Restriction map for
pFN21AB6902
(Circular) (Six-base) MAPSORT of: pFN21AB6902.seq Check: 8598 from: 1 to: 5374
pFN21AB6902 5374 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2269 2269
Size: 5374
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2073 3184 278
Size: 53 83 186 936 537 1111 2468
Fragments arranged by size:
2468 1111 936 537 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3437 4821 5089 1632
Size: 1805 1384 268 1917
Fragments arranged by size:
1917 1805 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2558 1184
Size: 1374 4000
AlwNI CAG_nnn'CTG
Cuts at: 2080 2521 3117 4689 2080
Size: 441 596 1572 2765
Fragments arranged by size:
2765 1572 596 441
ApaI G_GGCC'C
Cuts at: 2399 2399
Size: 5374
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2683 2909 3061 5170 1493
Size: 1190 226 152 2109 1697
Fragments arranged by size:
2109 1697 1190 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2699 1870
Size: 73 388 368 4545
Fragments arranged by size:
4545 388 368 73
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2695 4150 618
Size: 325 217 627 264 644 1455 1842
Fragments arranged by size:
1842 1455 644 627 325 264 217
BanII G_rGCy'C
Cuts at: 1391 2399 2693 1391
Size: 1008 294 4072
Fragments arranged by size:
4072 1008 294
BbeI G_GCGC'C
Cuts at: 2055 2055
Size: 5374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4403
BbvCI CC'TCA_GC
Cuts at: 2343 2343
Size: 5374
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3565 3599 1903
Size: 34 1628 34 3678
Fragments arranged by size:
3678 1628 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 2257 1548
Size: 709 4665
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2148 3985 136
Size: 107 122 71 631 605 476 1837 1525
Fragments arranged by size:
1837 1525 631 605 476 122 107 71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 2399 3435 4591 1790
Size: 609 1036 1156 2573
Fragments arranged by size:
2573 1156 1036 609
BmgBI CAC'GTC
Cuts at: 1318 2131 2203 1318
Size: 813 72 4489
Fragments arranged by size:
4489 813 72
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2384 3105 4063
Size: 416 53 83 399 24 967 721 958
Cuts at: 4063 442
Size: 1753
Fragments arranged by size:
1753 967 958 721 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4034 1239
Size: 2795 2579
Bpu10I CC'TnA_GC
Cuts at: 2343 2343
Size: 5374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3499 4364 4662 3499
Size: 865 298 4211
Fragments arranged by size:
4211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4037 882
Size: 617 2538 2219
Fragments arranged by size:
2538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3165 493
Size: 711 1961 2702
Fragments arranged by size:
2702 1961 711
BsaBI GATnn'nnATC
Cuts at: 5368 5368
Size: 5374
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2070 3181 3563
Size: 53 83 186 936 519 18 1111 382
Cuts at: 3563 4198 275
Size: 635 1451
Fragments arranged by size:
1451 1111 936 635 519 382 186 83
53 18
BsaWI w'CCGG_w
Cuts at: 3807 4205 4479 4626 5037 3807
Size: 398 274 147 411 4144
Fragments arranged by size:
4144 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5149
Fragments arranged by size:
5149 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 2111 2300 1186
Size: 843 82 189 4260
Fragments arranged by size:
4260 843 189 82
BseYI C'CCAG_C
Cuts at: 2443 2625 2644 4577 5073 2443
Size: 182 19 1933 496 2744
Fragments arranged by size:
2744 1933 496 182 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 2288 2564 1163
Size: 573 543 9 276 3973
Fragments arranged by size:
3973 573 543 276 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3585 4613 1540
Size: 275 186 823 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3435 3520 4591 3435
Size: 85 1071 4218
Fragments arranged by size:
4218 1071 85
BsmI GAATG_Cn'
Cuts at: 2885 2978 2885
Size: 93 5281
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2399 2693 3435 3520 4591 1391
Size: 399 609 294 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 609 399 294 85
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 3263 4993 3263
Size: 1730 3644
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2269 2729 844
Size: 1425 460 3489
Fragments arranged by size:
3489 1425 460
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 3261 1394
Size: 429 208 1230 3507
Fragments arranged by size:
3507 1230 429 208
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 4018 4205 1700
Size: 110 238 1970 187 2869
Fragments arranged by size:
2869 1970 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5374
BssSI C'ACGA_G
Cuts at: 3434 4446 3434
Size: 1012 4362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2302 794
Size: 1508 3866
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstXI CCAn_nnnn'nTGG
Cuts at: 2625 2625
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369
Size: 732 367 385 17 1452 86 12 346
Cuts at: 5369 1972
Size: 1977
Fragments arranged by size:
1977 1452 732 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2402 513
Size: 552 1337 3485
Fragments arranged by size:
3485 1337 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2437 487
Size: 683 56 1211 3424
Fragments arranged by size:
3424 1211 683 56
BtsI GCAGTG_nn'
Cuts at: 2274 2519 2542 2890 3684 3704 2274
Size: 245 23 348 794 20 3944
Fragments arranged by size:
3944 794 348 245 23 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2821 3710 5339
Size: 54 1425 50 275 18 991 889 1629
Cuts at: 5339 8
Size: 43
Fragments arranged by size:
1629 1425 991 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2821 1537
Size: 275 1009 4090
Fragments arranged by size:
4090 1009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3304 1353
Size: 1951 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3437 4034 4821 5089 1239
Size: 393 1805 597 787 268 1524
Fragments arranged by size:
1805 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2290 2395 2396 2290
Size: 105 1 5268
Fragments arranged by size:
5268 105 1
EcoRI G'AATT_C
Cuts at: 2683 3061 2683
Size: 378 4996
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 2055 4521 1678
Size: 377 2466 2531
Fragments arranged by size:
2531 2466 377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373
Size: 32 135 32 2443 32 42 32 2626
Fragments arranged by size:
2626 2443 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KasI G'GCGC_C
Cuts at: 2051 2051
Size: 5374
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 2642 4488 4672 1305
Size: 729 608 1846 184 2007
Fragments arranged by size:
2007 1846 729 608 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3552
Fragments arranged by size:
3552 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2156 3332 3691 3850 518
Size: 648 990 1176 359 159 2042
Fragments arranged by size:
2042 1176 990 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2302 2790 3467 4615 4860 1606
Size: 564 132 488 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 564 488 245 132
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 5374
NarI GG'CG_CC
Cuts at: 2052 2052
Size: 5374
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4822
NdeI CA'TA_TG
Cuts at: 387 2243 387
Size: 1856 3518
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NruI TCG'CGA
Cuts at: 2313 2313
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5374
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5374
PfoI T'CCnGG_A
Cuts at: 2073 2073
Size: 5374
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PspOMI G'GGCC_C
Cuts at: 2395 2395
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2253 2726 830
Size: 1423 473 3478
Fragments arranged by size:
3478 1423 473
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
PvuII CAG'CTG
Cuts at: 2302 2302
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2249 2722 3857 4538 4729 826
Size: 219 1204 473 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 681 473 219 191
SfoI GGC'GCC
Cuts at: 2053 2053
Size: 5374
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2333 2701 1872
Size: 461 368 4545
Fragments arranged by size:
4545 461 368
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3478 4379 4641 820
Size: 197 926 1535 901 262 1553
Fragments arranged by size:
1553 1535 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2813 513
Size: 552 1748 3074
Fragments arranged by size:
3074 1748 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2519 3401 3418 3571 3756 4201 325
Size: 1255 939 882 17 153 185 445 1498
Fragments arranged by size:
1498 1255 939 882 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3620 5332 96
Size: 274 80 33 51 1443 1643 1712 138
Fragments arranged by size:
1712 1643 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5334 5366 5334
Size: 32 5342
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5374
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2150 2150
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2331 2699 1870
Size: 461 368 4545
Fragments arranged by size:
4545 461 368
XmnI GAAnn'nnTTC
Cuts at: 3503 3503
Size: 5374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2071 3182 276
Size: 53 83 186 936 537 1111 2468
Fragments arranged by size:
2468 1111 936 537 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI
FspAI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI