Restriction map for
pFN21AB6936
(Circular) (Six-base) MAPSORT of: pFN21AB6936.seq Check: 6789 from: 1 to: 5392
pFN21AB6936 5392 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3202 278
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2735 1937
Size: 798 4594
Acc65I G'GTAC_C
Cuts at: 2121 2713 2121
Size: 592 4800
AclI AA'CG_TT
Cuts at: 1563 3519 3892 1563
Size: 1956 373 3063
Fragments arranged by size:
3063 1956 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2513 3455 4839 5107 1632
Size: 881 942 1384 268 1917
Fragments arranged by size:
1917 1384 942 881 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5195
AflIII A'CryG_T
Cuts at: 1203 4291 5268 1203
Size: 3088 977 1327
Fragments arranged by size:
3088 1327 977
AgeI A'CCGG_T
Cuts at: 4223 4223
Size: 5392
AhdI GACnn_n'nnGTC
Cuts at: 4121 4121
Size: 5392
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2375 1184
Size: 1191 4201
AlwNI CAG_nnn'CTG
Cuts at: 3135 4707 3135
Size: 1572 3820
ApaI G_GGCC'C
Cuts at: 2076 2550 2076
Size: 474 4918
ApaLI G'TGCA_C
Cuts at: 3449 4605 3449
Size: 1156 4236
ApoI r'AATT_y
Cuts at: 1493 2701 2927 3079 5188 1493
Size: 1208 226 152 2109 1697
Fragments arranged by size:
2109 1697 1208 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3946 160
Size: 872 2914 1606
Fragments arranged by size:
2914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2220 2459 2589 2717 1870
Size: 73 154 123 239 130 128 4545
Fragments arranged by size:
4545 239 154 130 128 123 73
BamHI G'GATC_C
Cuts at: 2502 2722 3089 2502
Size: 220 367 4805
Fragments arranged by size:
4805 367 220
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2121 2713 4168 618
Size: 325 217 627 334 592 1455 1842
Fragments arranged by size:
1842 1455 627 592 334 325 217
BanII G_rGCy'C
Cuts at: 1391 2076 2345 2550 2711 1391
Size: 685 269 205 161 4072
Fragments arranged by size:
4072 685 269 205 161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2338 2372 3583 3617 1903
Size: 34 401 34 1211 34 3678
Fragments arranged by size:
3678 1211 401 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3284 4494 3284
Size: 1210 4182
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5392
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4003 136
Size: 107 122 71 631 605 2331 1525
Fragments arranged by size:
2331 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2481 5387 2481
Size: 2906 2486
BlpI GC'TnA_GC
Cuts at: 2427 2809 2427
Size: 382 5010
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2550 3453 4609 1790
Size: 286 474 903 1156 2573
Fragments arranged by size:
2573 1156 903 474 286
BmgBI CAC'GTC
Cuts at: 1318 2562 1318
Size: 1244 4148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3123 4081 442
Size: 416 53 83 399 24 1706 958 1753
Fragments arranged by size:
1753 1706 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5392
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 5392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2329 2382 2674 4052 1239
Size: 1090 53 292 1378 2579
Fragments arranged by size:
2579 1378 1090 292 53
Bpu10I CC'TnA_GC
Cuts at: 2414 2619 2414
Size: 205 5187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3517 4382 4680 3517
Size: 865 298 4229
Fragments arranged by size:
4229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4055 882
Size: 617 2556 2219
Fragments arranged by size:
2556 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2199 2311 3183 493
Size: 711 995 112 872 2702
Fragments arranged by size:
2702 995 872 711 112
BsaBI GATnn'nnATC
Cuts at: 5386 5386
Size: 5392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2252 3199 3581 4216
Size: 53 83 186 936 719 947 382 635
Cuts at: 4216 275
Size: 1451
Fragments arranged by size:
1451 947 936 719 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3825 4223 4497 4644 5055 3825
Size: 398 274 147 411 4162
Fragments arranged by size:
4162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5167
Fragments arranged by size:
5167 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5392
BseYI C'CCAG_C
Cuts at: 2077 4595 5091 2077
Size: 2518 496 2378
Fragments arranged by size:
2518 2378 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2254 2465 1163
Size: 573 518 211 4090
Fragments arranged by size:
4090 573 518 211
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 2842 3603 4631 1540
Size: 275 186 129 712 761 1028 2301
Fragments arranged by size:
2301 1028 761 712 275 186 129
BsiHKAI G_wGCw'C
Cuts at: 2316 2379 3453 3538 4609 2316
Size: 63 1074 85 1071 3099
Fragments arranged by size:
3099 1074 1071 85 63
BsmI GAATG_Cn'
Cuts at: 2903 2996 2903
Size: 93 5299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2316 2345 2379 2550 2711 3453
Size: 399 286 240 29 34 171 161 742
Cuts at: 3453 3538 4609 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 286 240 171 161
85 34 29
BspEI T'CCGG_A
Cuts at: 5055 5055
Size: 5392
BspHI T'CATG_A
Cuts at: 3281 5011 3281
Size: 1730 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 844
Size: 1903 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3279 1394
Size: 429 1456 3507
Fragments arranged by size:
3507 1456 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2111 2190 3887 4061 65
Size: 1183 863 79 1697 174 1396
Fragments arranged by size:
1697 1396 1183 863 174 79
BsrFI r'CCGG_y
Cuts at: 1700 1810 4036 4223 1700
Size: 110 2226 187 2869
Fragments arranged by size:
2869 2226 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5392
BssHII G'CGCG_C
Cuts at: 1925 2528 1925
Size: 603 4789
BssSI C'ACGA_G
Cuts at: 3452 4464 3452
Size: 1012 4380
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5392
BstBI TT'CG_AA
Cuts at: 4196 4196
Size: 5392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5392
BstYI r'GATC_y
Cuts at: 1972 2481 2502 2722 3089 3474 3491 4943 5029
Size: 509 21 220 367 385 17 1452 86
Cuts at: 5029 5041 5387 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 509 385 367 346 220 86
21 17 12
Bsu36I CC'TnA_GG
Cuts at: 2573 2573
Size: 5392
BtgI C'CryG_G
Cuts at: 513 1065 2133 2298 513
Size: 552 1068 165 3607
Fragments arranged by size:
3607 1068 552 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4653
Fragments arranged by size:
4653 683 56
BtsI GCAGTG_nn'
Cuts at: 2065 2090 2453 2667 2908 3702 3722 2065
Size: 25 363 214 241 794 20 3735
Fragments arranged by size:
3735 794 363 241 214 25 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5357
DraI TTT'AAA
Cuts at: 2696 2696
Size: 5392
DraIII CAC_nnn'GTG
Cuts at: 2542 2542
Size: 5392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2257 4399 809
Size: 507 941 2142 1802
Fragments arranged by size:
2142 1802 941 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2839 3728 5357
Size: 54 1425 50 275 18 1009 889 1629
Cuts at: 5357 8
Size: 43
Fragments arranged by size:
1629 1425 1009 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 1537
Size: 275 1027 4090
Fragments arranged by size:
4090 1027 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3322 1353
Size: 1969 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3979 4353 4499 387
Size: 1064 138 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2329 2382 2513 2674 3455 4052 4839
Size: 393 697 53 131 161 781 597 787
Cuts at: 4839 5107 1239
Size: 268 1524
Fragments arranged by size:
1524 787 781 697 597 393 268 161
131 53
EcoNI CCTnn'n_nnAGG
Cuts at: 2400 2687 2400
Size: 287 5105
EcoO109I rG'GnC_Cy
Cuts at: 2072 2073 2463 2546 2623 2072
Size: 1 390 83 77 4841
Fragments arranged by size:
4841 390 83 77 1
EcoRI G'AATT_C
Cuts at: 2701 3079 2701
Size: 378 5014
FspI TGC'GCA
Cuts at: 3898 3898
Size: 5392
HaeII r_GCGC'y
Cuts at: 1678 2434 4539 1678
Size: 756 2105 2531
Fragments arranged by size:
2531 2105 756
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4033 4065 4107 4139 1373
Size: 32 135 32 2461 32 42 32 2626
Fragments arranged by size:
2626 2461 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 2982 669
Size: 988 281 798 246 3079
Fragments arranged by size:
3079 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5392
HpaI GTT'AAC
Cuts at: 2982 2982
Size: 5392
KpnI G_GTAC'C
Cuts at: 2125 2717 2125
Size: 592 4800
MfeI C'AATT_G
Cuts at: 2991 2991
Size: 5392
MluI A'CGCG_T
Cuts at: 5268 5268
Size: 5392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4506 4690 1305
Size: 3201 184 2007
Fragments arranged by size:
3201 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3570
Fragments arranged by size:
3570 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3350 3709 3868 518
Size: 648 2184 359 159 2042
Fragments arranged by size:
2184 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2232 2268 2290 2808 3485 4633 4878
Size: 440 186 36 22 518 677 1148 245
Cuts at: 4878 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 518 440 245 186 36
22
NcoI C'CATG_G
Cuts at: 513 1065 2298 513
Size: 552 1233 3607
Fragments arranged by size:
3607 1233 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5392
NotI GC'GGCC_GC
Cuts at: 2839 2839
Size: 5392
NspI r_CATG'y
Cuts at: 1132 1229 2750 4295 1132
Size: 97 1521 1545 2229
Fragments arranged by size:
2229 1545 1521 97
PasI CC'CwG_GG
Cuts at: 1348 2337 1348
Size: 989 4403
PciI A'CATG_T
Cuts at: 4291 4291
Size: 5392
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2542 1108
Size: 1434 3958
PfoI T'CCnGG_A
Cuts at: 2144 2144
Size: 5392
PmeI GTTT'AAAC
Cuts at: 2696 2696
Size: 5392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 4974 5006 3461
Size: 32 1481 32 3847
Fragments arranged by size:
3847 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2463 2623 2463
Size: 160 5232
PshAI GACnn'nnGTC
Cuts at: 1613 2655 1613
Size: 1042 4350
PsiI TTA'TAA
Cuts at: 2962 2962
Size: 5392
PspOMI G'GGCC_C
Cuts at: 2072 2546 2072
Size: 474 4918
PstI C_TGCA'G
Cuts at: 830 2060 2247 2744 830
Size: 1230 187 497 3478
Fragments arranged by size:
3478 1230 497 187
PvuI CG_AT'CG
Cuts at: 2001 2130 2001
Size: 129 5263
PvuII CAG'CTG
Cuts at: 2046 2232 2268 2290 2046
Size: 186 36 22 5148
Fragments arranged by size:
5148 186 36 22
SalI G'TCGA_C
Cuts at: 1936 2734 1936
Size: 798 4594
SbfI CC_TGCA'GG
Cuts at: 2744 2744
Size: 5392
ScaI AGT'ACT
Cuts at: 2498 3640 5352 2498
Size: 1142 1712 2538
Fragments arranged by size:
2538 1712 1142
SfcI C'TryA_G
Cuts at: 826 1045 2056 2243 2438 2740 3875 4556 4747
Size: 219 1011 187 195 302 1135 681 191
Cuts at: 4747 826
Size: 1471
Fragments arranged by size:
1471 1135 1011 681 302 219 195 191
187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5392
SmaI CCC'GGG
Cuts at: 1872 2099 2222 2461 2719 1872
Size: 227 123 239 258 4545
Fragments arranged by size:
4545 258 239 227 123
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3496 4397 4659 820
Size: 197 926 1553 901 262 1553
Fragments arranged by size:
1553 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3183 493
Size: 2690 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5392
SphI G_CATG'C
Cuts at: 1132 2750 1132
Size: 1618 3774
SspI AAT'ATT
Cuts at: 5 52 3316 5
Size: 47 3264 2081
Fragments arranged by size:
3264 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5392
StyI C'CwwG_G
Cuts at: 513 1065 2298 2831 513
Size: 552 1233 533 3074
Fragments arranged by size:
3074 1233 552 533
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3419 3436 3589 3774 4219 325
Size: 1255 1839 17 153 185 445 1498
Fragments arranged by size:
1839 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 3638 5350
Size: 274 80 33 51 1443 519 1142 1712
Cuts at: 5350 96
Size: 138
Fragments arranged by size:
1712 1443 1142 519 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5352 5384 5352
Size: 32 5360
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5392
XbaI T'CTAG_A
Cuts at: 2728 2728
Size: 5392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2680 2680
Size: 5392
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5392
XmaI C'CCGG_G
Cuts at: 1870 2097 2220 2459 2717 1870
Size: 227 123 239 258 4545
Fragments arranged by size:
4545 258 239 227 123
XmnI GAAnn'nnTTC
Cuts at: 3521 3521
Size: 5392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3200 276
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI