Restriction map for
pFN21AB6901
(Circular) (Six-base) MAPSORT of: pFN21AB6901.seq Check: 1590 from: 1 to: 5314
pFN21AB6901 5314 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2220 3124 278
Size: 53 83 186 936 684 904 2468
Fragments arranged by size:
2468 936 904 684 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2657 1937
Size: 720 4594
Acc65I G'GTAC_C
Cuts at: 2635 2635
Size: 5314
AclI AA'CG_TT
Cuts at: 1563 2464 3441 3814 1563
Size: 901 977 373 3063
Fragments arranged by size:
3063 977 901 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3377 4761 5029 1632
Size: 1745 1384 268 1917
Fragments arranged by size:
1917 1745 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5117
AflIII A'CryG_T
Cuts at: 1203 4213 5190 1203
Size: 3010 977 1327
Fragments arranged by size:
3010 1327 977
AgeI A'CCGG_T
Cuts at: 4145 4145
Size: 5314
AhdI GACnn_n'nnGTC
Cuts at: 4043 4043
Size: 5314
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5314
AlwNI CAG_nnn'CTG
Cuts at: 3057 4629 3057
Size: 1572 3742
ApaLI G'TGCA_C
Cuts at: 3371 4527 3371
Size: 1156 4158
ApoI r'AATT_y
Cuts at: 1493 2244 2623 2849 3001 5110 1493
Size: 751 379 226 152 2109 1697
Fragments arranged by size:
2109 1697 751 379 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3868 160
Size: 872 2836 1606
Fragments arranged by size:
2836 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
AvaI C'yCGr_G
Cuts at: 1870 1943 2639 1870
Size: 73 696 4545
Fragments arranged by size:
4545 696 73
BamHI G'GATC_C
Cuts at: 2644 3011 2644
Size: 367 4947
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 2635 4090 618
Size: 325 217 627 311 537 1455 1842
Fragments arranged by size:
1842 1455 627 537 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2060 2633 1391
Size: 669 573 4072
Fragments arranged by size:
4072 669 573
BbeI G_GCGC'C
Cuts at: 2102 2102
Size: 5314
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2296 928
Size: 971 397 3946
Fragments arranged by size:
3946 971 397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3505 3539 1903
Size: 34 1568 34 3678
Fragments arranged by size:
3678 1568 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3206 4416 3206
Size: 1210 4104
BclI T'GATC_A
Cuts at: 1548 2566 1548
Size: 1018 4296
BfrBI ATG'CAT
Cuts at: 2354 2354
Size: 5314
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3925 136
Size: 107 122 71 631 605 2253 1525
Fragments arranged by size:
2253 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5309 5309
Size: 5314
BlpI GC'TnA_GC
Cuts at: 2731 2731
Size: 5314
Bme1580I G_kGCm'C
Cuts at: 1790 3375 4531 1790
Size: 1585 1156 2573
Fragments arranged by size:
2573 1585 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3045 4003 442
Size: 416 53 83 399 24 1628 958 1753
Fragments arranged by size:
1753 1628 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2604 1056
Size: 1548 3766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3974 1239
Size: 2735 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3439 4304 4602 3439
Size: 865 298 4151
Fragments arranged by size:
4151 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3977 882
Size: 617 2478 2219
Fragments arranged by size:
2478 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3105 493
Size: 711 1901 2702
Fragments arranged by size:
2702 1901 711
BsaBI GATnn'nnATC
Cuts at: 5308 5308
Size: 5314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 2099 2217 3121
Size: 53 83 186 936 530 36 118 904
Cuts at: 3121 3503 4138 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 904 635 530 382 186 118
83 53 36
BsaWI w'CCGG_w
Cuts at: 3747 4145 4419 4566 4977 3747
Size: 398 274 147 411 4084
Fragments arranged by size:
4084 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2207 2237 1178
Size: 30 165 30 804 30 4255
Fragments arranged by size:
4255 804 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2212 2351 1186
Size: 1026 139 4149
Fragments arranged by size:
4149 1026 139
BseYI C'CCAG_C
Cuts at: 4517 5013 4517
Size: 496 4818
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4741
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 2136 2169 2764 3525 4553
Size: 275 186 111 24 33 595 761 1028
Cuts at: 4553 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 595 275 186 111 33
24
BsiHKAI G_wGCw'C
Cuts at: 3375 3460 4531 3375
Size: 85 1071 4158
Fragments arranged by size:
4158 1071 85
BsmI GAATG_Cn'
Cuts at: 2283 2825 2918 2283
Size: 542 93 4679
Fragments arranged by size:
4679 542 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2224 2224
Size: 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2633 3375 3460 4531 1391
Size: 399 270 573 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 573 399 270 85
BspEI T'CCGG_A
Cuts at: 4977 4977
Size: 5314
BspHI T'CATG_A
Cuts at: 3203 4933 3203
Size: 1730 3584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2434 2669 844
Size: 1590 235 3489
Fragments arranged by size:
3489 1590 235
BsrBI CCG'CTC
Cuts at: 1394 1823 3201 1394
Size: 429 1378 3507
Fragments arranged by size:
3507 1378 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3809 3983 65
Size: 1183 2561 174 1396
Fragments arranged by size:
2561 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3958 4145 1700
Size: 110 2148 187 2869
Fragments arranged by size:
2869 2148 187 110
BsrGI T'GTAC_A
Cuts at: 96 2250 96
Size: 2154 3160
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5314
BssSI C'ACGA_G
Cuts at: 3374 4386 3374
Size: 1012 4302
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5314
BstBI TT'CG_AA
Cuts at: 4118 4118
Size: 5314
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5314
BstYI r'GATC_y
Cuts at: 1972 2320 2644 3011 3396 3413 4865 4951 4963
Size: 348 324 367 385 17 1452 86 12
Cuts at: 4963 5309 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 348 346 324 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2136 513
Size: 552 939 132 3691
Fragments arranged by size:
3691 939 552 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2095 487
Size: 683 56 869 3706
Fragments arranged by size:
3706 869 683 56
BtsI GCAGTG_nn'
Cuts at: 2830 3624 3644 2830
Size: 794 20 4500
Fragments arranged by size:
4500 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5279
DraI TTT'AAA
Cuts at: 2618 2618
Size: 5314
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4321 809
Size: 507 3005 1802
Fragments arranged by size:
3005 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2133 2139
Size: 54 1425 50 275 18 279 24 6
Cuts at: 2139 2166 2761 3650 5279 8
Size: 27 595 889 1629 43
Fragments arranged by size:
1629 1425 889 595 279 275 54 50
43 27 24 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2109 2133 2166 2761 1537
Size: 275 297 24 33 595 4090
Fragments arranged by size:
4090 595 297 275 33 24
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 2478 3244 1353
Size: 926 199 766 3423
Fragments arranged by size:
3423 926 766 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3901 4275 4421 387
Size: 1064 138 2312 374 146 1280
Fragments arranged by size:
2312 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3377 3974 4761 5029 1239
Size: 393 1745 597 787 268 1524
Fragments arranged by size:
1745 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2623 3001 2623
Size: 378 4936
FspI TGC'GCA
Cuts at: 3820 3820
Size: 5314
HaeII r_GCGC'y
Cuts at: 1678 2102 4461 1678
Size: 424 2359 2531
Fragments arranged by size:
2531 2359 424
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2207 2239 3955 3987 4029
Size: 32 135 32 635 32 1716 32 42
Cuts at: 4029 4061 1373
Size: 32 2626
Fragments arranged by size:
2626 1716 635 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2658 2904 669
Size: 988 281 720 246 3079
Fragments arranged by size:
3079 988 720 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5314
HpaI GTT'AAC
Cuts at: 2904 2904
Size: 5314
KasI G'GCGC_C
Cuts at: 2098 2098
Size: 5314
KpnI G_GTAC'C
Cuts at: 2639 2639
Size: 5314
MfeI C'AATT_G
Cuts at: 2913 2913
Size: 5314
MluI A'CGCG_T
Cuts at: 5190 5190
Size: 5314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2335 4428 4612 1305
Size: 1030 2093 184 2007
Fragments arranged by size:
2093 2007 1030 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3492
Fragments arranged by size:
3492 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3272 3631 3790 518
Size: 648 2106 359 159 2042
Fragments arranged by size:
2106 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2114 2138 2162 2730 3407 4555 4800
Size: 444 64 24 24 568 677 1148 245
Cuts at: 4800 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 568 444 245 64 24
24
NarI GG'CG_CC
Cuts at: 2099 2099
Size: 5314
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3823
Fragments arranged by size:
3823 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5314
NheI G'CTAG_C
Cuts at: 1052 2600 1052
Size: 1548 3766
NotI GC'GGCC_GC
Cuts at: 2133 2166 2761 2133
Size: 33 595 4686
Fragments arranged by size:
4686 595 33
NsiI A_TGCA'T
Cuts at: 2356 2356
Size: 5314
NspI r_CATG'y
Cuts at: 1132 1229 2354 2672 4217 1132
Size: 97 1125 318 1545 2229
Fragments arranged by size:
2229 1545 1125 318 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5314
PciI A'CATG_T
Cuts at: 4213 4213
Size: 5314
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5314
PmeI GTTT'AAAC
Cuts at: 2618 2618
Size: 5314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 4896 4928 3383
Size: 32 1481 32 3769
Fragments arranged by size:
3769 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5314
PsiI TTA'TAA
Cuts at: 2884 2884
Size: 5314
PstI C_TGCA'G
Cuts at: 830 2398 2666 830
Size: 1568 268 3478
Fragments arranged by size:
3478 1568 268
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5314
SacII CC_GC'GG
Cuts at: 2139 2139
Size: 5314
SalI G'TCGA_C
Cuts at: 1936 2656 1936
Size: 720 4594
SbfI CC_TGCA'GG
Cuts at: 2666 2666
Size: 5314
ScaI AGT'ACT
Cuts at: 3562 5274 3562
Size: 1712 3602
SfcI C'TryA_G
Cuts at: 826 1045 2394 2662 3797 4478 4669 826
Size: 219 1349 268 1135 681 191 1471
Fragments arranged by size:
1471 1349 1135 681 268 219 191
SfoI GGC'GCC
Cuts at: 2100 2100
Size: 5314
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5314
SmaI CCC'GGG
Cuts at: 1872 2641 1872
Size: 769 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3418 4319 4581 820
Size: 197 926 1475 901 262 1553
Fragments arranged by size:
1553 1475 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3105 493
Size: 2612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5314
SphI G_CATG'C
Cuts at: 1132 2672 1132
Size: 1540 3774
SspI AAT'ATT
Cuts at: 5 52 2514 3238 5
Size: 47 2462 724 2081
Fragments arranged by size:
2462 2081 724 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5314
StyI C'CwwG_G
Cuts at: 513 1065 2004 2753 513
Size: 552 939 749 3074
Fragments arranged by size:
3074 939 749 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3341 3358 3511 3696 4141 325
Size: 1255 1761 17 153 185 445 1498
Fragments arranged by size:
1761 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2250 2330 3560
Size: 274 80 33 51 1443 273 80 1230
Cuts at: 3560 5272 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1230 274 273 138 80 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2443 2475 5274 5306 2443
Size: 32 2799 32 2451
Fragments arranged by size:
2799 2451 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5314
XbaI T'CTAG_A
Cuts at: 2650 2650
Size: 5314
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5314
XmaI C'CCGG_G
Cuts at: 1870 2639 1870
Size: 769 4545
XmnI GAAnn'nnTTC
Cuts at: 2244 3443 2244
Size: 1199 4115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2218 3122 276
Size: 53 83 186 936 684 904 2468
Fragments arranged by size:
2468 936 904 684 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI
SwaI XcmI