Restriction map for
pFN21AB6724
(Circular) (Six-base) MAPSORT of: pFN21AB6724.seq Check: 6890 from: 1 to: 5110
pFN21AB6724 5110 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2082 2920 278
Size: 53 83 186 936 546 838 2468
Fragments arranged by size:
2468 936 838 546 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2013 2453 1937
Size: 76 440 4594
Fragments arranged by size:
4594 440 76
Acc65I G'GTAC_C
Cuts at: 2431 2431
Size: 5110
AclI AA'CG_TT
Cuts at: 1563 3237 3610 1563
Size: 1674 373 3063
Fragments arranged by size:
3063 1674 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 3173 4557 4825 1632
Size: 503 1038 1384 268 1917
Fragments arranged by size:
1917 1384 1038 503 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4913
AflIII A'CryG_T
Cuts at: 1203 4009 4986 1203
Size: 2806 977 1327
Fragments arranged by size:
2806 1327 977
AgeI A'CCGG_T
Cuts at: 3941 3941
Size: 5110
AhdI GACnn_n'nnGTC
Cuts at: 3839 3839
Size: 5110
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5110
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5110
AlwNI CAG_nnn'CTG
Cuts at: 2853 4425 2853
Size: 1572 3538
ApaLI G'TGCA_C
Cuts at: 3167 4323 3167
Size: 1156 3954
ApoI r'AATT_y
Cuts at: 1493 2050 2419 2645 2797 4906 1493
Size: 557 369 226 152 2109 1697
Fragments arranged by size:
2109 1697 557 369 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3664 160
Size: 872 2632 1606
Fragments arranged by size:
2632 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5110
AvaI C'yCGr_G
Cuts at: 1870 1943 2435 1870
Size: 73 492 4545
Fragments arranged by size:
4545 492 73
BamHI G'GATC_C
Cuts at: 2440 2807 2440
Size: 367 4743
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2431 3886 618
Size: 325 217 627 644 1455 1842
Fragments arranged by size:
1842 1455 644 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2429 1391
Size: 1038 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3301 3335 1903
Size: 34 50 34 1280 34 3678
Fragments arranged by size:
3678 1280 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3002 4212 3002
Size: 1210 3900
BclI T'GATC_A
Cuts at: 1548 2154 1548
Size: 606 4504
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3721 136
Size: 107 122 71 631 605 2049 1525
Fragments arranged by size:
2049 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5105 5105
Size: 5110
BlpI GC'TnA_GC
Cuts at: 2168 2527 2168
Size: 359 4751
Bme1580I G_kGCm'C
Cuts at: 1790 3171 4327 1790
Size: 1381 1156 2573
Fragments arranged by size:
2573 1381 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2031 2323 2841
Size: 416 53 83 399 24 614 292 518
Cuts at: 2841 3799 442
Size: 958 1753
Fragments arranged by size:
1753 958 614 518 416 399 292 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5110
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2300 2300
Size: 5110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2378 3770 1239
Size: 1031 108 1392 2579
Fragments arranged by size:
2579 1392 1031 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3235 4100 4398 3235
Size: 865 298 3947
Fragments arranged by size:
3947 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3773 882
Size: 617 2274 2219
Fragments arranged by size:
2274 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2901 493
Size: 711 1697 2702
Fragments arranged by size:
2702 1697 711
BsaBI GATnn'nnATC
Cuts at: 5104 5104
Size: 5110
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 2917 3299 3934
Size: 53 83 186 936 546 838 382 635
Cuts at: 3934 275
Size: 1451
Fragments arranged by size:
1451 936 838 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3543 3941 4215 4362 4773 3543
Size: 398 274 147 411 3880
Fragments arranged by size:
3880 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4885
Fragments arranged by size:
4885 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2234 1186
Size: 886 162 4062
Fragments arranged by size:
4062 886 162
BseYI C'CCAG_C
Cuts at: 2268 4313 4809 2268
Size: 2045 496 2569
Fragments arranged by size:
2569 2045 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 2315 2336 2426 1163
Size: 573 420 159 21 90 3847
Fragments arranged by size:
3847 573 420 159 90 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2560 3321 4349 1540
Size: 275 186 559 761 1028 2301
Fragments arranged by size:
2301 1028 761 559 275 186
BsiHKAI G_wGCw'C
Cuts at: 2174 3171 3256 4327 2174
Size: 997 85 1071 2957
Fragments arranged by size:
2957 1071 997 85
BsmI GAATG_Cn'
Cuts at: 2621 2714 2621
Size: 93 5017
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2174 2429 3171 3256 4327 1391
Size: 399 384 255 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 384 255 85
BspEI T'CCGG_A
Cuts at: 4773 4773
Size: 5110
BspHI T'CATG_A
Cuts at: 2999 4729 2999
Size: 1730 3380
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2398 2465 844
Size: 1229 325 67 3489
Fragments arranged by size:
3489 1229 325 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2997 1394
Size: 429 1174 3507
Fragments arranged by size:
3507 1174 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3605 3779 65
Size: 1183 2357 174 1396
Fragments arranged by size:
2357 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3754 3941 1700
Size: 110 1944 187 2869
Fragments arranged by size:
2869 1944 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5110
BssHII G'CGCG_C
Cuts at: 1925 2068 1925
Size: 143 4967
BssSI C'ACGA_G
Cuts at: 3170 4182 3170
Size: 1012 4098
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5110
BstBI TT'CG_AA
Cuts at: 3914 3914
Size: 5110
BstEII G'GTnAC_C
Cuts at: 1184 2144 1184
Size: 960 4150
BstYI r'GATC_y
Cuts at: 1972 2224 2440 2807 3192 3209 4661 4747 4759
Size: 252 216 367 385 17 1452 86 12
Cuts at: 4759 5105 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 252 216 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4558
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4371
Fragments arranged by size:
4371 683 56
BtsI GCAGTG_nn'
Cuts at: 2626 3420 3440 2626
Size: 794 20 4296
Fragments arranged by size:
4296 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5075
DraI TTT'AAA
Cuts at: 2414 2414
Size: 5110
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4117 809
Size: 507 2801 1802
Fragments arranged by size:
2801 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2557 3446 5075
Size: 54 1425 50 275 18 727 889 1629
Cuts at: 5075 8
Size: 43
Fragments arranged by size:
1629 1425 889 727 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2557 1537
Size: 275 745 4090
Fragments arranged by size:
4090 745 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3040 1353
Size: 1687 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3697 4071 4217 387
Size: 1064 138 2108 374 146 1280
Fragments arranged by size:
2108 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2270 2378 3173 3770 4557 4825
Size: 393 503 135 108 795 597 787 268
Cuts at: 4825 1239
Size: 1524
Fragments arranged by size:
1524 795 787 597 503 393 268 135
108
EcoRI G'AATT_C
Cuts at: 2419 2797 2419
Size: 378 4732
FspI TGC'GCA
Cuts at: 2096 3616 2096
Size: 1520 3590
HaeII r_GCGC'y
Cuts at: 1678 4257 1678
Size: 2579 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3751 3783 3825 3857 1373
Size: 32 135 32 2179 32 42 32 2626
Fragments arranged by size:
2626 2179 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2014 2044 2454 2700 669
Size: 988 281 76 30 410 246 3079
Fragments arranged by size:
3079 988 410 281 246 76 30
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5110
HpaI GTT'AAC
Cuts at: 2700 2700
Size: 5110
KpnI G_GTAC'C
Cuts at: 2435 2435
Size: 5110
MfeI C'AATT_G
Cuts at: 2709 2709
Size: 5110
MluI A'CGCG_T
Cuts at: 4986 4986
Size: 5110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4224 4408 1305
Size: 2919 184 2007
Fragments arranged by size:
2919 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3288
Fragments arranged by size:
3288 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3068 3427 3586 518
Size: 648 1902 359 159 2042
Fragments arranged by size:
2042 1902 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2526 3203 4351 4596 1606
Size: 920 677 1148 245 2120
Fragments arranged by size:
2120 1148 920 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4558
NdeI CA'TA_TG
Cuts at: 387 2151 387
Size: 1764 3346
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5110
NotI GC'GGCC_GC
Cuts at: 2557 2557
Size: 5110
NspI r_CATG'y
Cuts at: 1132 1229 2468 4013 1132
Size: 97 1239 1545 2229
Fragments arranged by size:
2229 1545 1239 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5110
PciI A'CATG_T
Cuts at: 4009 4009
Size: 5110
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5110
PmeI GTTT'AAAC
Cuts at: 2414 2414
Size: 5110
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 4692 4724 3179
Size: 32 1481 32 3565
Fragments arranged by size:
3565 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5110
PsiI TTA'TAA
Cuts at: 2680 2680
Size: 5110
PstI C_TGCA'G
Cuts at: 830 2462 830
Size: 1632 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5110
SalI G'TCGA_C
Cuts at: 1936 2012 2452 1936
Size: 76 440 4594
Fragments arranged by size:
4594 440 76
SbfI CC_TGCA'GG
Cuts at: 2462 2462
Size: 5110
ScaI AGT'ACT
Cuts at: 3358 5070 3358
Size: 1712 3398
SfcI C'TryA_G
Cuts at: 826 1045 2458 3593 4274 4465 826
Size: 219 1413 1135 681 191 1471
Fragments arranged by size:
1471 1413 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5110
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5110
SmaI CCC'GGG
Cuts at: 1872 2437 1872
Size: 565 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3214 4115 4377 820
Size: 197 926 1271 901 262 1553
Fragments arranged by size:
1553 1271 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2901 493
Size: 2408 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5110
SphI G_CATG'C
Cuts at: 1132 2468 1132
Size: 1336 3774
SspI AAT'ATT
Cuts at: 5 52 3034 5
Size: 47 2982 2081
Fragments arranged by size:
2982 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5110
StyI C'CwwG_G
Cuts at: 513 1065 2549 513
Size: 552 1484 3074
Fragments arranged by size:
3074 1484 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3137 3154 3307 3492 3937 325
Size: 1255 1557 17 153 185 445 1498
Fragments arranged by size:
1557 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3356 5068 96
Size: 274 80 33 51 1443 1379 1712 138
Fragments arranged by size:
1712 1443 1379 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2326 2358 5070 5102 2326
Size: 32 2712 32 2334
Fragments arranged by size:
2712 2334 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5110
XbaI T'CTAG_A
Cuts at: 2446 2446
Size: 5110
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5110
XmaI C'CCGG_G
Cuts at: 1870 2435 1870
Size: 565 4545
XmnI GAAnn'nnTTC
Cuts at: 3239 3239
Size: 5110
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2080 2918 276
Size: 53 83 186 936 546 838 2468
Fragments arranged by size:
2468 936 838 546 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI