Restriction map for
pFN21AB6879
(Circular) (Six-base) MAPSORT of: pFN21AB6879.seq Check: 3598 from: 1 to: 5245
pFN21AB6879 5245 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3055 278
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2588 1937
Size: 651 4594
Acc65I G'GTAC_C
Cuts at: 2566 2566
Size: 5245
AclI AA'CG_TT
Cuts at: 1563 3372 3745 1563
Size: 1809 373 3063
Fragments arranged by size:
3063 1809 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3308 4692 4960 1632
Size: 1676 1384 268 1917
Fragments arranged by size:
1917 1676 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5048
AflIII A'CryG_T
Cuts at: 1203 4144 5121 1203
Size: 2941 977 1327
Fragments arranged by size:
2941 1327 977
AgeI A'CCGG_T
Cuts at: 4076 4076
Size: 5245
AhdI GACnn_n'nnGTC
Cuts at: 3974 3974
Size: 5245
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5245
AlwNI CAG_nnn'CTG
Cuts at: 2167 2988 4560 2167
Size: 821 1572 2852
Fragments arranged by size:
2852 1572 821
ApaI G_GGCC'C
Cuts at: 2036 2036
Size: 5245
ApaLI G'TGCA_C
Cuts at: 3302 4458 3302
Size: 1156 4089
ApoI r'AATT_y
Cuts at: 1493 2554 2780 2932 5041 1493
Size: 1061 226 152 2109 1697
Fragments arranged by size:
2109 1697 1061 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3799 160
Size: 872 2767 1606
Fragments arranged by size:
2767 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 2570 1870
Size: 73 163 464 4545
Fragments arranged by size:
4545 464 163 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2436 2469 2436
Size: 33 5212
BamHI G'GATC_C
Cuts at: 2269 2575 2942 2269
Size: 306 367 4572
Fragments arranged by size:
4572 367 306
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2566 4021 618
Size: 325 217 627 779 1455 1842
Fragments arranged by size:
1842 1455 779 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 2564 1391
Size: 645 528 4072
Fragments arranged by size:
4072 645 528
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2147 2407 928
Size: 971 248 260 3766
Fragments arranged by size:
3766 971 260 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3436 3470 1903
Size: 34 1499 34 3678
Fragments arranged by size:
3678 1499 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3137 4347 3137
Size: 1210 4035
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5245
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2185 2301 3856
Size: 107 122 71 631 605 513 116 1555
Cuts at: 3856 136
Size: 1525
Fragments arranged by size:
1555 1525 631 605 513 122 116 107
71
BglII A'GATC_T
Cuts at: 5240 5240
Size: 5245
BlpI GC'TnA_GC
Cuts at: 2662 2662
Size: 5245
Bme1580I G_kGCm'C
Cuts at: 1790 2036 3306 4462 1790
Size: 246 1270 1156 2573
Fragments arranged by size:
2573 1270 1156 246
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2976 3934 442
Size: 416 53 83 399 24 1559 958 1753
Fragments arranged by size:
1753 1559 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2331 2331
Size: 5245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2464 3905 1239
Size: 950 275 1441 2579
Fragments arranged by size:
2579 1441 950 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3370 4235 4533 3370
Size: 865 298 4082
Fragments arranged by size:
4082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2302 3908 882
Size: 617 803 1606 2219
Fragments arranged by size:
2219 1606 803 617
BsaAI yAC'GTr
Cuts at: 493 1204 3036 493
Size: 711 1832 2702
Fragments arranged by size:
2702 1832 711
BsaBI GATnn'nnATC
Cuts at: 5239 5239
Size: 5245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3052 3434 4069 275
Size: 53 83 186 936 1519 382 635 1451
Fragments arranged by size:
1519 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2317 3678 4076 4350 4497 4908 2317
Size: 1361 398 274 147 411 2654
Fragments arranged by size:
2654 1361 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 1178
Size: 30 165 30 1059 30 3931
Fragments arranged by size:
3931 1059 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2036 1186
Size: 847 3 4395
Fragments arranged by size:
4395 847 3
BseYI C'CCAG_C
Cuts at: 2210 2473 4448 4944 2210
Size: 263 1975 496 2511
Fragments arranged by size:
2511 1975 496 263
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4672
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2695 3456 4484 1540
Size: 275 186 694 761 1028 2301
Fragments arranged by size:
2301 1028 761 694 275 186
BsiHKAI G_wGCw'C
Cuts at: 3306 3391 4462 3306
Size: 85 1071 4089
Fragments arranged by size:
4089 1071 85
BsmI GAATG_Cn'
Cuts at: 2756 2849 2756
Size: 93 5152
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2564 3306 3391 4462 1391
Size: 399 246 528 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 528 399 246 85
BspEI T'CCGG_A
Cuts at: 2317 4908 2317
Size: 2591 2654
BspHI T'CATG_A
Cuts at: 3134 4864 3134
Size: 1730 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2600 844
Size: 1756 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2056 3132 1394
Size: 429 233 1076 3507
Fragments arranged by size:
3507 1076 429 233
BsrDI GCAATG_nn'
Cuts at: 65 1248 3740 3914 65
Size: 1183 2492 174 1396
Fragments arranged by size:
2492 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3889 4076 1700
Size: 110 2079 187 2869
Fragments arranged by size:
2869 2079 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5245
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5245
BssSI C'ACGA_G
Cuts at: 3305 4317 3305
Size: 1012 4233
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5245
BstBI TT'CG_AA
Cuts at: 2079 4049 2079
Size: 1970 3275
BstEII G'GTnAC_C
Cuts at: 1184 2468 1184
Size: 1284 3961
BstXI CCAn_nnnn'nTGG
Cuts at: 2345 2345
Size: 5245
BstYI r'GATC_y
Cuts at: 1972 2269 2455 2575 2942 3327 3344 4796 4882
Size: 297 186 120 367 385 17 1452 86
Cuts at: 4882 4894 5240 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 297 186 120
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3754
Fragments arranged by size:
3754 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4506
Fragments arranged by size:
4506 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 3555 3575 2761
Size: 794 20 4431
Fragments arranged by size:
4431 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5210
DraI TTT'AAA
Cuts at: 2549 2549
Size: 5245
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4252 809
Size: 507 2936 1802
Fragments arranged by size:
2936 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2131 2147 2177
Size: 54 1425 50 275 18 301 16 30
Cuts at: 2177 2692 3581 5210 8
Size: 515 889 1629 43
Fragments arranged by size:
1629 1425 889 515 301 275 54 50
43 30 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2692 1537
Size: 275 880 4090
Fragments arranged by size:
4090 880 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2504 3175 1353
Size: 1151 671 3423
Fragments arranged by size:
3423 1151 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2426 3832 4206 4352 387
Size: 1064 138 837 1406 374 146 1280
Fragments arranged by size:
1406 1280 1064 837 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2464 3308 3905 4692 4960 1239
Size: 393 557 275 844 597 787 268 1524
Fragments arranged by size:
1524 844 787 597 557 393 275 268
EcoO109I rG'GnC_Cy
Cuts at: 2032 2033 2032
Size: 1 5244
EcoRI G'AATT_C
Cuts at: 2554 2932 2554
Size: 378 4867
FspI TGC'GCA
Cuts at: 3751 3751
Size: 5245
HaeII r_GCGC'y
Cuts at: 1678 4392 1678
Size: 2714 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3886 3918 3960 3992 1373
Size: 32 135 32 2314 32 42 32 2626
Fragments arranged by size:
2626 2314 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2589 2835 669
Size: 988 281 651 246 3079
Fragments arranged by size:
3079 988 651 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5245
HpaI GTT'AAC
Cuts at: 2835 2835
Size: 5245
KpnI G_GTAC'C
Cuts at: 2570 2570
Size: 5245
MfeI C'AATT_G
Cuts at: 2844 2844
Size: 5245
MluI A'CGCG_T
Cuts at: 5121 5121
Size: 5245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4359 4543 1305
Size: 3054 184 2007
Fragments arranged by size:
3054 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2133 2149 10
Size: 54 1425 343 301 16 3106
Fragments arranged by size:
3106 1425 343 301 54 16
MslI CAynn'nnrTG
Cuts at: 518 1166 3203 3562 3721 518
Size: 648 2037 359 159 2042
Fragments arranged by size:
2042 2037 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2484 2661 3338 4486 4731 1606
Size: 409 469 177 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 469 409 245 177
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3754
Fragments arranged by size:
3754 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5245
NotI GC'GGCC_GC
Cuts at: 2692 2692
Size: 5245
NruI TCG'CGA
Cuts at: 2052 2052
Size: 5245
NspI r_CATG'y
Cuts at: 1132 1229 2603 4148 1132
Size: 97 1374 1545 2229
Fragments arranged by size:
2229 1545 1374 97
PasI CC'CwG_GG
Cuts at: 1348 2299 1348
Size: 951 4294
PciI A'CATG_T
Cuts at: 4144 4144
Size: 5245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5245
PmeI GTTT'AAAC
Cuts at: 2549 2549
Size: 5245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 4827 4859 3314
Size: 32 1481 32 3700
Fragments arranged by size:
3700 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5245
PsiI TTA'TAA
Cuts at: 2815 2815
Size: 5245
PspOMI G'GGCC_C
Cuts at: 2032 2032
Size: 5245
PstI C_TGCA'G
Cuts at: 830 2597 830
Size: 1767 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5245
PvuII CAG'CTG
Cuts at: 2484 2484
Size: 5245
SalI G'TCGA_C
Cuts at: 1936 2587 1936
Size: 651 4594
SbfI CC_TGCA'GG
Cuts at: 2597 2597
Size: 5245
ScaI AGT'ACT
Cuts at: 3493 5205 3493
Size: 1712 3533
SfcI C'TryA_G
Cuts at: 826 1045 2593 3728 4409 4600 826
Size: 219 1548 1135 681 191 1471
Fragments arranged by size:
1548 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5245
SmaI CCC'GGG
Cuts at: 1872 2572 1872
Size: 700 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3349 4250 4512 820
Size: 197 926 1406 901 262 1553
Fragments arranged by size:
1553 1406 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3036 493
Size: 2543 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5245
SphI G_CATG'C
Cuts at: 1132 2603 1132
Size: 1471 3774
SspI AAT'ATT
Cuts at: 5 52 3169 5
Size: 47 3117 2081
Fragments arranged by size:
3117 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5245
StyI C'CwwG_G
Cuts at: 513 1065 2004 2684 513
Size: 552 939 680 3074
Fragments arranged by size:
3074 939 680 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2487 3272 3289 3442 3627 4072 325
Size: 1255 907 785 17 153 185 445 1498
Fragments arranged by size:
1498 1255 907 785 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 3491 5203
Size: 274 80 33 51 1443 162 1352 1712
Cuts at: 5203 96
Size: 138
Fragments arranged by size:
1712 1443 1352 274 162 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2462 2494 5205 5237 2462
Size: 32 2711 32 2470
Fragments arranged by size:
2711 2470 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2466 1316
Size: 1150 4095
XbaI T'CTAG_A
Cuts at: 2581 2581
Size: 5245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 2481 2372
Size: 109 5136
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5245
XmaI C'CCGG_G
Cuts at: 1870 2570 1870
Size: 700 4545
XmnI GAAnn'nnTTC
Cuts at: 3374 3374
Size: 5245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3053 276
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI