Restriction map for
pFN21AB6912
(Circular) (Six-base) MAPSORT of: pFN21AB6912.seq Check: 4590 from: 1 to: 5359
pFN21AB6912 5359 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2178 3169 278
Size: 53 83 186 936 642 991 2468
Fragments arranged by size:
2468 991 936 642 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2702 1937
Size: 765 4594
Acc65I G'GTAC_C
Cuts at: 2680 2680
Size: 5359
AclI AA'CG_TT
Cuts at: 1563 3486 3859 1563
Size: 1923 373 3063
Fragments arranged by size:
3063 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3422 4806 5074 1632
Size: 1790 1384 268 1917
Fragments arranged by size:
1917 1790 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5162
AflIII A'CryG_T
Cuts at: 1203 2401 4258 5235 1203
Size: 1198 1857 977 1327
Fragments arranged by size:
1857 1327 1198 977
AgeI A'CCGG_T
Cuts at: 4190 4190
Size: 5359
AhdI GACnn_n'nnGTC
Cuts at: 4088 4088
Size: 5359
AleI CACnn'nnGTG
Cuts at: 1166 2300 1166
Size: 1134 4225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5359
AlwNI CAG_nnn'CTG
Cuts at: 3102 4674 3102
Size: 1572 3787
ApaLI G'TGCA_C
Cuts at: 3416 4572 3416
Size: 1156 4203
ApoI r'AATT_y
Cuts at: 1493 2668 2894 3046 5155 1493
Size: 1175 226 152 2109 1697
Fragments arranged by size:
2109 1697 1175 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3913 160
Size: 872 2881 1606
Fragments arranged by size:
2881 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 1870
Size: 73 741 4545
Fragments arranged by size:
4545 741 73
BamHI G'GATC_C
Cuts at: 2689 3056 2689
Size: 367 4992
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2289 2428 2470 2648 2680
Size: 325 217 627 502 139 42 178 32
Cuts at: 2680 4135 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 502 325 217 178 139
42 32
BanII G_rGCy'C
Cuts at: 1391 2571 2678 1391
Size: 1180 107 4072
Fragments arranged by size:
4072 1180 107
BbeI G_GCGC'C
Cuts at: 2293 2474 2293
Size: 181 5178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2378 2484 2634 928
Size: 971 479 106 150 3653
Fragments arranged by size:
3653 971 479 150 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2638 2672 3550 3584 1903
Size: 34 701 34 878 34 3678
Fragments arranged by size:
3678 878 701 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2583 3251 4461 2583
Size: 668 1210 3481
Fragments arranged by size:
3481 1210 668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3970 136
Size: 107 122 71 631 605 2298 1525
Fragments arranged by size:
2298 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5354 5354
Size: 5359
BlpI GC'TnA_GC
Cuts at: 2776 2776
Size: 5359
Bme1580I G_kGCm'C
Cuts at: 1790 2433 2651 3420 4576 1790
Size: 643 218 769 1156 2573
Fragments arranged by size:
2573 1156 769 643 218
BmgBI CAC'GTC
Cuts at: 1318 2404 1318
Size: 1086 4273
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3090 4048 442
Size: 416 53 83 399 24 1673 958 1753
Fragments arranged by size:
1753 1673 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4019 1239
Size: 2780 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3484 4349 4647 3484
Size: 865 298 4196
Fragments arranged by size:
4196 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2114 4022 882
Size: 617 615 1908 2219
Fragments arranged by size:
2219 1908 617 615
BsaAI yAC'GTr
Cuts at: 493 1204 3150 493
Size: 711 1946 2702
Fragments arranged by size:
2702 1946 711
BsaBI GATnn'nnATC
Cuts at: 5353 5353
Size: 5359
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2146 2175 2290 2471
Size: 53 83 186 936 613 29 115 181
Cuts at: 2471 3166 3548 4183 275
Size: 695 382 635 1451
Fragments arranged by size:
1451 936 695 635 613 382 186 181
115 83 53 29
BsaWI w'CCGG_w
Cuts at: 3792 4190 4464 4611 5022 3792
Size: 398 274 147 411 4129
Fragments arranged by size:
4129 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5134
Fragments arranged by size:
5134 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 2459 2501 2579 1186
Size: 1270 3 42 78 3966
Fragments arranged by size:
3966 1270 78 42 3
BseYI C'CCAG_C
Cuts at: 2097 4562 5058 2097
Size: 2465 496 2398
Fragments arranged by size:
2465 2398 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2603 1163
Size: 573 867 3919
Fragments arranged by size:
3919 867 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2135 2212 2809 3570 4598 1540
Size: 275 186 134 77 597 761 1028 2301
Fragments arranged by size:
2301 1028 761 597 275 186 134 77
BsiHKAI G_wGCw'C
Cuts at: 3420 3505 4576 3420
Size: 85 1071 4203
Fragments arranged by size:
4203 1071 85
BsmI GAATG_Cn'
Cuts at: 2870 2963 2870
Size: 93 5266
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2433 2571 2651 2678 3420 3505 4576
Size: 399 643 138 80 27 742 85 1071
Cuts at: 4576 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 643 399 138 85 80
27
BspEI T'CCGG_A
Cuts at: 5022 5022
Size: 5359
BspHI T'CATG_A
Cuts at: 3248 4978 3248
Size: 1730 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2714 844
Size: 1870 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2221 3246 1394
Size: 429 398 1025 3507
Fragments arranged by size:
3507 1025 429 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 3854 4028 65
Size: 1183 2606 174 1396
Fragments arranged by size:
2606 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 2155 2467 4003 4190 1700
Size: 110 338 7 312 1536 187 2869
Fragments arranged by size:
2869 1536 338 312 187 110 7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5359
BssHII G'CGCG_C
Cuts at: 1925 2059 1925
Size: 134 5225
BssSI C'ACGA_G
Cuts at: 2438 3419 4431 2438
Size: 981 1012 3366
Fragments arranged by size:
3366 1012 981
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5359
BstBI TT'CG_AA
Cuts at: 4163 4163
Size: 5359
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5359
BstYI r'GATC_y
Cuts at: 1972 2689 3056 3441 3458 4910 4996 5008 5354
Size: 717 367 385 17 1452 86 12 346
Cuts at: 5354 1972
Size: 1977
Fragments arranged by size:
1977 1452 717 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2027 2335 513
Size: 552 939 23 308 3537
Fragments arranged by size:
3537 939 552 308 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4620
Fragments arranged by size:
4620 683 56
BtsI GCAGTG_nn'
Cuts at: 2875 3669 3689 2875
Size: 794 20 4545
Fragments arranged by size:
4545 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5324
DraI TTT'AAA
Cuts at: 2663 2663
Size: 5359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4366 809
Size: 507 3050 1802
Fragments arranged by size:
3050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2132 2209 2806
Size: 54 1425 50 275 18 302 77 597
Cuts at: 2806 3695 5324 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 597 302 275 77 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2132 2209 2806 1537
Size: 275 320 77 597 4090
Fragments arranged by size:
4090 597 320 275 77
EarI CTCTTCn'nnn_
Cuts at: 1353 2228 3289 1353
Size: 875 1061 3423
Fragments arranged by size:
3423 1061 875
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2168 3946 4320 4466 387
Size: 1064 138 579 1778 374 146 1280
Fragments arranged by size:
1778 1280 1064 579 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3422 4019 4806 5074 1239
Size: 393 1790 597 787 268 1524
Fragments arranged by size:
1790 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2668 3046 2668
Size: 378 4981
FspI TGC'GCA
Cuts at: 3865 3865
Size: 5359
HaeII r_GCGC'y
Cuts at: 1678 2293 2474 4506 1678
Size: 615 181 2032 2531
Fragments arranged by size:
2531 2032 615 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4000 4032 4074 4106 1373
Size: 32 135 32 2428 32 42 32 2626
Fragments arranged by size:
2626 2428 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 2949 669
Size: 988 281 765 246 3079
Fragments arranged by size:
3079 988 765 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5359
HpaI GTT'AAC
Cuts at: 2949 2949
Size: 5359
KasI G'GCGC_C
Cuts at: 2289 2470 2289
Size: 181 5178
KpnI G_GTAC'C
Cuts at: 2684 2684
Size: 5359
MfeI C'AATT_G
Cuts at: 2958 2958
Size: 5359
MluI A'CGCG_T
Cuts at: 5235 5235
Size: 5359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4473 4657 1305
Size: 3168 184 2007
Fragments arranged by size:
3168 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3537
Fragments arranged by size:
3537 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 3317 3676 3835 518
Size: 648 1134 1017 359 159 2042
Fragments arranged by size:
2042 1134 1017 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2203 2775 3452 4600 4845 1606
Size: 423 174 572 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 572 423 245 174
NaeI GCC'GGC
Cuts at: 2150 2469 2150
Size: 319 5040
NarI GG'CG_CC
Cuts at: 2290 2471 2290
Size: 181 5178
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3868
Fragments arranged by size:
3868 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5359
NgoMIV G'CCGG_C
Cuts at: 2148 2467 2148
Size: 319 5040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5359
NotI GC'GGCC_GC
Cuts at: 2806 2806
Size: 5359
NspI r_CATG'y
Cuts at: 1132 1229 2717 4262 1132
Size: 97 1488 1545 2229
Fragments arranged by size:
2229 1545 1488 97
PasI CC'CwG_GG
Cuts at: 1348 2604 1348
Size: 1256 4103
PciI A'CATG_T
Cuts at: 4258 4258
Size: 5359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5359
PmeI GTTT'AAAC
Cuts at: 2663 2663
Size: 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 4941 4973 3428
Size: 32 1481 32 3814
Fragments arranged by size:
3814 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5359
PsiI TTA'TAA
Cuts at: 2929 2929
Size: 5359
PstI C_TGCA'G
Cuts at: 830 2711 830
Size: 1881 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5359
PvuII CAG'CTG
Cuts at: 2203 2203
Size: 5359
RsrII CG'GwC_CG
Cuts at: 2158 2158
Size: 5359
SacII CC_GC'GG
Cuts at: 2030 2030
Size: 5359
SalI G'TCGA_C
Cuts at: 1936 2701 1936
Size: 765 4594
SapI GCTCTTCn'nnn_
Cuts at: 2228 2228
Size: 5359
SbfI CC_TGCA'GG
Cuts at: 2711 2711
Size: 5359
ScaI AGT'ACT
Cuts at: 3607 5319 3607
Size: 1712 3647
SfcI C'TryA_G
Cuts at: 826 1045 2707 3842 4523 4714 826
Size: 219 1662 1135 681 191 1471
Fragments arranged by size:
1662 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2291 2472 2291
Size: 181 5178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
SgrAI Cr'CCGG_yG
Cuts at: 1700 2467 1700
Size: 767 4592
SmaI CCC'GGG
Cuts at: 1872 2686 1872
Size: 814 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3463 4364 4626 820
Size: 197 926 1520 901 262 1553
Fragments arranged by size:
1553 1520 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3150 493
Size: 2657 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5359
SphI G_CATG'C
Cuts at: 1132 2717 1132
Size: 1585 3774
SspI AAT'ATT
Cuts at: 5 52 3283 5
Size: 47 3231 2081
Fragments arranged by size:
3231 2081 47
StuI AGG'CCT
Cuts at: 1517 2345 2450 1517
Size: 828 105 4426
Fragments arranged by size:
4426 828 105
StyI C'CwwG_G
Cuts at: 513 1065 2004 2643 2798 513
Size: 552 939 639 155 3074
Fragments arranged by size:
3074 939 639 552 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3386 3403 3556 3741 4186 325
Size: 1255 1806 17 153 185 445 1498
Fragments arranged by size:
1806 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3605 5317 96
Size: 274 80 33 51 1443 1628 1712 138
Fragments arranged by size:
1712 1628 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5319 5351 5319
Size: 32 5327
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5359
XbaI T'CTAG_A
Cuts at: 2695 2695
Size: 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5359
XmaI C'CCGG_G
Cuts at: 1870 2684 1870
Size: 814 4545
XmnI GAAnn'nnTTC
Cuts at: 3488 3488
Size: 5359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2176 3167 276
Size: 53 83 186 936 642 991 2468
Fragments arranged by size:
2468 991 936 642 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI SacI SanDI SexAI SfiI SrfI SwaI XcmI