Restriction map for
pFN21AB6719
(Circular) (Six-base) MAPSORT of: pFN21AB6719.seq Check: 8253 from: 1 to: 4957
pFN21AB6719 4957 bp
With 182 enzymes: SgfI *
October 20, 2009 10:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2767 278
Size: 53 83 186 936 1231 2468
Fragments arranged by size:
2468 1231 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2300 1937
Size: 363 4594
Acc65I G'GTAC_C
Cuts at: 2278 2278
Size: 4957
AclI AA'CG_TT
Cuts at: 1563 3084 3457 1563
Size: 1521 373 3063
Fragments arranged by size:
3063 1521 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2224 3020 4404 4672 1632
Size: 592 796 1384 268 1917
Fragments arranged by size:
1917 1384 796 592 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4760
AflIII A'CryG_T
Cuts at: 1203 3856 4833 1203
Size: 2653 977 1327
Fragments arranged by size:
2653 1327 977
AgeI A'CCGG_T
Cuts at: 3788 3788
Size: 4957
AhdI GACnn_n'nnGTC
Cuts at: 3686 3686
Size: 4957
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4957
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4957
AlwNI CAG_nnn'CTG
Cuts at: 2700 4272 2700
Size: 1572 3385
ApaLI G'TGCA_C
Cuts at: 3014 4170 3014
Size: 1156 3801
ApoI r'AATT_y
Cuts at: 1493 2266 2492 2644 4753 1493
Size: 773 226 152 2109 1697
Fragments arranged by size:
2109 1697 773 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3511 160
Size: 872 2479 1606
Fragments arranged by size:
2479 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4957
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 1870
Size: 73 339 4545
Fragments arranged by size:
4545 339 73
BamHI G'GATC_C
Cuts at: 2287 2654 2287
Size: 367 4590
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2278 3733 618
Size: 325 217 627 491 1455 1842
Fragments arranged by size:
1842 1455 627 491 325 217
BanII G_rGCy'C
Cuts at: 1391 2087 2276 1391
Size: 696 189 4072
Fragments arranged by size:
4072 696 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3986
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3148 3182 1903
Size: 34 50 34 1127 34 3678
Fragments arranged by size:
3678 1127 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2849 4059 2849
Size: 1210 3747
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4957
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3568 136
Size: 107 122 71 631 605 1896 1525
Fragments arranged by size:
1896 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4952 4952
Size: 4957
BlpI GC'TnA_GC
Cuts at: 2374 2374
Size: 4957
Bme1580I G_kGCm'C
Cuts at: 1790 3018 4174 1790
Size: 1228 1156 2573
Fragments arranged by size:
2573 1228 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4957
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2688 3646 442
Size: 416 53 83 399 24 1271 958 1753
Fragments arranged by size:
1753 1271 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2022 3617 1239
Size: 783 1595 2579
Fragments arranged by size:
2579 1595 783
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3082 3947 4245 3082
Size: 865 298 3794
Fragments arranged by size:
3794 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3620 882
Size: 617 2121 2219
Fragments arranged by size:
2219 2121 617
BsaAI yAC'GTr
Cuts at: 493 1204 2748 493
Size: 711 1544 2702
Fragments arranged by size:
2702 1544 711
BsaBI GATnn'nnATC
Cuts at: 4951 4951
Size: 4957
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2764 3146 3781 275
Size: 53 83 186 936 1231 382 635 1451
Fragments arranged by size:
1451 1231 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3390 3788 4062 4209 4620 3390
Size: 398 274 147 411 3727
Fragments arranged by size:
3727 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4732
Fragments arranged by size:
4732 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 1186
Size: 874 4083
BseYI C'CCAG_C
Cuts at: 4160 4656 4160
Size: 496 4461
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 1163
Size: 573 363 4021
Fragments arranged by size:
4021 573 363
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2407 3168 4196 1540
Size: 275 186 406 761 1028 2301
Fragments arranged by size:
2301 1028 761 406 275 186
BsiHKAI G_wGCw'C
Cuts at: 3018 3103 4174 3018
Size: 85 1071 3801
Fragments arranged by size:
3801 1071 85
BsmI GAATG_Cn'
Cuts at: 2468 2561 2468
Size: 93 4864
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2276 3018 3103 4174 1391
Size: 399 297 189 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 297 189 85
BspEI T'CCGG_A
Cuts at: 4620 4620
Size: 4957
BspHI T'CATG_A
Cuts at: 2846 4576 2846
Size: 1730 3227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2312 844
Size: 1468 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2844 1394
Size: 429 1021 3507
Fragments arranged by size:
3507 1021 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3452 3626 65
Size: 1183 2204 174 1396
Fragments arranged by size:
2204 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3601 3788 1700
Size: 110 1791 187 2869
Fragments arranged by size:
2869 1791 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4957
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4957
BssSI C'ACGA_G
Cuts at: 3017 4029 3017
Size: 1012 3945
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4957
BstBI TT'CG_AA
Cuts at: 3761 3761
Size: 4957
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4957
BstYI r'GATC_y
Cuts at: 1972 2020 2287 2654 3039 3056 4508 4594 4606
Size: 48 267 367 385 17 1452 86 12
Cuts at: 4606 4952 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 267 86 48
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2136 513
Size: 552 1071 3334
Fragments arranged by size:
3334 1071 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4218
Fragments arranged by size:
4218 683 56
BtsI GCAGTG_nn'
Cuts at: 2473 3267 3287 2473
Size: 794 20 4143
Fragments arranged by size:
4143 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4957
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4922
DraI TTT'AAA
Cuts at: 2261 2261
Size: 4957
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3964 809
Size: 507 2648 1802
Fragments arranged by size:
2648 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2404 3293 4922
Size: 54 1425 50 275 18 574 889 1629
Cuts at: 4922 8
Size: 43
Fragments arranged by size:
1629 1425 889 574 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2404 1537
Size: 275 592 4090
Fragments arranged by size:
4090 592 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 2045 2887 1353
Size: 684 8 842 3423
Fragments arranged by size:
3423 842 684 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3544 3918 4064 387
Size: 1064 138 1955 374 146 1280
Fragments arranged by size:
1955 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2022 2224 3020 3617 4404 4672 1239
Size: 393 390 202 796 597 787 268 1524
Fragments arranged by size:
1524 796 787 597 393 390 268 202
EcoNI CCTnn'n_nnAGG
Cuts at: 2205 2242 2205
Size: 37 4920
EcoRI G'AATT_C
Cuts at: 2266 2644 2266
Size: 378 4579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2237 2237
Size: 4957
FspI TGC'GCA
Cuts at: 3463 3463
Size: 4957
HaeII r_GCGC'y
Cuts at: 1678 4104 1678
Size: 2426 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3598 3630 3672 3704 1373
Size: 32 135 32 2026 32 42 32 2626
Fragments arranged by size:
2626 2026 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2301 2547 669
Size: 988 281 363 246 3079
Fragments arranged by size:
3079 988 363 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4957
HpaI GTT'AAC
Cuts at: 2547 2547
Size: 4957
KpnI G_GTAC'C
Cuts at: 2282 2282
Size: 4957
MfeI C'AATT_G
Cuts at: 2556 2556
Size: 4957
MluI A'CGCG_T
Cuts at: 4833 4833
Size: 4957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4071 4255 1305
Size: 2766 184 2007
Fragments arranged by size:
2766 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3135
Fragments arranged by size:
3135 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2105 2915 3274 3433 518
Size: 648 939 810 359 159 2042
Fragments arranged by size:
2042 939 810 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2373 3050 4198 4443 1606
Size: 767 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4405
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4957
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4957
NotI GC'GGCC_GC
Cuts at: 2404 2404
Size: 4957
NspI r_CATG'y
Cuts at: 1132 1229 2315 3860 1132
Size: 97 1086 1545 2229
Fragments arranged by size:
2229 1545 1086 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4957
PciI A'CATG_T
Cuts at: 3856 3856
Size: 4957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4957
PmeI GTTT'AAAC
Cuts at: 2261 2261
Size: 4957
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3026 3058 4539 4571 3026
Size: 32 1481 32 3412
Fragments arranged by size:
3412 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4957
PsiI TTA'TAA
Cuts at: 2527 2527
Size: 4957
PstI C_TGCA'G
Cuts at: 830 2309 830
Size: 1479 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4957
SalI G'TCGA_C
Cuts at: 1936 2299 1936
Size: 363 4594
SbfI CC_TGCA'GG
Cuts at: 2309 2309
Size: 4957
ScaI AGT'ACT
Cuts at: 3205 4917 3205
Size: 1712 3245
SfcI C'TryA_G
Cuts at: 826 1045 2202 2305 3440 4121 4312 826
Size: 219 1157 103 1135 681 191 1471
Fragments arranged by size:
1471 1157 1135 681 219 191 103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4957
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4957
SmaI CCC'GGG
Cuts at: 1872 2284 1872
Size: 412 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3061 3962 4224 820
Size: 197 926 1118 901 262 1553
Fragments arranged by size:
1553 1118 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2748 493
Size: 2255 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4957
SphI G_CATG'C
Cuts at: 1132 2315 1132
Size: 1183 3774
SspI AAT'ATT
Cuts at: 5 52 2881 5
Size: 47 2829 2081
Fragments arranged by size:
2829 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4957
StyI C'CwwG_G
Cuts at: 513 1065 2396 513
Size: 552 1331 3074
Fragments arranged by size:
3074 1331 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2984 3001 3154 3339 3784 325
Size: 1255 1404 17 153 185 445 1498
Fragments arranged by size:
1498 1404 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3203 4915 96
Size: 274 80 33 51 1443 1226 1712 138
Fragments arranged by size:
1712 1443 1226 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4917 4949 4917
Size: 32 4925
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4957
XbaI T'CTAG_A
Cuts at: 2293 2293
Size: 4957
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4957
XmaI C'CCGG_G
Cuts at: 1870 2282 1870
Size: 412 4545
XmnI GAAnn'nnTTC
Cuts at: 3086 3086
Size: 4957
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2765 276
Size: 53 83 186 936 1231 2468
Fragments arranged by size:
2468 1231 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI