Restriction map for
pFN21AB6964
(Circular) (Six-base) MAPSORT of: pFN21AB6964.seq Check: 4696 from: 1 to: 5428
pFN21AB6964 5428 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2366 2451 2771 1937
Size: 429 85 320 4594
Fragments arranged by size:
4594 429 320 85
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3491 4875 5143 1632
Size: 1859 1384 268 1917
Fragments arranged by size:
1917 1859 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2716 820
Size: 197 1699 3532
Fragments arranged by size:
3532 1699 197
AflIII A'CryG_T
Cuts at: 1203 4327 5304 1203
Size: 3124 977 1327
Fragments arranged by size:
3124 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 2530 3171 4743 2530
Size: 641 1572 3215
Fragments arranged by size:
3215 1572 641
ApaI G_GGCC'C
Cuts at: 2078 2078
Size: 5428
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2737 2963 3115 5224 1493
Size: 1244 226 152 2109 1697
Fragments arranged by size:
2109 1697 1244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2753 1870
Size: 73 810 4545
Fragments arranged by size:
4545 810 73
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2749 4204 618
Size: 325 217 627 962 1455 1842
Fragments arranged by size:
1842 1455 962 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2078 2747 1391
Size: 687 669 4072
Fragments arranged by size:
4072 687 669
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2614 928
Size: 971 715 3742
Fragments arranged by size:
3742 971 715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3619 3653 1903
Size: 34 1682 34 3678
Fragments arranged by size:
3678 1682 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3320 4530 3320
Size: 1210 4218
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4039 136
Size: 107 122 71 631 605 2367 1525
Fragments arranged by size:
2367 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2506 2845 2506
Size: 339 5089
Bme1580I G_kGCm'C
Cuts at: 1790 2078 3489 4645 1790
Size: 288 1411 1156 2573
Fragments arranged by size:
2573 1411 1156 288
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2664 3159 4117
Size: 416 53 83 399 24 1247 495 958
Cuts at: 4117 442
Size: 1753
Fragments arranged by size:
1753 1247 958 495 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2520 4088 1239
Size: 918 363 1568 2579
Fragments arranged by size:
2579 1568 918 363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3553 4418 4716 3553
Size: 865 298 4265
Fragments arranged by size:
4265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4091 882
Size: 617 2592 2219
Fragments arranged by size:
2592 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3219 493
Size: 711 2015 2702
Fragments arranged by size:
2702 2015 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3235 3617 4252 275
Size: 53 83 186 936 1702 382 635 1451
Fragments arranged by size:
1702 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2667 3861 4259 4533 4680 5091 2667
Size: 1194 398 274 147 411 3004
Fragments arranged by size:
3004 1194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5203
Fragments arranged by size:
5203 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5428
BseYI C'CCAG_C
Cuts at: 4631 5127 4631
Size: 496 4932
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4855
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2878 3639 4667 1540
Size: 275 186 877 761 1028 2301
Fragments arranged by size:
2301 1028 877 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3489 3574 4645 3489
Size: 85 1071 4272
Fragments arranged by size:
4272 1071 85
BsmI GAATG_Cn'
Cuts at: 2317 2939 3032 2317
Size: 622 93 4713
Fragments arranged by size:
4713 622 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2747 3489 3574 4645 1391
Size: 399 288 669 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 399 288 85
BspEI T'CCGG_A
Cuts at: 2667 5091 2667
Size: 2424 3004
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2783 844
Size: 1939 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3315 1394
Size: 429 1492 3507
Fragments arranged by size:
3507 1492 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2364 3923 4097 65
Size: 1183 1116 1559 174 1396
Fragments arranged by size:
1559 1396 1183 1116 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4072 4259 1700
Size: 110 2262 187 2869
Fragments arranged by size:
2869 2262 187 110
BsrGI T'GTAC_A
Cuts at: 96 2649 96
Size: 2553 2875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 3488 4500 3488
Size: 1012 4416
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2156 2354 794
Size: 1362 198 3868
Fragments arranged by size:
3868 1362 198
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstXI CCAn_nnnn'nTGG
Cuts at: 2608 2608
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2670 2758 3125 3510 3527 4979 5065 5077
Size: 698 88 367 385 17 1452 86 12
Cuts at: 5077 5423 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 698 385 367 346 88 86
17 12
BstZ17I GTA'TAC
Cuts at: 2452 2452
Size: 5428
BtgI C'CryG_G
Cuts at: 513 1065 2070 513
Size: 552 1005 3871
Fragments arranged by size:
3871 1005 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3738 3758 2944
Size: 794 20 4614
Fragments arranged by size:
4614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2237 4435 809
Size: 507 921 2198 1802
Fragments arranged by size:
2198 1802 921 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2875 3764 5393
Size: 54 1425 50 275 18 1045 889 1629
Cuts at: 5393 8
Size: 43
Fragments arranged by size:
1629 1425 1045 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2875 1537
Size: 275 1063 4090
Fragments arranged by size:
4090 1063 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3358 1353
Size: 2005 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4015 4389 4535 387
Size: 1064 138 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2520 3491 4088 4875 5143 1239
Size: 393 525 363 971 597 787 268 1524
Fragments arranged by size:
1524 971 787 597 525 393 363 268
EcoO109I rG'GnC_Cy
Cuts at: 2075 2075
Size: 5428
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 2389 4575 1678
Size: 711 2186 2531
Fragments arranged by size:
2531 2186 711
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4069 4101 4143 4175 1373
Size: 32 135 32 2497 32 42 32 2626
Fragments arranged by size:
2626 2497 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2772 3018 669
Size: 988 281 834 246 3079
Fragments arranged by size:
3079 988 834 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5428
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 2130 2151 2349 3027 2130
Size: 21 198 678 4531
Fragments arranged by size:
4531 678 198 21
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4542 4726 1305
Size: 3237 184 2007
Fragments arranged by size:
3237 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3606
Fragments arranged by size:
3606 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3386 3745 3904 518
Size: 648 2220 359 159 2042
Fragments arranged by size:
2220 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2086 2253 2584 2844 3521 4669 4914
Size: 466 14 167 331 260 677 1148 245
Cuts at: 4914 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 466 331 260 245 167
14
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4876
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2563 2579 2786 4331 1132
Size: 97 1334 16 207 1545 2229
Fragments arranged by size:
2229 1545 1334 207 97 16
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5428
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5428
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PspOMI G'GGCC_C
Cuts at: 2074 2074
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2780 830
Size: 1950 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
PvuII CAG'CTG
Cuts at: 2086 2086
Size: 5428
SacII CC_GC'GG
Cuts at: 2073 2073
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SexAI A'CCwGG_T
Cuts at: 2541 2541
Size: 5428
SfcI C'TryA_G
Cuts at: 826 1045 2016 2522 2776 3911 4592 4783 826
Size: 219 971 506 254 1135 681 191 1471
Fragments arranged by size:
1471 1135 971 681 506 254 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2755 1872
Size: 883 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2716 3532 4433 4695 820
Size: 197 926 773 816 901 262 1553
Fragments arranged by size:
1553 926 901 816 773 262 197
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2589 2867 513
Size: 552 1524 278 3074
Fragments arranged by size:
3074 1524 552 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3455 3472 3625 3810 4255 325
Size: 1255 1875 17 153 185 445 1498
Fragments arranged by size:
1875 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 3674 5386
Size: 274 80 33 51 1443 672 1025 1712
Cuts at: 5386 96
Size: 138
Fragments arranged by size:
1712 1443 1025 672 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5388 5420 5388
Size: 32 5396
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5428
XmaI C'CCGG_G
Cuts at: 1870 2753 1870
Size: 883 4545
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI
SwaI XcmI