Restriction map for
pFN21AB6896
(Circular) (Six-base) MAPSORT of: pFN21AB6896.seq Check: 6223 from: 1 to: 5311
pFN21AB6896 5311 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2045 2045
Size: 5311
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 1937
Size: 717 4594
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2540 3374 4758 5026 1632
Size: 641 267 834 1384 268 1917
Fragments arranged by size:
1917 1384 834 641 268 267
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 2066 2480 4210 5187 1203
Size: 863 414 1730 977 1327
Fragments arranged by size:
1730 1327 977 863 414
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 3054 4626 3054
Size: 1572 3739
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2620 2846 2998 5107 1493
Size: 1127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2636 1870
Size: 73 693 4545
Fragments arranged by size:
4545 693 73
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 4087 618
Size: 325 217 627 845 1455 1842
Fragments arranged by size:
1842 1455 845 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2601 2630 1391
Size: 1210 29 4072
Fragments arranged by size:
4072 1210 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2261 2483 928
Size: 971 362 222 3756
Fragments arranged by size:
3756 971 362 222
BbvCI CC'TCA_GC
Cuts at: 2557 2557
Size: 5311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3502 3536 1903
Size: 34 1565 34 3678
Fragments arranged by size:
3678 1565 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2342 3922 136
Size: 107 122 71 631 605 670 1580 1525
Fragments arranged by size:
1580 1525 670 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2352 5306 2352
Size: 2954 2357
BlpI GC'TnA_GC
Cuts at: 2213 2728 2213
Size: 515 4796
Bme1580I G_kGCm'C
Cuts at: 1790 2347 3372 4528 1790
Size: 557 1025 1156 2573
Fragments arranged by size:
2573 1156 1025 557
BmgBI CAC'GTC
Cuts at: 1318 2069 1318
Size: 751 4560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2121 3042 4000
Size: 416 53 83 399 24 704 921 958
Cuts at: 4000 442
Size: 1753
Fragments arranged by size:
1753 958 921 704 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2300 2323 3971 1239
Size: 824 237 23 1648 2579
Fragments arranged by size:
2579 1648 824 237 23
Bpu10I CC'TnA_GC
Cuts at: 2469 2557 2469
Size: 88 5223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4301 4599 3436
Size: 865 298 4148
Fragments arranged by size:
4148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2402 3974 882
Size: 617 903 1572 2219
Fragments arranged by size:
2219 1572 903 617
BsaAI yAC'GTr
Cuts at: 493 1204 2483 3102 493
Size: 711 1279 619 2702
Fragments arranged by size:
2702 1279 711 619
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3118 3500 4135 275
Size: 53 83 186 936 1585 382 635 1451
Fragments arranged by size:
1585 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3744 4142 4416 4563 4974 3744
Size: 398 274 147 411 4081
Fragments arranged by size:
4081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5086
Fragments arranged by size:
5086 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2315 2375 1186
Size: 1099 30 60 4122
Fragments arranged by size:
4122 1099 60 30
BseYI C'CCAG_C
Cuts at: 2098 2182 4514 5010 2098
Size: 84 2332 496 2399
Fragments arranged by size:
2399 2332 496 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4738
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2761 3522 4550 1540
Size: 275 186 760 761 1028 2301
Fragments arranged by size:
2301 1028 761 760 275 186
BsiHKAI G_wGCw'C
Cuts at: 3372 3457 4528 3372
Size: 85 1071 4155
Fragments arranged by size:
4155 1071 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2347 2601 2630 3372 3457 4528 1391
Size: 399 557 254 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 399 254 85 29
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2045 2214 2442 2666 844
Size: 1201 169 228 224 3489
Fragments arranged by size:
3489 1201 228 224 169
BsrBI CCG'CTC
Cuts at: 1394 1823 3198 1394
Size: 429 1375 3507
Fragments arranged by size:
3507 1375 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3955 4142 1700
Size: 110 2145 187 2869
Fragments arranged by size:
2869 2145 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5311
BssSI C'ACGA_G
Cuts at: 3371 4383 3371
Size: 1012 4299
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2041
Size: 5311
BstYI r'GATC_y
Cuts at: 1972 2076 2313 2352 2641 3008 3393 3410 4862
Size: 104 237 39 289 367 385 17 1452
Cuts at: 4862 4948 4960 5306 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 289 237 104
86 39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4759
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2380 2827 3621 3641 2380
Size: 447 794 20 4050
Fragments arranged by size:
4050 794 447 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2440 2475 529
Size: 35 1876 35 3365
Fragments arranged by size:
3365 1876 35 35
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2758 3647
Size: 54 1425 50 275 18 189 739 889
Cuts at: 3647 5276 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 739 275 189 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2758 1537
Size: 275 946 4090
Fragments arranged by size:
4090 946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3241 1353
Size: 1888 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3898 4272 4418 387
Size: 1064 138 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2273 2300 2323 2540 3374 3971
Size: 393 431 210 27 23 217 834 597
Cuts at: 3971 4758 5026 1239
Size: 787 268 1524
Fragments arranged by size:
1524 834 787 597 431 393 268 217
210 27 23
EcoO109I rG'GnC_Cy
Cuts at: 2380 2539 2380
Size: 159 5152
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 4458 1678
Size: 2780 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2585 2617 3952 3984 4026
Size: 32 135 32 1013 32 1335 32 42
Cuts at: 4026 4058 1373
Size: 32 2626
Fragments arranged by size:
2626 1335 1013 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2395 2655 2901 669
Size: 988 281 457 260 246 3079
Fragments arranged by size:
3079 988 457 281 260 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4425 4609 1305
Size: 3120 184 2007
Fragments arranged by size:
3120 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3489
Fragments arranged by size:
3489 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3269 3628 3787 518
Size: 648 2103 359 159 2042
Fragments arranged by size:
2103 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2583 2727 3404 4552 4797 1606
Size: 977 144 677 1148 245 2120
Fragments arranged by size:
2120 1148 977 677 245 144
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4759
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2758 2758
Size: 5311
NspI r_CATG'y
Cuts at: 1132 1229 2457 2669 4214 1132
Size: 97 1228 212 1545 2229
Fragments arranged by size:
2229 1545 1228 212 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5311
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5311
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2539 2539
Size: 5311
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2439 2588 2663 830
Size: 1609 149 75 3478
Fragments arranged by size:
3478 1609 149 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
PvuII CAG'CTG
Cuts at: 2583 2583
Size: 5311
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 2119 3559 5271 2119
Size: 1440 1712 2159
Fragments arranged by size:
2159 1712 1440
SfcI C'TryA_G
Cuts at: 826 1045 2435 2584 2659 3794 4475 4666 826
Size: 219 1390 149 75 1135 681 191 1471
Fragments arranged by size:
1471 1390 1135 681 219 191 149 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2638 1872
Size: 766 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3415 4316 4578 820
Size: 197 926 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5311
StyI C'CwwG_G
Cuts at: 513 1065 2750 513
Size: 552 1685 3074
Fragments arranged by size:
3074 1685 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3338 3355 3508 3693 4138 325
Size: 1255 1758 17 153 185 445 1498
Fragments arranged by size:
1758 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2117 3557 5269
Size: 274 80 33 51 1443 140 1440 1712
Cuts at: 5269 96
Size: 138
Fragments arranged by size:
1712 1443 1440 274 140 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2038 2587 2038
Size: 549 4762
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2636 1870
Size: 766 4545
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI