Restriction map for
pFN21AB6840
(Circular) (Six-base) MAPSORT of: pFN21AB6840.seq Check: 1569 from: 1 to: 5278
pFN21AB6840 5278 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2250 2250
Size: 5278
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3088 278
Size: 53 83 186 936 1552 2468
Fragments arranged by size:
2468 1552 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2621 1937
Size: 684 4594
Acc65I G'GTAC_C
Cuts at: 2599 2599
Size: 5278
AclI AA'CG_TT
Cuts at: 1563 3405 3778 1563
Size: 1842 373 3063
Fragments arranged by size:
3063 1842 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 3341 4725 4993 1632
Size: 590 1119 1384 268 1917
Fragments arranged by size:
1917 1384 1119 590 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5081
AflIII A'CryG_T
Cuts at: 1203 2244 4177 5154 1203
Size: 1041 1933 977 1327
Fragments arranged by size:
1933 1327 1041 977
AgeI A'CCGG_T
Cuts at: 2510 4109 2510
Size: 1599 3679
AhdI GACnn_n'nnGTC
Cuts at: 4007 4007
Size: 5278
AleI CACnn'nnGTG
Cuts at: 1166 2114 1166
Size: 948 4330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2231 1184
Size: 1047 4231
AlwNI CAG_nnn'CTG
Cuts at: 2554 3021 4593 2554
Size: 467 1572 3239
Fragments arranged by size:
3239 1572 467
ApaI G_GGCC'C
Cuts at: 2466 2466
Size: 5278
ApaLI G'TGCA_C
Cuts at: 3335 4491 3335
Size: 1156 4122
ApoI r'AATT_y
Cuts at: 1493 2381 2587 2813 2965 5074 1493
Size: 888 206 226 152 2109 1697
Fragments arranged by size:
2109 1697 888 226 206 152
AseI AT'TA_AT
Cuts at: 160 1032 3832 160
Size: 872 2800 1606
Fragments arranged by size:
2800 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5278
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 2352 2421 2603 1870
Size: 73 349 60 69 182 4545
Fragments arranged by size:
4545 349 182 73 69 60
BamHI G'GATC_C
Cuts at: 2608 2975 2608
Size: 367 4911
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2215 2599 4054 618
Size: 325 217 627 428 384 1455 1842
Fragments arranged by size:
1842 1455 627 428 384 325 217
BanII G_rGCy'C
Cuts at: 1391 2053 2466 2597 1391
Size: 662 413 131 4072
Fragments arranged by size:
4072 662 413 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2392 928
Size: 971 493 3814
Fragments arranged by size:
3814 971 493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2037 2071 2101 2135 3469 3503 1903
Size: 34 100 34 30 34 1334 34 3678
Fragments arranged by size:
3678 1334 100 34 34 34 34 30
BciVI GTATCCnnnnn_n'
Cuts at: 3170 4380 3170
Size: 1210 4068
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3889 136
Size: 107 122 71 631 605 2217 1525
Fragments arranged by size:
2217 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5273 5273
Size: 5278
BlpI GC'TnA_GC
Cuts at: 2695 2695
Size: 5278
Bme1580I G_kGCm'C
Cuts at: 1790 2220 2466 3339 4495 1790
Size: 430 246 873 1156 2573
Fragments arranged by size:
2573 1156 873 430 246
BmgBI CAC'GTC
Cuts at: 1318 2362 1318
Size: 1044 4234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2215 3009 3967
Size: 416 53 83 399 24 798 794 958
Cuts at: 3967 442
Size: 1753
Fragments arranged by size:
1753 958 798 794 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3938 1239
Size: 2699 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3403 4268 4566 3403
Size: 865 298 4115
Fragments arranged by size:
4115 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2168 3941 882
Size: 617 669 1773 2219
Fragments arranged by size:
2219 1773 669 617
BsaAI yAC'GTr
Cuts at: 493 1204 3069 493
Size: 711 1865 2702
Fragments arranged by size:
2702 1865 711
BsaBI GATnn'nnATC
Cuts at: 5272 5272
Size: 5278
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3085 3467 4102 275
Size: 53 83 186 936 1552 382 635 1451
Fragments arranged by size:
1552 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2510 3711 4109 4383 4530 4941 2510
Size: 1201 398 274 147 411 2847
Fragments arranged by size:
2847 1201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 1178
Size: 30 165 30 993 30 4030
Fragments arranged by size:
4030 993 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2465 2510 1186
Size: 1024 255 45 3954
Fragments arranged by size:
3954 1024 255 45
BseYI C'CCAG_C
Cuts at: 4481 4977 4481
Size: 496 4782
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4705
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2728 3489 4517 1540
Size: 275 186 727 761 1028 2301
Fragments arranged by size:
2301 1028 761 727 275 186
BsiHKAI G_wGCw'C
Cuts at: 2315 3339 3424 4495 2315
Size: 1024 85 1071 3098
Fragments arranged by size:
3098 1071 1024 85
BsmI GAATG_Cn'
Cuts at: 2789 2882 2789
Size: 93 5185
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2220 2315 2466 2597 3339 3424
Size: 399 263 167 95 151 131 742 85
Cuts at: 3424 4495 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 263 167 151 131
95 85
BspEI T'CCGG_A
Cuts at: 4941 4941
Size: 5278
BspHI T'CATG_A
Cuts at: 3167 4897 3167
Size: 1730 3548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2250 2386 2633 844
Size: 1406 136 247 3489
Fragments arranged by size:
3489 1406 247 136
BsrBI CCG'CTC
Cuts at: 1394 1823 3165 1394
Size: 429 1342 3507
Fragments arranged by size:
3507 1342 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3773 3947 65
Size: 1183 2525 174 1396
Fragments arranged by size:
2525 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2255 2510 3922 4109 1700
Size: 110 445 255 1412 187 2869
Fragments arranged by size:
2869 1412 445 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5278
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5278
BssSI C'ACGA_G
Cuts at: 2046 3338 4350 2046
Size: 1292 1012 2974
Fragments arranged by size:
2974 1292 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5278
BstBI TT'CG_AA
Cuts at: 4082 4082
Size: 5278
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5278
BstXI CCAn_nnnn'nTGG
Cuts at: 2235 2235
Size: 5278
BstYI r'GATC_y
Cuts at: 1972 2608 2975 3360 3377 4829 4915 4927 5273
Size: 636 367 385 17 1452 86 12 346
Cuts at: 5273 1972
Size: 1977
Fragments arranged by size:
1977 1452 636 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2532 513
Size: 552 939 66 462 3259
Fragments arranged by size:
3259 939 552 462 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4539
Fragments arranged by size:
4539 683 56
BtsI GCAGTG_nn'
Cuts at: 2315 2794 3588 3608 2315
Size: 479 794 20 3985
Fragments arranged by size:
3985 794 479 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5278
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5243
DraI TTT'AAA
Cuts at: 2582 2582
Size: 5278
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4285 809
Size: 507 2969 1802
Fragments arranged by size:
2969 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2725 3614 5243
Size: 54 1425 50 275 18 895 889 1629
Cuts at: 5243 8
Size: 43
Fragments arranged by size:
1629 1425 895 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2725 1537
Size: 275 913 4090
Fragments arranged by size:
4090 913 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2483 2492 2534 3208 1353
Size: 1130 9 42 674 3423
Fragments arranged by size:
3423 1130 674 42 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3865 4239 4385 387
Size: 1064 138 2276 374 146 1280
Fragments arranged by size:
2276 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 3341 3938 4725 4993 1239
Size: 393 590 1119 597 787 268 1524
Fragments arranged by size:
1524 1119 787 597 590 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2463 2470 2463
Size: 7 5271
EcoRI G'AATT_C
Cuts at: 2587 2965 2587
Size: 378 4900
FspI TGC'GCA
Cuts at: 3784 3784
Size: 5278
HaeII r_GCGC'y
Cuts at: 1678 2455 4425 1678
Size: 777 1970 2531
Fragments arranged by size:
2531 1970 777
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3919 3951 3993 4025 1373
Size: 32 135 32 2347 32 42 32 2626
Fragments arranged by size:
2626 2347 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2242 2622 2868 669
Size: 988 281 304 380 246 3079
Fragments arranged by size:
3079 988 380 304 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5278
HpaI GTT'AAC
Cuts at: 2868 2868
Size: 5278
KpnI G_GTAC'C
Cuts at: 2603 2603
Size: 5278
MfeI C'AATT_G
Cuts at: 2877 2877
Size: 5278
MluI A'CGCG_T
Cuts at: 5154 5154
Size: 5278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4392 4576 1305
Size: 3087 184 2007
Fragments arranged by size:
3087 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3456
Fragments arranged by size:
3456 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2114 2233 3236 3595 3754 518
Size: 648 909 39 119 1003 359 159 2042
Fragments arranged by size:
2042 1003 909 648 359 159 119 39
MspA1I CmG'CkG
Cuts at: 1606 2252 2694 3371 4519 4764 1606
Size: 646 442 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 646 442 245
NaeI GCC'GGC
Cuts at: 2257 2257
Size: 5278
NcoI C'CATG_G
Cuts at: 513 1065 2004 2070 2532 513
Size: 552 939 66 462 3259
Fragments arranged by size:
3259 939 552 462 66
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5278
NgoMIV G'CCGG_C
Cuts at: 2255 2255
Size: 5278
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5278
NotI GC'GGCC_GC
Cuts at: 2725 2725
Size: 5278
NspI r_CATG'y
Cuts at: 1132 1229 2636 4181 1132
Size: 97 1407 1545 2229
Fragments arranged by size:
2229 1545 1407 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5278
PciI A'CATG_T
Cuts at: 4177 4177
Size: 5278
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5278
PmeI GTTT'AAAC
Cuts at: 2582 2582
Size: 5278
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3347 3379 4860 4892 3347
Size: 32 1481 32 3733
Fragments arranged by size:
3733 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5278
PsiI TTA'TAA
Cuts at: 2848 2848
Size: 5278
PspOMI G'GGCC_C
Cuts at: 2462 2462
Size: 5278
PstI C_TGCA'G
Cuts at: 830 2630 830
Size: 1800 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5278
PvuII CAG'CTG
Cuts at: 2252 2252
Size: 5278
SalI G'TCGA_C
Cuts at: 1936 2620 1936
Size: 684 4594
SapI GCTCTTCn'nnn_
Cuts at: 2534 2534
Size: 5278
SbfI CC_TGCA'GG
Cuts at: 2630 2630
Size: 5278
ScaI AGT'ACT
Cuts at: 3526 5238 3526
Size: 1712 3566
SfcI C'TryA_G
Cuts at: 826 1045 2626 3761 4442 4633 826
Size: 219 1581 1135 681 191 1471
Fragments arranged by size:
1581 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5278
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5278
SmaI CCC'GGG
Cuts at: 1872 2354 2605 1872
Size: 482 251 4545
Fragments arranged by size:
4545 482 251
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3382 4283 4545 820
Size: 197 926 1439 901 262 1553
Fragments arranged by size:
1553 1439 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3069 493
Size: 2576 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5278
SphI G_CATG'C
Cuts at: 1132 2636 1132
Size: 1504 3774
SrfI GCCC'GGGC
Cuts at: 2354 2354
Size: 5278
SspI AAT'ATT
Cuts at: 5 52 3202 5
Size: 47 3150 2081
Fragments arranged by size:
3150 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5278
StyI C'CwwG_G
Cuts at: 513 1065 2004 2070 2532 2717 513
Size: 552 939 66 462 185 3074
Fragments arranged by size:
3074 939 552 462 185 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 3305 3322 3475 3660 4105 325
Size: 1255 471 1254 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1254 471 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3524 5236 96
Size: 274 80 33 51 1443 1547 1712 138
Fragments arranged by size:
1712 1547 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2144 2176 5238 5270 2144
Size: 32 3062 32 2152
Fragments arranged by size:
3062 2152 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5278
XbaI T'CTAG_A
Cuts at: 2614 2614
Size: 5278
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5278
XmaI C'CCGG_G
Cuts at: 1870 2352 2603 1870
Size: 482 251 4545
Fragments arranged by size:
4545 482 251
XmnI GAAnn'nnTTC
Cuts at: 3407 3407
Size: 5278
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3086 276
Size: 53 83 186 936 1552 2468
Fragments arranged by size:
2468 1552 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SwaI XcmI