Restriction map for
pFN21AB6911
(Circular) (Six-base) MAPSORT of: pFN21AB6911.seq Check: 3293 from: 1 to: 5320
pFN21AB6911 5320 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2435 2435
Size: 5320
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2184 3130 278
Size: 53 83 186 936 648 946 2468
Fragments arranged by size:
2468 946 936 648 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2663 1937
Size: 726 4594
Acc65I G'GTAC_C
Cuts at: 2641 2641
Size: 5320
AclI AA'CG_TT
Cuts at: 1563 3447 3820 1563
Size: 1884 373 3063
Fragments arranged by size:
3063 1884 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2624 3383 4767 5035 1632
Size: 992 759 1384 268 1917
Fragments arranged by size:
1917 1384 992 759 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5123
AflIII A'CryG_T
Cuts at: 1203 4219 5196 1203
Size: 3016 977 1327
Fragments arranged by size:
3016 1327 977
AgeI A'CCGG_T
Cuts at: 4151 4151
Size: 5320
AhdI GACnn_n'nnGTC
Cuts at: 4049 4049
Size: 5320
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5320
AlwNI CAG_nnn'CTG
Cuts at: 3063 4635 3063
Size: 1572 3748
ApaLI G'TGCA_C
Cuts at: 3377 4533 3377
Size: 1156 4164
ApoI r'AATT_y
Cuts at: 1493 2629 2855 3007 5116 1493
Size: 1136 226 152 2109 1697
Fragments arranged by size:
2109 1697 1136 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3874 160
Size: 872 2842 1606
Fragments arranged by size:
2842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
AvaI C'yCGr_G
Cuts at: 1870 1943 2645 1870
Size: 73 702 4545
Fragments arranged by size:
4545 702 73
BamHI G'GATC_C
Cuts at: 2091 2479 2650 3017 2091
Size: 388 171 367 4394
Fragments arranged by size:
4394 388 367 171
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2423 2641 4096 618
Size: 325 217 627 636 218 1455 1842
Fragments arranged by size:
1842 1455 636 627 325 218 217
BanII G_rGCy'C
Cuts at: 1391 2172 2472 2639 1391
Size: 781 300 167 4072
Fragments arranged by size:
4072 781 300 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2354 928
Size: 971 455 3894
Fragments arranged by size:
3894 971 455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3511 3545 1903
Size: 34 1574 34 3678
Fragments arranged by size:
3678 1574 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3212 4422 3212
Size: 1210 4110
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5320
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 3931 136
Size: 107 122 71 631 605 367 1892 1525
Fragments arranged by size:
1892 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 2127 5315 2127
Size: 3188 2132
BlpI GC'TnA_GC
Cuts at: 2737 2737
Size: 5320
Bme1580I G_kGCm'C
Cuts at: 1790 3381 4537 1790
Size: 1591 1156 2573
Fragments arranged by size:
2573 1591 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5320
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3051 4009 442
Size: 416 53 83 399 24 1634 958 1753
Fragments arranged by size:
1753 1634 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3980 1239
Size: 2741 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 3445 4310 4608 2189
Size: 1256 865 298 2901
Fragments arranged by size:
2901 1256 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3983 882
Size: 617 2484 2219
Fragments arranged by size:
2484 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3111 493
Size: 711 1907 2702
Fragments arranged by size:
2702 1907 711
BsaBI GATnn'nnATC
Cuts at: 2484 5314 2484
Size: 2830 2490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2181 3127 3509 4144
Size: 53 83 186 936 648 946 382 635
Cuts at: 4144 275
Size: 1451
Fragments arranged by size:
1451 946 936 648 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2482 3753 4151 4425 4572 4983 2482
Size: 1271 398 274 147 411 2819
Fragments arranged by size:
2819 1271 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2019 2049 2547 2577 1178
Size: 30 165 30 616 30 498 30 3921
Fragments arranged by size:
3921 616 498 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5320
BseYI C'CCAG_C
Cuts at: 2196 4523 5019 2196
Size: 2327 496 2497
Fragments arranged by size:
2497 2327 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4747
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2770 3531 4559 1540
Size: 275 186 769 761 1028 2301
Fragments arranged by size:
2301 1028 769 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3381 3466 4537 3381
Size: 85 1071 4164
Fragments arranged by size:
4164 1071 85
BsmI GAATG_Cn'
Cuts at: 2527 2831 2924 2527
Size: 304 93 4923
Fragments arranged by size:
4923 304 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2472 2639 3381 3466 4537 1391
Size: 399 382 300 167 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 300 167 85
BspEI T'CCGG_A
Cuts at: 2482 4983 2482
Size: 2501 2819
BspHI T'CATG_A
Cuts at: 2265 3209 4939 2265
Size: 944 1730 2646
Fragments arranged by size:
2646 1730 944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2435 2675 844
Size: 1591 240 3489
Fragments arranged by size:
3489 1591 240
BsrBI CCG'CTC
Cuts at: 1394 1823 3207 1394
Size: 429 1384 3507
Fragments arranged by size:
3507 1384 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2328 3815 3989 65
Size: 1183 1080 1487 174 1396
Fragments arranged by size:
1487 1396 1183 1080 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2145 3964 4151 1700
Size: 110 335 1819 187 2869
Fragments arranged by size:
2869 1819 335 187 110
BsrGI T'GTAC_A
Cuts at: 96 2151 96
Size: 2055 3265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5320
BssSI C'ACGA_G
Cuts at: 3380 4392 3380
Size: 1012 4308
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5320
BstBI TT'CG_AA
Cuts at: 4124 4124
Size: 5320
BstEII G'GTnAC_C
Cuts at: 1184 2107 2141 1184
Size: 923 34 4363
Fragments arranged by size:
4363 923 34
BstYI r'GATC_y
Cuts at: 1972 2091 2127 2479 2650 3017 3402 3419 4871
Size: 119 36 352 171 367 385 17 1452
Cuts at: 4871 4957 4969 5315 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 352 346 171 119
86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2112 2136 2394 513
Size: 552 1047 24 258 3439
Fragments arranged by size:
3439 1047 552 258 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2053 487
Size: 683 56 827 3754
Fragments arranged by size:
3754 827 683 56
BtsI GCAGTG_nn'
Cuts at: 2547 2836 3630 3650 2547
Size: 289 794 20 4217
Fragments arranged by size:
4217 794 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5285
DraI TTT'AAA
Cuts at: 2624 2624
Size: 5320
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2414 4327 809
Size: 507 1098 1913 1802
Fragments arranged by size:
1913 1802 1098 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2064 2163
Size: 54 1425 50 275 18 210 24 99
Cuts at: 2163 2277 2767 3656 5285 8
Size: 114 490 889 1629 43
Fragments arranged by size:
1629 1425 889 490 275 210 114 99
54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2767 1537
Size: 275 955 4090
Fragments arranged by size:
4090 955 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3250 1353
Size: 1897 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3907 4281 4427 387
Size: 1064 138 2318 374 146 1280
Fragments arranged by size:
2318 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2624 3383 3980 4767 5035 1239
Size: 393 992 759 597 787 268 1524
Fragments arranged by size:
1524 992 787 759 597 393 268
EcoRI G'AATT_C
Cuts at: 2629 3007 2629
Size: 378 4942
FspI TGC'GCA
Cuts at: 3826 3826
Size: 5320
HaeII r_GCGC'y
Cuts at: 1678 2086 2203 4467 1678
Size: 408 117 2264 2531
Fragments arranged by size:
2531 2264 408 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 2528 2547 2560
Size: 32 135 32 447 32 477 19 13
Cuts at: 2560 2579 3961 3993 4035 4067 1373
Size: 19 1382 32 42 32 2626
Fragments arranged by size:
2626 1382 477 447 135 42 32 32
32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2664 2910 669
Size: 988 281 726 246 3079
Fragments arranged by size:
3079 988 726 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5320
HpaI GTT'AAC
Cuts at: 2910 2910
Size: 5320
KpnI G_GTAC'C
Cuts at: 2645 2645
Size: 5320
MfeI C'AATT_G
Cuts at: 2919 2919
Size: 5320
MluI A'CGCG_T
Cuts at: 5196 5196
Size: 5320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2319 4434 4618 1305
Size: 1014 2115 184 2007
Fragments arranged by size:
2115 2007 1014 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 2066 2279 10
Size: 54 1425 343 210 24 213 3051
Fragments arranged by size:
3051 1425 343 213 210 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2270 3278 3637 3796 518
Size: 648 876 228 1008 359 159 2042
Fragments arranged by size:
2042 1008 876 648 359 228 159
MspA1I CmG'CkG
Cuts at: 1606 2736 3413 4561 4806 1606
Size: 1130 677 1148 245 2120
Fragments arranged by size:
2120 1148 1130 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2136 2394 513
Size: 552 1071 258 3439
Fragments arranged by size:
3439 1071 552 258
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5320
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5320
NotI GC'GGCC_GC
Cuts at: 2767 2767
Size: 5320
NspI r_CATG'y
Cuts at: 1132 1229 2678 4223 1132
Size: 97 1449 1545 2229
Fragments arranged by size:
2229 1545 1449 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5320
PciI A'CATG_T
Cuts at: 4219 4219
Size: 5320
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5320
PmeI GTTT'AAAC
Cuts at: 2624 2624
Size: 5320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3389 3421 4902 4934 3389
Size: 32 1481 32 3775
Fragments arranged by size:
3775 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5320
PsiI TTA'TAA
Cuts at: 2890 2890
Size: 5320
PstI C_TGCA'G
Cuts at: 830 2077 2672 830
Size: 1247 595 3478
Fragments arranged by size:
3478 1247 595
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5320
SalI G'TCGA_C
Cuts at: 1936 2662 1936
Size: 726 4594
SbfI CC_TGCA'GG
Cuts at: 2672 2672
Size: 5320
ScaI AGT'ACT
Cuts at: 3568 5280 3568
Size: 1712 3608
SfcI C'TryA_G
Cuts at: 826 1045 2073 2668 3803 4484 4675 826
Size: 219 1028 595 1135 681 191 1471
Fragments arranged by size:
1471 1135 1028 681 595 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2039 2039
Size: 5320
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5320
SmaI CCC'GGG
Cuts at: 1872 2647 1872
Size: 775 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2204 3424 4325 4587 820
Size: 197 926 261 1220 901 262 1553
Fragments arranged by size:
1553 1220 926 901 262 261 197
SnaBI TAC'GTA
Cuts at: 493 3111 493
Size: 2618 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5320
SphI G_CATG'C
Cuts at: 1132 2678 1132
Size: 1546 3774
SspI AAT'ATT
Cuts at: 5 52 3244 5
Size: 47 3192 2081
Fragments arranged by size:
3192 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5320
StyI C'CwwG_G
Cuts at: 513 1065 2136 2394 2759 513
Size: 552 1071 258 365 3074
Fragments arranged by size:
3074 1071 552 365 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 2147 3347 3364 3517 3702 4147
Size: 1255 549 18 1200 17 153 185 445
Cuts at: 4147 325
Size: 1498
Fragments arranged by size:
1498 1255 1200 549 445 185 153 18
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 3566 5278
Size: 274 80 33 51 1443 174 1415 1712
Cuts at: 5278 96
Size: 138
Fragments arranged by size:
1712 1443 1415 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2044 2076 5280 5312 2044
Size: 32 3204 32 2052
Fragments arranged by size:
3204 2052 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5320
XbaI T'CTAG_A
Cuts at: 2374 2656 2374
Size: 282 5038
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5320
XmaI C'CCGG_G
Cuts at: 1870 2645 1870
Size: 775 4545
XmnI GAAnn'nnTTC
Cuts at: 3449 3449
Size: 5320
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2182 3128 276
Size: 53 83 186 936 648 946 2468
Fragments arranged by size:
2468 946 936 648 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI