Restriction map for
pFN21AB6866
(Circular) (Six-base) MAPSORT of: pFN21AB6866.seq Check: 868 from: 1 to: 5293
pFN21AB6866 5293 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3103 278
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2636 1937
Size: 699 4594
Acc65I G'GTAC_C
Cuts at: 2614 2614
Size: 5293
AclI AA'CG_TT
Cuts at: 1563 3420 3793 1563
Size: 1857 373 3063
Fragments arranged by size:
3063 1857 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3356 4740 5008 1632
Size: 1724 1384 268 1917
Fragments arranged by size:
1917 1724 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5096
AflIII A'CryG_T
Cuts at: 1203 4192 5169 1203
Size: 2989 977 1327
Fragments arranged by size:
2989 1327 977
AgeI A'CCGG_T
Cuts at: 4124 4124
Size: 5293
AhdI GACnn_n'nnGTC
Cuts at: 2138 4022 2138
Size: 1884 3409
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5293
AlwNI CAG_nnn'CTG
Cuts at: 3036 4608 3036
Size: 1572 3721
ApaI G_GGCC'C
Cuts at: 2495 2542 2495
Size: 47 5246
ApaLI G'TGCA_C
Cuts at: 3350 4506 3350
Size: 1156 4137
ApoI r'AATT_y
Cuts at: 1493 2602 2828 2980 5089 1493
Size: 1109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1109 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3847 160
Size: 872 2815 1606
Fragments arranged by size:
2815 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
AvaI C'yCGr_G
Cuts at: 1870 1943 2488 2495 2541 2618 1870
Size: 73 545 7 46 77 4545
Fragments arranged by size:
4545 545 77 73 46 7
BamHI G'GATC_C
Cuts at: 2335 2418 2623 2990 2335
Size: 83 205 367 4638
Fragments arranged by size:
4638 367 205 83
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2614 4069 618
Size: 325 217 627 827 1455 1842
Fragments arranged by size:
1842 1455 827 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2495 2508 2542 2612 1391
Size: 1104 13 34 70 4072
Fragments arranged by size:
4072 1104 70 34 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3484 3518 1903
Size: 34 1547 34 3678
Fragments arranged by size:
3678 1547 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3185 4395 3185
Size: 1210 4083
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5293
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3904 136
Size: 107 122 71 631 605 2232 1525
Fragments arranged by size:
2232 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5288 5288
Size: 5293
BlpI GC'TnA_GC
Cuts at: 2710 2710
Size: 5293
Bme1580I G_kGCm'C
Cuts at: 1790 2495 2527 2542 3354 4510 1790
Size: 705 32 15 812 1156 2573
Fragments arranged by size:
2573 1156 812 705 32 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5293
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2111 2191 3024
Size: 416 53 83 399 24 694 80 833
Cuts at: 3024 3982 442
Size: 958 1753
Fragments arranged by size:
1753 958 833 694 416 399 83 80
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2265 3953 1239
Size: 1026 1688 2579
Fragments arranged by size:
2579 1688 1026
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2295 3418 4283 4581 2295
Size: 1123 865 298 3007
Fragments arranged by size:
3007 1123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3956 882
Size: 617 2457 2219
Fragments arranged by size:
2457 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3084 493
Size: 711 1880 2702
Fragments arranged by size:
2702 1880 711
BsaBI GATnn'nnATC
Cuts at: 5287 5287
Size: 5293
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3100 3482 4117 275
Size: 53 83 186 936 1567 382 635 1451
Fragments arranged by size:
1567 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3726 4124 4398 4545 4956 3726
Size: 398 274 147 411 4063
Fragments arranged by size:
4063 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2238 2268 2363 2393 1178
Size: 30 165 30 835 30 95 30 4078
Fragments arranged by size:
4078 835 165 95 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2384 2397 1186
Size: 1198 13 4082
Fragments arranged by size:
4082 1198 13
BseYI C'CCAG_C
Cuts at: 4496 4992 4496
Size: 496 4797
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2068 2169 1163
Size: 573 332 101 4287
Fragments arranged by size:
4287 573 332 101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2743 3504 4532 1540
Size: 275 186 742 761 1028 2301
Fragments arranged by size:
2301 1028 761 742 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 3354 3439 4510 2508
Size: 846 85 1071 3291
Fragments arranged by size:
3291 1071 846 85
BsmI GAATG_Cn'
Cuts at: 2804 2897 2804
Size: 93 5200
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2495 2508 2527 2542 2612 3354 3439
Size: 399 705 13 19 15 70 742 85
Cuts at: 3439 4510 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 705 399 85 70 19
15 13
BspEI T'CCGG_A
Cuts at: 4956 4956
Size: 5293
BspHI T'CATG_A
Cuts at: 3182 4912 3182
Size: 1730 3563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2289 2648 844
Size: 1445 359 3489
Fragments arranged by size:
3489 1445 359
BsrBI CCG'CTC
Cuts at: 1394 1823 3180 1394
Size: 429 1357 3507
Fragments arranged by size:
3507 1357 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3788 3962 65
Size: 1183 2540 174 1396
Fragments arranged by size:
2540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3937 4124 1700
Size: 110 2127 187 2869
Fragments arranged by size:
2869 2127 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5293
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5293
BssSI C'ACGA_G
Cuts at: 3353 4365 3353
Size: 1012 4281
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5293
BstBI TT'CG_AA
Cuts at: 4097 4097
Size: 5293
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5293
BstYI r'GATC_y
Cuts at: 1972 2089 2335 2418 2623 2990 3375 3392 4844
Size: 117 246 83 205 367 385 17 1452
Cuts at: 4844 4930 4942 5288 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 246 205 117
86 83 17 12
Bsu36I CC'TnA_GG
Cuts at: 2409 2409
Size: 5293
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4741
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4554
Fragments arranged by size:
4554 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 2809 3603 3623 2040
Size: 769 794 20 3710
Fragments arranged by size:
3710 794 769 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5258
DraI TTT'AAA
Cuts at: 2597 2597
Size: 5293
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4300 809
Size: 507 2984 1802
Fragments arranged by size:
2984 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 3629 5258
Size: 54 1425 50 275 18 910 889 1629
Cuts at: 5258 8
Size: 43
Fragments arranged by size:
1629 1425 910 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2740 1537
Size: 275 928 4090
Fragments arranged by size:
4090 928 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 3223 1353
Size: 900 970 3423
Fragments arranged by size:
3423 970 900
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3880 4254 4400 387
Size: 1064 138 2291 374 146 1280
Fragments arranged by size:
2291 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2506 2506
Size: 5293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 3356 3953 4740 5008 1239
Size: 393 633 1091 597 787 268 1524
Fragments arranged by size:
1524 1091 787 633 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2140 2263 2351 2492 2538 2140
Size: 123 88 141 46 4895
Fragments arranged by size:
4895 141 123 88 46
EcoRI G'AATT_C
Cuts at: 2602 2980 2602
Size: 378 4915
FspI TGC'GCA
Cuts at: 3799 3799
Size: 5293
HaeII r_GCGC'y
Cuts at: 1678 4440 1678
Size: 2762 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2124 2156 2410 2442 2462
Size: 32 135 32 552 32 254 32 20
Cuts at: 2462 2494 3934 3966 4008 4040 1373
Size: 32 1440 32 42 32 2626
Fragments arranged by size:
2626 1440 552 254 135 42 32 32
32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 2637 2883 669
Size: 988 281 699 246 3079
Fragments arranged by size:
3079 988 699 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5293
HpaI GTT'AAC
Cuts at: 2883 2883
Size: 5293
KpnI G_GTAC'C
Cuts at: 2618 2618
Size: 5293
MfeI C'AATT_G
Cuts at: 2892 2892
Size: 5293
MluI A'CGCG_T
Cuts at: 5169 5169
Size: 5293
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2363 2396 4407 4591 1305
Size: 1058 33 2011 184 2007
Fragments arranged by size:
2011 2007 1058 184 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3471
Fragments arranged by size:
3471 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3251 3610 3769 518
Size: 648 2085 359 159 2042
Fragments arranged by size:
2085 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2269 2398 2709 3386 4534 4779 1606
Size: 663 129 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 663 311 245 129
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4741
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5293
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5293
NotI GC'GGCC_GC
Cuts at: 2740 2740
Size: 5293
NspI r_CATG'y
Cuts at: 1132 1229 2651 4196 1132
Size: 97 1422 1545 2229
Fragments arranged by size:
2229 1545 1422 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5293
PciI A'CATG_T
Cuts at: 4192 4192
Size: 5293
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2345 1108
Size: 1237 4056
PfoI T'CCnGG_A
Cuts at: 2428 2553 2428
Size: 125 5168
PmeI GTTT'AAAC
Cuts at: 2597 2597
Size: 5293
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 4875 4907 3362
Size: 32 1481 32 3748
Fragments arranged by size:
3748 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2263 2140
Size: 123 5170
PshAI GACnn'nnGTC
Cuts at: 1613 2305 1613
Size: 692 4601
PsiI TTA'TAA
Cuts at: 2863 2863
Size: 5293
PspOMI G'GGCC_C
Cuts at: 2491 2538 2491
Size: 47 5246
PstI C_TGCA'G
Cuts at: 830 2062 2368 2397 2587 2645 830
Size: 1232 306 29 190 58 3478
Fragments arranged by size:
3478 1232 306 190 58 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5293
PvuII CAG'CTG
Cuts at: 2398 2398
Size: 5293
SacI G_AGCT'C
Cuts at: 2508 2508
Size: 5293
SalI G'TCGA_C
Cuts at: 1936 2635 1936
Size: 699 4594
SbfI CC_TGCA'GG
Cuts at: 2645 2645
Size: 5293
ScaI AGT'ACT
Cuts at: 2447 3541 5253 2447
Size: 1094 1712 2487
Fragments arranged by size:
2487 1712 1094
SfcI C'TryA_G
Cuts at: 826 1045 2058 2291 2364 2393 2583 2641 3776
Size: 219 1013 233 73 29 190 58 1135
Cuts at: 3776 4457 4648 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1013 681 233 219 191 190
73 58 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5293
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5293
SmaI CCC'GGG
Cuts at: 1872 2543 2620 1872
Size: 671 77 4545
Fragments arranged by size:
4545 671 77
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2310 3397 4298 4560 820
Size: 197 926 367 1087 901 262 1553
Fragments arranged by size:
1553 1087 926 901 367 262 197
SnaBI TAC'GTA
Cuts at: 493 3084 493
Size: 2591 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5293
SphI G_CATG'C
Cuts at: 1132 2651 1132
Size: 1519 3774
SrfI GCCC'GGGC
Cuts at: 2543 2543
Size: 5293
SspI AAT'ATT
Cuts at: 5 52 3217 5
Size: 47 3165 2081
Fragments arranged by size:
3165 2081 47
StuI AGG'CCT
Cuts at: 1517 2126 2486 2564 1517
Size: 609 360 78 4246
Fragments arranged by size:
4246 609 360 78
StyI C'CwwG_G
Cuts at: 513 1065 2732 513
Size: 552 1667 3074
Fragments arranged by size:
3074 1667 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3320 3337 3490 3675 4120 325
Size: 1255 1740 17 153 185 445 1498
Fragments arranged by size:
1740 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2445 3539 5251
Size: 274 80 33 51 1443 468 1094 1712
Cuts at: 5251 96
Size: 138
Fragments arranged by size:
1712 1443 1094 468 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2091 2123 2238 2270 2363 2395 5253 5285 2091
Size: 32 115 32 93 32 2858 32 2099
Fragments arranged by size:
2858 2099 115 93 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5293
XbaI T'CTAG_A
Cuts at: 2629 2629
Size: 5293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5293
XmaI C'CCGG_G
Cuts at: 1870 2541 2618 1870
Size: 671 77 4545
Fragments arranged by size:
4545 671 77
XmnI GAAnn'nnTTC
Cuts at: 3422 3422
Size: 5293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3101 276
Size: 53 83 186 936 1567 2468
Fragments arranged by size:
2468 1567 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI