Restriction map for
pFN21AB6929
(Circular) (Six-base) MAPSORT of: pFN21AB6929.seq Check: 4849 from: 1 to: 5326
pFN21AB6929 5326 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2373 2373
Size: 5326
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3136 278
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 1937
Size: 732 4594
Acc65I G'GTAC_C
Cuts at: 2497 2647 2497
Size: 150 5176
AclI AA'CG_TT
Cuts at: 1563 3453 3826 1563
Size: 1890 373 3063
Fragments arranged by size:
3063 1890 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3389 4773 5041 1632
Size: 1757 1384 268 1917
Fragments arranged by size:
1917 1757 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5129
AflIII A'CryG_T
Cuts at: 1203 4225 5202 1203
Size: 3022 977 1327
Fragments arranged by size:
3022 1327 977
AgeI A'CCGG_T
Cuts at: 4157 4157
Size: 5326
AhdI GACnn_n'nnGTC
Cuts at: 2232 4055 2232
Size: 1823 3503
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5326
AlwNI CAG_nnn'CTG
Cuts at: 2622 3069 4641 2622
Size: 447 1572 3307
Fragments arranged by size:
3307 1572 447
ApaI G_GGCC'C
Cuts at: 2414 2537 2414
Size: 123 5203
ApaLI G'TGCA_C
Cuts at: 2381 3383 4539 2381
Size: 1002 1156 3168
Fragments arranged by size:
3168 1156 1002
ApoI r'AATT_y
Cuts at: 1493 2635 2861 3013 5122 1493
Size: 1142 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3880 160
Size: 872 2848 1606
Fragments arranged by size:
2848 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2074 2651 1870
Size: 73 123 8 577 4545
Fragments arranged by size:
4545 577 123 73 8
BamHI G'GATC_C
Cuts at: 2061 2656 3023 2061
Size: 595 367 4364
Fragments arranged by size:
4364 595 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2088 2497 2647 4102 618
Size: 325 217 627 301 409 150 1455 1842
Fragments arranged by size:
1842 1455 627 409 325 301 217 150
BanII G_rGCy'C
Cuts at: 1391 2414 2537 2558 2577 2645 1391
Size: 1023 123 21 19 68 4072
Fragments arranged by size:
4072 1023 123 68 21 19
BbeI G_GCGC'C
Cuts at: 2092 2092
Size: 5326
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3517 3551 1903
Size: 34 1580 34 3678
Fragments arranged by size:
3678 1580 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3218 4428 3218
Size: 1210 4116
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5326
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3937 136
Size: 107 122 71 631 605 2265 1525
Fragments arranged by size:
2265 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5321 5321
Size: 5326
BlpI GC'TnA_GC
Cuts at: 2743 2743
Size: 5326
Bme1580I G_kGCm'C
Cuts at: 1790 2385 2414 2537 3387 4543 1790
Size: 595 29 123 850 1156 2573
Fragments arranged by size:
2573 1156 850 595 123 29
BmgBI CAC'GTC
Cuts at: 1318 2048 1318
Size: 730 4596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3057 4015 442
Size: 416 53 83 399 24 1640 958 1753
Fragments arranged by size:
1753 1640 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3986 1239
Size: 2747 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3451 4316 4614 3451
Size: 865 298 4163
Fragments arranged by size:
4163 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3989 882
Size: 617 2490 2219
Fragments arranged by size:
2490 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3117 493
Size: 711 1913 2702
Fragments arranged by size:
2702 1913 711
BsaBI GATnn'nnATC
Cuts at: 5320 5320
Size: 5326
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2089 2187 3133 3515
Size: 53 83 186 936 556 98 946 382
Cuts at: 3515 4150 275
Size: 635 1451
Fragments arranged by size:
1451 946 936 635 556 382 186 98
83 53
BsaWI w'CCGG_w
Cuts at: 3759 4157 4431 4578 4989 3759
Size: 398 274 147 411 4096
Fragments arranged by size:
4096 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5101
Fragments arranged by size:
5101 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 1186
Size: 1090 4236
BseYI C'CCAG_C
Cuts at: 4529 5025 4529
Size: 496 4830
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 2420 1163
Size: 573 585 99 4069
Fragments arranged by size:
4069 585 573 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2460 2776 3537 4565 1540
Size: 275 186 459 316 761 1028 2301
Fragments arranged by size:
2301 1028 761 459 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 2385 2577 3387 3472 4543 2021
Size: 364 192 810 85 1071 2804
Fragments arranged by size:
2804 1071 810 364 192 85
BsmI GAATG_Cn'
Cuts at: 2837 2930 2837
Size: 93 5233
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2385 2414 2537 2558 2577 2645
Size: 399 231 364 29 123 21 19 68
Cuts at: 2645 3387 3472 4543 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 364 231 123 85
68 29 21 19
BspEI T'CCGG_A
Cuts at: 4989 4989
Size: 5326
BspHI T'CATG_A
Cuts at: 3215 4945 3215
Size: 1730 3596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2373 2442 2681 844
Size: 1529 69 239 3489
Fragments arranged by size:
3489 1529 239 69
BsrBI CCG'CTC
Cuts at: 1394 1823 3213 1394
Size: 429 1390 3507
Fragments arranged by size:
3507 1390 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3821 3995 65
Size: 1183 2573 174 1396
Fragments arranged by size:
2573 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2455 3970 4157 1700
Size: 110 645 1515 187 2869
Fragments arranged by size:
2869 1515 645 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5326
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5326
BssSI C'ACGA_G
Cuts at: 3386 4398 3386
Size: 1012 4314
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5326
BstBI TT'CG_AA
Cuts at: 4130 4130
Size: 5326
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5326
BstXI CCAn_nnnn'nTGG
Cuts at: 2551 2551
Size: 5326
BstYI r'GATC_y
Cuts at: 1972 2061 2211 2656 3023 3408 3425 4877 4963
Size: 89 150 445 367 385 17 1452 86
Cuts at: 4963 4975 5321 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 445 385 367 346 150 89
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2037 2037
Size: 5326
BtgI C'CryG_G
Cuts at: 513 1065 2529 2550 513
Size: 552 1464 21 3289
Fragments arranged by size:
3289 1464 552 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4587
Fragments arranged by size:
4587 683 56
BtsI GCAGTG_nn'
Cuts at: 2578 2842 3636 3656 2578
Size: 264 794 20 4248
Fragments arranged by size:
4248 794 264 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5291
DraI TTT'AAA
Cuts at: 2630 2630
Size: 5326
DraIII CAC_nnn'GTG
Cuts at: 2530 2530
Size: 5326
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4333 809
Size: 507 3017 1802
Fragments arranged by size:
3017 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 2457 2773
Size: 54 1425 50 275 18 546 81 316
Cuts at: 2773 3662 5291 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 546 316 275 81 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2457 2773 1537
Size: 275 645 316 4090
Fragments arranged by size:
4090 645 316 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3256 1353
Size: 1903 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3913 4287 4433 387
Size: 1064 138 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2575 2575
Size: 5326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3389 3986 4773 5041 1239
Size: 393 1757 597 787 268 1524
Fragments arranged by size:
1757 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2041 2410 2411 2534 2041
Size: 369 1 123 4833
Fragments arranged by size:
4833 369 123 1
EcoRI G'AATT_C
Cuts at: 2635 3013 2635
Size: 378 4948
FspI TGC'GCA
Cuts at: 2609 3832 2609
Size: 1223 4103
HaeII r_GCGC'y
Cuts at: 1678 2092 2148 2431 4473 1678
Size: 414 56 283 2042 2531
Fragments arranged by size:
2531 2042 414 283 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2218 2250 3967 3999 4041
Size: 32 135 32 646 32 1717 32 42
Cuts at: 4041 4073 1373
Size: 32 2626
Fragments arranged by size:
2626 1717 646 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 2916 669
Size: 988 281 732 246 3079
Fragments arranged by size:
3079 988 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5326
HpaI GTT'AAC
Cuts at: 2916 2916
Size: 5326
KasI G'GCGC_C
Cuts at: 2088 2088
Size: 5326
KpnI G_GTAC'C
Cuts at: 2501 2651 2501
Size: 150 5176
MfeI C'AATT_G
Cuts at: 2925 2925
Size: 5326
MluI A'CGCG_T
Cuts at: 5202 5202
Size: 5326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4440 4624 1305
Size: 3135 184 2007
Fragments arranged by size:
3135 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3504
Fragments arranged by size:
3504 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 2549 2567 3284 3643 3802 518
Size: 648 1201 182 18 717 359 159 2042
Fragments arranged by size:
2042 1201 717 648 359 182 159 18
MspA1I CmG'CkG
Cuts at: 1606 2086 2619 2742 3419 4567 4812 1606
Size: 480 533 123 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 533 480 245 123
NaeI GCC'GGC
Cuts at: 2457 2457
Size: 5326
NarI GG'CG_CC
Cuts at: 2089 2089
Size: 5326
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4774
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5326
NgoMIV G'CCGG_C
Cuts at: 2455 2455
Size: 5326
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5326
NotI GC'GGCC_GC
Cuts at: 2773 2773
Size: 5326
NspI r_CATG'y
Cuts at: 1132 1229 2372 2684 4229 1132
Size: 97 1143 312 1545 2229
Fragments arranged by size:
2229 1545 1143 312 97
PasI CC'CwG_GG
Cuts at: 1348 2248 1348
Size: 900 4426
PciI A'CATG_T
Cuts at: 4225 4225
Size: 5326
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2530 1108
Size: 1422 3904
PmeI GTTT'AAAC
Cuts at: 2630 2630
Size: 5326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 4908 4940 3395
Size: 32 1481 32 3781
Fragments arranged by size:
3781 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5326
PsiI TTA'TAA
Cuts at: 2896 2896
Size: 5326
PspOMI G'GGCC_C
Cuts at: 2410 2533 2410
Size: 123 5203
PstI C_TGCA'G
Cuts at: 830 2678 830
Size: 1848 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5326
SacI G_AGCT'C
Cuts at: 2577 2577
Size: 5326
SalI G'TCGA_C
Cuts at: 1936 2668 1936
Size: 732 4594
SbfI CC_TGCA'GG
Cuts at: 2678 2678
Size: 5326
ScaI AGT'ACT
Cuts at: 3574 5286 3574
Size: 1712 3614
SfcI C'TryA_G
Cuts at: 826 1045 2674 3809 4490 4681 826
Size: 219 1629 1135 681 191 1471
Fragments arranged by size:
1629 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2090 2090
Size: 5326
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5326
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5326
SmaI CCC'GGG
Cuts at: 1872 2653 1872
Size: 781 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3430 4331 4593 820
Size: 197 926 1487 901 262 1553
Fragments arranged by size:
1553 1487 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3117 493
Size: 2624 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5326
SphI G_CATG'C
Cuts at: 1132 2684 1132
Size: 1552 3774
SspI AAT'ATT
Cuts at: 5 52 3250 5
Size: 47 3198 2081
Fragments arranged by size:
3198 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5326
StyI C'CwwG_G
Cuts at: 513 1065 2765 513
Size: 552 1700 3074
Fragments arranged by size:
3074 1700 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 3353 3370 3523 3708 4153 325
Size: 1255 456 1317 17 153 185 445 1498
Fragments arranged by size:
1498 1317 1255 456 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3572 5284 96
Size: 274 80 33 51 1443 1595 1712 138
Fragments arranged by size:
1712 1595 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2046 2078 5286 5318 2046
Size: 32 3208 32 2054
Fragments arranged by size:
3208 2054 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2138 1316
Size: 822 4504
XbaI T'CTAG_A
Cuts at: 2662 2662
Size: 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5326
XmaI C'CCGG_G
Cuts at: 1870 2651 1870
Size: 781 4545
XmnI GAAnn'nnTTC
Cuts at: 3455 3455
Size: 5326
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3134 276
Size: 53 83 186 936 1600 2468
Fragments arranged by size:
2468 1600 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI