Restriction map for
pFN21AB6795
(Circular) (Six-base) MAPSORT of: pFN21AB6795.seq Check: 393 from: 1 to: 5089
pFN21AB6795 5089 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2183 2183
Size: 5089
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2899 278
Size: 53 83 186 936 1363 2468
Fragments arranged by size:
2468 1363 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2012 2432 1937
Size: 75 420 4594
Fragments arranged by size:
4594 420 75
Acc65I G'GTAC_C
Cuts at: 2410 2410
Size: 5089
AclI AA'CG_TT
Cuts at: 1563 3216 3589 1563
Size: 1653 373 3063
Fragments arranged by size:
3063 1653 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3152 4536 4804 1632
Size: 1520 1384 268 1917
Fragments arranged by size:
1917 1520 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4892
AflIII A'CryG_T
Cuts at: 1203 2253 3988 4965 1203
Size: 1050 1735 977 1327
Fragments arranged by size:
1735 1327 1050 977
AgeI A'CCGG_T
Cuts at: 3920 3920
Size: 5089
AhdI GACnn_n'nnGTC
Cuts at: 3818 3818
Size: 5089
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5089
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5089
AlwNI CAG_nnn'CTG
Cuts at: 2114 2832 4404 2114
Size: 718 1572 2799
Fragments arranged by size:
2799 1572 718
ApaLI G'TGCA_C
Cuts at: 3146 4302 3146
Size: 1156 3933
ApoI r'AATT_y
Cuts at: 1493 2398 2624 2776 4885 1493
Size: 905 226 152 2109 1697
Fragments arranged by size:
2109 1697 905 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3643 160
Size: 872 2611 1606
Fragments arranged by size:
2611 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5089
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2414 1870
Size: 73 108 363 4545
Fragments arranged by size:
4545 363 108 73
BamHI G'GATC_C
Cuts at: 2419 2786 2419
Size: 367 4722
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2410 3865 618
Size: 325 217 627 623 1455 1842
Fragments arranged by size:
1842 1455 627 623 325 217
BanII G_rGCy'C
Cuts at: 1391 2408 1391
Size: 1017 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3280 3314 1903
Size: 34 1343 34 3678
Fragments arranged by size:
3678 1343 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2981 4191 2981
Size: 1210 3879
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5089
BfrBI ATG'CAT
Cuts at: 2092 2092
Size: 5089
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3700 136
Size: 107 122 71 631 605 2028 1525
Fragments arranged by size:
2028 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5084 5084
Size: 5089
BlpI GC'TnA_GC
Cuts at: 2506 2506
Size: 5089
Bme1580I G_kGCm'C
Cuts at: 1790 2273 2300 3150 4306 1790
Size: 483 27 850 1156 2573
Fragments arranged by size:
2573 1156 850 483 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5089
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2820 3778 442
Size: 416 53 83 399 24 1403 958 1753
Fragments arranged by size:
1753 1403 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3749 1239
Size: 2510 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3214 4079 4377 3214
Size: 865 298 3926
Fragments arranged by size:
3926 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2147 2331 3752 882
Size: 617 648 184 1421 2219
Fragments arranged by size:
2219 1421 648 617 184
BsaAI yAC'GTr
Cuts at: 493 1204 2274 2880 493
Size: 711 1070 606 2702
Fragments arranged by size:
2702 1070 711 606
BsaBI GATnn'nnATC
Cuts at: 5083 5083
Size: 5089
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2896 3278 3913 275
Size: 53 83 186 936 1363 382 635 1451
Fragments arranged by size:
1451 1363 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3522 3920 4194 4341 4752 3522
Size: 398 274 147 411 3859
Fragments arranged by size:
3859 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4864
Fragments arranged by size:
4864 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 1186
Size: 1036 4053
BseYI C'CCAG_C
Cuts at: 4292 4788 4292
Size: 496 4593
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2337 1163
Size: 573 601 3915
Fragments arranged by size:
3915 601 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2539 3300 4328 1540
Size: 275 186 538 761 1028 2301
Fragments arranged by size:
2301 1028 761 538 275 186
BsiHKAI G_wGCw'C
Cuts at: 3150 3235 4306 3150
Size: 85 1071 3933
Fragments arranged by size:
3933 1071 85
BsmI GAATG_Cn'
Cuts at: 2600 2693 2600
Size: 93 4996
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2273 2300 2408 3150 3235 4306 1391
Size: 399 483 27 108 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 483 399 108 85 27
BspEI T'CCGG_A
Cuts at: 4752 4752
Size: 5089
BspHI T'CATG_A
Cuts at: 2978 4708 2978
Size: 1730 3359
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2183 2444 844
Size: 1339 261 3489
Fragments arranged by size:
3489 1339 261
BsrBI CCG'CTC
Cuts at: 1394 1823 2976 1394
Size: 429 1153 3507
Fragments arranged by size:
3507 1153 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2103 2187 3584 3758 65
Size: 1183 855 84 1397 174 1396
Fragments arranged by size:
1397 1396 1183 855 174 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 2259 3733 3920 1700
Size: 110 449 1474 187 2869
Fragments arranged by size:
2869 1474 449 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5089
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5089
BssSI C'ACGA_G
Cuts at: 3149 4161 3149
Size: 1012 4077
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5089
BstBI TT'CG_AA
Cuts at: 3893 3893
Size: 5089
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5089
BstXI CCAn_nnnn'nTGG
Cuts at: 2173 2357 2173
Size: 184 4905
BstYI r'GATC_y
Cuts at: 1972 2419 2786 3171 3188 4640 4726 4738 5084
Size: 447 367 385 17 1452 86 12 346
Cuts at: 5084 1972
Size: 1977
Fragments arranged by size:
1977 1452 447 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4350
Fragments arranged by size:
4350 683 56
BtsI GCAGTG_nn'
Cuts at: 2605 3399 3419 2605
Size: 794 20 4275
Fragments arranged by size:
4275 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5089
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5054
DraI TTT'AAA
Cuts at: 2393 2393
Size: 5089
DraIII CAC_nnn'GTG
Cuts at: 2037 2037
Size: 5089
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2183 4096 809
Size: 507 867 1913 1802
Fragments arranged by size:
1913 1802 867 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2135 2211 2536
Size: 54 1425 50 275 18 305 76 325
Cuts at: 2536 3425 5054 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 325 305 275 76 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2536 1537
Size: 275 724 4090
Fragments arranged by size:
4090 724 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 3019 1353
Size: 887 779 3423
Fragments arranged by size:
3423 887 779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3676 4050 4196 387
Size: 1064 138 2087 374 146 1280
Fragments arranged by size:
2087 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3152 3749 4536 4804 1239
Size: 393 1520 597 787 268 1524
Fragments arranged by size:
1524 1520 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2118 2118
Size: 5089
EcoRI G'AATT_C
Cuts at: 2398 2776 2398
Size: 378 4711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 5089
FspI TGC'GCA
Cuts at: 3595 3595
Size: 5089
HaeII r_GCGC'y
Cuts at: 1678 4236 1678
Size: 2558 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3730 3762 3804 3836 1373
Size: 32 135 32 2158 32 42 32 2626
Fragments arranged by size:
2626 2158 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2013 2433 2679 669
Size: 988 281 75 420 246 3079
Fragments arranged by size:
3079 988 420 281 246 75
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5089
HpaI GTT'AAC
Cuts at: 2679 2679
Size: 5089
KpnI G_GTAC'C
Cuts at: 2414 2414
Size: 5089
MfeI C'AATT_G
Cuts at: 2688 2688
Size: 5089
MluI A'CGCG_T
Cuts at: 4965 4965
Size: 5089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4203 4387 1305
Size: 2898 184 2007
Fragments arranged by size:
2898 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 10
Size: 54 1425 343 381 2886
Fragments arranged by size:
2886 1425 381 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2171 2283 3047 3406 3565 518
Size: 648 921 84 112 764 359 159 2042
Fragments arranged by size:
2042 921 764 648 359 159 112 84
MspA1I CmG'CkG
Cuts at: 1606 2505 3182 4330 4575 1606
Size: 899 677 1148 245 2120
Fragments arranged by size:
2120 1148 899 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4537
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5089
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5089
NotI GC'GGCC_GC
Cuts at: 2536 2536
Size: 5089
NsiI A_TGCA'T
Cuts at: 2094 2094
Size: 5089
NspI r_CATG'y
Cuts at: 1132 1229 2257 2447 3992 1132
Size: 97 1028 190 1545 2229
Fragments arranged by size:
2229 1545 1028 190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5089
PciI A'CATG_T
Cuts at: 2253 3988 2253
Size: 1735 3354
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5089
PmeI GTTT'AAAC
Cuts at: 2393 2393
Size: 5089
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 4671 4703 3158
Size: 32 1481 32 3544
Fragments arranged by size:
3544 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5089
PsiI TTA'TAA
Cuts at: 2659 2659
Size: 5089
PspXI vC'TCGA_Gb
Cuts at: 2051 2051
Size: 5089
PstI C_TGCA'G
Cuts at: 830 2334 2441 830
Size: 1504 107 3478
Fragments arranged by size:
3478 1504 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5089
SalI G'TCGA_C
Cuts at: 1936 2011 2431 1936
Size: 75 420 4594
Fragments arranged by size:
4594 420 75
SbfI CC_TGCA'GG
Cuts at: 2334 2441 2334
Size: 107 4982
ScaI AGT'ACT
Cuts at: 2381 3337 5049 2381
Size: 956 1712 2421
Fragments arranged by size:
2421 1712 956
SfcI C'TryA_G
Cuts at: 826 1045 2330 2437 3572 4253 4444 826
Size: 219 1285 107 1135 681 191 1471
Fragments arranged by size:
1471 1285 1135 681 219 191 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5089
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5089
SmaI CCC'GGG
Cuts at: 1872 2416 1872
Size: 544 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2051 3193 4094 4356 820
Size: 197 926 108 1142 901 262 1553
Fragments arranged by size:
1553 1142 926 901 262 197 108
SnaBI TAC'GTA
Cuts at: 493 2880 493
Size: 2387 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5089
SphI G_CATG'C
Cuts at: 1132 2447 1132
Size: 1315 3774
SspI AAT'ATT
Cuts at: 5 52 3013 5
Size: 47 2961 2081
Fragments arranged by size:
2961 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5089
StyI C'CwwG_G
Cuts at: 513 1065 2292 2528 513
Size: 552 1227 236 3074
Fragments arranged by size:
3074 1227 552 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3116 3133 3286 3471 3916 325
Size: 1255 1536 17 153 185 445 1498
Fragments arranged by size:
1536 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2379 3335 5047
Size: 274 80 33 51 1443 402 956 1712
Cuts at: 5047 96
Size: 138
Fragments arranged by size:
1712 1443 956 402 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5049 5081 5049
Size: 32 5057
Tth111I GACn'n_nGTC
Cuts at: 1316 2180 1316
Size: 864 4225
XbaI T'CTAG_A
Cuts at: 2425 2425
Size: 5089
XhoI C'TCGA_G
Cuts at: 1943 2051 1943
Size: 108 4981
XmaI C'CCGG_G
Cuts at: 1870 2414 1870
Size: 544 4545
XmnI GAAnn'nnTTC
Cuts at: 3218 3218
Size: 5089
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2897 276
Size: 53 83 186 936 1363 2468
Fragments arranged by size:
2468 1363 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI