Restriction map for
pFN21AB6721
(Circular) (Six-base) MAPSORT of: pFN21AB6721.seq Check: 8923 from: 1 to: 4978
pFN21AB6721 4978 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2788 278
Size: 53 83 186 936 1252 2468
Fragments arranged by size:
2468 1252 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2321 1937
Size: 384 4594
Acc65I G'GTAC_C
Cuts at: 2299 2299
Size: 4978
AclI AA'CG_TT
Cuts at: 1563 3105 3478 1563
Size: 1542 373 3063
Fragments arranged by size:
3063 1542 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2292 3041 4425 4693 1632
Size: 660 749 1384 268 1917
Fragments arranged by size:
1917 1384 749 660 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4781
AflIII A'CryG_T
Cuts at: 1203 3877 4854 1203
Size: 2674 977 1327
Fragments arranged by size:
2674 1327 977
AgeI A'CCGG_T
Cuts at: 3809 3809
Size: 4978
AhdI GACnn_n'nnGTC
Cuts at: 3707 3707
Size: 4978
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4978
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4978
AlwNI CAG_nnn'CTG
Cuts at: 2041 2721 4293 2041
Size: 680 1572 2726
Fragments arranged by size:
2726 1572 680
ApaLI G'TGCA_C
Cuts at: 3035 4191 3035
Size: 1156 3822
ApoI r'AATT_y
Cuts at: 1493 2079 2287 2513 2665 4774 1493
Size: 586 208 226 152 2109 1697
Fragments arranged by size:
2109 1697 586 226 208 152
AseI AT'TA_AT
Cuts at: 160 1032 3532 160
Size: 872 2500 1606
Fragments arranged by size:
2500 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4978
AvaI C'yCGr_G
Cuts at: 1870 1943 2303 1870
Size: 73 360 4545
Fragments arranged by size:
4545 360 73
BamHI G'GATC_C
Cuts at: 2308 2675 2308
Size: 367 4611
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2299 3754 618
Size: 325 217 627 512 1455 1842
Fragments arranged by size:
1842 1455 627 512 325 217
BanII G_rGCy'C
Cuts at: 1391 2297 1391
Size: 906 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3169 3203 1903
Size: 34 1232 34 3678
Fragments arranged by size:
3678 1232 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2870 4080 2870
Size: 1210 3768
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4978
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3589 136
Size: 107 122 71 631 605 337 1580 1525
Fragments arranged by size:
1580 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 4973 4973
Size: 4978
BlpI GC'TnA_GC
Cuts at: 2395 2395
Size: 4978
Bme1580I G_kGCm'C
Cuts at: 1790 3039 4195 1790
Size: 1249 1156 2573
Fragments arranged by size:
2573 1249 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4978
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2709 3667 442
Size: 416 53 83 399 24 1292 958 1753
Fragments arranged by size:
1753 1292 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2277 3638 1239
Size: 1038 1361 2579
Fragments arranged by size:
2579 1361 1038
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3103 3968 4266 3103
Size: 865 298 3815
Fragments arranged by size:
3815 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3641 882
Size: 617 2142 2219
Fragments arranged by size:
2219 2142 617
BsaAI yAC'GTr
Cuts at: 493 1204 2769 493
Size: 711 1565 2702
Fragments arranged by size:
2702 1565 711
BsaBI GATnn'nnATC
Cuts at: 4972 4972
Size: 4978
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2785 3167 3802 275
Size: 53 83 186 936 1252 382 635 1451
Fragments arranged by size:
1451 1252 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3411 3809 4083 4230 4641 3411
Size: 398 274 147 411 3748
Fragments arranged by size:
3748 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4753
Fragments arranged by size:
4753 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2274 1186
Size: 1088 3890
BseYI C'CCAG_C
Cuts at: 4181 4677 4181
Size: 496 4482
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2428 3189 4217 1540
Size: 275 186 427 761 1028 2301
Fragments arranged by size:
2301 1028 761 427 275 186
BsiHKAI G_wGCw'C
Cuts at: 3039 3124 4195 3039
Size: 85 1071 3822
Fragments arranged by size:
3822 1071 85
BsmI GAATG_Cn'
Cuts at: 2489 2582 2489
Size: 93 4885
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2297 3039 3124 4195 1391
Size: 399 507 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 507 399 85
BspEI T'CCGG_A
Cuts at: 4641 4641
Size: 4978
BspHI T'CATG_A
Cuts at: 2867 4597 2867
Size: 1730 3248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2333 844
Size: 1489 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2865 1394
Size: 429 1042 3507
Fragments arranged by size:
3507 1042 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3473 3647 65
Size: 1183 2225 174 1396
Fragments arranged by size:
2225 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3622 3809 1700
Size: 110 1812 187 2869
Fragments arranged by size:
2869 1812 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4978
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4978
BssSI C'ACGA_G
Cuts at: 3038 4050 3038
Size: 1012 3966
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2041 794
Size: 1247 3731
BstBI TT'CG_AA
Cuts at: 3782 3782
Size: 4978
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4978
BstYI r'GATC_y
Cuts at: 1972 2308 2675 3060 3077 4529 4615 4627 4973
Size: 336 367 385 17 1452 86 12 346
Cuts at: 4973 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 336 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3487
Fragments arranged by size:
3487 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4239
Fragments arranged by size:
4239 683 56
BtsI GCAGTG_nn'
Cuts at: 2494 3288 3308 2494
Size: 794 20 4164
Fragments arranged by size:
4164 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4978
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4943
DraI TTT'AAA
Cuts at: 2282 2282
Size: 4978
DraIII CAC_nnn'GTG
Cuts at: 2089 2089
Size: 4978
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3985 809
Size: 507 2669 1802
Fragments arranged by size:
2669 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2425 3314 4943
Size: 54 1425 50 275 18 595 889 1629
Cuts at: 4943 8
Size: 43
Fragments arranged by size:
1629 1425 889 595 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2425 1537
Size: 275 613 4090
Fragments arranged by size:
4090 613 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 2127 2908 1353
Size: 737 37 781 3423
Fragments arranged by size:
3423 781 737 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3565 3939 4085 387
Size: 1064 138 1976 374 146 1280
Fragments arranged by size:
1976 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 2292 3041 3638 4425 4693 1239
Size: 393 645 15 749 597 787 268 1524
Fragments arranged by size:
1524 787 749 645 597 393 268 15
EcoO109I rG'GnC_Cy
Cuts at: 2267 2267
Size: 4978
EcoRI G'AATT_C
Cuts at: 2079 2287 2665 2079
Size: 208 378 4392
Fragments arranged by size:
4392 378 208
FspI TGC'GCA
Cuts at: 3484 3484
Size: 4978
HaeII r_GCGC'y
Cuts at: 1678 4125 1678
Size: 2447 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3619 3651 3693 3725 1373
Size: 32 135 32 2047 32 42 32 2626
Fragments arranged by size:
2626 2047 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2322 2568 669
Size: 988 281 384 246 3079
Fragments arranged by size:
3079 988 384 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4978
HpaI GTT'AAC
Cuts at: 2568 2568
Size: 4978
KpnI G_GTAC'C
Cuts at: 2303 2303
Size: 4978
MfeI C'AATT_G
Cuts at: 2577 2577
Size: 4978
MluI A'CGCG_T
Cuts at: 4854 4854
Size: 4978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4092 4276 1305
Size: 2787 184 2007
Fragments arranged by size:
2787 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3156
Fragments arranged by size:
3156 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2936 3295 3454 518
Size: 648 924 846 359 159 2042
Fragments arranged by size:
2042 924 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2394 3071 4219 4464 1606
Size: 432 356 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 432 356 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3487
Fragments arranged by size:
3487 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4978
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4978
NotI GC'GGCC_GC
Cuts at: 2425 2425
Size: 4978
NspI r_CATG'y
Cuts at: 1132 1229 2336 3881 1132
Size: 97 1107 1545 2229
Fragments arranged by size:
2229 1545 1107 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4978
PciI A'CATG_T
Cuts at: 3877 3877
Size: 4978
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4978
PfoI T'CCnGG_A
Cuts at: 2254 2254
Size: 4978
PmeI GTTT'AAAC
Cuts at: 2282 2282
Size: 4978
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3047 3079 4560 4592 3047
Size: 32 1481 32 3433
Fragments arranged by size:
3433 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2267 2267
Size: 4978
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4978
PsiI TTA'TAA
Cuts at: 2548 2548
Size: 4978
PstI C_TGCA'G
Cuts at: 830 2330 830
Size: 1500 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4978
PvuII CAG'CTG
Cuts at: 2038 2038
Size: 4978
SalI G'TCGA_C
Cuts at: 1936 2320 1936
Size: 384 4594
SbfI CC_TGCA'GG
Cuts at: 2330 2330
Size: 4978
ScaI AGT'ACT
Cuts at: 3226 4938 3226
Size: 1712 3266
SfcI C'TryA_G
Cuts at: 826 1045 2326 3461 4142 4333 826
Size: 219 1281 1135 681 191 1471
Fragments arranged by size:
1471 1281 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4978
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4978
SmaI CCC'GGG
Cuts at: 1872 2305 1872
Size: 433 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3082 3983 4245 820
Size: 197 926 1139 901 262 1553
Fragments arranged by size:
1553 1139 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2769 493
Size: 2276 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4978
SphI G_CATG'C
Cuts at: 1132 2336 1132
Size: 1204 3774
SspI AAT'ATT
Cuts at: 5 52 2902 5
Size: 47 2850 2081
Fragments arranged by size:
2850 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4978
StyI C'CwwG_G
Cuts at: 513 1065 2004 2417 513
Size: 552 939 413 3074
Fragments arranged by size:
3074 939 552 413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3005 3022 3175 3360 3805 325
Size: 1255 1425 17 153 185 445 1498
Fragments arranged by size:
1498 1425 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3224 4936 96
Size: 274 80 33 51 1443 1247 1712 138
Fragments arranged by size:
1712 1443 1247 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2259 2291 4938 4970 2259
Size: 32 2647 32 2267
Fragments arranged by size:
2647 2267 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2141 1316
Size: 825 4153
XbaI T'CTAG_A
Cuts at: 2314 2314
Size: 4978
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4978
XmaI C'CCGG_G
Cuts at: 1870 2303 1870
Size: 433 4545
XmnI GAAnn'nnTTC
Cuts at: 3107 3107
Size: 4978
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2786 276
Size: 53 83 186 936 1252 2468
Fragments arranged by size:
2468 1252 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI