Restriction map for
pFN21AB6809
(Circular) (Six-base) MAPSORT of: pFN21AB6809.seq Check: 436 from: 1 to: 5182
pFN21AB6809 5182 bp
With 182 enzymes: SgfI *
October 20, 2009 10:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2992 278
Size: 53 83 186 936 1456 2468
Fragments arranged by size:
2468 1456 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2322 2525 1937
Size: 385 203 4594
Fragments arranged by size:
4594 385 203
Acc65I G'GTAC_C
Cuts at: 2503 2503
Size: 5182
AclI AA'CG_TT
Cuts at: 1563 3309 3682 1563
Size: 1746 373 3063
Fragments arranged by size:
3063 1746 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 3245 4629 4897 1632
Size: 416 1197 1384 268 1917
Fragments arranged by size:
1917 1384 1197 416 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4985
AflIII A'CryG_T
Cuts at: 1203 2328 4081 5058 1203
Size: 1125 1753 977 1327
Fragments arranged by size:
1753 1327 1125 977
AgeI A'CCGG_T
Cuts at: 4013 4013
Size: 5182
AhdI GACnn_n'nnGTC
Cuts at: 3911 3911
Size: 5182
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5182
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5182
AlwNI CAG_nnn'CTG
Cuts at: 2925 4497 2925
Size: 1572 3610
ApaLI G'TGCA_C
Cuts at: 2213 3239 4395 2213
Size: 1026 1156 3000
Fragments arranged by size:
3000 1156 1026
ApoI r'AATT_y
Cuts at: 1493 2491 2717 2869 4978 1493
Size: 998 226 152 2109 1697
Fragments arranged by size:
2109 1697 998 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3736 160
Size: 872 2704 1606
Fragments arranged by size:
2704 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5182
AvaI C'yCGr_G
Cuts at: 1870 1943 2472 2507 1870
Size: 73 529 35 4545
Fragments arranged by size:
4545 529 73 35
AvrII C'CTAG_G
Cuts at: 2041 2041
Size: 5182
BamHI G'GATC_C
Cuts at: 2512 2879 2512
Size: 367 4815
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2425 2503 3958 618
Size: 325 217 627 638 78 1455 1842
Fragments arranged by size:
1842 1455 638 627 325 217 78
BanII G_rGCy'C
Cuts at: 1391 2355 2501 1391
Size: 964 146 4072
Fragments arranged by size:
4072 964 146
BbeI G_GCGC'C
Cuts at: 2429 2429
Size: 5182
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2373 928
Size: 971 474 3737
Fragments arranged by size:
3737 971 474
BbvCI CC'TCA_GC
Cuts at: 2285 2285
Size: 5182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3373 3407 1903
Size: 34 1436 34 3678
Fragments arranged by size:
3678 1436 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3074 4284 3074
Size: 1210 3972
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5182
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2265 3793 136
Size: 107 122 71 631 605 593 1528 1525
Fragments arranged by size:
1528 1525 631 605 593 122 107 71
BglII A'GATC_T
Cuts at: 5177 5177
Size: 5182
BlpI GC'TnA_GC
Cuts at: 2142 2599 2142
Size: 457 4725
Bme1580I G_kGCm'C
Cuts at: 1790 2217 3243 4399 1790
Size: 427 1026 1156 2573
Fragments arranged by size:
2573 1156 1026 427
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5182
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2913 3871 442
Size: 416 53 83 399 24 1496 958 1753
Fragments arranged by size:
1753 1496 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3842 1239
Size: 2603 2579
Bpu10I CC'TnA_GC
Cuts at: 2285 2285
Size: 5182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2282 3307 4172 4470 2282
Size: 1025 865 298 2994
Fragments arranged by size:
2994 1025 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3845 882
Size: 617 2346 2219
Fragments arranged by size:
2346 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2973 493
Size: 711 1769 2702
Fragments arranged by size:
2702 1769 711
BsaBI GATnn'nnATC
Cuts at: 5176 5176
Size: 5182
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2160 2426 2989 3371
Size: 53 83 186 936 627 266 563 382
Cuts at: 3371 4006 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 627 563 382 266 186
83 53
BsaWI w'CCGG_w
Cuts at: 3615 4013 4287 4434 4845 3615
Size: 398 274 147 411 3952
Fragments arranged by size:
3952 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4957
Fragments arranged by size:
4957 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5182
BseYI C'CCAG_C
Cuts at: 4385 4881 4385
Size: 496 4686
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4609
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2632 3393 4421 1540
Size: 275 186 631 761 1028 2301
Fragments arranged by size:
2301 1028 761 631 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 2355 3243 3328 4399 2217
Size: 138 888 85 1071 3000
Fragments arranged by size:
3000 1071 888 138 85
BsmI GAATG_Cn'
Cuts at: 2693 2786 2693
Size: 93 5089
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2355 2501 3243 3328 4399 1391
Size: 399 427 138 146 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 399 146 138 85
BspEI T'CCGG_A
Cuts at: 4845 4845
Size: 5182
BspHI T'CATG_A
Cuts at: 3071 4801 3071
Size: 1730 3452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2537 844
Size: 1693 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2305 3069 1394
Size: 429 482 764 3507
Fragments arranged by size:
3507 764 482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3677 3851 65
Size: 1183 2429 174 1396
Fragments arranged by size:
2429 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3826 4013 1700
Size: 110 2016 187 2869
Fragments arranged by size:
2869 2016 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5182
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5182
BssSI C'ACGA_G
Cuts at: 3242 4254 3242
Size: 1012 4170
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5182
BstBI TT'CG_AA
Cuts at: 3986 3986
Size: 5182
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5182
BstXI CCAn_nnnn'nTGG
Cuts at: 2203 2203
Size: 5182
BstYI r'GATC_y
Cuts at: 1972 2434 2512 2879 3264 3281 4733 4819 4831
Size: 462 78 367 385 17 1452 86 12
Cuts at: 4831 5177 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 462 385 367 346 86 78
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2234 513
Size: 552 939 230 3461
Fragments arranged by size:
3461 939 552 230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4443
Fragments arranged by size:
4443 683 56
BtsI GCAGTG_nn'
Cuts at: 2698 3492 3512 2698
Size: 794 20 4368
Fragments arranged by size:
4368 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5182
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5147
DraI TTT'AAA
Cuts at: 2486 2486
Size: 5182
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4189 809
Size: 507 2873 1802
Fragments arranged by size:
2873 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 2277 2629
Size: 54 1425 50 275 18 360 87 352
Cuts at: 2629 3518 5147 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 360 352 275 87 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2629 1537
Size: 275 817 4090
Fragments arranged by size:
4090 817 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3112 1353
Size: 1759 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3769 4143 4289 387
Size: 1064 138 2180 374 146 1280
Fragments arranged by size:
2180 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2353 2353
Size: 5182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 3245 3842 4629 4897 1239
Size: 393 416 1197 597 787 268 1524
Fragments arranged by size:
1524 1197 787 597 416 393 268
EcoRI G'AATT_C
Cuts at: 2491 2869 2491
Size: 378 4804
FspI TGC'GCA
Cuts at: 3688 3688
Size: 5182
HaeII r_GCGC'y
Cuts at: 1678 2429 4329 1678
Size: 751 1900 2531
Fragments arranged by size:
2531 1900 751
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3823 3855 3897 3929 1373
Size: 32 135 32 2251 32 42 32 2626
Fragments arranged by size:
2626 2251 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2526 2772 669
Size: 988 281 588 246 3079
Fragments arranged by size:
3079 988 588 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5182
HpaI GTT'AAC
Cuts at: 2772 2772
Size: 5182
KasI G'GCGC_C
Cuts at: 2425 2425
Size: 5182
KpnI G_GTAC'C
Cuts at: 2507 2507
Size: 5182
MfeI C'AATT_G
Cuts at: 2781 2781
Size: 5182
MluI A'CGCG_T
Cuts at: 5058 5058
Size: 5182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4296 4480 1305
Size: 2991 184 2007
Fragments arranged by size:
2991 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 2279 10
Size: 54 1425 343 360 87 2913
Fragments arranged by size:
2913 1425 360 343 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 3140 3499 3658 518
Size: 648 1002 972 359 159 2042
Fragments arranged by size:
2042 1002 972 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 2236 2598 3275 4423 4668 1606
Size: 540 90 362 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 540 362 245 90
NarI GG'CG_CC
Cuts at: 2426 2426
Size: 5182
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3691
Fragments arranged by size:
3691 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5182
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5182
NotI GC'GGCC_GC
Cuts at: 2629 2629
Size: 5182
NspI r_CATG'y
Cuts at: 1132 1229 2173 2332 2540 4085 1132
Size: 97 944 159 208 1545 2229
Fragments arranged by size:
2229 1545 944 208 159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5182
PciI A'CATG_T
Cuts at: 2328 4081 2328
Size: 1753 3429
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5182
PfoI T'CCnGG_A
Cuts at: 2202 2202
Size: 5182
PmeI GTTT'AAAC
Cuts at: 2486 2486
Size: 5182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 4764 4796 3251
Size: 32 1481 32 3637
Fragments arranged by size:
3637 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5182
PsiI TTA'TAA
Cuts at: 2752 2752
Size: 5182
PstI C_TGCA'G
Cuts at: 830 2534 830
Size: 1704 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5182
SacI G_AGCT'C
Cuts at: 2355 2355
Size: 5182
SacII CC_GC'GG
Cuts at: 2237 2237
Size: 5182
SalI G'TCGA_C
Cuts at: 1936 2524 1936
Size: 588 4594
SbfI CC_TGCA'GG
Cuts at: 2534 2534
Size: 5182
ScaI AGT'ACT
Cuts at: 3430 5142 3430
Size: 1712 3470
SfcI C'TryA_G
Cuts at: 826 1045 2530 3665 4346 4537 826
Size: 219 1485 1135 681 191 1471
Fragments arranged by size:
1485 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2427 2427
Size: 5182
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5182
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5182
SmaI CCC'GGG
Cuts at: 1872 2509 1872
Size: 637 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2297 3286 4187 4449 820
Size: 197 926 354 989 901 262 1553
Fragments arranged by size:
1553 989 926 901 354 262 197
SnaBI TAC'GTA
Cuts at: 493 2973 493
Size: 2480 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5182
SphI G_CATG'C
Cuts at: 1132 2540 1132
Size: 1408 3774
SspI AAT'ATT
Cuts at: 5 52 3106 5
Size: 47 3054 2081
Fragments arranged by size:
3054 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5182
StyI C'CwwG_G
Cuts at: 513 1065 2004 2041 2121 2374 2621 513
Size: 552 939 37 80 253 247 3074
Fragments arranged by size:
3074 939 552 253 247 80 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 3209 3226 3379 3564 4009 325
Size: 1255 588 1041 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1041 588 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3428 5140 96
Size: 274 80 33 51 1443 1451 1712 138
Fragments arranged by size:
1712 1451 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5142 5174 5142
Size: 32 5150
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5182
XbaI T'CTAG_A
Cuts at: 2518 2518
Size: 5182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2119 2200 2119
Size: 81 5101
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5182
XmaI C'CCGG_G
Cuts at: 1870 2507 1870
Size: 637 4545
XmnI GAAnn'nnTTC
Cuts at: 3311 3311
Size: 5182
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2990 276
Size: 53 83 186 936 1456 2468
Fragments arranged by size:
2468 1456 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI
SapI SexAI SfiI SrfI SwaI