Restriction map for
pFN21AB6874
(Circular) (Six-base) MAPSORT of: pFN21AB6874.seq Check: 7334 from: 1 to: 5299
pFN21AB6874 5299 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3109 278
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2642 1937
Size: 705 4594
Acc65I G'GTAC_C
Cuts at: 2620 2620
Size: 5299
AclI AA'CG_TT
Cuts at: 1563 3426 3799 1563
Size: 1863 373 3063
Fragments arranged by size:
3063 1863 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2253 3362 4746 5014 1632
Size: 621 1109 1384 268 1917
Fragments arranged by size:
1917 1384 1109 621 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5102
AflIII A'CryG_T
Cuts at: 1203 4198 5175 1203
Size: 2995 977 1327
Fragments arranged by size:
2995 1327 977
AgeI A'CCGG_T
Cuts at: 4130 4130
Size: 5299
AhdI GACnn_n'nnGTC
Cuts at: 4028 4028
Size: 5299
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5299
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5299
AlwNI CAG_nnn'CTG
Cuts at: 2288 3042 4614 2288
Size: 754 1572 2973
Fragments arranged by size:
2973 1572 754
ApaI G_GGCC'C
Cuts at: 2157 2157
Size: 5299
ApaLI G'TGCA_C
Cuts at: 3356 4512 3356
Size: 1156 4143
ApoI r'AATT_y
Cuts at: 1493 2608 2834 2986 5095 1493
Size: 1115 226 152 2109 1697
Fragments arranged by size:
2109 1697 1115 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3853 160
Size: 872 2821 1606
Fragments arranged by size:
2821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
AvaI C'yCGr_G
Cuts at: 1870 1943 2156 2624 1870
Size: 73 213 468 4545
Fragments arranged by size:
4545 468 213 73
BamHI G'GATC_C
Cuts at: 2629 2996 2629
Size: 367 4932
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2251 2620 4075 618
Size: 325 217 627 464 369 1455 1842
Fragments arranged by size:
1842 1455 627 464 369 325 217
BanII G_rGCy'C
Cuts at: 1391 2128 2157 2618 1391
Size: 737 29 461 4072
Fragments arranged by size:
4072 737 461 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2220 2482 928
Size: 971 321 262 3745
Fragments arranged by size:
3745 971 321 262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3490 3524 1903
Size: 34 1553 34 3678
Fragments arranged by size:
3678 1553 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3191 4401 3191
Size: 1210 4089
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5299
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3910 136
Size: 107 122 71 631 605 2238 1525
Fragments arranged by size:
2238 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2197 5294 2197
Size: 3097 2202
BlpI GC'TnA_GC
Cuts at: 2716 2716
Size: 5299
Bme1580I G_kGCm'C
Cuts at: 1790 2157 3360 4516 1790
Size: 367 1203 1156 2573
Fragments arranged by size:
2573 1203 1156 367
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5299
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3030 3988 442
Size: 416 53 83 399 24 1613 958 1753
Fragments arranged by size:
1753 1613 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2214 3959 1239
Size: 975 1745 2579
Fragments arranged by size:
2579 1745 975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3424 4289 4587 3424
Size: 865 298 4136
Fragments arranged by size:
4136 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3962 882
Size: 617 2463 2219
Fragments arranged by size:
2463 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3090 493
Size: 711 1886 2702
Fragments arranged by size:
2702 1886 711
BsaBI GATnn'nnATC
Cuts at: 5293 5293
Size: 5299
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3106 3488 4123 275
Size: 53 83 186 936 1573 382 635 1451
Fragments arranged by size:
1573 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2120 3732 4130 4404 4551 4962 2120
Size: 1612 398 274 147 411 2457
Fragments arranged by size:
2457 1612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5074
Fragments arranged by size:
5074 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 1186
Size: 1153 4146
BseYI C'CCAG_C
Cuts at: 4502 4998 4502
Size: 496 4803
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 1163
Size: 573 693 4033
Fragments arranged by size:
4033 693 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2749 3510 4538 1540
Size: 275 186 748 761 1028 2301
Fragments arranged by size:
2301 1028 761 748 275 186
BsiHKAI G_wGCw'C
Cuts at: 3360 3445 4516 3360
Size: 85 1071 4143
Fragments arranged by size:
4143 1071 85
BsmI GAATG_Cn'
Cuts at: 2810 2903 2810
Size: 93 5206
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2157 2618 3360 3445 4516 1391
Size: 399 338 29 461 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 461 399 338 85 29
BspEI T'CCGG_A
Cuts at: 2120 4962 2120
Size: 2842 2457
BspHI T'CATG_A
Cuts at: 3188 4918 3188
Size: 1730 3569
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2654 844
Size: 1810 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3186 1394
Size: 429 1363 3507
Fragments arranged by size:
3507 1363 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3794 3968 65
Size: 1183 2546 174 1396
Fragments arranged by size:
2546 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3943 4130 1700
Size: 110 2133 187 2869
Fragments arranged by size:
2869 2133 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5299
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5299
BssSI C'ACGA_G
Cuts at: 3359 4371 3359
Size: 1012 4287
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5299
BstBI TT'CG_AA
Cuts at: 4103 4103
Size: 5299
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5299
BstYI r'GATC_y
Cuts at: 1972 2197 2629 2996 3381 3398 4850 4936 4948
Size: 225 432 367 385 17 1452 86 12
Cuts at: 4948 5294 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 432 385 367 346 225 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3808
Fragments arranged by size:
3808 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4560
Fragments arranged by size:
4560 683 56
BtsI GCAGTG_nn'
Cuts at: 2815 3609 3629 2815
Size: 794 20 4485
Fragments arranged by size:
4485 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5299
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5264
DraI TTT'AAA
Cuts at: 2603 2603
Size: 5299
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4306 809
Size: 507 2990 1802
Fragments arranged by size:
2990 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2746 3635 5264
Size: 54 1425 50 275 18 916 889 1629
Cuts at: 5264 8
Size: 43
Fragments arranged by size:
1629 1425 916 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2746 1537
Size: 275 934 4090
Fragments arranged by size:
4090 934 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 2358 3229 1353
Size: 692 313 871 3423
Fragments arranged by size:
3423 871 692 313
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2064 3886 4260 4406 387
Size: 1064 138 475 1822 374 146 1280
Fragments arranged by size:
1822 1280 1064 475 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2253 3362 3959 4746 5014 1239
Size: 393 582 39 1109 597 787 268 1524
Fragments arranged by size:
1524 1109 787 597 582 393 268 39
EcoO109I rG'GnC_Cy
Cuts at: 2095 2153 2440 2095
Size: 58 287 4954
Fragments arranged by size:
4954 287 58
EcoRI G'AATT_C
Cuts at: 2608 2986 2608
Size: 378 4921
FspI TGC'GCA
Cuts at: 3805 3805
Size: 5299
HaeII r_GCGC'y
Cuts at: 1678 4446 1678
Size: 2768 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 3940 3972 4014
Size: 32 135 32 791 32 1545 32 42
Cuts at: 4014 4046 1373
Size: 32 2626
Fragments arranged by size:
2626 1545 791 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2643 2889 669
Size: 988 281 705 246 3079
Fragments arranged by size:
3079 988 705 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5299
HpaI GTT'AAC
Cuts at: 2889 2889
Size: 5299
KpnI G_GTAC'C
Cuts at: 2624 2624
Size: 5299
MfeI C'AATT_G
Cuts at: 2898 2898
Size: 5299
MluI A'CGCG_T
Cuts at: 5175 5175
Size: 5299
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2406 4413 4597 1305
Size: 1101 2007 184 2007
Fragments arranged by size:
2007 2007 1101 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3477
Fragments arranged by size:
3477 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3257 3616 3775 518
Size: 648 2091 359 159 2042
Fragments arranged by size:
2091 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2715 3392 4540 4785 1606
Size: 1109 677 1148 245 2120
Fragments arranged by size:
2120 1148 1109 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3808
Fragments arranged by size:
3808 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5299
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5299
NotI GC'GGCC_GC
Cuts at: 2746 2746
Size: 5299
NspI r_CATG'y
Cuts at: 1132 1229 2657 4202 1132
Size: 97 1428 1545 2229
Fragments arranged by size:
2229 1545 1428 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5299
PciI A'CATG_T
Cuts at: 4198 4198
Size: 5299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 1108
Size: 1140 4159
PmeI GTTT'AAAC
Cuts at: 2603 2603
Size: 5299
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 4881 4913 3368
Size: 32 1481 32 3754
Fragments arranged by size:
3754 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2095 2440 2095
Size: 345 4954
PshAI GACnn'nnGTC
Cuts at: 1613 2078 1613
Size: 465 4834
PsiI TTA'TAA
Cuts at: 2869 2869
Size: 5299
PspOMI G'GGCC_C
Cuts at: 2153 2153
Size: 5299
PstI C_TGCA'G
Cuts at: 830 2651 830
Size: 1821 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5299
SalI G'TCGA_C
Cuts at: 1936 2641 1936
Size: 705 4594
SapI GCTCTTCn'nnn_
Cuts at: 2045 2045
Size: 5299
SbfI CC_TGCA'GG
Cuts at: 2651 2651
Size: 5299
ScaI AGT'ACT
Cuts at: 3547 5259 3547
Size: 1712 3587
SfcI C'TryA_G
Cuts at: 826 1045 2647 3782 4463 4654 826
Size: 219 1602 1135 681 191 1471
Fragments arranged by size:
1602 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5299
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5299
SmaI CCC'GGG
Cuts at: 1872 2158 2626 1872
Size: 286 468 4545
Fragments arranged by size:
4545 468 286
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3403 4304 4566 820
Size: 197 926 1460 901 262 1553
Fragments arranged by size:
1553 1460 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3090 493
Size: 2597 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5299
SphI G_CATG'C
Cuts at: 1132 2657 1132
Size: 1525 3774
SrfI GCCC'GGGC
Cuts at: 2158 2158
Size: 5299
SspI AAT'ATT
Cuts at: 5 52 3223 5
Size: 47 3171 2081
Fragments arranged by size:
3171 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5299
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 2738 513
Size: 552 939 243 491 3074
Fragments arranged by size:
3074 939 552 491 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 3326 3343 3496 3681 4126 325
Size: 1255 870 876 17 153 185 445 1498
Fragments arranged by size:
1498 1255 876 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3545 5257 96
Size: 274 80 33 51 1443 1568 1712 138
Fragments arranged by size:
1712 1568 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5259 5291 5259
Size: 32 5267
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5299
XbaI T'CTAG_A
Cuts at: 2257 2635 2257
Size: 378 4921
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5299
XmaI C'CCGG_G
Cuts at: 1870 2156 2624 1870
Size: 286 468 4545
Fragments arranged by size:
4545 468 286
XmnI GAAnn'nnTTC
Cuts at: 3428 3428
Size: 5299
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3107 276
Size: 53 83 186 936 1573 2468
Fragments arranged by size:
2468 1573 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SwaI XcmI