Restriction map for
pFN21AB6743
(Circular) (Six-base) MAPSORT of: pFN21AB6743.seq Check: 4566 from: 1 to: 5002
pFN21AB6743 5002 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2111 2111
Size: 5002
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2812 278
Size: 53 83 186 936 1276 2468
Fragments arranged by size:
2468 1276 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2345 1937
Size: 408 4594
Acc65I G'GTAC_C
Cuts at: 2323 2323
Size: 5002
AclI AA'CG_TT
Cuts at: 1563 3129 3502 1563
Size: 1566 373 3063
Fragments arranged by size:
3063 1566 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3065 4449 4717 1632
Size: 1433 1384 268 1917
Fragments arranged by size:
1917 1433 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4805
AflIII A'CryG_T
Cuts at: 1203 3901 4878 1203
Size: 2698 977 1327
Fragments arranged by size:
2698 1327 977
AgeI A'CCGG_T
Cuts at: 3833 3833
Size: 5002
AhdI GACnn_n'nnGTC
Cuts at: 3731 3731
Size: 5002
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5002
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5002
AlwNI CAG_nnn'CTG
Cuts at: 2745 4317 2745
Size: 1572 3430
ApaLI G'TGCA_C
Cuts at: 3059 4215 3059
Size: 1156 3846
ApoI r'AATT_y
Cuts at: 1493 2311 2537 2689 4798 1493
Size: 818 226 152 2109 1697
Fragments arranged by size:
2109 1697 818 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3556 160
Size: 872 2524 1606
Fragments arranged by size:
2524 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5002
AvaI C'yCGr_G
Cuts at: 1870 1943 2327 1870
Size: 73 384 4545
Fragments arranged by size:
4545 384 73
BamHI G'GATC_C
Cuts at: 2332 2699 2332
Size: 367 4635
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2323 3778 618
Size: 325 217 627 536 1455 1842
Fragments arranged by size:
1842 1455 627 536 325 217
BanII G_rGCy'C
Cuts at: 1391 2070 2321 1391
Size: 679 251 4072
Fragments arranged by size:
4072 679 251
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2303 928
Size: 971 404 3627
Fragments arranged by size:
3627 971 404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3193 3227 1903
Size: 34 1256 34 3678
Fragments arranged by size:
3678 1256 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2894 4104 2894
Size: 1210 3792
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5002
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3613 136
Size: 107 122 71 631 605 1941 1525
Fragments arranged by size:
1941 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2130 2223 4997 2130
Size: 93 2774 2135
Fragments arranged by size:
2774 2135 93
BlpI GC'TnA_GC
Cuts at: 2073 2419 2073
Size: 346 4656
Bme1580I G_kGCm'C
Cuts at: 1790 3063 4219 1790
Size: 1273 1156 2573
Fragments arranged by size:
2573 1273 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5002
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2090 2733 3691
Size: 416 53 83 399 24 673 643 958
Cuts at: 3691 442
Size: 1753
Fragments arranged by size:
1753 958 673 643 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3662 1239
Size: 2423 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3127 3992 4290 3127
Size: 865 298 3839
Fragments arranged by size:
3839 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3665 882
Size: 617 2166 2219
Fragments arranged by size:
2219 2166 617
BsaAI yAC'GTr
Cuts at: 493 1204 2793 493
Size: 711 1589 2702
Fragments arranged by size:
2702 1589 711
BsaBI GATnn'nnATC
Cuts at: 4996 4996
Size: 5002
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2809 3191 3826 275
Size: 53 83 186 936 1276 382 635 1451
Fragments arranged by size:
1451 1276 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3435 3833 4107 4254 4665 3435
Size: 398 274 147 411 3772
Fragments arranged by size:
3772 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2275 2305 1178
Size: 30 165 30 872 30 3875
Fragments arranged by size:
3875 872 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5002
BseYI C'CCAG_C
Cuts at: 4205 4701 4205
Size: 496 4506
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2159 1163
Size: 573 423 4006
Fragments arranged by size:
4006 573 423
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2452 3213 4241 1540
Size: 275 186 451 761 1028 2301
Fragments arranged by size:
2301 1028 761 451 275 186
BsiHKAI G_wGCw'C
Cuts at: 3063 3148 4219 3063
Size: 85 1071 3846
Fragments arranged by size:
3846 1071 85
BsmI GAATG_Cn'
Cuts at: 2513 2606 2513
Size: 93 4909
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2321 3063 3148 4219 1391
Size: 399 280 251 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 280 251 85
BspEI T'CCGG_A
Cuts at: 4665 4665
Size: 5002
BspHI T'CATG_A
Cuts at: 2891 4621 2891
Size: 1730 3272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2111 2357 844
Size: 1267 246 3489
Fragments arranged by size:
3489 1267 246
BsrBI CCG'CTC
Cuts at: 1394 1823 2889 1394
Size: 429 1066 3507
Fragments arranged by size:
3507 1066 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3497 3671 65
Size: 1183 2249 174 1396
Fragments arranged by size:
2249 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3646 3833 1700
Size: 110 1836 187 2869
Fragments arranged by size:
2869 1836 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5002
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5002
BssSI C'ACGA_G
Cuts at: 3062 4074 3062
Size: 1012 3990
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2090 794
Size: 1296 3706
BstBI TT'CG_AA
Cuts at: 3806 3806
Size: 5002
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5002
BstYI r'GATC_y
Cuts at: 1972 2130 2223 2332 2699 3084 3101 4553 4639
Size: 158 93 109 367 385 17 1452 86
Cuts at: 4639 4651 4997 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 158 109 93
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2262 513
Size: 552 1197 3253
Fragments arranged by size:
3253 1197 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4263
Fragments arranged by size:
4263 683 56
BtsI GCAGTG_nn'
Cuts at: 2518 3312 3332 2518
Size: 794 20 4188
Fragments arranged by size:
4188 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5002
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4967
DraI TTT'AAA
Cuts at: 2306 2306
Size: 5002
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4009 809
Size: 507 2693 1802
Fragments arranged by size:
2693 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2449 3338
Size: 54 1425 50 275 18 384 235 889
Cuts at: 3338 4967 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 384 275 235 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2449 1537
Size: 275 637 4090
Fragments arranged by size:
4090 637 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2932 1353
Size: 1579 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3589 3963 4109 387
Size: 1064 138 2000 374 146 1280
Fragments arranged by size:
2000 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3065 3662 4449 4717 1239
Size: 393 1433 597 787 268 1524
Fragments arranged by size:
1524 1433 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2311 2689 2311
Size: 378 4624
FspI TGC'GCA
Cuts at: 3508 3508
Size: 5002
HaeII r_GCGC'y
Cuts at: 1678 4149 1678
Size: 2471 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3643 3675 3717 3749 1373
Size: 32 135 32 2071 32 42 32 2626
Fragments arranged by size:
2626 2071 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2346 2592 669
Size: 988 281 408 246 3079
Fragments arranged by size:
3079 988 408 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5002
HpaI GTT'AAC
Cuts at: 2592 2592
Size: 5002
KpnI G_GTAC'C
Cuts at: 2327 2327
Size: 5002
MfeI C'AATT_G
Cuts at: 2601 2601
Size: 5002
MluI A'CGCG_T
Cuts at: 4878 4878
Size: 5002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4116 4300 1305
Size: 2811 184 2007
Fragments arranged by size:
2811 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 10
Size: 54 1425 343 384 2796
Fragments arranged by size:
2796 1425 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2960 3319 3478 518
Size: 648 1794 359 159 2042
Fragments arranged by size:
2042 1794 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2418 3095 4243 4488 1606
Size: 812 677 1148 245 2120
Fragments arranged by size:
2120 1148 812 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2262 513
Size: 552 1197 3253
Fragments arranged by size:
3253 1197 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5002
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5002
NotI GC'GGCC_GC
Cuts at: 2449 2449
Size: 5002
NruI TCG'CGA
Cuts at: 2150 2150
Size: 5002
NspI r_CATG'y
Cuts at: 1132 1229 2360 3905 1132
Size: 97 1131 1545 2229
Fragments arranged by size:
2229 1545 1131 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5002
PciI A'CATG_T
Cuts at: 3901 3901
Size: 5002
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5002
PmeI GTTT'AAAC
Cuts at: 2306 2306
Size: 5002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3071 3103 4584 4616 3071
Size: 32 1481 32 3457
Fragments arranged by size:
3457 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5002
PsiI TTA'TAA
Cuts at: 2112 2572 2112
Size: 460 4542
PstI C_TGCA'G
Cuts at: 830 2354 830
Size: 1524 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5002
SalI G'TCGA_C
Cuts at: 1936 2344 1936
Size: 408 4594
SbfI CC_TGCA'GG
Cuts at: 2354 2354
Size: 5002
ScaI AGT'ACT
Cuts at: 3250 4962 3250
Size: 1712 3290
SfcI C'TryA_G
Cuts at: 826 1045 2155 2350 3485 4166 4357 826
Size: 219 1110 195 1135 681 191 1471
Fragments arranged by size:
1471 1135 1110 681 219 195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5002
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5002
SmaI CCC'GGG
Cuts at: 1872 2329 1872
Size: 457 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3106 4007 4269 820
Size: 197 926 1163 901 262 1553
Fragments arranged by size:
1553 1163 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2793 493
Size: 2300 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5002
SphI G_CATG'C
Cuts at: 1132 2360 1132
Size: 1228 3774
SspI AAT'ATT
Cuts at: 5 52 2926 5
Size: 47 2874 2081
Fragments arranged by size:
2874 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5002
StyI C'CwwG_G
Cuts at: 513 1065 2062 2217 2262 2441 513
Size: 552 997 155 45 179 3074
Fragments arranged by size:
3074 997 552 179 155 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3029 3046 3199 3384 3829 325
Size: 1255 1449 17 153 185 445 1498
Fragments arranged by size:
1498 1449 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3248 4960 96
Size: 274 80 33 51 1443 1271 1712 138
Fragments arranged by size:
1712 1443 1271 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2041 2073 4962 4994 2041
Size: 32 2889 32 2049
Fragments arranged by size:
2889 2049 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5002
XbaI T'CTAG_A
Cuts at: 2338 2338
Size: 5002
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2269 2269
Size: 5002
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5002
XmaI C'CCGG_G
Cuts at: 1870 2327 1870
Size: 457 4545
XmnI GAAnn'nnTTC
Cuts at: 3131 3131
Size: 5002
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2810 276
Size: 53 83 186 936 1276 2468
Fragments arranged by size:
2468 1276 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI