Restriction map for
pFN21AB6701
(Circular) (Six-base) MAPSORT of: pFN21AB6701.seq Check: 9134 from: 1 to: 4891
pFN21AB6701 4891 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2024 2057 2024
Size: 33 4858
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2701 278
Size: 53 83 186 936 1165 2468
Fragments arranged by size:
2468 1165 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2234 1937
Size: 297 4594
Acc65I G'GTAC_C
Cuts at: 2212 2212
Size: 4891
AclI AA'CG_TT
Cuts at: 1563 3018 3391 1563
Size: 1455 373 3063
Fragments arranged by size:
3063 1455 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2954 4338 4606 1632
Size: 1322 1384 268 1917
Fragments arranged by size:
1917 1384 1322 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4694
AflIII A'CryG_T
Cuts at: 1203 3790 4767 1203
Size: 2587 977 1327
Fragments arranged by size:
2587 1327 977
AgeI A'CCGG_T
Cuts at: 3722 3722
Size: 4891
AhdI GACnn_n'nnGTC
Cuts at: 3620 3620
Size: 4891
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4891
AlwNI CAG_nnn'CTG
Cuts at: 2061 2184 2634 4206 2061
Size: 123 450 1572 2746
Fragments arranged by size:
2746 1572 450 123
ApaLI G'TGCA_C
Cuts at: 2948 4104 2948
Size: 1156 3735
ApoI r'AATT_y
Cuts at: 1493 2200 2426 2578 4687 1493
Size: 707 226 152 2109 1697
Fragments arranged by size:
2109 1697 707 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3445 160
Size: 872 2413 1606
Fragments arranged by size:
2413 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4891
AvaI C'yCGr_G
Cuts at: 1870 1943 2216 1870
Size: 73 273 4545
Fragments arranged by size:
4545 273 73
BamHI G'GATC_C
Cuts at: 2221 2588 2221
Size: 367 4524
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2212 3667 618
Size: 325 217 627 425 1455 1842
Fragments arranged by size:
1842 1455 627 425 325 217
BanII G_rGCy'C
Cuts at: 1391 2210 1391
Size: 819 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3082 3116 1903
Size: 34 1145 34 3678
Fragments arranged by size:
3678 1145 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2783 3993 2783
Size: 1210 3681
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4891
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 3502 136
Size: 107 122 71 631 605 452 1378 1525
Fragments arranged by size:
1525 1378 631 605 452 122 107 71
BglII A'GATC_T
Cuts at: 4886 4886
Size: 4891
BlpI GC'TnA_GC
Cuts at: 2308 2308
Size: 4891
Bme1580I G_kGCm'C
Cuts at: 1790 2143 2952 4108 1790
Size: 353 809 1156 2573
Fragments arranged by size:
2573 1156 809 353
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4891
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2622 3580 442
Size: 416 53 83 399 24 1205 958 1753
Fragments arranged by size:
1753 1205 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3551 1239
Size: 2312 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3016 3881 4179 3016
Size: 865 298 3728
Fragments arranged by size:
3728 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3554 882
Size: 617 2055 2219
Fragments arranged by size:
2219 2055 617
BsaAI yAC'GTr
Cuts at: 493 1204 2682 493
Size: 711 1478 2702
Fragments arranged by size:
2702 1478 711
BsaBI GATnn'nnATC
Cuts at: 4885 4885
Size: 4891
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2698 3080 3715 275
Size: 53 83 186 936 1165 382 635 1451
Fragments arranged by size:
1451 1165 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3324 3722 3996 4143 4554 3324
Size: 398 274 147 411 3661
Fragments arranged by size:
3661 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4666
Fragments arranged by size:
4666 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4891
BseYI C'CCAG_C
Cuts at: 4094 4590 4094
Size: 496 4395
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2029 2197 1163
Size: 573 293 168 3857
Fragments arranged by size:
3857 573 293 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2341 3102 4130 1540
Size: 275 186 340 761 1028 2301
Fragments arranged by size:
2301 1028 761 340 275 186
BsiHKAI G_wGCw'C
Cuts at: 2952 3037 4108 2952
Size: 85 1071 3735
Fragments arranged by size:
3735 1071 85
BsmI GAATG_Cn'
Cuts at: 2402 2495 2402
Size: 93 4798
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2210 2952 3037 4108 1391
Size: 399 353 67 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 353 85 67
BspEI T'CCGG_A
Cuts at: 4554 4554
Size: 4891
BspHI T'CATG_A
Cuts at: 2780 4510 2780
Size: 1730 3161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2024 2057 2246 844
Size: 1180 33 189 3489
Fragments arranged by size:
3489 1180 189 33
BsrBI CCG'CTC
Cuts at: 1394 1823 2778 1394
Size: 429 955 3507
Fragments arranged by size:
3507 955 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3386 3560 65
Size: 1183 2138 174 1396
Fragments arranged by size:
2138 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3535 3722 1700
Size: 110 1725 187 2869
Fragments arranged by size:
2869 1725 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4891
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4891
BssSI C'ACGA_G
Cuts at: 2951 3963 2951
Size: 1012 3879
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2076 2091 2184 794
Size: 1282 15 93 3501
Fragments arranged by size:
3501 1282 93 15
BstBI TT'CG_AA
Cuts at: 3695 3695
Size: 4891
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4891
BstYI r'GATC_y
Cuts at: 1972 2221 2588 2973 2990 4442 4528 4540 4886
Size: 249 367 385 17 1452 86 12 346
Cuts at: 4886 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 249 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3400
Fragments arranged by size:
3400 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4152
Fragments arranged by size:
4152 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 3201 3221 2407
Size: 794 20 4077
Fragments arranged by size:
4077 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4856
DraI TTT'AAA
Cuts at: 2195 2195
Size: 4891
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3898 809
Size: 507 2582 1802
Fragments arranged by size:
2582 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2338 3227 4856
Size: 54 1425 50 275 18 508 889 1629
Cuts at: 4856 8
Size: 43
Fragments arranged by size:
1629 1425 889 508 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2338 1537
Size: 275 526 4090
Fragments arranged by size:
4090 526 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2821 1353
Size: 740 728 3423
Fragments arranged by size:
3423 740 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3478 3852 3998 387
Size: 1064 138 1889 374 146 1280
Fragments arranged by size:
1889 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2954 3551 4338 4606 1239
Size: 393 1322 597 787 268 1524
Fragments arranged by size:
1524 1322 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2200 2578 2200
Size: 378 4513
FspI TGC'GCA
Cuts at: 3397 3397
Size: 4891
HaeII r_GCGC'y
Cuts at: 1678 4038 1678
Size: 2360 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3532 3564 3606 3638 1373
Size: 32 135 32 1960 32 42 32 2626
Fragments arranged by size:
2626 1960 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2235 2481 669
Size: 988 281 297 246 3079
Fragments arranged by size:
3079 988 297 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4891
HpaI GTT'AAC
Cuts at: 2481 2481
Size: 4891
KpnI G_GTAC'C
Cuts at: 2216 2216
Size: 4891
MfeI C'AATT_G
Cuts at: 2490 2490
Size: 4891
MluI A'CGCG_T
Cuts at: 4767 4767
Size: 4891
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4005 4189 1305
Size: 2700 184 2007
Fragments arranged by size:
2700 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3069
Fragments arranged by size:
3069 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2849 3208 3367 518
Size: 648 1683 359 159 2042
Fragments arranged by size:
2042 1683 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2030 2181 2307 2984 4132 4377 1606
Size: 424 151 126 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 424 245 151 126
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3400
Fragments arranged by size:
3400 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4891
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4891
NotI GC'GGCC_GC
Cuts at: 2338 2338
Size: 4891
NspI r_CATG'y
Cuts at: 1132 1229 2249 3794 1132
Size: 97 1020 1545 2229
Fragments arranged by size:
2229 1545 1020 97
PasI CC'CwG_GG
Cuts at: 1348 2143 1348
Size: 795 4096
PciI A'CATG_T
Cuts at: 3790 3790
Size: 4891
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4891
PmeI GTTT'AAAC
Cuts at: 2195 2195
Size: 4891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 4473 4505 2960
Size: 32 1481 32 3346
Fragments arranged by size:
3346 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4891
PsiI TTA'TAA
Cuts at: 2461 2461
Size: 4891
PstI C_TGCA'G
Cuts at: 830 2035 2243 830
Size: 1205 208 3478
Fragments arranged by size:
3478 1205 208
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4891
PvuII CAG'CTG
Cuts at: 2181 2181
Size: 4891
SalI G'TCGA_C
Cuts at: 1936 2233 1936
Size: 297 4594
SapI GCTCTTCn'nnn_
Cuts at: 2093 2093
Size: 4891
SbfI CC_TGCA'GG
Cuts at: 2243 2243
Size: 4891
ScaI AGT'ACT
Cuts at: 3139 4851 3139
Size: 1712 3179
SfcI C'TryA_G
Cuts at: 826 1045 2031 2239 3374 4055 4246 826
Size: 219 986 208 1135 681 191 1471
Fragments arranged by size:
1471 1135 986 681 219 208 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4891
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4891
SmaI CCC'GGG
Cuts at: 1872 2218 1872
Size: 346 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2995 3896 4158 820
Size: 197 926 1052 901 262 1553
Fragments arranged by size:
1553 1052 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2682 493
Size: 2189 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4891
SphI G_CATG'C
Cuts at: 1132 2249 1132
Size: 1117 3774
SspI AAT'ATT
Cuts at: 5 52 2815 5
Size: 47 2763 2081
Fragments arranged by size:
2763 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4891
StyI C'CwwG_G
Cuts at: 513 1065 2004 2330 513
Size: 552 939 326 3074
Fragments arranged by size:
3074 939 552 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2918 2935 3088 3273 3718 325
Size: 1255 1338 17 153 185 445 1498
Fragments arranged by size:
1498 1338 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3137 4849 96
Size: 274 80 33 51 1443 1160 1712 138
Fragments arranged by size:
1712 1443 1160 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4851 4883 4851
Size: 32 4859
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4891
XbaI T'CTAG_A
Cuts at: 2227 2227
Size: 4891
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4891
XmaI C'CCGG_G
Cuts at: 1870 2216 1870
Size: 346 4545
XmnI GAAnn'nnTTC
Cuts at: 3020 3020
Size: 4891
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2699 276
Size: 53 83 186 936 1165 2468
Fragments arranged by size:
2468 1165 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI