Restriction map for
pFN21AB6841
(Circular) (Six-base) MAPSORT of: pFN21AB6841.seq Check: 8679 from: 1 to: 5221
pFN21AB6841 5221 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2307 2382 2307
Size: 75 5146
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3031 278
Size: 53 83 186 936 1495 2468
Fragments arranged by size:
2468 1495 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2564 1937
Size: 627 4594
Acc65I G'GTAC_C
Cuts at: 2542 2542
Size: 5221
AclI AA'CG_TT
Cuts at: 1563 3348 3721 1563
Size: 1785 373 3063
Fragments arranged by size:
3063 1785 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3284 4668 4936 1632
Size: 1652 1384 268 1917
Fragments arranged by size:
1917 1652 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5024
AflIII A'CryG_T
Cuts at: 1203 2012 4120 5097 1203
Size: 809 2108 977 1327
Fragments arranged by size:
2108 1327 977 809
AgeI A'CCGG_T
Cuts at: 4052 4052
Size: 5221
AhdI GACnn_n'nnGTC
Cuts at: 2464 3950 2464
Size: 1486 3735
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5221
AlwNI CAG_nnn'CTG
Cuts at: 2163 2383 2964 4536 2163
Size: 220 581 1572 2848
Fragments arranged by size:
2848 1572 581 220
ApaLI G'TGCA_C
Cuts at: 3278 4434 3278
Size: 1156 4065
ApoI r'AATT_y
Cuts at: 1493 2530 2756 2908 5017 1493
Size: 1037 226 152 2109 1697
Fragments arranged by size:
2109 1697 1037 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3775 160
Size: 872 2743 1606
Fragments arranged by size:
2743 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5221
AvaI C'yCGr_G
Cuts at: 1870 1943 2304 2408 2504 2546 1870
Size: 73 361 104 96 42 4545
Fragments arranged by size:
4545 361 104 96 73 42
BamHI G'GATC_C
Cuts at: 2551 2918 2551
Size: 367 4854
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2423 2482 2542 3997 618
Size: 325 217 627 636 59 60 1455 1842
Fragments arranged by size:
1842 1455 636 627 325 217 60 59
BanII G_rGCy'C
Cuts at: 1391 2217 2409 2505 2540 1391
Size: 826 192 96 35 4072
Fragments arranged by size:
4072 826 192 96 35
BbeI G_GCGC'C
Cuts at: 2427 2486 2427
Size: 59 5162
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4250
BbvCI CC'TCA_GC
Cuts at: 2327 2327
Size: 5221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2261 2295 3412 3446 1903
Size: 34 324 34 1117 34 3678
Fragments arranged by size:
3678 1117 324 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3113 4323 3113
Size: 1210 4011
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5221
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2480 3832 136
Size: 107 122 71 631 605 808 1352 1525
Fragments arranged by size:
1525 1352 808 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5216 5216
Size: 5221
BlpI GC'TnA_GC
Cuts at: 2638 2638
Size: 5221
Bme1580I G_kGCm'C
Cuts at: 1790 3282 4438 1790
Size: 1492 1156 2573
Fragments arranged by size:
2573 1492 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5221
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2952 3910 442
Size: 416 53 83 399 24 1535 958 1753
Fragments arranged by size:
1753 1535 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 3881 1239
Size: 1169 1473 2579
Fragments arranged by size:
2579 1473 1169
Bpu10I CC'TnA_GC
Cuts at: 2327 2327
Size: 5221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3346 4211 4509 3346
Size: 865 298 4058
Fragments arranged by size:
4058 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2286 3884 882
Size: 617 787 1598 2219
Fragments arranged by size:
2219 1598 787 617
BsaAI yAC'GTr
Cuts at: 493 1204 3012 493
Size: 711 1808 2702
Fragments arranged by size:
2702 1808 711
BsaBI GATnn'nnATC
Cuts at: 5215 5215
Size: 5221
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2424 2483 3028
Size: 53 83 186 936 600 291 59 545
Cuts at: 3028 3410 4045 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 635 600 545 382 291 186
83 59 53
BsaWI w'CCGG_w
Cuts at: 3654 4052 4326 4473 4884 3654
Size: 398 274 147 411 3991
Fragments arranged by size:
3991 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4996
Fragments arranged by size:
4996 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 1186
Size: 1336 3885
BseYI C'CCAG_C
Cuts at: 2142 2433 2492 4424 4920 2142
Size: 291 59 1932 496 2443
Fragments arranged by size:
2443 1932 496 291 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4648
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2421 2671 3432 4460 1540
Size: 275 186 420 250 761 1028 2301
Fragments arranged by size:
2301 1028 761 420 275 250 186
BsiHKAI G_wGCw'C
Cuts at: 3282 3367 4438 3282
Size: 85 1071 4065
Fragments arranged by size:
4065 1071 85
BsmI GAATG_Cn'
Cuts at: 2453 2732 2825 2453
Size: 279 93 4849
Fragments arranged by size:
4849 279 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2333 2003
Size: 330 4891
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2409 2505 2540 3282 3367 4438
Size: 399 427 192 96 35 742 85 1071
Cuts at: 4438 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 427 399 192 96 85
35
BspEI T'CCGG_A
Cuts at: 4884 4884
Size: 5221
BspHI T'CATG_A
Cuts at: 3110 4840 3110
Size: 1730 3491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2307 2382 2576 844
Size: 1463 75 194 3489
Fragments arranged by size:
3489 1463 194 75
BsrBI CCG'CTC
Cuts at: 1394 1823 2248 3108 1394
Size: 429 425 860 3507
Fragments arranged by size:
3507 860 429 425
BsrDI GCAATG_nn'
Cuts at: 65 1248 3716 3890 65
Size: 1183 2468 174 1396
Fragments arranged by size:
2468 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2420 3865 4052 1700
Size: 110 210 400 1445 187 2869
Fragments arranged by size:
2869 1445 400 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5221
BssHII G'CGCG_C
Cuts at: 1925 2153 2282 1925
Size: 228 129 4864
Fragments arranged by size:
4864 228 129
BssSI C'ACGA_G
Cuts at: 3281 4293 3281
Size: 1012 4209
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2163 2185 2257 2383 794
Size: 1369 22 72 126 3632
Fragments arranged by size:
3632 1369 126 72 22
BstBI TT'CG_AA
Cuts at: 4025 4025
Size: 5221
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5221
BstYI r'GATC_y
Cuts at: 1972 2551 2918 3303 3320 4772 4858 4870 5216
Size: 579 367 385 17 1452 86 12 346
Cuts at: 5216 1972
Size: 1977
Fragments arranged by size:
1977 1452 579 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2111 2111
Size: 5221
BtgI C'CryG_G
Cuts at: 513 1065 2004 2096 513
Size: 552 939 92 3638
Fragments arranged by size:
3638 939 552 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4482
Fragments arranged by size:
4482 683 56
BtsI GCAGTG_nn'
Cuts at: 2737 3531 3551 2737
Size: 794 20 4407
Fragments arranged by size:
4407 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5221
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5186
DraI TTT'AAA
Cuts at: 2525 2525
Size: 5221
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4228 809
Size: 507 2912 1802
Fragments arranged by size:
2912 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 2668 3557
Size: 54 1425 50 275 18 588 250 889
Cuts at: 3557 5186 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 588 275 250 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2418 2668 1537
Size: 275 606 250 4090
Fragments arranged by size:
4090 606 275 250
EarI CTCTTCn'nnn_
Cuts at: 1353 2482 3151 1353
Size: 1129 669 3423
Fragments arranged by size:
3423 1129 669
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 2459 3808 4182 4328 387
Size: 1064 138 450 420 1349 374 146 1280
Fragments arranged by size:
1349 1280 1064 450 420 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 3284 3881 4668 4936 1239
Size: 393 776 876 597 787 268 1524
Fragments arranged by size:
1524 876 787 776 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2079 2104 2079
Size: 25 5196
EcoRI G'AATT_C
Cuts at: 2530 2908 2530
Size: 378 4843
FspI TGC'GCA
Cuts at: 2054 2167 3727 2054
Size: 113 1560 3548
Fragments arranged by size:
3548 1560 113
HaeII r_GCGC'y
Cuts at: 1678 2208 2326 2395 2427 2486 4368 1678
Size: 530 118 69 32 59 1882 2531
Fragments arranged by size:
2531 1882 530 118 69 59 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 3862 3894 3936
Size: 32 135 32 878 32 1380 32 42
Cuts at: 3936 3968 1373
Size: 32 2626
Fragments arranged by size:
2626 1380 878 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2565 2811 669
Size: 988 281 627 246 3079
Fragments arranged by size:
3079 988 627 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5221
HpaI GTT'AAC
Cuts at: 2811 2811
Size: 5221
KasI G'GCGC_C
Cuts at: 2423 2482 2423
Size: 59 5162
KpnI G_GTAC'C
Cuts at: 2546 2546
Size: 5221
MfeI C'AATT_G
Cuts at: 2820 2820
Size: 5221
MluI A'CGCG_T
Cuts at: 2012 5097 2012
Size: 3085 2136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4335 4519 1305
Size: 3030 184 2007
Fragments arranged by size:
3030 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3399
Fragments arranged by size:
3399 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2173 3179 3538 3697 518
Size: 648 1007 1006 359 159 2042
Fragments arranged by size:
2042 1007 1006 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2160 2197 2254 2637 3314 4462 4707
Size: 492 62 37 57 383 677 1148 245
Cuts at: 4707 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 492 383 245 62 57
37
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 5221
NarI GG'CG_CC
Cuts at: 2424 2483 2424
Size: 59 5162
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3730
Fragments arranged by size:
3730 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5221
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 5221
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5221
NotI GC'GGCC_GC
Cuts at: 2668 2668
Size: 5221
NspI r_CATG'y
Cuts at: 1132 1229 2579 4124 1132
Size: 97 1350 1545 2229
Fragments arranged by size:
2229 1545 1350 97
PasI CC'CwG_GG
Cuts at: 1348 2065 1348
Size: 717 4504
PciI A'CATG_T
Cuts at: 4120 4120
Size: 5221
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5221
PmeI GTTT'AAAC
Cuts at: 2525 2525
Size: 5221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 4803 4835 3290
Size: 32 1481 32 3676
Fragments arranged by size:
3676 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2079 2104 2079
Size: 25 5196
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5221
PsiI TTA'TAA
Cuts at: 2791 2791
Size: 5221
PspXI vC'TCGA_Gb
Cuts at: 2408 2408
Size: 5221
PstI C_TGCA'G
Cuts at: 830 2379 2573 830
Size: 1549 194 3478
Fragments arranged by size:
3478 1549 194
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5221
SacII CC_GC'GG
Cuts at: 2099 2099
Size: 5221
SalI G'TCGA_C
Cuts at: 1936 2563 1936
Size: 627 4594
SanDI GG'GwC_CC
Cuts at: 2079 2104 2079
Size: 25 5196
SbfI CC_TGCA'GG
Cuts at: 2379 2573 2379
Size: 194 5027
ScaI AGT'ACT
Cuts at: 2183 3469 5181 2183
Size: 1286 1712 2223
Fragments arranged by size:
2223 1712 1286
SfcI C'TryA_G
Cuts at: 826 1045 2375 2569 3704 4385 4576 826
Size: 219 1330 194 1135 681 191 1471
Fragments arranged by size:
1471 1330 1135 681 219 194 191
SfoI GGC'GCC
Cuts at: 2425 2484 2425
Size: 59 5162
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5221
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5221
SmaI CCC'GGG
Cuts at: 1872 2306 2548 1872
Size: 434 242 4545
Fragments arranged by size:
4545 434 242
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 3325 4226 4488 820
Size: 197 926 465 917 901 262 1553
Fragments arranged by size:
1553 926 917 901 465 262 197
SnaBI TAC'GTA
Cuts at: 493 3012 493
Size: 2519 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5221
SphI G_CATG'C
Cuts at: 1132 2579 1132
Size: 1447 3774
SspI AAT'ATT
Cuts at: 5 52 3145 5
Size: 47 3093 2081
Fragments arranged by size:
3093 2081 47
StuI AGG'CCT
Cuts at: 1517 2399 2474 1517
Size: 882 75 4264
Fragments arranged by size:
4264 882 75
StyI C'CwwG_G
Cuts at: 513 1065 2004 2469 2660 513
Size: 552 939 465 191 3074
Fragments arranged by size:
3074 939 552 465 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3248 3265 3418 3603 4048 325
Size: 1255 1668 17 153 185 445 1498
Fragments arranged by size:
1668 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3467 5179
Size: 274 80 33 51 1443 204 1286 1712
Cuts at: 5179 96
Size: 138
Fragments arranged by size:
1712 1443 1286 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 5181 5213 2123
Size: 32 3026 32 2131
Fragments arranged by size:
3026 2131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5221
XbaI T'CTAG_A
Cuts at: 2557 2557
Size: 5221
XhoI C'TCGA_G
Cuts at: 1943 2408 1943
Size: 465 4756
XmaI C'CCGG_G
Cuts at: 1870 2304 2546 1870
Size: 434 242 4545
Fragments arranged by size:
4545 434 242
XmnI GAAnn'nnTTC
Cuts at: 3350 3350
Size: 5221
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3029 276
Size: 53 83 186 936 1495 2468
Fragments arranged by size:
2468 1495 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI
PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI
SwaI XcmI