Restriction map for
pFN21AB6890
(Circular) (Six-base) MAPSORT of: pFN21AB6890.seq Check: 5965 from: 1 to: 5311
pFN21AB6890 5311 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2306 2306
Size: 5311
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3121 278
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2654 1937
Size: 717 4594
Acc65I G'GTAC_C
Cuts at: 2632 2632
Size: 5311
AclI AA'CG_TT
Cuts at: 1563 3438 3811 1563
Size: 1875 373 3063
Fragments arranged by size:
3063 1875 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3374 4758 5026 1632
Size: 1742 1384 268 1917
Fragments arranged by size:
1917 1742 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5114
AflIII A'CryG_T
Cuts at: 1203 2214 4210 5187 1203
Size: 1011 1996 977 1327
Fragments arranged by size:
1996 1327 1011 977
AgeI A'CCGG_T
Cuts at: 4142 4142
Size: 5311
AhdI GACnn_n'nnGTC
Cuts at: 4040 4040
Size: 5311
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5311
AlwNI CAG_nnn'CTG
Cuts at: 2264 3054 4626 2264
Size: 790 1572 2949
Fragments arranged by size:
2949 1572 790
ApaI G_GGCC'C
Cuts at: 2195 2195
Size: 5311
ApaLI G'TGCA_C
Cuts at: 3368 4524 3368
Size: 1156 4155
ApoI r'AATT_y
Cuts at: 1493 2159 2620 2846 2998 5107 1493
Size: 666 461 226 152 2109 1697
Fragments arranged by size:
2109 1697 666 461 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3865 160
Size: 872 2833 1606
Fragments arranged by size:
2833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2436 2636 1870
Size: 73 104 389 200 4545
Fragments arranged by size:
4545 389 200 104 73
BamHI G'GATC_C
Cuts at: 2641 3008 2641
Size: 367 4944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2632 4087 618
Size: 325 217 627 845 1455 1842
Fragments arranged by size:
1842 1455 845 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2195 2562 2630 1391
Size: 663 141 367 68 4072
Fragments arranged by size:
4072 663 367 141 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3502 3536 1903
Size: 34 1565 34 3678
Fragments arranged by size:
3678 1565 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3203 4413 3203
Size: 1210 4101
BclI T'GATC_A
Cuts at: 1548 2164 1548
Size: 616 4695
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3922 136
Size: 107 122 71 631 605 2250 1525
Fragments arranged by size:
2250 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5306 5306
Size: 5311
BlpI GC'TnA_GC
Cuts at: 2728 2728
Size: 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2195 3372 4528 1790
Size: 405 1177 1156 2573
Fragments arranged by size:
2573 1177 1156 405
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5311
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2470 3042 4000
Size: 416 53 83 399 24 1053 572 958
Cuts at: 4000 442
Size: 1753
Fragments arranged by size:
1753 1053 958 572 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2138 2393 2504 3971 1239
Size: 821 78 255 111 1467 2579
Fragments arranged by size:
2579 1467 821 255 111 78
Bpu10I CC'TnA_GC
Cuts at: 2364 2364
Size: 5311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 4301 4599 3436
Size: 865 298 4148
Fragments arranged by size:
4148 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2489 3974 882
Size: 617 990 1485 2219
Fragments arranged by size:
2219 1485 990 617
BsaAI yAC'GTr
Cuts at: 493 1204 3102 493
Size: 711 1898 2702
Fragments arranged by size:
2702 1898 711
BsaBI GATnn'nnATC
Cuts at: 5305 5305
Size: 5311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 3118 3500 4135
Size: 53 83 186 936 702 883 382 635
Cuts at: 4135 275
Size: 1451
Fragments arranged by size:
1451 936 883 702 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2070 3744 4142 4416 4563 4974 2070
Size: 1674 398 274 147 411 2407
Fragments arranged by size:
2407 1674 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5086
Fragments arranged by size:
5086 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2573 1186
Size: 1387 3924
BseYI C'CCAG_C
Cuts at: 2403 4514 5010 2403
Size: 2111 496 2704
Fragments arranged by size:
2704 2111 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2113 2284 2521 1163
Size: 573 377 171 237 3953
Fragments arranged by size:
3953 573 377 237 171
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2761 3522 4550 1540
Size: 275 186 760 761 1028 2301
Fragments arranged by size:
2301 1028 761 760 275 186
BsiHKAI G_wGCw'C
Cuts at: 3372 3457 4528 3372
Size: 85 1071 4155
Fragments arranged by size:
4155 1071 85
BsmI GAATG_Cn'
Cuts at: 2822 2915 2822
Size: 93 5218
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2195 2562 2630 3372 3457 4528
Size: 399 264 141 367 68 742 85 1071
Cuts at: 4528 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 367 264 141 85
68
BspEI T'CCGG_A
Cuts at: 4974 4974
Size: 5311
BspHI T'CATG_A
Cuts at: 3200 4930 3200
Size: 1730 3581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2306 2666 844
Size: 1462 360 3489
Fragments arranged by size:
3489 1462 360
BsrBI CCG'CTC
Cuts at: 1394 1823 3198 1394
Size: 429 1375 3507
Fragments arranged by size:
3507 1375 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3806 3980 65
Size: 1183 2558 174 1396
Fragments arranged by size:
2558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3955 4142 1700
Size: 110 2145 187 2869
Fragments arranged by size:
2869 2145 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5311
BssSI C'ACGA_G
Cuts at: 3371 4383 3371
Size: 1012 4299
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5311
BstBI TT'CG_AA
Cuts at: 4115 4115
Size: 5311
BstEII G'GTnAC_C
Cuts at: 1184 2440 1184
Size: 1256 4055
BstYI r'GATC_y
Cuts at: 1972 2169 2641 3008 3393 3410 4862 4948 4960
Size: 197 472 367 385 17 1452 86 12
Cuts at: 4960 5306 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 472 385 367 346 197 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2271 2308 2598 513
Size: 552 939 267 37 290 3226
Fragments arranged by size:
3226 939 552 290 267 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4572
Fragments arranged by size:
4572 683 56
BtsI GCAGTG_nn'
Cuts at: 2101 2827 3621 3641 2101
Size: 726 794 20 3771
Fragments arranged by size:
3771 794 726 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5276
DraI TTT'AAA
Cuts at: 2615 2615
Size: 5311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4318 809
Size: 507 3002 1802
Fragments arranged by size:
3002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2758 3647
Size: 54 1425 50 275 18 177 751 889
Cuts at: 3647 5276 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 751 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2758 1537
Size: 275 946 4090
Fragments arranged by size:
4090 946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3241 1353
Size: 1888 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2123 3898 4272 4418 387
Size: 1064 138 534 1775 374 146 1280
Fragments arranged by size:
1775 1280 1064 534 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2138 2393 2504 3374 3971 4758
Size: 393 428 78 255 111 870 597 787
Cuts at: 4758 5026 1239
Size: 268 1524
Fragments arranged by size:
1524 870 787 597 428 393 268 255
111 78
EcoO109I rG'GnC_Cy
Cuts at: 2192 2503 2192
Size: 311 5000
EcoRI G'AATT_C
Cuts at: 2620 2998 2620
Size: 378 4933
FspI TGC'GCA
Cuts at: 3817 3817
Size: 5311
HaeII r_GCGC'y
Cuts at: 1678 4458 1678
Size: 2780 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373
Size: 32 135 32 2380 32 42 32 2626
Fragments arranged by size:
2626 2380 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2344 2655 2901 669
Size: 988 281 406 311 246 3079
Fragments arranged by size:
3079 988 406 311 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5311
HpaI GTT'AAC
Cuts at: 2901 2901
Size: 5311
KpnI G_GTAC'C
Cuts at: 2636 2636
Size: 5311
MfeI C'AATT_G
Cuts at: 2910 2910
Size: 5311
MluI A'CGCG_T
Cuts at: 5187 5187
Size: 5311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4425 4609 1305
Size: 3120 184 2007
Fragments arranged by size:
3120 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3312
Fragments arranged by size:
3312 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 3269 3628 3787 518
Size: 648 1161 942 359 159 2042
Fragments arranged by size:
2042 1161 942 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2413 2727 3404 4552 4797 1606
Size: 807 314 677 1148 245 2120
Fragments arranged by size:
2120 1148 807 677 314 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2308 2598 513
Size: 552 939 304 290 3226
Fragments arranged by size:
3226 939 552 304 290
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5311
NotI GC'GGCC_GC
Cuts at: 2758 2758
Size: 5311
NspI r_CATG'y
Cuts at: 1132 1229 2218 2669 4214 1132
Size: 97 989 451 1545 2229
Fragments arranged by size:
2229 1545 989 451 97
PasI CC'CwG_GG
Cuts at: 1348 2512 1348
Size: 1164 4147
PciI A'CATG_T
Cuts at: 2214 4210 2214
Size: 1996 3315
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5311
PfoI T'CCnGG_A
Cuts at: 2037 2037
Size: 5311
PmeI GTTT'AAAC
Cuts at: 2615 2615
Size: 5311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 4893 4925 3380
Size: 32 1481 32 3766
Fragments arranged by size:
3766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2503 2503
Size: 5311
PshAI GACnn'nnGTC
Cuts at: 1613 2285 1613
Size: 672 4639
PsiI TTA'TAA
Cuts at: 2881 2881
Size: 5311
PspOMI G'GGCC_C
Cuts at: 2191 2191
Size: 5311
PstI C_TGCA'G
Cuts at: 830 2663 830
Size: 1833 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5311
SalI G'TCGA_C
Cuts at: 1936 2653 1936
Size: 717 4594
SbfI CC_TGCA'GG
Cuts at: 2663 2663
Size: 5311
ScaI AGT'ACT
Cuts at: 3559 5271 3559
Size: 1712 3599
SfcI C'TryA_G
Cuts at: 826 1045 2659 3794 4475 4666 826
Size: 219 1614 1135 681 191 1471
Fragments arranged by size:
1614 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5311
SmaI CCC'GGG
Cuts at: 1872 2438 2638 1872
Size: 566 200 4545
Fragments arranged by size:
4545 566 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3415 4316 4578 820
Size: 197 926 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3102 493
Size: 2609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5311
SphI G_CATG'C
Cuts at: 1132 2669 1132
Size: 1537 3774
SspI AAT'ATT
Cuts at: 5 52 3235 5
Size: 47 3183 2081
Fragments arranged by size:
3183 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5311
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2308 2598 2750 513
Size: 552 939 6 298 290 152 3074
Fragments arranged by size:
3074 939 552 298 290 152 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2264 3338 3355 3508 3693 4138 325
Size: 1255 684 1074 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1074 684 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3557 5269 96
Size: 274 80 33 51 1443 1580 1712 138
Fragments arranged by size:
1712 1580 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5271 5303 5271
Size: 32 5279
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5311
XbaI T'CTAG_A
Cuts at: 2647 2647
Size: 5311
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5311
XmaI C'CCGG_G
Cuts at: 1870 2436 2636 1870
Size: 566 200 4545
Fragments arranged by size:
4545 566 200
XmnI GAAnn'nnTTC
Cuts at: 3440 3440
Size: 5311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3119 276
Size: 53 83 186 936 1585 2468
Fragments arranged by size:
2468 1585 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI