4611
Fragments arranged by size:
4611 683 56
BtsI GCAGTG_nn'
Cuts at: 2866 3660 3680 2866
Size: 794 20 4536
Fragments arranged by size:
4536 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5315
DraI TTT'AAA
Cuts at: 2514 2654 2514
Size: 140 5210
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4357 809
Size: 507 3041 1802
Fragments arranged by size:
3041 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2434 2797 3686
Size: 54 1425 50 275 18 604 363 889
Cuts at: 3686 5315 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 604 363 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2797 1537
Size: 275 985 4090
Fragments arranged by size:
4090 985 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 2590 3280 1353
Size: 1053 184 690 3423
Fragments arranged by size:
3423 1053 690 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3937 4311 4457 387
Size: 1064 138 2348 374 146 1280
Fragments arranged by size:
2348 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2269 2288 2269
Size: 19 5331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3413 4010 4797 5065 1239
Size: 393 1781 597 787 268 1524
Fragments arranged by size:
1781 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2032 2059 2032
Size: 27 5323
EcoRI G'AATT_C
Cuts at: 2659 3037 2659
Size: 378 4972
FspI TGC'GCA
Cuts at: 3856 3856
Size: 5350
HaeII r_GCGC'y
Cuts at: 1678 4497 1678
Size: 2819 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3991 4023 4065 4097 1373
Size: 32 135 32 2419 32 42 32 2626
Fragments arranged by size:
2626 2419 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2018 2694 2940 669
Size: 988 281 80 676 246 3079
Fragments arranged by size:
3079 988 676 281 246 80
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5350
HpaI GTT'AAC
Cuts at: 2940 2940
Size: 5350
KpnI G_GTAC'C
Cuts at: 2675 2675
Size: 5350
MfeI C'AATT_G
Cuts at: 2949 2949
Size: 5350
MluI A'CGCG_T
Cuts at: 5226 5226
Size: 5350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4464 4648 1305
Size: 3159 184 2007
Fragments arranged by size:
3159 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2436 10
Size: 54 1425 343 604 2924
Fragments arranged by size:
2924 1425 604 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2241 3308 3667 3826 518
Size: 648 1075 1067 359 159 2042
Fragments arranged by size:
2042 1075 1067 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2099 2296 2302 2405 2766 3443 4591 4836
Size: 493 197 6 103 361 677 1148 245
Cuts at: 4836 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 493 361 245 197 103
6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4798
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5350
NotI GC'GGCC_GC
Cuts at: 2797 2797
Size: 5350
NspI r_CATG'y
Cuts at: 1132 1229 2708 4253 1132
Size: 97 1479 1545 2229
Fragments arranged by size:
2229 1545 1479 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5350
PciI A'CATG_T
Cuts at: 4249 4249
Size: 5350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2075 1108
Size: 967 4383
PfoI T'CCnGG_A
Cuts at: 2576 2576
Size: 5350
PmeI GTTT'AAAC
Cuts at: 2654 2654
Size: 5350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 4932 4964 3419
Size: 32 1481 32 3805
Fragments arranged by size:
3805 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2059 2032
Size: 27 5323
PshAI GACnn'nnGTC
Cuts at: 1613 2197 1613
Size: 584 4766
PsiI TTA'TAA
Cuts at: 2920 2920
Size: 5350
PspXI vC'TCGA_Gb
Cuts at: 2021 2021
Size: 5350
PstI C_TGCA'G
Cuts at: 830 2295 2301 2329 2702 830
Size: 1465 6 28 373 3478
Fragments arranged by size:
3478 1465 373 28 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5350
PvuII CAG'CTG
Cuts at: 2099 2296 2405 2099
Size: 197 109 5044
Fragments arranged by size:
5044 197 109
SacI G_AGCT'C
Cuts at: 2271 2290 2271
Size: 19 5331
SalI G'TCGA_C
Cuts at: 1936 2692 1936
Size: 756 4594
SanDI GG'GwC_CC
Cuts at: 2032 2032
Size: 5350
SapI GCTCTTCn'nnn_
Cuts at: 2590 2590
Size: 5350
SbfI CC_TGCA'GG
Cuts at: 2702 2702
Size: 5350
ScaI AGT'ACT
Cuts at: 3598 5310 3598
Size: 1712 3638
SfcI C'TryA_G
Cuts at: 826 1045 2291 2297 2325 2698 3833 4514 4705
Size: 219 1246 6 28 373 1135 681 191
Cuts at: 4705 826
Size: 1471
Fragments arranged by size:
1471 1246 1135 681 373 219 191 28
6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5350
SmaI CCC'GGG
Cuts at: 1872 2677 1872
Size: 805 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 3454 4355 4617 820
Size: 197 926 78 1433 901 262 1553
Fragments arranged by size:
1553 1433 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 3141 493
Size: 2648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5350
SphI G_CATG'C
Cuts at: 1132 2708 1132
Size: 1576 3774
SspI AAT'ATT
Cuts at: 5 52 3274 5
Size: 47 3222 2081
Fragments arranged by size:
3222 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5350
StyI C'CwwG_G
Cuts at: 513 1065 2789 513
Size: 552 1724 3074
Fragments arranged by size:
3074 1724 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 2067 3377 3394 3547 3732 4177
Size: 1255 483 4 1310 17 153 185 445
Cuts at: 4177 325
Size: 1498
Fragments arranged by size:
1498 1310 1255 483 445 185 153 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3596 5308 96
Size: 274 80 33 51 1443 1619 1712 138
Fragments arranged by size:
1712 1619 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5310 5342 5310
Size: 32 5318
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5350
XbaI T'CTAG_A
Cuts at: 2686 2686
Size: 5350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 2183
Size: 5350
XhoI C'TCGA_G
Cuts at: 1943 2021 1943
Size: 78 5272
XmaI C'CCGG_G
Cuts at: 1870 2675 1870
Size: 805 4545
XmnI GAAnn'nnTTC
Cuts at: 2389 3479 2389
Size: 1090 4260
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2585 3158 276
Size: 53 83 186 936 1051 573 2468
Fragments arranged by size:
2468 1051 936 573 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI