Restriction map for
pFN21AB6940
(Circular) (Six-base) MAPSORT of: pFN21AB6940.seq Check: 9499 from: 1 to: 5392
pFN21AB6940 5392 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2339 2339
Size: 5392
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3202 278
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2735 1937
Size: 798 4594
Acc65I G'GTAC_C
Cuts at: 2713 2713
Size: 5392
AclI AA'CG_TT
Cuts at: 1563 3519 3892 1563
Size: 1956 373 3063
Fragments arranged by size:
3063 1956 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3455 4839 5107 1632
Size: 1823 1384 268 1917
Fragments arranged by size:
1917 1823 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5195
AflIII A'CryG_T
Cuts at: 1203 2242 2574 4291 5268 1203
Size: 1039 332 1717 977 1327
Fragments arranged by size:
1717 1327 1039 977 332
AgeI A'CCGG_T
Cuts at: 4223 4223
Size: 5392
AhdI GACnn_n'nnGTC
Cuts at: 4121 4121
Size: 5392
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2635 2645 1184
Size: 1451 10 3931
Fragments arranged by size:
3931 1451 10
AlwNI CAG_nnn'CTG
Cuts at: 2521 3135 4707 2521
Size: 614 1572 3206
Fragments arranged by size:
3206 1572 614
ApaLI G'TGCA_C
Cuts at: 2543 3449 4605 2543
Size: 906 1156 3330
Fragments arranged by size:
3330 1156 906
ApoI r'AATT_y
Cuts at: 1493 2212 2701 2927 3079 5188 1493
Size: 719 489 226 152 2109 1697
Fragments arranged by size:
2109 1697 719 489 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3946 160
Size: 872 2914 1606
Fragments arranged by size:
2914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
AvaI C'yCGr_G
Cuts at: 1870 1943 2427 2717 1870
Size: 73 484 290 4545
Fragments arranged by size:
4545 484 290 73
BamHI G'GATC_C
Cuts at: 2722 3089 2722
Size: 367 5025
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2555 2713 4168 618
Size: 325 217 627 768 158 1455 1842
Fragments arranged by size:
1842 1455 768 627 325 217 158
BanII G_rGCy'C
Cuts at: 1391 2711 1391
Size: 1320 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3583 3617 1903
Size: 34 1646 34 3678
Fragments arranged by size:
3678 1646 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2518 3284 4494 2518
Size: 766 1210 3416
Fragments arranged by size:
3416 1210 766
BclI T'GATC_A
Cuts at: 1548 2640 1548
Size: 1092 4300
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4003 136
Size: 107 122 71 631 605 2331 1525
Fragments arranged by size:
2331 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2616 5387 2616
Size: 2771 2621
BlpI GC'TnA_GC
Cuts at: 2809 2809
Size: 5392
Bme1580I G_kGCm'C
Cuts at: 1790 2547 3453 4609 1790
Size: 757 906 1156 2573
Fragments arranged by size:
2573 1156 906 757
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5392
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3123 4081 442
Size: 416 53 83 399 24 1706 958 1753
Fragments arranged by size:
1753 1706 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 4052 1239
Size: 1055 1758 2579
Fragments arranged by size:
2579 1758 1055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 3517 4382 4680 2468
Size: 1049 865 298 3180
Fragments arranged by size:
3180 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4055 882
Size: 617 2556 2219
Fragments arranged by size:
2556 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3183 493
Size: 711 1979 2702
Fragments arranged by size:
2702 1979 711
BsaBI GATnn'nnATC
Cuts at: 5386 5386
Size: 5392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3199 3581 4216 275
Size: 53 83 186 936 1666 382 635 1451
Fragments arranged by size:
1666 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3825 4223 4497 4644 5055 3825
Size: 398 274 147 411 4162
Fragments arranged by size:
4162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5167
Fragments arranged by size:
5167 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2471 2684 1186
Size: 1285 213 3894
Fragments arranged by size:
3894 1285 213
BseYI C'CCAG_C
Cuts at: 2026 4595 5091 2026
Size: 2569 496 2327
Fragments arranged by size:
2569 2327 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2534 1163
Size: 573 438 360 4021
Fragments arranged by size:
4021 573 438 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3603 4631 1540
Size: 275 186 841 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2547 3453 3538 4609 2547
Size: 906 85 1071 3330
Fragments arranged by size:
3330 1071 906 85
BsmI GAATG_Cn'
Cuts at: 2903 2996 2903
Size: 93 5299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2547 2711 3453 3538 4609 1391
Size: 399 757 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 757 742 399 164 85
BspEI T'CCGG_A
Cuts at: 5055 5055
Size: 5392
BspHI T'CATG_A
Cuts at: 3281 5011 3281
Size: 1730 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2339 2747 844
Size: 1495 408 3489
Fragments arranged by size:
3489 1495 408
BsrBI CCG'CTC
Cuts at: 1394 1823 3279 1394
Size: 429 1456 3507
Fragments arranged by size:
3507 1456 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2685 3887 4061 65
Size: 1183 1437 1202 174 1396
Fragments arranged by size:
1437 1396 1202 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4036 4223 1700
Size: 110 2226 187 2869
Fragments arranged by size:
2869 2226 187 110
BsrGI T'GTAC_A
Cuts at: 96 2368 96
Size: 2272 3120
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5392
BssSI C'ACGA_G
Cuts at: 3452 4464 3452
Size: 1012 4380
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2161 794
Size: 1367 4025
BstBI TT'CG_AA
Cuts at: 4196 4196
Size: 5392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5392
BstYI r'GATC_y
Cuts at: 1972 2616 2722 3089 3474 3491 4943 5029 5041
Size: 644 106 367 385 17 1452 86 12
Cuts at: 5041 5387 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 644 385 367 346 106 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2289 2667 2289
Size: 378 5014
BtgI C'CryG_G
Cuts at: 513 1065 2148 513
Size: 552 1083 3757
Fragments arranged by size:
3757 1083 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4653
Fragments arranged by size:
4653 683 56
BtsI GCAGTG_nn'
Cuts at: 2908 3702 3722 2908
Size: 794 20 4578
Fragments arranged by size:
4578 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5357
DraI TTT'AAA
Cuts at: 2696 2696
Size: 5392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4399 809
Size: 507 3083 1802
Fragments arranged by size:
3083 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2839 3728 5357
Size: 54 1425 50 275 18 1009 889 1629
Cuts at: 5357 8
Size: 43
Fragments arranged by size:
1629 1425 1009 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 1537
Size: 275 1027 4090
Fragments arranged by size:
4090 1027 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3322 1353
Size: 1969 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3979 4353 4499 387
Size: 1064 138 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 3455 4052 4839 5107 1239
Size: 393 662 1161 597 787 268 1524
Fragments arranged by size:
1524 1161 787 662 597 393 268
EcoRI G'AATT_C
Cuts at: 2701 3079 2701
Size: 378 5014
FspI TGC'GCA
Cuts at: 3898 3898
Size: 5392
HaeII r_GCGC'y
Cuts at: 1678 4539 1678
Size: 2861 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4033 4065 4107 4139 1373
Size: 32 135 32 2461 32 42 32 2626
Fragments arranged by size:
2626 2461 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 2982 669
Size: 988 281 798 246 3079
Fragments arranged by size:
3079 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 2501 748
Size: 1753 3639
HpaI GTT'AAC
Cuts at: 2982 2982
Size: 5392
KpnI G_GTAC'C
Cuts at: 2717 2717
Size: 5392
MfeI C'AATT_G
Cuts at: 2991 2991
Size: 5392
MluI A'CGCG_T
Cuts at: 5268 5268
Size: 5392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 4506 4690 1305
Size: 1329 1872 184 2007
Fragments arranged by size:
2007 1872 1329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3570
Fragments arranged by size:
3570 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3350 3709 3868 518
Size: 648 2184 359 159 2042
Fragments arranged by size:
2184 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2808 3485 4633 4878 1606
Size: 1202 677 1148 245 2120
Fragments arranged by size:
2120 1202 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2148 513
Size: 552 1083 3757
Fragments arranged by size:
3757 1083 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5392
NotI GC'GGCC_GC
Cuts at: 2839 2839
Size: 5392
NspI r_CATG'y
Cuts at: 1132 1229 2045 2246 2578 2750 4295 1132
Size: 97 816 201 332 172 1545 2229
Fragments arranged by size:
2229 1545 816 332 201 172 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5392
PciI A'CATG_T
Cuts at: 2242 2574 4291 2242
Size: 332 1717 3343
Fragments arranged by size:
3343 1717 332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5392
PfoI T'CCnGG_A
Cuts at: 2520 2520
Size: 5392
PmeI GTTT'AAAC
Cuts at: 2696 2696
Size: 5392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 4974 5006 3461
Size: 32 1481 32 3847
Fragments arranged by size:
3847 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5392
PsiI TTA'TAA
Cuts at: 2962 2962
Size: 5392
PspXI vC'TCGA_Gb
Cuts at: 2427 2427
Size: 5392
PstI C_TGCA'G
Cuts at: 830 2744 830
Size: 1914 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5392
SalI G'TCGA_C
Cuts at: 1936 2734 1936
Size: 798 4594
SbfI CC_TGCA'GG
Cuts at: 2744 2744
Size: 5392
ScaI AGT'ACT
Cuts at: 3640 5352 3640
Size: 1712 3680
SfcI C'TryA_G
Cuts at: 826 1045 2740 3875 4556 4747 826
Size: 219 1695 1135 681 191 1471
Fragments arranged by size:
1695 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5392
SmaI CCC'GGG
Cuts at: 1872 2719 1872
Size: 847 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2427 2483 3496 4397 4659 820
Size: 197 926 484 56 1013 901 262 1553
Fragments arranged by size:
1553 1013 926 901 484 262 197 56
SnaBI TAC'GTA
Cuts at: 493 3183 493
Size: 2690 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5392
SphI G_CATG'C
Cuts at: 1132 2045 2750 1132
Size: 913 705 3774
Fragments arranged by size:
3774 913 705
SspI AAT'ATT
Cuts at: 5 52 2281 3316 5
Size: 47 2229 1035 2081
Fragments arranged by size:
2229 2081 1035 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5392
StyI C'CwwG_G
Cuts at: 513 1065 2011 2148 2466 2831 513
Size: 552 946 137 318 365 3074
Fragments arranged by size:
3074 946 552 365 318 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2085 3419 3436 3589 3774 4219 325
Size: 1255 505 1334 17 153 185 445 1498
Fragments arranged by size:
1498 1334 1255 505 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2128 2368 3638
Size: 274 80 33 51 1443 151 240 1270
Cuts at: 3638 5350 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1270 274 240 151 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5352 5384 5352
Size: 32 5360
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5392
XbaI T'CTAG_A
Cuts at: 2728 2728
Size: 5392
XhoI C'TCGA_G
Cuts at: 1943 2427 1943
Size: 484 4908
XmaI C'CCGG_G
Cuts at: 1870 2717 1870
Size: 847 4545
XmnI GAAnn'nnTTC
Cuts at: 2055 3521 2055
Size: 1466 3926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3200 276
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI