Restriction map for
pFN21AB6892
(Circular) (Six-base) MAPSORT of: pFN21AB6892.seq Check: 3242 from: 1 to: 5311
pFN21AB6892 5311 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2060 2060
Size: 5311
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 1937
Size: 717 4594
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3374 4758 5026 1632
Size: 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 268
AfeI AGC'GCT
Cuts at: 2270 2270
Size: 5311
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 4210 5187 1203
Size: 3007 977 1327
Fragments arranged by size:
3007 1327 977
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 2548 2558 3054 4626 2548
Size: 10 496 1572 3233
Fragments arranged by size:
3233 1572 496 10
ApaI G_GGCC'C
Cuts at: 2486 2486
Size: 5311
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2620 2846 2998 5107 1493
Size: 1127 226 152 2109 1697
Fragments arranged by size:
2109 1697 1127 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2636 1870
Size: 73 693 4545
Fragments arranged by size:
4545 693 73
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 4087 618
Size: 325 217 627 845 1455 1842
Fragments arranged by size:
1842 1455 845 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2451 2486 2630 1391
Size: 1060 35 144 4072
Fragments arranged by size:
4072 1060 144 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2086 928
Size: 971 187 4153
Fragments arranged by size:
4153 971 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2479 2513 3502 3536 1903
Size: 34 542 34 989 34 3678
Fragments arranged by size:
3678 989 542 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2053 3203 4413 2053
Size: 1150 1210 2951
Fragments arranged by size:
2951 1210 1150
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5311
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2333 3922 136
Size: 107 122 71 631 605 661 1589 1525
Fragments arranged by size:
1589 1525 661 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5306 5306
Size: 5311
BlpI GC'TnA_GC
Cuts at: 2728 2728
Size: 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2486 3372 4528 1790
Size: 696 886 1156 2573
Fragments arranged by size:
2573 1156 886 696
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5311
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2064 2170 3042
Size: 416 53 83 399 24 647 106 872
Cuts at: 3042 4000 442
Size: 958 1753
Fragments arranged by size:
1753 958 872 647 416 399 106 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 3971 1239
Size: 950 1782 2579
Fragments arranged by size:
2579 1782 950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4301 4599 3436
Size: 865 298 4148
Fragments arranged by size:
4148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3974 882
Size: 617 2475 2219
Fragments arranged by size:
2475 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 493
Size: 711 1898 2702
Fragments arranged by size:
2702 1898 711
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2492 3118 3500 4135
Size: 53 83 186 936 959 626 382 635
Cuts at: 4135 275
Size: 1451
Fragments arranged by size:
1451 959 936 635 626 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3744 4142 4416 4563 4974 3744
Size: 398 274 147 411 4081
Fragments arranged by size:
4081 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5086
Fragments arranged by size:
5086 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 1186
Size: 904 4407
BseYI C'CCAG_C
Cuts at: 4514 5010 4514
Size: 496 4815
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4738
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2761 3522 4550 1540
Size: 275 186 760 761 1028 2301
Fragments arranged by size:
2301 1028 761 760 275 186
BsiHKAI G_wGCw'C
Cuts at: 3372 3457 4528 3372
Size: 85 1071 4155
Fragments arranged by size:
4155 1071 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 2486 2630 3372 3457 4528 1391
Size: 399 661 35 144 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 661 399 144 85 35
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2060 2666 844
Size: 1216 606 3489
Fragments arranged by size:
3489 1216 606
BsrBI CCG'CTC
Cuts at: 1394 1823 3198 1394
Size: 429 1375 3507
Fragments arranged by size:
3507 1375 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2375 3955 4142 1700
Size: 110 565 1580 187 2869
Fragments arranged by size:
2869 1580 565 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5311
BssSI C'ACGA_G
Cuts at: 3371 4383 3371
Size: 1012 4299
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5311
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2056
Size: 5311
BstYI r'GATC_y
Cuts at: 1972 2641 3008 3393 3410 4862 4948 4960 5306
Size: 669 367 385 17 1452 86 12 346
Cuts at: 5306 1972
Size: 1977
Fragments arranged by size:
1977 1452 669 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2597 2597
Size: 5311
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4759
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2827 3621 3641 2827
Size: 794 20 4497
Fragments arranged by size:
4497 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DraIII CAC_nnn'GTG
Cuts at: 2072 2072
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2090 4318 809
Size: 507 774 2228 1802
Fragments arranged by size:
2228 1802 774 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2325 2373 2758
Size: 54 1425 50 275 18 495 48 385
Cuts at: 2758 3647 5276 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 495 385 275 54 50
48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2758 1537
Size: 275 946 4090
Fragments arranged by size:
4090 946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2205 2223 3241 1353
Size: 852 18 1018 3423
Fragments arranged by size:
3423 1018 852 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3898 4272 4418 387
Size: 1064 138 2309 374 146 1280
Fragments arranged by size:
2309 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 3374 3971 4758 5026 1239
Size: 393 557 1185 597 787 268 1524
Fragments arranged by size:
1524 1185 787 597 557 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2102 2164 2102
Size: 62 5249
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FseI GG_CCGG'CC
Cuts at: 2379 2379
Size: 5311
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 2272 2549 4458 1678
Size: 594 277 1909 2531
Fragments arranged by size:
2531 1909 594 277
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373
Size: 32 135 32 2380 32 42 32 2626
Fragments arranged by size:
2626 2380 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2655 2901 669
Size: 988 281 717 246 3079
Fragments arranged by size:
3079 988 717 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4425 4609 1305
Size: 3120 184 2007
Fragments arranged by size:
3120 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 10
Size: 54 1425 343 495 2994
Fragments arranged by size:
2994 1425 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3269 3628 3787 518
Size: 648 2103 359 159 2042
Fragments arranged by size:
2103 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2727 3404 4552 4797 1606
Size: 1121 677 1148 245 2120
Fragments arranged by size:
2120 1148 1121 677 245
NaeI GCC'GGC
Cuts at: 2377 2377
Size: 5311
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4759
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NgoMIV G'CCGG_C
Cuts at: 2375 2375
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2758 2758
Size: 5311
NspI r_CATG'y
Cuts at: 1132 1229 2669 4214 1132
Size: 97 1440 1545 2229
Fragments arranged by size:
2229 1545 1440 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5311
PciI A'CATG_T
Cuts at: 4210 4210
Size: 5311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2160 1108
Size: 1052 4259
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2102 2164 2102
Size: 62 5249
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5311
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PspOMI G'GGCC_C
Cuts at: 2482 2482
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2663 830
Size: 1833 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SanDI GG'GwC_CC
Cuts at: 2102 2102
Size: 5311
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2659 3794 4475 4666 826
Size: 219 1614 1135 681 191 1471
Fragments arranged by size:
1614 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2638 1872
Size: 766 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3415 4316 4578 820
Size: 197 926 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 2526 2605 1517
Size: 1009 79 4223
Fragments arranged by size:
4223 1009 79
StyI C'CwwG_G
Cuts at: 513 1065 2750 513
Size: 552 1685 3074
Fragments arranged by size:
3074 1685 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2083 3338 3355 3508 3693 4138 325
Size: 1255 503 1255 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1255 503 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2186 2201 2218 2233 5271 5303 2186
Size: 15 17 15 3038 32 2194
Fragments arranged by size:
3038 2194 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 2335
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2636 1870
Size: 766 4545
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI