Restriction map for
pFN21AB6894
(Circular) (Six-base) MAPSORT of: pFN21AB6894.seq Check: 2829 from: 1 to: 5311
pFN21AB6894 5311 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 1937
Size: 717 4594
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3374 4758 5026 1632
Size: 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 4210 5187 1203
Size: 3007 977 1327
Fragments arranged by size:
3007 1327 977
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 3054 4626 3054
Size: 1572 3739
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2620 2846 2998 5107 1493
Size: 1127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2364 2521 2636 1870
Size: 73 421 157 115 4545
Fragments arranged by size:
4545 421 157 115 73
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 2632 4087 618
Size: 325 217 627 555 290 1455 1842
Fragments arranged by size:
1842 1455 627 555 325 290 217
BanII G_rGCy'C
Cuts at: 1391 2075 2630 1391
Size: 684 555 4072
Fragments arranged by size:
4072 684 555
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2527 928
Size: 971 628 3712
Fragments arranged by size:
3712 971 628
BbvCI CC'TCA_GC
Cuts at: 2396 2396
Size: 5311
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3502 3536 1903
Size: 34 1565 34 3678
Fragments arranged by size:
3678 1565 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2597 3922 136
Size: 107 122 71 631 605 925 1325 1525
Fragments arranged by size:
1525 1325 925 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5306 5306
Size: 5311
BlpI GC'TnA_GC
Cuts at: 2728 2728
Size: 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2034 2395 3372 4528 1790
Size: 244 361 977 1156 2573
Fragments arranged by size:
2573 1156 977 361 244
BmgBI CAC'GTC
Cuts at: 1318 2500 1318
Size: 1182 4129
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2382 3042 4000
Size: 416 53 83 399 24 965 660 958
Cuts at: 4000 442
Size: 1753
Fragments arranged by size:
1753 965 958 660 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3971 1239
Size: 2732 2579
Bpu10I CC'TnA_GC
Cuts at: 2396 2447 2396
Size: 51 5260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4301 4599 3436
Size: 865 298 4148
Fragments arranged by size:
4148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3974 882
Size: 617 2475 2219
Fragments arranged by size:
2475 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2485 3102 493
Size: 711 1281 617 2702
Fragments arranged by size:
2702 1281 711 617
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3118 3500 4135 275
Size: 53 83 186 936 1585 382 635 1451
Fragments arranged by size:
1585 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3744 4142 4416 4563 4974 3744
Size: 398 274 147 411 4081
Fragments arranged by size:
4081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5086
Fragments arranged by size:
5086 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5311
BseYI C'CCAG_C
Cuts at: 2551 4514 5010 2551
Size: 1963 496 2852
Fragments arranged by size:
2852 1963 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 2491 1163
Size: 573 297 458 3983
Fragments arranged by size:
3983 573 458 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2761 3522 4550 1540
Size: 275 186 760 761 1028 2301
Fragments arranged by size:
2301 1028 761 760 275 186
BsiHKAI G_wGCw'C
Cuts at: 3372 3457 4528 3372
Size: 85 1071 4155
Fragments arranged by size:
4155 1071 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2075 2395 2630 3372 3457 4528
Size: 399 244 41 320 235 742 85 1071
Cuts at: 4528 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 320 244 235 85
41
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 2136 3200 4930 2136
Size: 1064 1730 2517
Fragments arranged by size:
2517 1730 1064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2666 844
Size: 1822 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3198 1394
Size: 429 1375 3507
Fragments arranged by size:
3507 1375 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3955 4142 1700
Size: 110 2145 187 2869
Fragments arranged by size:
2869 2145 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5311
BssSI C'ACGA_G
Cuts at: 3371 4383 3371
Size: 1012 4299
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstXI CCAn_nnnn'nTGG
Cuts at: 2236 2311 2236
Size: 75 5236
BstYI r'GATC_y
Cuts at: 1972 2376 2641 3008 3393 3410 4862 4948 4960
Size: 404 265 367 385 17 1452 86 12
Cuts at: 4960 5306 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 404 385 367 346 265 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4759
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2572 487
Size: 683 56 1346 3226
Fragments arranged by size:
3226 1346 683 56
BtsI GCAGTG_nn'
Cuts at: 2182 2827 3621 3641 2182
Size: 645 794 20 3852
Fragments arranged by size:
3852 794 645 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2230 2265 529
Size: 35 1666 35 3575
Fragments arranged by size:
3575 1666 35 35
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2758 3647 5276
Size: 54 1425 50 275 18 928 889 1629
Cuts at: 5276 8
Size: 43
Fragments arranged by size:
1629 1425 928 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2758 1537
Size: 275 946 4090
Fragments arranged by size:
4090 946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3241 1353
Size: 1888 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3898 4272 4418 387
Size: 1064 138 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3374 3971 4758 5026 1239
Size: 393 1742 597 787 268 1524
Fragments arranged by size:
1742 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 5311
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 4458 1678
Size: 2780 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373
Size: 32 135 32 2380 32 42 32 2626
Fragments arranged by size:
2626 2380 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2095 2561 2655 2901 669
Size: 988 281 157 466 94 246 3079
Fragments arranged by size:
3079 988 466 281 246 157 94
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4425 4609 1305
Size: 3120 184 2007
Fragments arranged by size:
3120 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3489
Fragments arranged by size:
3489 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2330 2591 3269 3628 3787 518
Size: 648 1164 261 678 359 159 2042
Fragments arranged by size:
2042 1164 678 648 359 261 159
MspA1I CmG'CkG
Cuts at: 1606 2727 3404 4552 4797 1606
Size: 1121 677 1148 245 2120
Fragments arranged by size:
2120 1148 1121 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4759
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2758 2758
Size: 5311
NspI r_CATG'y
Cuts at: 1132 1229 2669 4214 1132
Size: 97 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 97
PasI CC'CwG_GG
Cuts at: 1348 2212 2350 1348
Size: 864 138 4309
Fragments arranged by size:
4309 864 138
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5311
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PmlI CAC'GTG
Cuts at: 2485 2485
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2663 830
Size: 1833 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2659 3794 4475 4666 826
Size: 219 1614 1135 681 191 1471
Fragments arranged by size:
1614 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2366 2638 1872
Size: 494 272 4545
Fragments arranged by size:
4545 494 272
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3415 4316 4578 820
Size: 197 926 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 2442 2606 1517
Size: 925 164 4222
Fragments arranged by size:
4222 925 164
StyI C'CwwG_G
Cuts at: 513 1065 2338 2750 513
Size: 552 1273 412 3074
Fragments arranged by size:
3074 1273 552 412
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2133 3338 3355 3508 3693 4138 325
Size: 1255 553 1205 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1205 553 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2364 2636 1870
Size: 494 272 4545
Fragments arranged by size:
4545 494 272
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI