Restriction map for
pFN21AB6868
(Circular) (Six-base) MAPSORT of: pFN21AB6868.seq Check: 9749 from: 1 to: 5293
pFN21AB6868 5293 bp
With 182 enzymes: SgfI *
March 28, 2012 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2498 2498
Size: 5293
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3103 278
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2636 1937
Size: 699 4594
Acc65I G'GTAC_C
Cuts at: 2614 2614
Size: 5293
AclI AA'CG_TT
Cuts at: 1563 3420 3793 1563
Size: 1857 373 3063
Fragments arranged by size:
3063 1857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3356 4740 5008 1632
Size: 1724 1384 268 1917
Fragments arranged by size:
1917 1724 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5096
AflIII A'CryG_T
Cuts at: 1203 4192 5169 1203
Size: 2989 977 1327
Fragments arranged by size:
2989 1327 977
AgeI A'CCGG_T
Cuts at: 4124 4124
Size: 5293
AhdI GACnn_n'nnGTC
Cuts at: 4022 4022
Size: 5293
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5293
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2532 2564 2532
Size: 32 5261
AlwNI CAG_nnn'CTG
Cuts at: 2409 3036 4608 2409
Size: 627 1572 3094
Fragments arranged by size:
3094 1572 627
ApaI G_GGCC'C
Cuts at: 2191 2403 2191
Size: 212 5081
ApaLI G'TGCA_C
Cuts at: 3350 4506 3350
Size: 1156 4137
ApoI r'AATT_y
Cuts at: 1493 2602 2828 2980 5089 1493
Size: 1109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3847 160
Size: 872 2815 1606
Fragments arranged by size:
2815 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
AvaI C'yCGr_G
Cuts at: 1870 1943 2618 1870
Size: 73 675 4545
Fragments arranged by size:
4545 675 73
BamHI G'GATC_C
Cuts at: 2243 2623 2990 2243
Size: 380 367 4546
Fragments arranged by size:
4546 380 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2154 2200 2343 2420 2614
Size: 325 217 627 367 46 143 77 194
Cuts at: 2614 4069 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 367 325 217 194 143
77 46
BanII G_rGCy'C
Cuts at: 1391 2191 2403 2612 1391
Size: 800 212 209 4072
Fragments arranged by size:
4072 800 212 209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 928
Size: 971 360 3962
Fragments arranged by size:
3962 971 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3484 3518 1903
Size: 34 1547 34 3678
Fragments arranged by size:
3678 1547 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3185 4395 3185
Size: 1210 4083
BclI T'GATC_A
Cuts at: 1548 2302 1548
Size: 754 4539
BfrBI ATG'CAT
Cuts at: 2477 2477
Size: 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3904 136
Size: 107 122 71 631 605 2232 1525
Fragments arranged by size:
2232 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5288 5288
Size: 5293
BlpI GC'TnA_GC
Cuts at: 2710 2710
Size: 5293
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2191 2205 2403 2425 3354 4510 1790
Size: 369 32 14 198 22 929 1156 2573
Fragments arranged by size:
2573 1156 929 369 198 32 22 14
BmgBI CAC'GTC
Cuts at: 1318 2334 1318
Size: 1016 4277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3024 3982 442
Size: 416 53 83 399 24 1607 958 1753
Fragments arranged by size:
1753 1607 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2601 3953 1239
Size: 1362 1352 2579
Fragments arranged by size:
2579 1362 1352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2472 3418 4283 4581 2472
Size: 946 865 298 3184
Fragments arranged by size:
3184 946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2303 3956 882
Size: 617 804 1653 2219
Fragments arranged by size:
2219 1653 804 617
BsaAI yAC'GTr
Cuts at: 493 1204 3084 493
Size: 711 1880 2702
Fragments arranged by size:
2702 1880 711
BsaBI GATnn'nnATC
Cuts at: 5287 5287
Size: 5293
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3100 3482 4117 275
Size: 53 83 186 936 1567 382 635 1451
Fragments arranged by size:
1567 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2240 3726 4124 4398 4545 4956 2240
Size: 1486 398 274 147 411 2577
Fragments arranged by size:
2577 1486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5068
Fragments arranged by size:
5068 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5293
BseYI C'CCAG_C
Cuts at: 4496 4992 4496
Size: 496 4797
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2481 2532 1163
Size: 573 745 51 3924
Fragments arranged by size:
3924 745 573 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2743 3504 4532 1540
Size: 275 186 742 761 1028 2301
Fragments arranged by size:
2301 1028 761 742 275 186
BsiHKAI G_wGCw'C
Cuts at: 2460 3354 3439 4510 2460
Size: 894 85 1071 3243
Fragments arranged by size:
3243 1071 894 85
BsmI GAATG_Cn'
Cuts at: 2804 2897 2804
Size: 93 5200
BsmBI CGTCTCn'nnnn_
Cuts at: 2340 2340
Size: 5293
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2191 2205 2403 2425 2460 2612
Size: 399 369 32 14 198 22 35 152
Cuts at: 2612 3354 3439 4510 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 198 152 85
35 32 22 14
BspEI T'CCGG_A
Cuts at: 4956 4956
Size: 5293
BspHI T'CATG_A
Cuts at: 3182 4912 3182
Size: 1730 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2498 2648 844
Size: 1654 150 3489
Fragments arranged by size:
3489 1654 150
BsrBI CCG'CTC
Cuts at: 1394 1823 3180 1394
Size: 429 1357 3507
Fragments arranged by size:
3507 1357 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2477 3788 3962 65
Size: 1183 1229 1311 174 1396
Fragments arranged by size:
1396 1311 1229 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3937 4124 1700
Size: 110 2127 187 2869
Fragments arranged by size:
2869 2127 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5293
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5293
BssSI C'ACGA_G
Cuts at: 3353 4365 3353
Size: 1012 4281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 794
Size: 1373 3920
BstBI TT'CG_AA
Cuts at: 4097 4097
Size: 5293
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5293
BstYI r'GATC_y
Cuts at: 1972 2243 2623 2990 3375 3392 4844 4930 4942
Size: 271 380 367 385 17 1452 86 12
Cuts at: 4942 5288 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 380 367 346 271 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2122 513
Size: 552 1057 3684
Fragments arranged by size:
3684 1057 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4554
Fragments arranged by size:
4554 683 56
BtsI GCAGTG_nn'
Cuts at: 2462 2519 2809 3603 3623 2462
Size: 57 290 794 20 4132
Fragments arranged by size:
4132 794 290 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2460 2495 529
Size: 35 1896 35 3327
Fragments arranged by size:
3327 1896 35 35
DraI TTT'AAA
Cuts at: 2597 2597
Size: 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4300 809
Size: 507 2984 1802
Fragments arranged by size:
2984 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2055 2740
Size: 54 1425 50 275 18 207 18 685
Cuts at: 2740 3629 5258 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 685 275 207 54 50
43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2740 1537
Size: 275 928 4090
Fragments arranged by size:
4090 928 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2256 3223 1353
Size: 903 967 3423
Fragments arranged by size:
3423 967 903
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3880 4254 4400 387
Size: 1064 138 2291 374 146 1280
Fragments arranged by size:
2291 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2601 3356 3953 4740 5008 1239
Size: 393 969 755 597 787 268 1524
Fragments arranged by size:
1524 969 787 755 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2187 2274 2406 2187
Size: 87 132 5074
Fragments arranged by size:
5074 132 87
EcoRI G'AATT_C
Cuts at: 2602 2980 2602
Size: 378 4915
FspI TGC'GCA
Cuts at: 3799 3799
Size: 5293
HaeII r_GCGC'y
Cuts at: 1678 4440 1678
Size: 2762 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 3934 3966 4008
Size: 32 135 32 800 32 1530 32 42
Cuts at: 4008 4040 1373
Size: 32 2626
Fragments arranged by size:
2626 1530 800 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2883 669
Size: 988 281 699 246 3079
Fragments arranged by size:
3079 988 699 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5293
HpaI GTT'AAC
Cuts at: 2883 2883
Size: 5293
KpnI G_GTAC'C
Cuts at: 2618 2618
Size: 5293
MfeI C'AATT_G
Cuts at: 2892 2892
Size: 5293
MluI A'CGCG_T
Cuts at: 5169 5169
Size: 5293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4407 4591 1305
Size: 3102 184 2007
Fragments arranged by size:
3102 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2039 2057 10
Size: 54 1425 343 207 18 3246
Fragments arranged by size:
3246 1425 343 207 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2358 3251 3610 3769 518
Size: 648 843 349 893 359 159 2042
Fragments arranged by size:
2042 893 843 648 359 349 159
MspA1I CmG'CkG
Cuts at: 1606 2441 2709 3386 4534 4779 1606
Size: 835 268 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 677 268 245
NcoI C'CATG_G
Cuts at: 513 1065 2122 513
Size: 552 1057 3684
Fragments arranged by size:
3684 1057 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5293
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5293
NotI GC'GGCC_GC
Cuts at: 2740 2740
Size: 5293
NsiI A_TGCA'T
Cuts at: 2479 2479
Size: 5293
NspI r_CATG'y
Cuts at: 1132 1229 2477 2651 4196 1132
Size: 97 1248 174 1545 2229
Fragments arranged by size:
2229 1545 1248 174 97
PasI CC'CwG_GG
Cuts at: 1348 2403 1348
Size: 1055 4238
PciI A'CATG_T
Cuts at: 4192 4192
Size: 5293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2228 1108
Size: 1120 4173
PmeI GTTT'AAAC
Cuts at: 2597 2597
Size: 5293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 4875 4907 3362
Size: 32 1481 32 3748
Fragments arranged by size:
3748 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2274 2274
Size: 5293
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5293
PsiI TTA'TAA
Cuts at: 2863 2863
Size: 5293
PspOMI G'GGCC_C
Cuts at: 2187 2399 2187
Size: 212 5081
PstI C_TGCA'G
Cuts at: 830 2084 2395 2645 830
Size: 1254 311 250 3478
Fragments arranged by size:
3478 1254 311 250
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5293
SalI G'TCGA_C
Cuts at: 1936 2635 1936
Size: 699 4594
SbfI CC_TGCA'GG
Cuts at: 2645 2645
Size: 5293
ScaI AGT'ACT
Cuts at: 3541 5253 3541
Size: 1712 3581
SfcI C'TryA_G
Cuts at: 826 1045 2080 2391 2410 2641 3776 4457 4648
Size: 219 1035 311 19 231 1135 681 191
Cuts at: 4648 826
Size: 1471
Fragments arranged by size:
1471 1135 1035 681 311 231 219 191
19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5293
SmaI CCC'GGG
Cuts at: 1872 2620 1872
Size: 748 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2487 3397 4298 4560 820
Size: 197 926 544 910 901 262 1553
Fragments arranged by size:
1553 926 910 901 544 262 197
SnaBI TAC'GTA
Cuts at: 493 3084 493
Size: 2591 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5293
SphI G_CATG'C
Cuts at: 1132 2651 1132
Size: 1519 3774
SspI AAT'ATT
Cuts at: 5 52 3217 5
Size: 47 3165 2081
Fragments arranged by size:
3165 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5293
StyI C'CwwG_G
Cuts at: 513 1065 2122 2339 2732 513
Size: 552 1057 217 393 3074
Fragments arranged by size:
3074 1057 552 393 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2041 3320 3337 3490 3675 4120 325
Size: 1255 461 1279 17 153 185 445 1498
Fragments arranged by size:
1498 1279 1255 461 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3539 5251 96
Size: 274 80 33 51 1443 1562 1712 138
Fragments arranged by size:
1712 1562 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2228 2260 5253 5285 2228
Size: 32 2993 32 2236
Fragments arranged by size:
2993 2236 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5293
XbaI T'CTAG_A
Cuts at: 2629 2629
Size: 5293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5293
XmaI C'CCGG_G
Cuts at: 1870 2618 1870
Size: 748 4545
XmnI GAAnn'nnTTC
Cuts at: 3422 3422
Size: 5293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3101 276
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI