Restriction map for
pFN21AB6924
(Circular) (Six-base) MAPSORT of: pFN21AB6924.seq Check: 3928 from: 1 to: 5386
pFN21AB6924 5386 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3196 278
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 1937
Size: 792 4594
Acc65I G'GTAC_C
Cuts at: 2707 2707
Size: 5386
AclI AA'CG_TT
Cuts at: 1563 3513 3886 1563
Size: 1950 373 3063
Fragments arranged by size:
3063 1950 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3449 4833 5101 1632
Size: 1817 1384 268 1917
Fragments arranged by size:
1917 1817 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5189
AflIII A'CryG_T
Cuts at: 1203 4285 5262 1203
Size: 3082 977 1327
Fragments arranged by size:
3082 1327 977
AgeI A'CCGG_T
Cuts at: 4217 4217
Size: 5386
AhdI GACnn_n'nnGTC
Cuts at: 4115 4115
Size: 5386
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5386
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2567 2599 2567
Size: 32 5354
AlwNI CAG_nnn'CTG
Cuts at: 3129 4701 3129
Size: 1572 3814
ApaI G_GGCC'C
Cuts at: 2307 2307
Size: 5386
ApaLI G'TGCA_C
Cuts at: 3443 4599 3443
Size: 1156 4230
ApoI r'AATT_y
Cuts at: 1493 2695 2921 3073 5182 1493
Size: 1202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3940 160
Size: 872 2908 1606
Fragments arranged by size:
2908 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
AvaI C'yCGr_G
Cuts at: 1870 1943 2224 2711 1870
Size: 73 281 487 4545
Fragments arranged by size:
4545 487 281 73
BamHI G'GATC_C
Cuts at: 2716 3083 2716
Size: 367 5019
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2427 2602 2707 4162 618
Size: 325 217 627 640 175 105 1455 1842
Fragments arranged by size:
1842 1455 640 627 325 217 175 105
BanII G_rGCy'C
Cuts at: 1391 2307 2705 1391
Size: 916 398 4072
Fragments arranged by size:
4072 916 398
BbeI G_GCGC'C
Cuts at: 2431 2431
Size: 5386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4415
BbvCI CC'TCA_GC
Cuts at: 2061 2061
Size: 5386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2495 2529 3577 3611 1903
Size: 34 50 34 474 34 1048 34 3678
Fragments arranged by size:
3678 1048 474 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2236 3278 4488 2236
Size: 1042 1210 3134
Fragments arranged by size:
3134 1210 1042
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5386
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3997 136
Size: 107 122 71 631 605 2325 1525
Fragments arranged by size:
2325 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5381 5381
Size: 5386
BlpI GC'TnA_GC
Cuts at: 2803 2803
Size: 5386
Bme1580I G_kGCm'C
Cuts at: 1790 2307 2394 2559 2607 3447 4603 1790
Size: 517 87 165 48 840 1156 2573
Fragments arranged by size:
2573 1156 840 517 165 87 48
BmgBI CAC'GTC
Cuts at: 1318 2554 1318
Size: 1236 4150
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3117 4075 442
Size: 416 53 83 399 24 1700 958 1753
Fragments arranged by size:
1753 1700 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2416 2546 4046 1239
Size: 1177 130 1500 2579
Fragments arranged by size:
2579 1500 1177 130
Bpu10I CC'TnA_GC
Cuts at: 2061 2061
Size: 5386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2158 3511 4376 4674 2158
Size: 1353 865 298 2870
Fragments arranged by size:
2870 1353 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4049 882
Size: 617 2550 2219
Fragments arranged by size:
2550 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3177 493
Size: 711 1973 2702
Fragments arranged by size:
2702 1973 711
BsaBI GATnn'nnATC
Cuts at: 5380 5380
Size: 5386
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2261 2428 3193 3575
Size: 53 83 186 936 728 167 765 382
Cuts at: 3575 4210 275
Size: 635 1451
Fragments arranged by size:
1451 936 765 728 635 382 186 167
83 53
BsaWI w'CCGG_w
Cuts at: 2230 2493 3819 4217 4491 4638 5049 2230
Size: 263 1326 398 274 147 411 2567
Fragments arranged by size:
2567 1326 411 398 274 263 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2567 2597 1178
Size: 30 165 30 1164 30 3967
Fragments arranged by size:
3967 1164 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2061 2543 2546 2660 1186
Size: 875 482 3 114 3912
Fragments arranged by size:
3912 875 482 114 3
BseYI C'CCAG_C
Cuts at: 2598 4589 5085 2598
Size: 1991 496 2899
Fragments arranged by size:
2899 1991 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4813
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2123 2494 2836 3597 4625 1540
Size: 275 186 122 371 342 761 1028 2301
Fragments arranged by size:
2301 1028 761 371 342 275 186 122
BsiHKAI G_wGCw'C
Cuts at: 3447 3532 4603 3447
Size: 85 1071 4230
Fragments arranged by size:
4230 1071 85
BsmI GAATG_Cn'
Cuts at: 2081 2897 2990 2081
Size: 816 93 4477
Fragments arranged by size:
4477 816 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2394 2559 2607 2705 3447 3532
Size: 399 517 87 165 48 98 742 85
Cuts at: 3532 4603 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 165 98 87
85 48
BspEI T'CCGG_A
Cuts at: 5049 5049
Size: 5386
BspHI T'CATG_A
Cuts at: 2544 3275 5005 2544
Size: 731 1730 2925
Fragments arranged by size:
2925 1730 731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2741 844
Size: 1897 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3273 1394
Size: 429 1450 3507
Fragments arranged by size:
3507 1450 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3881 4055 65
Size: 1183 2633 174 1396
Fragments arranged by size:
2633 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4030 4217 1700
Size: 110 2220 187 2869
Fragments arranged by size:
2869 2220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5386
BssSI C'ACGA_G
Cuts at: 3446 4458 3446
Size: 1012 4374
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2281 794
Size: 1487 3899
BstBI TT'CG_AA
Cuts at: 4190 4190
Size: 5386
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5386
BstYI r'GATC_y
Cuts at: 1972 2716 3083 3468 3485 4937 5023 5035 5381
Size: 744 367 385 17 1452 86 12 346
Cuts at: 5381 1972
Size: 1977
Fragments arranged by size:
1977 1452 744 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2162 2665 513
Size: 552 1097 503 3234
Fragments arranged by size:
3234 1097 552 503
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2443 487
Size: 683 56 1217 3430
Fragments arranged by size:
3430 1217 683 56
BtsI GCAGTG_nn'
Cuts at: 2902 3696 3716 2902
Size: 794 20 4572
Fragments arranged by size:
4572 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5351
DraI TTT'AAA
Cuts at: 2690 2690
Size: 5386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4393 809
Size: 507 3077 1802
Fragments arranged by size:
3077 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2833 3722
Size: 54 1425 50 275 18 531 472 889
Cuts at: 3722 5351 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 531 472 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2833 1537
Size: 275 1021 4090
Fragments arranged by size:
4090 1021 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3316 1353
Size: 1963 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 2173 2210 2277 3973 4347
Size: 1064 138 498 86 37 67 1696 374
Cuts at: 4347 4493 387
Size: 146 1280
Fragments arranged by size:
1696 1280 1064 498 374 146 138 86
67 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2416 2546 3449 4046 4833 5101 1239
Size: 393 784 130 903 597 787 268 1524
Fragments arranged by size:
1524 903 787 784 597 393 268 130
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 5386
EcoO109I rG'GnC_Cy
Cuts at: 2499 2499
Size: 5386
EcoRI G'AATT_C
Cuts at: 2695 3073 2695
Size: 378 5008
FspI TGC'GCA
Cuts at: 2636 3892 2636
Size: 1256 4130
HaeII r_GCGC'y
Cuts at: 1678 2431 4533 1678
Size: 753 2102 2531
Fragments arranged by size:
2531 2102 753
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4027 4059 4101 4133 1373
Size: 32 135 32 2455 32 42 32 2626
Fragments arranged by size:
2626 2455 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2730 2976 669
Size: 988 281 792 246 3079
Fragments arranged by size:
3079 988 792 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5386
HpaI GTT'AAC
Cuts at: 2976 2976
Size: 5386
KasI G'GCGC_C
Cuts at: 2427 2427
Size: 5386
KpnI G_GTAC'C
Cuts at: 2711 2711
Size: 5386
MfeI C'AATT_G
Cuts at: 2985 2985
Size: 5386
MluI A'CGCG_T
Cuts at: 5262 5262
Size: 5386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 4500 4684 1305
Size: 798 2397 184 2007
Fragments arranged by size:
2397 2007 798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 10
Size: 54 1425 343 531 3033
Fragments arranged by size:
3033 1425 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2543 3344 3703 3862 518
Size: 648 1035 342 801 359 159 2042
Fragments arranged by size:
2042 1035 801 648 359 342 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2164 2598 2802 3479 4627 4872 1606
Size: 459 99 434 204 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 459 434 245 204 99
NarI GG'CG_CC
Cuts at: 2428 2428
Size: 5386
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4834
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5386
NotI GC'GGCC_GC
Cuts at: 2833 2833
Size: 5386
NspI r_CATG'y
Cuts at: 1132 1229 2284 2359 2744 4289 1132
Size: 97 1055 75 385 1545 2229
Fragments arranged by size:
2229 1545 1055 385 97 75
PasI CC'CwG_GG
Cuts at: 1348 2091 1348
Size: 743 4643
PciI A'CATG_T
Cuts at: 4285 4285
Size: 5386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5386
PfoI T'CCnGG_A
Cuts at: 2142 2142
Size: 5386
PmeI GTTT'AAAC
Cuts at: 2690 2690
Size: 5386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2567 2599 3455 3487 4968 5000 2567
Size: 32 856 32 1481 32 2953
Fragments arranged by size:
2953 1481 856 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2499 2499
Size: 5386
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5386
PsiI TTA'TAA
Cuts at: 2956 2956
Size: 5386
PspOMI G'GGCC_C
Cuts at: 2303 2303
Size: 5386
PstI C_TGCA'G
Cuts at: 830 2070 2738 830
Size: 1240 668 3478
Fragments arranged by size:
3478 1240 668
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5386
PvuII CAG'CTG
Cuts at: 2065 2065
Size: 5386
SacII CC_GC'GG
Cuts at: 2165 2165
Size: 5386
SalI G'TCGA_C
Cuts at: 1936 2728 1936
Size: 792 4594
SbfI CC_TGCA'GG
Cuts at: 2738 2738
Size: 5386
ScaI AGT'ACT
Cuts at: 3634 5346 3634
Size: 1712 3674
SfcI C'TryA_G
Cuts at: 826 1045 2066 2350 2734 3869 4550 4741 826
Size: 219 1021 284 384 1135 681 191 1471
Fragments arranged by size:
1471 1135 1021 681 384 284 219 191
SfoI GGC'GCC
Cuts at: 2429 2429
Size: 5386
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5386
SmaI CCC'GGG
Cuts at: 1872 2226 2713 1872
Size: 354 487 4545
Fragments arranged by size:
4545 487 354
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2173 3490 4391 4653 820
Size: 197 926 230 1317 901 262 1553
Fragments arranged by size:
1553 1317 926 901 262 230 197
SnaBI TAC'GTA
Cuts at: 493 3177 493
Size: 2684 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5386
SphI G_CATG'C
Cuts at: 1132 2359 2744 1132
Size: 1227 385 3774
Fragments arranged by size:
3774 1227 385
SspI AAT'ATT
Cuts at: 5 52 3310 5
Size: 47 3258 2081
Fragments arranged by size:
3258 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5386
StyI C'CwwG_G
Cuts at: 513 1065 2015 2825 513
Size: 552 950 810 3074
Fragments arranged by size:
3074 950 810 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2590 3413 3430 3583 3768 4213 325
Size: 1255 1010 823 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1010 823 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3632 5344 96
Size: 274 80 33 51 1443 1655 1712 138
Fragments arranged by size:
1712 1655 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2220 2252 5346 5378 2220
Size: 32 3094 32 2228
Fragments arranged by size:
3094 2228 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5386
XbaI T'CTAG_A
Cuts at: 2722 2722
Size: 5386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2194 2194
Size: 5386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5386
XmaI C'CCGG_G
Cuts at: 1870 2224 2711 1870
Size: 354 487 4545
Fragments arranged by size:
4545 487 354
XmnI GAAnn'nnTTC
Cuts at: 3515 3515
Size: 5386
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3194 276
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI