Restriction map for
pFN21AB6922
(Circular) (Six-base) MAPSORT of: pFN21AB6922.seq Check: 5458 from: 1 to: 5386
pFN21AB6922 5386 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3196 278
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 1937
Size: 792 4594
Acc65I G'GTAC_C
Cuts at: 2707 2707
Size: 5386
AclI AA'CG_TT
Cuts at: 1563 3513 3886 1563
Size: 1950 373 3063
Fragments arranged by size:
3063 1950 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2320 3449 4833 5101 1632
Size: 688 1129 1384 268 1917
Fragments arranged by size:
1917 1384 1129 688 268
AfeI AGC'GCT
Cuts at: 2654 2654
Size: 5386
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5189
AflIII A'CryG_T
Cuts at: 1203 4285 5262 1203
Size: 3082 977 1327
Fragments arranged by size:
3082 1327 977
AgeI A'CCGG_T
Cuts at: 4217 4217
Size: 5386
AhdI GACnn_n'nnGTC
Cuts at: 4115 4115
Size: 5386
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2531 1184
Size: 1347 4039
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2087 2119 2087
Size: 32 5354
AlwNI CAG_nnn'CTG
Cuts at: 2423 3129 4701 2423
Size: 706 1572 3108
Fragments arranged by size:
3108 1572 706
ApaI G_GGCC'C
Cuts at: 2082 2082
Size: 5386
ApaLI G'TGCA_C
Cuts at: 3443 4599 3443
Size: 1156 4230
ApoI r'AATT_y
Cuts at: 1493 2695 2921 3073 5182 1493
Size: 1202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3940 160
Size: 872 2908 1606
Fragments arranged by size:
2908 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
AvaI C'yCGr_G
Cuts at: 1870 1943 2171 2432 2711 1870
Size: 73 228 261 279 4545
Fragments arranged by size:
4545 279 261 228 73
BamHI G'GATC_C
Cuts at: 2716 3083 2716
Size: 367 5019
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2677 2707 4162 618
Size: 325 217 627 890 30 1455 1842
Fragments arranged by size:
1842 1455 890 627 325 217 30
BanII G_rGCy'C
Cuts at: 1391 2082 2234 2433 2705 1391
Size: 691 152 199 272 4072
Fragments arranged by size:
4072 691 272 199 152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3577 3611 1903
Size: 34 1640 34 3678
Fragments arranged by size:
3678 1640 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3278 4488 3278
Size: 1210 4176
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5386
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2021 2238
Size: 107 122 71 631 605 337 12 217
Cuts at: 2238 2540 3997 136
Size: 302 1457 1525
Fragments arranged by size:
1525 1457 631 605 337 302 217 122
107 71 12
BglII A'GATC_T
Cuts at: 5381 5381
Size: 5386
BlpI GC'TnA_GC
Cuts at: 2803 2803
Size: 5386
Bme1580I G_kGCm'C
Cuts at: 1790 2082 2682 3447 4603 1790
Size: 292 600 765 1156 2573
Fragments arranged by size:
2573 1156 765 600 292
BmgBI CAC'GTC
Cuts at: 1318 2212 1318
Size: 894 4492
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3117 4075 442
Size: 416 53 83 399 24 1700 958 1753
Fragments arranged by size:
1753 1700 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2372 2519 4046 1239
Size: 1007 126 147 1527 2579
Fragments arranged by size:
2579 1527 1007 147 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 3511 4376 4674 2633
Size: 878 865 298 3345
Fragments arranged by size:
3345 878 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2168 2474 4049 882
Size: 617 669 306 1575 2219
Fragments arranged by size:
2219 1575 669 617 306
BsaAI yAC'GTr
Cuts at: 493 1204 2546 3177 493
Size: 711 1342 631 2702
Fragments arranged by size:
2702 1342 711 631
BsaBI GATnn'nnATC
Cuts at: 5380 5380
Size: 5386
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3193 3575 4210 275
Size: 53 83 186 936 1660 382 635 1451
Fragments arranged by size:
1660 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3819 4217 4491 4638 5049 3819
Size: 398 274 147 411 4156
Fragments arranged by size:
4156 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5161
Fragments arranged by size:
5161 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 2450 1186
Size: 1228 36 4122
Fragments arranged by size:
4122 1228 36
BseYI C'CCAG_C
Cuts at: 4589 5085 4589
Size: 496 4890
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2626 1163
Size: 573 890 3923
Fragments arranged by size:
3923 890 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2836 3597 4625 1540
Size: 275 186 15 820 761 1028 2301
Fragments arranged by size:
2301 1028 820 761 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 3447 3532 4603 3447
Size: 85 1071 4230
Fragments arranged by size:
4230 1071 85
BsmI GAATG_Cn'
Cuts at: 2897 2990 2897
Size: 93 5293
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2234 2433 2682 2705 3447 3532
Size: 399 292 152 199 249 23 742 85
Cuts at: 3532 4603 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 292 249 199 152
85 23
BspEI T'CCGG_A
Cuts at: 5049 5049
Size: 5386
BspHI T'CATG_A
Cuts at: 3275 5005 3275
Size: 1730 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2565 2626 2741 844
Size: 1721 61 115 3489
Fragments arranged by size:
3489 1721 115 61
BsrBI CCG'CTC
Cuts at: 1394 1823 2578 3273 1394
Size: 429 755 695 3507
Fragments arranged by size:
3507 755 695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3881 4055 65
Size: 1183 2633 174 1396
Fragments arranged by size:
2633 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 2119 2155 4030 4217 1700
Size: 110 205 104 36 1875 187 2869
Fragments arranged by size:
2869 1875 205 187 110 104 36
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5386
BssSI C'ACGA_G
Cuts at: 3446 4458 3446
Size: 1012 4374
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5386
BstBI TT'CG_AA
Cuts at: 4190 4190
Size: 5386
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5386
BstYI r'GATC_y
Cuts at: 1972 2716 3083 3468 3485 4937 5023 5035 5381
Size: 744 367 385 17 1452 86 12 346
Cuts at: 5381 1972
Size: 1977
Fragments arranged by size:
1977 1452 744 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2235 2235
Size: 5386
BtgI C'CryG_G
Cuts at: 513 1065 2682 513
Size: 552 1617 3217
Fragments arranged by size:
3217 1617 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4647
Fragments arranged by size:
4647 683 56
BtsI GCAGTG_nn'
Cuts at: 2141 2902 3696 3716 2141
Size: 761 794 20 3811
Fragments arranged by size:
3811 794 761 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5351
DraI TTT'AAA
Cuts at: 2690 2690
Size: 5386
DraIII CAC_nnn'GTG
Cuts at: 2287 2287
Size: 5386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2402 4393 809
Size: 507 1086 1991 1802
Fragments arranged by size:
1991 1802 1086 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2157 2833
Size: 54 1425 50 275 18 183 144 676
Cuts at: 2833 3722 5351 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 676 275 183 144 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2833 1537
Size: 275 201 820 4090
Fragments arranged by size:
4090 820 275 201
EarI CTCTTCn'nnn_
Cuts at: 1353 3316 1353
Size: 1963 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3973 4347 4493 387
Size: 1064 138 2384 374 146 1280
Fragments arranged by size:
2384 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2320 2372 2519 3449 4046 4833
Size: 393 614 74 52 147 930 597 787
Cuts at: 4833 5101 1239
Size: 268 1524
Fragments arranged by size:
1524 930 787 614 597 393 268 147
74 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2561 2561
Size: 5386
EcoO109I rG'GnC_Cy
Cuts at: 2078 2079 2078
Size: 1 5385
EcoRI G'AATT_C
Cuts at: 2695 3073 2695
Size: 378 5008
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2674 2338
Size: 336 5050
FspI TGC'GCA
Cuts at: 3892 3892
Size: 5386
HaeII r_GCGC'y
Cuts at: 1678 2656 2674 4533 1678
Size: 978 18 1859 2531
Fragments arranged by size:
2531 1859 978 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4027 4059 4101 4133 1373
Size: 32 135 32 2455 32 42 32 2626
Fragments arranged by size:
2626 2455 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2584 2602 2730 2976 669
Size: 988 281 646 18 128 246 3079
Fragments arranged by size:
3079 988 646 281 246 128 18
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5386
HpaI GTT'AAC
Cuts at: 2976 2976
Size: 5386
KpnI G_GTAC'C
Cuts at: 2711 2711
Size: 5386
MfeI C'AATT_G
Cuts at: 2985 2985
Size: 5386
MluI A'CGCG_T
Cuts at: 5262 5262
Size: 5386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4500 4684 1305
Size: 3195 184 2007
Fragments arranged by size:
3195 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3564
Fragments arranged by size:
3564 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2513 3344 3703 3862 518
Size: 648 1347 831 359 159 2042
Fragments arranged by size:
2042 1347 831 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2597 2802 3479 4627 4872 1606
Size: 991 205 677 1148 245 2120
Fragments arranged by size:
2120 1148 991 677 245 205
NaeI GCC'GGC
Cuts at: 2017 2121 2157 2017
Size: 104 36 5246
Fragments arranged by size:
5246 104 36
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4834
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5386
NgoMIV G'CCGG_C
Cuts at: 2015 2119 2155 2015
Size: 104 36 5246
Fragments arranged by size:
5246 104 36
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5386
NotI GC'GGCC_GC
Cuts at: 2833 2833
Size: 5386
NspI r_CATG'y
Cuts at: 1132 1229 2744 4289 1132
Size: 97 1515 1545 2229
Fragments arranged by size:
2229 1545 1515 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5386
PciI A'CATG_T
Cuts at: 4285 4285
Size: 5386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5386
PfoI T'CCnGG_A
Cuts at: 2091 2388 2661 2091
Size: 297 273 4816
Fragments arranged by size:
4816 297 273
PmeI GTTT'AAAC
Cuts at: 2690 2690
Size: 5386
PmlI CAC'GTG
Cuts at: 2546 2546
Size: 5386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2125 3455 3487 4968 5000 2093
Size: 32 1330 32 1481 32 2479
Fragments arranged by size:
2479 1481 1330 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5386
PsiI TTA'TAA
Cuts at: 2956 2956
Size: 5386
PspOMI G'GGCC_C
Cuts at: 2078 2078
Size: 5386
PstI C_TGCA'G
Cuts at: 830 2637 2738 830
Size: 1807 101 3478
Fragments arranged by size:
3478 1807 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5386
PvuII CAG'CTG
Cuts at: 2597 2597
Size: 5386
SalI G'TCGA_C
Cuts at: 1936 2728 1936
Size: 792 4594
SbfI CC_TGCA'GG
Cuts at: 2738 2738
Size: 5386
ScaI AGT'ACT
Cuts at: 3634 5346 3634
Size: 1712 3674
SexAI A'CCwGG_T
Cuts at: 2184 2184
Size: 5386
SfcI C'TryA_G
Cuts at: 826 1045 2633 2734 3869 4550 4741 826
Size: 219 1588 101 1135 681 191 1471
Fragments arranged by size:
1588 1471 1135 681 219 191 101
SfiI GGCCn_nnn'nGGCC
Cuts at: 2021 2021
Size: 5386
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5386
SmaI CCC'GGG
Cuts at: 1872 2713 1872
Size: 841 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 2648 3490 4391 4653 820
Size: 197 926 228 477 842 901 262 1553
Fragments arranged by size:
1553 926 901 842 477 262 228 197
SnaBI TAC'GTA
Cuts at: 493 3177 493
Size: 2684 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5386
SphI G_CATG'C
Cuts at: 1132 2744 1132
Size: 1612 3774
SspI AAT'ATT
Cuts at: 5 52 3310 5
Size: 47 3258 2081
Fragments arranged by size:
3258 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5386
StyI C'CwwG_G
Cuts at: 513 1065 2825 513
Size: 552 1760 3074
Fragments arranged by size:
3074 1760 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 3413 3430 3583 3768 4213 325
Size: 1255 612 1221 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1221 612 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3632 5344 96
Size: 274 80 33 51 1443 1655 1712 138
Fragments arranged by size:
1712 1655 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 5346 5378 2196
Size: 32 3118 32 2204
Fragments arranged by size:
3118 2204 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5386
XbaI T'CTAG_A
Cuts at: 2722 2722
Size: 5386
XhoI C'TCGA_G
Cuts at: 1943 2171 1943
Size: 228 5158
XmaI C'CCGG_G
Cuts at: 1870 2711 1870
Size: 841 4545
XmnI GAAnn'nnTTC
Cuts at: 3515 3515
Size: 5386
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3194 276
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfoI SrfI
SwaI XcmI