Restriction map for
pFN21AB6950
(Circular) (Six-base) MAPSORT of: pFN21AB6950.seq Check: 9356 from: 1 to: 5407
pFN21AB6950 5407 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3217 278
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 3470 4854 5122 1632
Size: 1065 773 1384 268 1917
Fragments arranged by size:
1917 1384 1065 773 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 4306 5283 1203
Size: 3103 977 1327
Fragments arranged by size:
3103 1327 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5407
AlwNI CAG_nnn'CTG
Cuts at: 3150 4722 3150
Size: 1572 3835
ApaI G_GGCC'C
Cuts at: 2246 2246
Size: 5407
ApaLI G'TGCA_C
Cuts at: 2264 3464 4620 2264
Size: 1200 1156 3051
Fragments arranged by size:
3051 1200 1156
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2732 1870
Size: 73 789 4545
Fragments arranged by size:
4545 789 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2728 4183 618
Size: 325 217 627 941 1455 1842
Fragments arranged by size:
1842 1455 941 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2104 2246 2275 2726 1391
Size: 713 142 29 451 4072
Fragments arranged by size:
4072 713 451 142 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2522 928
Size: 971 623 3813
Fragments arranged by size:
3813 971 623
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2255 2289 3598 3632 1903
Size: 34 318 34 1309 34 3678
Fragments arranged by size:
3678 1309 318 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5407
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4018 136
Size: 107 122 71 631 605 2346 1525
Fragments arranged by size:
2346 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2545 5402 2545
Size: 2857 2550
BlpI GC'TnA_GC
Cuts at: 2328 2824 2328
Size: 496 4911
Bme1580I G_kGCm'C
Cuts at: 1790 2246 2268 3468 4624 1790
Size: 456 22 1200 1156 2573
Fragments arranged by size:
2573 1200 1156 456 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2685 2685
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 2481 4067 1239
Size: 876 366 1586 2579
Fragments arranged by size:
2579 1586 876 366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2546 3532 4397 4695 2546
Size: 986 865 298 3258
Fragments arranged by size:
3258 986 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4070 882
Size: 617 2571 2219
Fragments arranged by size:
2571 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3198 493
Size: 711 1994 2702
Fragments arranged by size:
2702 1994 711
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3214 3596 4231 275
Size: 53 83 186 936 1681 382 635 1451
Fragments arranged by size:
1681 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3840 4238 4512 4659 5070 3840
Size: 398 274 147 411 4177
Fragments arranged by size:
4177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2003 2033 1178
Size: 30 165 30 600 30 4552
Fragments arranged by size:
4552 600 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2112 1186
Size: 926 4481
BseYI C'CCAG_C
Cuts at: 4610 5106 4610
Size: 496 4911
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4834
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2857 3618 4646 1540
Size: 275 186 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 2275 3468 3553 4624 2268
Size: 7 1193 85 1071 3051
Fragments arranged by size:
3051 1193 1071 85 7
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2246 2268 2275 2726 3468 3553
Size: 399 314 142 22 7 451 742 85
Cuts at: 3553 4624 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 451 399 314 142 85
22 7
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 3296 5026 3296
Size: 1730 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2762 844
Size: 1918 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3294 1394
Size: 429 1471 3507
Fragments arranged by size:
3507 1471 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2496 2650 3902 4076 65
Size: 1183 1248 154 1252 174 1396
Fragments arranged by size:
1396 1252 1248 1183 174 154
BsrFI r'CCGG_y
Cuts at: 1700 1810 4051 4238 1700
Size: 110 2241 187 2869
Fragments arranged by size:
2869 2241 187 110
BsrGI T'GTAC_A
Cuts at: 96 2188 2229 96
Size: 2092 41 3274
Fragments arranged by size:
3274 2092 41
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5407
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5407
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2452 2545 2737 3104 3489 3506 4958 5044
Size: 480 93 192 367 385 17 1452 86
Cuts at: 5044 5056 5402 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 480 385 367 346 192 93
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2109 513
Size: 552 939 105 3811
Fragments arranged by size:
3811 939 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2115 2150 529
Size: 35 1551 35 3786
Fragments arranged by size:
3786 1551 35 35
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2854 3743 5372
Size: 54 1425 50 275 18 1024 889 1629
Cuts at: 5372 8
Size: 43
Fragments arranged by size:
1629 1425 1024 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2854 1537
Size: 275 1042 4090
Fragments arranged by size:
4090 1042 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3337 1353
Size: 1984 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3994 4368 4514 387
Size: 1064 138 435 1970 374 146 1280
Fragments arranged by size:
1970 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2273 2273
Size: 5407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2481 2697 3470 4067 4854 5122
Size: 393 483 366 216 773 597 787 268
Cuts at: 5122 1239
Size: 1524
Fragments arranged by size:
1524 787 773 597 483 393 366 268
216
EcoO109I rG'GnC_Cy
Cuts at: 2243 2243
Size: 5407
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2614 2707 2614
Size: 93 5314
FspI TGC'GCA
Cuts at: 3913 3913
Size: 5407
HaeII r_GCGC'y
Cuts at: 1678 4554 1678
Size: 2876 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373
Size: 32 135 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 2997 669
Size: 988 281 813 246 3079
Fragments arranged by size:
3079 988 813 281 246
HindIII A'AGCT_T
Cuts at: 748 2596 748
Size: 1848 3559
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2399 2614 2696 4521 4705 1305
Size: 1094 215 82 1825 184 2007
Fragments arranged by size:
2007 1825 1094 215 184 82
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3585
Fragments arranged by size:
3585 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3365 3724 3883 518
Size: 648 2199 359 159 2042
Fragments arranged by size:
2199 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2332 2823 3500 4648 4893 1606
Size: 726 491 677 1148 245 2120
Fragments arranged by size:
2120 1148 726 677 491 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3916
Fragments arranged by size:
3916 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2765 4310 1132
Size: 97 1536 1545 2229
Fragments arranged by size:
2229 1545 1536 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5407
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 4989 5021 3476
Size: 32 1481 32 3862
Fragments arranged by size:
3862 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PspOMI G'GGCC_C
Cuts at: 2242 2242
Size: 5407
PstI C_TGCA'G
Cuts at: 830 2759 830
Size: 1929 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
PvuII CAG'CTG
Cuts at: 2332 2332
Size: 5407
SacI G_AGCT'C
Cuts at: 2275 2275
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2088 2322 2484 2755 3890 4571 4762
Size: 219 1043 234 162 271 1135 681 191
Cuts at: 4762 826
Size: 1471
Fragments arranged by size:
1471 1135 1043 681 271 234 219 191
162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2734 1872
Size: 862 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2525 3511 4412 4674 820
Size: 197 926 582 986 901 262 1553
Fragments arranged by size:
1553 986 926 901 582 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2004 2846 513
Size: 552 939 842 3074
Fragments arranged by size:
3074 939 842 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3434 3451 3604 3789 4234 325
Size: 1255 1854 17 153 185 445 1498
Fragments arranged by size:
1854 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2188 2229 3653
Size: 274 80 33 51 1443 211 41 1424
Cuts at: 3653 5365 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1424 274 211 138 80 51
41 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2732 1870
Size: 862 4545
XmnI GAAnn'nnTTC
Cuts at: 2316 2519 3536 2316
Size: 203 1017 4187
Fragments arranged by size:
4187 1017 203
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3215 276
Size: 53 83 186 936 1681 2468
Fragments arranged by size:
2468 1681 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI