Restriction map for
pFN21AB6904
(Circular) (Six-base) MAPSORT of: pFN21AB6904.seq Check: 2716 from: 1 to: 5374
pFN21AB6904 5374 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2271 3437 4821 5089 1632
Size: 639 1166 1384 268 1917
Fragments arranged by size:
1917 1384 1166 639 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2132 1184
Size: 948 4426
AlwNI CAG_nnn'CTG
Cuts at: 2061 2483 3117 4689 2061
Size: 422 634 1572 2746
Fragments arranged by size:
2746 1572 634 422
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2683 2909 3061 5170 1493
Size: 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2699 1870
Size: 73 756 4545
Fragments arranged by size:
4545 756 73
BamHI G'GATC_C
Cuts at: 2499 2704 3071 2499
Size: 205 367 4802
Fragments arranged by size:
4802 367 205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2318 2695 4150 618
Size: 325 217 627 531 377 1455 1842
Fragments arranged by size:
1842 1455 627 531 377 325 217
BanII G_rGCy'C
Cuts at: 1391 2403 2693 1391
Size: 1012 290 4072
Fragments arranged by size:
4072 1012 290
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 928
Size: 971 196 4207
Fragments arranged by size:
4207 971 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2499 2533 3565 3599 1903
Size: 34 562 34 1032 34 3678
Fragments arranged by size:
3678 1032 562 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BfrBI ATG'CAT
Cuts at: 2186 2186
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3985 136
Size: 107 122 71 631 605 2313 1525
Fragments arranged by size:
2313 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2037 2791 2037
Size: 754 4620
Bme1580I G_kGCm'C
Cuts at: 1790 2321 2477 3435 4591 1790
Size: 531 156 958 1156 2573
Fragments arranged by size:
2573 1156 958 531 156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3105 4063 442
Size: 416 53 83 399 24 1688 958 1753
Fragments arranged by size:
1753 1688 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2175 2308 4034 1239
Size: 936 133 1726 2579
Fragments arranged by size:
2579 1726 936 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4037 882
Size: 617 2538 2219
Fragments arranged by size:
2538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2122 3165 493
Size: 711 918 1043 2702
Fragments arranged by size:
2702 1043 918 711
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3181 3563 4198 275
Size: 53 83 186 936 1648 382 635 1451
Fragments arranged by size:
1648 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2420 1178
Size: 30 165 30 987 30 4132
Fragments arranged by size:
4132 987 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2483 2534 1186
Size: 1297 51 4026
Fragments arranged by size:
4026 1297 51
BseYI C'CCAG_C
Cuts at: 2127 4577 5073 2127
Size: 2450 496 2428
Fragments arranged by size:
2450 2428 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2053 2296 1163
Size: 573 317 243 4241
Fragments arranged by size:
4241 573 317 243
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3585 4613 1540
Size: 275 186 823 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 3435 3520 4591 2403
Size: 1032 85 1071 3186
Fragments arranged by size:
3186 1071 1032 85
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2321 2403 2477 2693 3435 3520 4591
Size: 399 531 82 74 216 742 85 1071
Cuts at: 4591 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 531 399 216 85 82
74
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 844
Size: 1885 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 1394
Size: 429 1438 3507
Fragments arranged by size:
3507 1438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2121 3869 4043 65
Size: 1183 873 1748 174 1396
Fragments arranged by size:
1748 1396 1183 873 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4018 4205 1700
Size: 110 2208 187 2869
Fragments arranged by size:
2869 2208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 3434 4446 3434
Size: 1012 4362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5374
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2499 2704 3071 3456 3473 4925 5011 5023
Size: 527 205 367 385 17 1452 86 12
Cuts at: 5023 5369 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 527 385 367 346 205 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2367 513
Size: 552 939 363 3520
Fragments arranged by size:
3520 939 552 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2146 487
Size: 683 56 920 3715
Fragments arranged by size:
3715 920 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3684 3704 2890
Size: 794 20 4560
Fragments arranged by size:
4560 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2103 2138 529
Size: 35 1539 35 3765
Fragments arranged by size:
3765 1539 35 35
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2821 3710
Size: 54 1425 50 275 18 315 676 889
Cuts at: 3710 5339 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 676 315 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2821 1537
Size: 275 1009 4090
Fragments arranged by size:
4090 1009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2096 3304 1353
Size: 743 1208 3423
Fragments arranged by size:
3423 1208 743
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2401 2401
Size: 5374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2271 2308 3437 4034 4821 5089
Size: 393 543 96 37 1129 597 787 268
Cuts at: 5089 1239
Size: 1524
Fragments arranged by size:
1524 1129 787 597 543 393 268 96
37
EcoO109I rG'GnC_Cy
Cuts at: 2058 2058
Size: 5374
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 4521 1678
Size: 2843 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2171 2203 2370 2402 4015
Size: 32 135 32 599 32 167 32 1613
Cuts at: 4015 4047 4089 4121 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1613 599 167 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2289 2718 2964 669
Size: 988 281 351 429 246 3079
Fragments arranged by size:
3079 988 429 351 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4488 4672 1305
Size: 3183 184 2007
Fragments arranged by size:
3183 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 10
Size: 54 1425 343 315 3237
Fragments arranged by size:
3237 1425 343 315 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3332 3691 3850 518
Size: 648 2166 359 159 2042
Fragments arranged by size:
2166 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2041 2552 2790 3467 4615 4860 1606
Size: 420 15 511 238 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 511 420 245 238 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 2367 513
Size: 552 939 363 3520
Fragments arranged by size:
3520 939 552 363
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NsiI A_TGCA'T
Cuts at: 2188 2188
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5374
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2061 1108
Size: 953 4421
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PmlI CAC'GTG
Cuts at: 2122 2122
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 2058
Size: 5374
PshAI GACnn'nnGTC
Cuts at: 1613 2252 1613
Size: 639 4735
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2726 830
Size: 1896 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
PvuII CAG'CTG
Cuts at: 2041 2552 2041
Size: 511 4863
SacI G_AGCT'C
Cuts at: 2403 2403
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2722 3857 4538 4729 826
Size: 219 1677 1135 681 191 1471
Fragments arranged by size:
1677 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2701 1872
Size: 829 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4379 4641 820
Size: 197 926 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2004 2014 2353 2367 2813 513
Size: 552 939 10 339 14 446 3074
Fragments arranged by size:
3074 939 552 446 339 14 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 3418 3571 3756 4201 325
Size: 1255 1821 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3620 5332 96
Size: 274 80 33 51 1443 1643 1712 138
Fragments arranged by size:
1712 1643 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2322 2354 5334 5366 2322
Size: 32 2980 32 2330
Fragments arranged by size:
2980 2330 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5374
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2699 1870
Size: 829 4545
XmnI GAAnn'nnTTC
Cuts at: 3503 3503
Size: 5374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI