Restriction map for
pFN21AB6937
(Circular) (Six-base) MAPSORT of: pFN21AB6937.seq Check: 4062 from: 1 to: 5335
pFN21AB6937 5335 bp
With 182 enzymes: SgfI *
December 25, 2009 11:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2396 2396
Size: 5335
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2307 2678 1937
Size: 370 371 4594
Fragments arranged by size:
4594 371 370
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 3398 4782 5050 1632
Size: 947 819 1384 268 1917
Fragments arranged by size:
1917 1384 947 819 268
AfeI AGC'GCT
Cuts at: 2438 2438
Size: 5335
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 2412 4234 5211 1203
Size: 1209 1822 977 1327
Fragments arranged by size:
1822 1327 1209 977
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 4064 4064
Size: 5335
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5335
AlwNI CAG_nnn'CTG
Cuts at: 3078 4650 3078
Size: 1572 3763
ApaI G_GGCC'C
Cuts at: 2075 2075
Size: 5335
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2542 2644 2870 3022 5131 1493
Size: 1049 102 226 152 2109 1697
Fragments arranged by size:
2109 1697 1049 226 152 102
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2038 2088 2104 2660 1870
Size: 73 72 23 50 16 556 4545
Fragments arranged by size:
4545 556 73 72 50 23 16
BamHI G'GATC_C
Cuts at: 2665 3032 2665
Size: 367 4968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 2172 2656 4111 618
Size: 325 217 627 290 95 484 1455 1842
Fragments arranged by size:
1842 1455 627 484 325 290 217 95
BanII G_rGCy'C
Cuts at: 1391 2075 2103 2348 2654 1391
Size: 684 28 245 306 4072
Fragments arranged by size:
4072 684 306 245 28
BbeI G_GCGC'C
Cuts at: 2081 2176 2081
Size: 95 5240
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2239 928
Size: 971 340 4024
Fragments arranged by size:
4024 971 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3526 3560 1903
Size: 34 50 34 1505 34 3678
Fragments arranged by size:
3678 1505 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5335
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2057 2107 3946
Size: 107 122 71 631 605 385 50 1839
Cuts at: 3946 136
Size: 1525
Fragments arranged by size:
1839 1525 631 605 385 122 107 71
50
BglII A'GATC_T
Cuts at: 5330 5330
Size: 5335
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 2075 3396 4552 1790
Size: 285 1321 1156 2573
Fragments arranged by size:
2573 1321 1156 285
BmgBI CAC'GTC
Cuts at: 1318 2030 1318
Size: 712 4623
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3066 4024 442
Size: 416 53 83 399 24 1649 958 1753
Fragments arranged by size:
1753 1649 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 2528 3995 1239
Size: 1229 60 1467 2579
Fragments arranged by size:
2579 1467 1229 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3460 4325 4623 3460
Size: 865 298 4172
Fragments arranged by size:
4172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3998 882
Size: 617 2499 2219
Fragments arranged by size:
2499 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2338 3126 493
Size: 711 1134 788 2702
Fragments arranged by size:
2702 1134 788 711
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 2173 3142 3524
Size: 53 83 186 936 545 95 969 382
Cuts at: 3524 4159 275
Size: 635 1451
Fragments arranged by size:
1451 969 936 635 545 382 186 95
83 53
BsaWI w'CCGG_w
Cuts at: 3768 4166 4440 4587 4998 3768
Size: 398 274 147 411 4105
Fragments arranged by size:
4105 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5110
Fragments arranged by size:
5110 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5335
BseYI C'CCAG_C
Cuts at: 2520 4538 5034 2520
Size: 2018 496 2821
Fragments arranged by size:
2821 2018 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4762
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2785 3546 4574 1540
Size: 275 186 784 761 1028 2301
Fragments arranged by size:
2301 1028 784 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3396 3481 4552 3396
Size: 85 1071 4179
Fragments arranged by size:
4179 1071 85
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2103 2348 2654 3396 3481 4552
Size: 399 285 28 245 306 742 85 1071
Cuts at: 4552 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 306 285 245 85
28
BspEI T'CCGG_A
Cuts at: 4998 4998
Size: 5335
BspHI T'CATG_A
Cuts at: 3224 4954 3224
Size: 1730 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2396 2690 844
Size: 1552 294 3489
Fragments arranged by size:
3489 1552 294
BsrBI CCG'CTC
Cuts at: 1394 1823 3222 1394
Size: 429 1399 3507
Fragments arranged by size:
3507 1399 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3830 4004 65
Size: 1183 2582 174 1396
Fragments arranged by size:
2582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2175 3979 4166 1700
Size: 110 365 1804 187 2869
Fragments arranged by size:
2869 1804 365 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5335
BssSI C'ACGA_G
Cuts at: 3395 4407 3395
Size: 1012 4323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5335
BstBI TT'CG_AA
Cuts at: 4139 4139
Size: 5335
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5335
BstYI r'GATC_y
Cuts at: 1972 2665 3032 3417 3434 4886 4972 4984 5330
Size: 693 367 385 17 1452 86 12 346
Cuts at: 5330 1972
Size: 1977
Fragments arranged by size:
1977 1452 693 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2052 513
Size: 552 987 3796
Fragments arranged by size:
3796 987 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4596
Fragments arranged by size:
4596 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3645 3665 2851
Size: 794 20 4521
Fragments arranged by size:
4521 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5335
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5300
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4342 809
Size: 507 3026 1802
Fragments arranged by size:
3026 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 2184 2782
Size: 54 1425 50 275 18 194 160 598
Cuts at: 2782 3671 5300 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 598 275 194 160 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2782 1537
Size: 275 970 4090
Fragments arranged by size:
4090 970 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 3265 1353
Size: 980 932 3423
Fragments arranged by size:
3423 980 932
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3922 4296 4442 387
Size: 1064 138 2333 374 146 1280
Fragments arranged by size:
2333 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 2528 2579 3398 3995 4782 5050
Size: 393 836 60 51 819 597 787 268
Cuts at: 5050 1239
Size: 1524
Fragments arranged by size:
1524 836 819 787 597 393 268 60
51
EcoO109I rG'GnC_Cy
Cuts at: 2071 2071
Size: 5335
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 3841 3841
Size: 5335
HaeII r_GCGC'y
Cuts at: 1678 2081 2176 2440 4482 1678
Size: 403 95 264 2042 2531
Fragments arranged by size:
2531 2042 403 264 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3976 4008 4050 4082 1373
Size: 32 135 32 2404 32 42 32 2626
Fragments arranged by size:
2626 2404 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 2925 669
Size: 988 281 741 246 3079
Fragments arranged by size:
3079 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KasI G'GCGC_C
Cuts at: 2077 2172 2077
Size: 95 5240
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4449 4633 1305
Size: 3144 184 2007
Fragments arranged by size:
3144 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2026 2186 10
Size: 54 1425 343 194 160 3159
Fragments arranged by size:
3159 1425 343 194 160 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3293 3652 3811 518
Size: 648 2127 359 159 2042
Fragments arranged by size:
2127 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2054 2751 3428 4576 4821 1606
Size: 405 43 697 677 1148 245 2120
Fragments arranged by size:
2120 1148 697 677 405 245 43
NaeI GCC'GGC
Cuts at: 2177 2177
Size: 5335
NarI GG'CG_CC
Cuts at: 2078 2173 2078
Size: 95 5240
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4783
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NgoMIV G'CCGG_C
Cuts at: 2175 2175
Size: 5335
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2782 2782
Size: 5335
NspI r_CATG'y
Cuts at: 1132 1229 2416 2693 4238 1132
Size: 97 1187 277 1545 2229
Fragments arranged by size:
2229 1545 1187 277 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5335
PciI A'CATG_T
Cuts at: 2412 4234 2412
Size: 1822 3513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5335
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2115 2147 3404 3436 4917 4949 2115
Size: 32 1257 32 1481 32 2501
Fragments arranged by size:
2501 1481 1257 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2303 1613
Size: 690 4645
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PspOMI G'GGCC_C
Cuts at: 2071 2071
Size: 5335
PspXI vC'TCGA_Gb
Cuts at: 2088 2088
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2687 830
Size: 1857 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
SacII CC_GC'GG
Cuts at: 2055 2055
Size: 5335
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SbfI CC_TGCA'GG
Cuts at: 2687 2687
Size: 5335
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SfcI C'TryA_G
Cuts at: 826 1045 2683 3818 4499 4690 826
Size: 219 1638 1135 681 191 1471
Fragments arranged by size:
1638 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2057 2057
Size: 5335
SfoI GGC'GCC
Cuts at: 2079 2174 2079
Size: 95 5240
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2040 2662 1872
Size: 168 622 4545
Fragments arranged by size:
4545 622 168
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2088 3439 4340 4602 820
Size: 197 926 145 1351 901 262 1553
Fragments arranged by size:
1553 1351 926 901 262 197 145
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2693 1132
Size: 1561 3774
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 2021 1517
Size: 504 4831
StyI C'CwwG_G
Cuts at: 513 1065 2604 2774 513
Size: 552 1539 170 3074
Fragments arranged by size:
3074 1539 552 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3362 3379 3532 3717 4162 325
Size: 1255 1782 17 153 185 445 1498
Fragments arranged by size:
1782 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3581 5293 96
Size: 274 80 33 51 1443 1604 1712 138
Fragments arranged by size:
1712 1604 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2257 2289 5295 5327 2257
Size: 32 3006 32 2265
Fragments arranged by size:
3006 2265 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2671 2671
Size: 5335
XhoI C'TCGA_G
Cuts at: 1943 2088 1943
Size: 145 5190
XmaI C'CCGG_G
Cuts at: 1870 2038 2660 1870
Size: 168 622 4545
Fragments arranged by size:
4545 622 168
XmnI GAAnn'nnTTC
Cuts at: 3464 3464
Size: 5335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PsrI PvuII RsrII SacI SanDI SapI
SexAI SrfI SwaI XcmI