Restriction map for
pFN21AB6944
(Circular) (Six-base) MAPSORT of: pFN21AB6944.seq Check: 9642 from: 1 to: 5398
pFN21AB6944 5398 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2329 2329
Size: 5398
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3208 278
Size: 53 83 186 936 1672 2468
Fragments arranged by size:
2468 1672 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2741 1937
Size: 804 4594
Acc65I G'GTAC_C
Cuts at: 2719 2719
Size: 5398
AclI AA'CG_TT
Cuts at: 1563 3525 3898 1563
Size: 1962 373 3063
Fragments arranged by size:
3063 1962 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3461 4845 5113 1632
Size: 1829 1384 268 1917
Fragments arranged by size:
1917 1829 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5201
AflIII A'CryG_T
Cuts at: 1203 2571 4297 5274 1203
Size: 1368 1726 977 1327
Fragments arranged by size:
1726 1368 1327 977
AgeI A'CCGG_T
Cuts at: 4229 4229
Size: 5398
AhdI GACnn_n'nnGTC
Cuts at: 4127 4127
Size: 5398
AleI CACnn'nnGTG
Cuts at: 1166 2042 1166
Size: 876 4522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5398
AlwNI CAG_nnn'CTG
Cuts at: 3141 4713 3141
Size: 1572 3826
ApaI G_GGCC'C
Cuts at: 2512 2512
Size: 5398
ApaLI G'TGCA_C
Cuts at: 2540 3455 4611 2540
Size: 915 1156 3327
Fragments arranged by size:
3327 1156 915
ApoI r'AATT_y
Cuts at: 1493 2707 2933 3085 5194 1493
Size: 1214 226 152 2109 1697
Fragments arranged by size:
2109 1697 1214 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3952 160
Size: 872 2920 1606
Fragments arranged by size:
2920 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5398
AvaI C'yCGr_G
Cuts at: 1870 1943 2397 2505 2723 1870
Size: 73 454 108 218 4545
Fragments arranged by size:
4545 454 218 108 73
BamHI G'GATC_C
Cuts at: 2728 3095 2728
Size: 367 5031
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2309 2552 2719 4174 618
Size: 325 217 627 522 243 167 1455 1842
Fragments arranged by size:
1842 1455 627 522 325 243 217 167
BanII G_rGCy'C
Cuts at: 1391 2187 2512 2626 2691 2717 1391
Size: 796 325 114 65 26 4072
Fragments arranged by size:
4072 796 325 114 65 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2122 928
Size: 971 223 4204
Fragments arranged by size:
4204 971 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3589 3623 1903
Size: 34 1652 34 3678
Fragments arranged by size:
3678 1652 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3290 4500 3290
Size: 1210 4188
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5398
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2601 4009 136
Size: 107 122 71 631 605 929 1408 1525
Fragments arranged by size:
1525 1408 929 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2172 5393 2172
Size: 3221 2177
BlpI GC'TnA_GC
Cuts at: 2815 2815
Size: 5398
Bme1580I G_kGCm'C
Cuts at: 1790 2312 2512 2544 3459 4615 1790
Size: 522 200 32 915 1156 2573
Fragments arranged by size:
2573 1156 915 522 200 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5398
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3129 4087 442
Size: 416 53 83 399 24 1712 958 1753
Fragments arranged by size:
1753 1712 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5398
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2206
Size: 5398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2705 4058 1239
Size: 1037 429 1353 2579
Fragments arranged by size:
2579 1353 1037 429
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3523 4388 4686 3523
Size: 865 298 4235
Fragments arranged by size:
4235 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4061 882
Size: 617 2562 2219
Fragments arranged by size:
2562 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3189 493
Size: 711 1985 2702
Fragments arranged by size:
2702 1985 711
BsaBI GATnn'nnATC
Cuts at: 5392 5392
Size: 5398
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3205 3587 4222 275
Size: 53 83 186 936 1672 382 635 1451
Fragments arranged by size:
1672 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3831 4229 4503 4650 5061 3831
Size: 398 274 147 411 4168
Fragments arranged by size:
4168 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5173
Fragments arranged by size:
5173 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2184 1186
Size: 844 154 4400
Fragments arranged by size:
4400 844 154
BseYI C'CCAG_C
Cuts at: 2023 2410 4601 5097 2023
Size: 387 2191 496 2324
Fragments arranged by size:
2324 2191 496 387
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 2612 1163
Size: 573 330 546 3949
Fragments arranged by size:
3949 573 546 330
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2848 3609 4637 1540
Size: 275 186 847 761 1028 2301
Fragments arranged by size:
2301 1028 847 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2187 2544 2677 3459 3544 4615 2187
Size: 357 133 782 85 1071 2970
Fragments arranged by size:
2970 1071 782 357 133 85
BsmI GAATG_Cn'
Cuts at: 2909 3002 2909
Size: 93 5305
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2312 2512 2544 2626 2677 2691
Size: 399 397 125 200 32 82 51 14
Cuts at: 2691 2717 3459 3544 4615 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 200 125 85
82 51 32 26 14
BspEI T'CCGG_A
Cuts at: 5061 5061
Size: 5398
BspHI T'CATG_A
Cuts at: 2566 3287 5017 2566
Size: 721 1730 2947
Fragments arranged by size:
2947 1730 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2329 2592 2753 844
Size: 1485 263 161 3489
Fragments arranged by size:
3489 1485 263 161
BsrBI CCG'CTC
Cuts at: 1394 1823 3285 1394
Size: 429 1462 3507
Fragments arranged by size:
3507 1462 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3893 4067 65
Size: 1183 2645 174 1396
Fragments arranged by size:
2645 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 4042 4229 1700
Size: 110 231 2001 187 2869
Fragments arranged by size:
2869 2001 231 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5398
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5398
BssSI C'ACGA_G
Cuts at: 3458 4470 3458
Size: 1012 4386
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2635 794
Size: 1841 3557
BstBI TT'CG_AA
Cuts at: 4202 4202
Size: 5398
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5398
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 2293
Size: 5398
BstYI r'GATC_y
Cuts at: 1972 2172 2728 3095 3480 3497 4949 5035 5047
Size: 200 556 367 385 17 1452 86 12
Cuts at: 5047 5393 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 556 385 367 346 200 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2320 513
Size: 552 1255 3591
Fragments arranged by size:
3591 1255 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4659
Fragments arranged by size:
4659 683 56
BtsI GCAGTG_nn'
Cuts at: 2914 3708 3728 2914
Size: 794 20 4584
Fragments arranged by size:
4584 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5363
DraI TTT'AAA
Cuts at: 2702 2702
Size: 5398
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2147 4405 809
Size: 507 831 2258 1802
Fragments arranged by size:
2258 1802 831 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2497 2845 3734
Size: 54 1425 50 275 18 667 348 889
Cuts at: 3734 5363 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 667 348 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2845 1537
Size: 275 1033 4090
Fragments arranged by size:
4090 1033 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2192 3328 1353
Size: 839 1136 3423
Fragments arranged by size:
3423 1136 839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3985 4359 4505 387
Size: 1064 138 2396 374 146 1280
Fragments arranged by size:
2396 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2185 2185
Size: 5398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2276 2705 3461 4058 4845 5113 1239
Size: 393 644 429 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 644 597 429 393 268
EcoRI G'AATT_C
Cuts at: 2707 3085 2707
Size: 378 5020
FspI TGC'GCA
Cuts at: 3904 3904
Size: 5398
HaeII r_GCGC'y
Cuts at: 1678 2071 4545 1678
Size: 393 2474 2531
Fragments arranged by size:
2531 2474 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4039 4071 4113 4145 1373
Size: 32 135 32 2467 32 42 32 2626
Fragments arranged by size:
2626 2467 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2742 2988 669
Size: 988 281 804 246 3079
Fragments arranged by size:
3079 988 804 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5398
HpaI GTT'AAC
Cuts at: 2988 2988
Size: 5398
KpnI G_GTAC'C
Cuts at: 2723 2723
Size: 5398
MfeI C'AATT_G
Cuts at: 2997 2997
Size: 5398
MluI A'CGCG_T
Cuts at: 5274 5274
Size: 5398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4512 4696 1305
Size: 3207 184 2007
Fragments arranged by size:
3207 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2499 10
Size: 54 1425 343 667 2909
Fragments arranged by size:
2909 1425 667 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2547 3356 3715 3874 518
Size: 648 876 505 809 359 159 2042
Fragments arranged by size:
2042 876 809 648 505 359 159
MspA1I CmG'CkG
Cuts at: 1606 2814 3491 4639 4884 1606
Size: 1208 677 1148 245 2120
Fragments arranged by size:
2120 1208 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4846
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5398
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5398
NotI GC'GGCC_GC
Cuts at: 2845 2845
Size: 5398
NspI r_CATG'y
Cuts at: 1132 1229 2204 2243 2575 2756 4301 1132
Size: 97 975 39 332 181 1545 2229
Fragments arranged by size:
2229 1545 975 332 181 97 39
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5398
PciI A'CATG_T
Cuts at: 2571 4297 2571
Size: 1726 3672
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5398
PmeI GTTT'AAAC
Cuts at: 2702 2702
Size: 5398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3467 3499 4980 5012 3467
Size: 32 1481 32 3853
Fragments arranged by size:
3853 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5398
PsiI TTA'TAA
Cuts at: 2968 2968
Size: 5398
PspOMI G'GGCC_C
Cuts at: 2508 2508
Size: 5398
PstI C_TGCA'G
Cuts at: 830 2750 830
Size: 1920 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5398
SacI G_AGCT'C
Cuts at: 2187 2187
Size: 5398
SalI G'TCGA_C
Cuts at: 1936 2740 1936
Size: 804 4594
SapI GCTCTTCn'nnn_
Cuts at: 2192 2192
Size: 5398
SbfI CC_TGCA'GG
Cuts at: 2750 2750
Size: 5398
ScaI AGT'ACT
Cuts at: 3646 5358 3646
Size: 1712 3686
SfcI C'TryA_G
Cuts at: 826 1045 2746 3881 4562 4753 826
Size: 219 1701 1135 681 191 1471
Fragments arranged by size:
1701 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5398
SgrAI Cr'CCGG_yG
Cuts at: 1700 2041 1700
Size: 341 5057
SmaI CCC'GGG
Cuts at: 1872 2399 2725 1872
Size: 527 326 4545
Fragments arranged by size:
4545 527 326
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3502 4403 4665 820
Size: 197 926 1559 901 262 1553
Fragments arranged by size:
1559 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3189 493
Size: 2696 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5398
SphI G_CATG'C
Cuts at: 1132 2204 2756 1132
Size: 1072 552 3774
Fragments arranged by size:
3774 1072 552
SspI AAT'ATT
Cuts at: 5 52 3322 5
Size: 47 3270 2081
Fragments arranged by size:
3270 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5398
StyI C'CwwG_G
Cuts at: 513 1065 2145 2668 2837 513
Size: 552 1080 523 169 3074
Fragments arranged by size:
3074 1080 552 523 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2293 3425 3442 3595 3780 4225 325
Size: 1255 713 1132 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1132 713 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2242 3644 5356
Size: 274 80 33 51 1443 265 1402 1712
Cuts at: 5356 96
Size: 138
Fragments arranged by size:
1712 1443 1402 274 265 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5358 5390 5358
Size: 32 5366
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5398
XbaI T'CTAG_A
Cuts at: 2734 2734
Size: 5398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2293 2368 2293
Size: 75 5323
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5398
XmaI C'CCGG_G
Cuts at: 1870 2397 2723 1870
Size: 527 326 4545
Fragments arranged by size:
4545 527 326
XmnI GAAnn'nnTTC
Cuts at: 3527 3527
Size: 5398
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3206 276
Size: 53 83 186 936 1672 2468
Fragments arranged by size:
2468 1672 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI