Restriction map for
pFN21AB6834
(Circular) (Six-base) MAPSORT of: pFN21AB6834.seq Check: 5285 from: 1 to: 5272
pFN21AB6834 5272 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2122 2122
Size: 5272
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3082 278
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2615 1937
Size: 678 4594
Acc65I G'GTAC_C
Cuts at: 2593 2593
Size: 5272
AclI AA'CG_TT
Cuts at: 1563 3399 3772 1563
Size: 1836 373 3063
Fragments arranged by size:
3063 1836 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3335 4719 4987 1632
Size: 1703 1384 268 1917
Fragments arranged by size:
1917 1703 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5075
AflIII A'CryG_T
Cuts at: 1203 2475 4171 5148 1203
Size: 1272 1696 977 1327
Fragments arranged by size:
1696 1327 1272 977
AgeI A'CCGG_T
Cuts at: 4103 4103
Size: 5272
AhdI GACnn_n'nnGTC
Cuts at: 4001 4001
Size: 5272
AleI CACnn'nnGTG
Cuts at: 1166 2480 1166
Size: 1314 3958
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2579 1184
Size: 1395 3877
AlwNI CAG_nnn'CTG
Cuts at: 3015 4587 3015
Size: 1572 3700
ApaLI G'TGCA_C
Cuts at: 3329 4485 3329
Size: 1156 4116
ApoI r'AATT_y
Cuts at: 1493 2581 2807 2959 5068 1493
Size: 1088 226 152 2109 1697
Fragments arranged by size:
2109 1697 1088 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3826 160
Size: 872 2794 1606
Fragments arranged by size:
2794 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
AvaI C'yCGr_G
Cuts at: 1870 1943 2597 1870
Size: 73 654 4545
Fragments arranged by size:
4545 654 73
BamHI G'GATC_C
Cuts at: 2602 2969 2602
Size: 367 4905
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2593 4048 618
Size: 325 217 627 246 560 1455 1842
Fragments arranged by size:
1842 1455 627 560 325 246 217
BanII G_rGCy'C
Cuts at: 1391 2591 1391
Size: 1200 4072
BbeI G_GCGC'C
Cuts at: 2037 2037
Size: 5272
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3463 3497 1903
Size: 34 1526 34 3678
Fragments arranged by size:
3678 1526 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2167 3164 4374 2167
Size: 997 1210 3065
Fragments arranged by size:
3065 1210 997
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5272
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3883 136
Size: 107 122 71 631 605 2211 1525
Fragments arranged by size:
2211 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5267 5267
Size: 5272
BlpI GC'TnA_GC
Cuts at: 2689 2689
Size: 5272
Bme1580I G_kGCm'C
Cuts at: 1790 2334 3333 4489 1790
Size: 544 999 1156 2573
Fragments arranged by size:
2573 1156 999 544
BmgBI CAC'GTC
Cuts at: 1318 2154 2329 1318
Size: 836 175 4261
Fragments arranged by size:
4261 836 175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3003 3961 442
Size: 416 53 83 399 24 1586 958 1753
Fragments arranged by size:
1753 1586 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3932 1239
Size: 2693 2579
Bpu10I CC'TnA_GC
Cuts at: 2394 2394
Size: 5272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3397 4262 4560 3397
Size: 865 298 4109
Fragments arranged by size:
4109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2426 3935 882
Size: 617 927 1509 2219
Fragments arranged by size:
2219 1509 927 617
BsaAI yAC'GTr
Cuts at: 493 1204 3063 493
Size: 711 1859 2702
Fragments arranged by size:
2702 1859 711
BsaBI GATnn'nnATC
Cuts at: 5266 5266
Size: 5272
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2034 3079 3461 4096
Size: 53 83 186 936 501 1045 382 635
Cuts at: 4096 275
Size: 1451
Fragments arranged by size:
1451 1045 936 635 501 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2124 3705 4103 4377 4524 4935 2124
Size: 1581 398 274 147 411 2461
Fragments arranged by size:
2461 1581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5047
Fragments arranged by size:
5047 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2204 1186
Size: 1018 4254
BseYI C'CCAG_C
Cuts at: 4475 4971 4475
Size: 496 4776
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2352 1163
Size: 573 616 4083
Fragments arranged by size:
4083 616 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2722 3483 4511 1540
Size: 275 186 721 761 1028 2301
Fragments arranged by size:
2301 1028 761 721 275 186
BsiHKAI G_wGCw'C
Cuts at: 2568 3333 3418 4489 2568
Size: 765 85 1071 3351
Fragments arranged by size:
3351 1071 765 85
BsmI GAATG_Cn'
Cuts at: 2783 2876 2783
Size: 93 5179
BsmBI CGTCTCn'nnnn_
Cuts at: 2144 2144
Size: 5272
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2334 2568 2591 3333 3418 4489 1391
Size: 399 544 234 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 399 234 85 23
BspEI T'CCGG_A
Cuts at: 4935 4935
Size: 5272
BspHI T'CATG_A
Cuts at: 3161 4891 3161
Size: 1730 3542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 2627 844
Size: 1278 505 3489
Fragments arranged by size:
3489 1278 505
BsrBI CCG'CTC
Cuts at: 1394 1823 3159 1394
Size: 429 1336 3507
Fragments arranged by size:
3507 1336 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3767 3941 65
Size: 1183 2519 174 1396
Fragments arranged by size:
2519 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 2180 3916 4103 1700
Size: 110 226 144 1736 187 2869
Fragments arranged by size:
2869 1736 226 187 144 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5272
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5272
BssSI C'ACGA_G
Cuts at: 3332 4344 3332
Size: 1012 4260
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2506 2553 794
Size: 1712 47 3513
Fragments arranged by size:
3513 1712 47
BstBI TT'CG_AA
Cuts at: 4076 4076
Size: 5272
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5272
BstXI CCAn_nnnn'nTGG
Cuts at: 2341 2341
Size: 5272
BstYI r'GATC_y
Cuts at: 1972 2490 2602 2969 3354 3371 4823 4909 4921
Size: 518 112 367 385 17 1452 86 12
Cuts at: 4921 5267 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 518 385 367 346 112 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2334 513
Size: 552 1269 3451
Fragments arranged by size:
3451 1269 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4533
Fragments arranged by size:
4533 683 56
BtsI GCAGTG_nn'
Cuts at: 2788 3582 3602 2788
Size: 794 20 4458
Fragments arranged by size:
4458 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5237
DraI TTT'AAA
Cuts at: 2576 2576
Size: 5272
DraIII CAC_nnn'GTG
Cuts at: 2089 2089
Size: 5272
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4279 809
Size: 507 2963 1802
Fragments arranged by size:
2963 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2182 2439 2719
Size: 54 1425 50 275 18 352 257 280
Cuts at: 2719 3608 5237 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 352 280 275 257 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2719 1537
Size: 275 907 4090
Fragments arranged by size:
4090 907 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3202 1353
Size: 1849 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3859 4233 4379 387
Size: 1064 138 2270 374 146 1280
Fragments arranged by size:
2270 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3335 3932 4719 4987 1239
Size: 393 1703 597 787 268 1524
Fragments arranged by size:
1703 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2398 2398
Size: 5272
EcoRI G'AATT_C
Cuts at: 2581 2959 2581
Size: 378 4894
FspI TGC'GCA
Cuts at: 2222 3778 2222
Size: 1556 3716
FspAI rTGC'GCAy
Cuts at: 2222 2222
Size: 5272
HaeII r_GCGC'y
Cuts at: 1678 2037 2202 4419 1678
Size: 359 165 2217 2531
Fragments arranged by size:
2531 2217 359 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3913 3945 3987 4019 1373
Size: 32 135 32 2341 32 42 32 2626
Fragments arranged by size:
2626 2341 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2616 2862 669
Size: 988 281 678 246 3079
Fragments arranged by size:
3079 988 678 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5272
HpaI GTT'AAC
Cuts at: 2862 2862
Size: 5272
KasI G'GCGC_C
Cuts at: 2033 2033
Size: 5272
KpnI G_GTAC'C
Cuts at: 2597 2597
Size: 5272
MfeI C'AATT_G
Cuts at: 2871 2871
Size: 5272
MluI A'CGCG_T
Cuts at: 5148 5148
Size: 5272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2142 4386 4570 1305
Size: 837 2244 184 2007
Fragments arranged by size:
2244 2007 837 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3450
Fragments arranged by size:
3450 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 3230 3589 3748 518
Size: 648 1314 750 359 159 2042
Fragments arranged by size:
2042 1314 750 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2071 2688 3365 4513 4758 1606
Size: 465 617 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 617 465 245
NaeI GCC'GGC
Cuts at: 2038 2182 2038
Size: 144 5128
NarI GG'CG_CC
Cuts at: 2034 2034
Size: 5272
NcoI C'CATG_G
Cuts at: 513 1065 2334 513
Size: 552 1269 3451
Fragments arranged by size:
3451 1269 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5272
NgoMIV G'CCGG_C
Cuts at: 2036 2180 2036
Size: 144 5128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5272
NotI GC'GGCC_GC
Cuts at: 2719 2719
Size: 5272
NspI r_CATG'y
Cuts at: 1132 1229 2221 2630 4175 1132
Size: 97 992 409 1545 2229
Fragments arranged by size:
2229 1545 992 409 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5272
PciI A'CATG_T
Cuts at: 4171 4171
Size: 5272
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2089 1108
Size: 981 4291
PfoI T'CCnGG_A
Cuts at: 2169 2169
Size: 5272
PmeI GTTT'AAAC
Cuts at: 2576 2576
Size: 5272
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 4854 4886 3341
Size: 32 1481 32 3727
Fragments arranged by size:
3727 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2398 2398
Size: 5272
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5272
PsiI TTA'TAA
Cuts at: 2842 2842
Size: 5272
PstI C_TGCA'G
Cuts at: 830 2624 830
Size: 1794 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5272
PvuII CAG'CTG
Cuts at: 2071 2071
Size: 5272
SalI G'TCGA_C
Cuts at: 1936 2614 1936
Size: 678 4594
SbfI CC_TGCA'GG
Cuts at: 2624 2624
Size: 5272
ScaI AGT'ACT
Cuts at: 3520 5232 3520
Size: 1712 3560
SfcI C'TryA_G
Cuts at: 826 1045 2109 2620 3755 4436 4627 826
Size: 219 1064 511 1135 681 191 1471
Fragments arranged by size:
1471 1135 1064 681 511 219 191
SfoI GGC'GCC
Cuts at: 2035 2035
Size: 5272
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
SgrAI Cr'CCGG_yG
Cuts at: 1700 2036 1700
Size: 336 4936
SmaI CCC'GGG
Cuts at: 1872 2599 1872
Size: 727 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3376 4277 4539 820
Size: 197 926 1433 901 262 1553
Fragments arranged by size:
1553 1433 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3063 493
Size: 2570 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5272
SphI G_CATG'C
Cuts at: 1132 2630 1132
Size: 1498 3774
SspI AAT'ATT
Cuts at: 5 52 3196 5
Size: 47 3144 2081
Fragments arranged by size:
3144 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5272
StyI C'CwwG_G
Cuts at: 513 1065 2334 2711 513
Size: 552 1269 377 3074
Fragments arranged by size:
3074 1269 552 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3299 3316 3469 3654 4099 325
Size: 1255 1719 17 153 185 445 1498
Fragments arranged by size:
1719 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 2214 2289
Size: 274 80 33 51 1443 216 21 75
Cuts at: 2289 3518 5230 96
Size: 1229 1712 138
Fragments arranged by size:
1712 1443 1229 274 216 138 80 75
51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2341 2373 5232 5264 2341
Size: 32 2859 32 2349
Fragments arranged by size:
2859 2349 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5272
XbaI T'CTAG_A
Cuts at: 2608 2608
Size: 5272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 2341
Size: 5272
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5272
XmaI C'CCGG_G
Cuts at: 1870 2597 1870
Size: 727 4545
XmnI GAAnn'nnTTC
Cuts at: 3401 3401
Size: 5272
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3080 276
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI