Restriction map for
pFN21AB6698
(Circular) (Six-base) MAPSORT of: pFN21AB6698.seq Check: 4562 from: 1 to: 6040
pFN21AB6698 6040 bp
With 182 enzymes: SgfI *
October 20, 2009 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 6040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2343 3383 1937
Size: 406 1040 4594
Fragments arranged by size:
4594 1040 406
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 2488 2669 3107 4103 5487 5755 1632
Size: 610 246 181 438 996 1384 268 1917
Fragments arranged by size:
1917 1384 996 610 438 268 246 181
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 2148 4939 5916 1203
Size: 945 2791 977 1327
Fragments arranged by size:
2791 1327 977 945
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 3259 4769 3259
Size: 1510 4530
AleI CACnn'nnGTG
Cuts at: 1166 2540 1166
Size: 1374 4666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 3133 3783 5355 3133
Size: 650 1572 3818
Fragments arranged by size:
3818 1572 650
ApaLI G'TGCA_C
Cuts at: 2018 4097 5253 2018
Size: 2079 1156 2805
Fragments arranged by size:
2805 2079 1156
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 2731 2864 3365 1870
Size: 73 244 544 133 501 4545
Fragments arranged by size:
4545 544 501 244 133 73
BamHI G'GATC_C
Cuts at: 3036 3370 3737 3036
Size: 334 367 5339
Fragments arranged by size:
5339 367 334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2216 2447 2776 2860
Size: 325 217 627 267 162 231 329 84
Cuts at: 2860 3361 4816 618
Size: 501 1455 1842
Fragments arranged by size:
1842 1455 627 501 329 325 267 231
217 162 84
BanII G_rGCy'C
Cuts at: 1391 2550 2871 3359 1391
Size: 1159 321 488 4072
Fragments arranged by size:
4072 1159 488 321
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 6040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3059 928
Size: 971 1160 3909
Fragments arranged by size:
3909 1160 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2032 2066 3007 3041 4231
Size: 34 50 34 11 34 941 34 1190
Cuts at: 4231 4265 1903
Size: 34 3678
Fragments arranged by size:
3678 1190 941 50 34 34 34 34
34 11
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 4651 136
Size: 107 122 71 631 605 368 2611 1525
Fragments arranged by size:
2611 1525 631 605 368 122 107 71
BglII A'GATC_T
Cuts at: 2253 6035 2253
Size: 3782 2258
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2450 2781 2865 4101 5257 1790
Size: 232 428 331 84 1236 1156 2573
Fragments arranged by size:
2573 1236 1156 428 331 232 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 3771 4729
Size: 416 53 83 399 24 897 1457 958
Cuts at: 4729 442
Size: 1753
Fragments arranged by size:
1753 1457 958 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2228 2378 4700 1239
Size: 917 72 150 2322 2579
Fragments arranged by size:
2579 2322 917 150 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4165 5030 5328 4165
Size: 865 298 4877
Fragments arranged by size:
4877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3831 493
Size: 711 2627 2702
Fragments arranged by size:
2702 2627 711
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3285 3847 4229
Size: 53 83 186 936 522 1230 562 382
Cuts at: 4229 4864 275
Size: 635 1451
Fragments arranged by size:
1451 1230 936 635 562 522 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4473 4871 5145 5292 5703 4473
Size: 398 274 147 411 4810
Fragments arranged by size:
4810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5815
Fragments arranged by size:
5815 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3329 3332 3335 1186
Size: 2143 3 3 3891
Fragments arranged by size:
3891 2143 3 3
BseYI C'CCAG_C
Cuts at: 2874 5243 5739 2874
Size: 2369 496 3175
Fragments arranged by size:
3175 2369 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2777 3023 3173 1163
Size: 573 1041 246 150 4030
Fragments arranged by size:
4030 1041 573 246 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3060 3294 3490 4251 5279
Size: 275 186 928 131 234 196 761 1028
Cuts at: 5279 1540
Size: 2301
Fragments arranged by size:
2301 1028 928 761 275 234 196 186
131
BsiHKAI G_wGCw'C
Cuts at: 2022 2871 4101 4186 5257 2022
Size: 849 1230 85 1071 2805
Fragments arranged by size:
2805 1230 1071 849 85
BsmI GAATG_Cn'
Cuts at: 3551 3644 3551
Size: 93 5947
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2450 2550 2781 2865 2871 3359
Size: 399 232 428 100 231 84 6 488
Cuts at: 3359 4101 4186 5257 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 488 428 399 232 231
100 85 84 6
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 2493 3929 5659 2493
Size: 1436 1730 2874
Fragments arranged by size:
2874 1730 1436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 2760 3395 844
Size: 1882 34 635 3489
Fragments arranged by size:
3489 1882 635 34
BsrBI CCG'CTC
Cuts at: 1394 1823 2706 3927 1394
Size: 429 883 1221 3507
Fragments arranged by size:
3507 1221 883 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4535 4709 65
Size: 1183 3287 174 1396
Fragments arranged by size:
3287 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 4100 5112 4100
Size: 1012 5028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 2710 1184
Size: 1526 4514
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 2200
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2203 2253 2466 3036 3370 3737 4122 4139
Size: 231 50 213 570 334 367 385 17
Cuts at: 4139 5591 5677 5689 6035 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 570 385 367 346 334 231
213 86 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2113 2220 2923 3079 3288 513
Size: 552 1048 107 703 156 209 3265
Fragments arranged by size:
3265 1048 703 552 209 156 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 487
Size: 683 56 868 4433
Fragments arranged by size:
4433 868 683 56
BtsI GCAGTG_nn'
Cuts at: 3556 4350 4370 3556
Size: 794 20 5226
Fragments arranged by size:
5226 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DraIII CAC_nnn'GTG
Cuts at: 2689 2689
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 2281 2694
Size: 54 1425 50 275 18 418 33 413
Cuts at: 2694 2904 2926 3057 3096 3120 3162 3291 3487
Size: 210 22 131 39 24 42 129 196
Cuts at: 3487 4376 6005 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 418 413 275 210 196
131 129 54 50 43 42 39 33
24 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 3057 3291 3487 1537
Size: 275 1114 131 234 196 4090
Fragments arranged by size:
4090 1114 275 234 196 131
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 3970 1353
Size: 1116 1501 3423
Fragments arranged by size:
3423 1501 1116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2869 2869
Size: 6040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2228 2242 2378 2488 2669 3107
Size: 393 524 72 14 136 110 181 438
Cuts at: 3107 4103 4700 5487 5755 1239
Size: 996 597 787 268 1524
Fragments arranged by size:
1524 996 787 597 524 438 393 268
181 136 110 72 14
EcoO109I rG'GnC_Cy
Cuts at: 2620 2674 2620
Size: 54 5986
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FspI TGC'GCA
Cuts at: 2756 4546 2756
Size: 1790 4250
HaeII r_GCGC'y
Cuts at: 1678 2058 2855 5187 1678
Size: 380 797 2332 2531
Fragments arranged by size:
2531 2332 797 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2401 2433 3245 3277 4681
Size: 32 135 32 829 32 812 32 1404
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1404 829 812 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2344 3384 3630 669
Size: 988 281 406 1040 246 3079
Fragments arranged by size:
3079 1040 988 406 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4218
Fragments arranged by size:
4218 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 2540 2969 3164 3998 4357 4516
Size: 648 1032 342 429 195 834 359 159
Cuts at: 4516 518
Size: 2042
Fragments arranged by size:
2042 1032 834 648 429 359 342 195
159
MspA1I CmG'CkG
Cuts at: 1606 2134 2407 2578 2740 3081 3456 4133 5281
Size: 528 273 171 162 341 375 677 1148
Cuts at: 5281 5526 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 528 375 341 273 245
171 162
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 6040
NcoI C'CATG_G
Cuts at: 513 1065 2113 2220 513
Size: 552 1048 107 4333
Fragments arranged by size:
4333 1048 552 107
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 2968 3398 4943 1132
Size: 97 1739 430 1545 2229
Fragments arranged by size:
2229 1739 1545 430 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2064 2419 2578 2581 1108
Size: 956 355 159 3 4567
Fragments arranged by size:
4567 956 355 159 3
PfoI T'CCnGG_A
Cuts at: 2352 2438 2469 3126 2352
Size: 86 31 657 5266
Fragments arranged by size:
5266 657 86 31
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 4109 4141 5622 5654 3209
Size: 32 868 32 1481 32 3595
Fragments arranged by size:
3595 1481 868 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2674 2674
Size: 6040
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 6008
PstI C_TGCA'G
Cuts at: 830 3392 830
Size: 2562 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 2134 2407 2578 2740 2134
Size: 273 171 162 5434
Fragments arranged by size:
5434 273 171 162
SacI G_AGCT'C
Cuts at: 2871 2871
Size: 6040
SacII CC_GC'GG
Cuts at: 3082 3082
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 2342 3382 1936
Size: 406 1040 4594
Fragments arranged by size:
4594 1040 406
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SexAI A'CCwGG_T
Cuts at: 2685 3255 2685
Size: 570 5470
SfcI C'TryA_G
Cuts at: 826 1045 3388 4523 5204 5395 826
Size: 219 2343 1135 681 191 1471
Fragments arranged by size:
2343 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 6040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 2733 3367 1872
Size: 861 634 4545
Fragments arranged by size:
4545 861 634
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4144 5045 5307 820
Size: 197 926 2201 901 262 1553
Fragments arranged by size:
2201 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 3182 1517
Size: 1665 4375
StyI C'CwwG_G
Cuts at: 513 1065 2113 2220 2310 2523 3479 513
Size: 552 1048 107 90 213 956 3074
Fragments arranged by size:
3074 1048 956 552 213 107 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 2536 2557 2888 4067 4084 4237
Size: 1255 758 198 21 331 1179 17 153
Cuts at: 4237 4422 4867 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1179 758 445 331 198 185
153 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 4286 5998
Size: 274 80 33 51 1443 174 2135 1712
Cuts at: 5998 96
Size: 138
Fragments arranged by size:
2135 1712 1443 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 2348 1316
Size: 912 120 5008
Fragments arranged by size:
5008 912 120
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2899 2977 2899
Size: 78 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 2731 3365 1870
Size: 861 634 4545
Fragments arranged by size:
4545 861 634
XmnI GAAnn'nnTTC
Cuts at: 2501 4169 2501
Size: 1668 4372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI RsrII SanDI SapI SfiI SrfI
SwaI