Restriction map for
pFN21AB6694
(Circular) (Six-base) MAPSORT of: pFN21AB6694.seq Check: 9856 from: 1 to: 6091
pFN21AB6694 6091 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2763 3434 1937
Size: 826 671 4594
Fragments arranged by size:
4594 826 671
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 2856 2976 4218 4591 1563
Size: 1293 120 1242 373 3063
Fragments arranged by size:
3063 1293 1242 373 120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3149 4154 5538 5806 1632
Size: 1517 1005 1384 268 1917
Fragments arranged by size:
1917 1517 1384 1005 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 2047 4990 5967 1203
Size: 844 2943 977 1327
Fragments arranged by size:
2943 1327 977 844
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 2126 2240 2369 3179 3400 3626 3778 5887
Size: 633 114 129 810 221 226 152 2109
Cuts at: 5887 1493
Size: 1697
Fragments arranged by size:
2109 1697 810 633 226 221 152 129
114
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2403 3416 1870
Size: 73 460 1013 4545
Fragments arranged by size:
4545 1013 460 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3412 4867 618
Size: 325 217 627 1625 1455 1842
Fragments arranged by size:
1842 1625 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2162 3410 1391
Size: 771 1248 4072
Fragments arranged by size:
4072 1248 771
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2420 4702 136
Size: 107 122 71 631 605 748 2282 1525
Fragments arranged by size:
2282 1525 748 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2199 3123 3508 2199
Size: 924 385 4782
Fragments arranged by size:
4782 924 385
Bme1580I G_kGCm'C
Cuts at: 1790 4152 5308 1790
Size: 2362 1156 2573
Fragments arranged by size:
2573 2362 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2441 3822 4780
Size: 416 53 83 399 24 1024 1381 958
Cuts at: 4780 442
Size: 1753
Fragments arranged by size:
1753 1381 1024 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2925 3265 2925
Size: 340 5751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2630 2738 2832 3363 4751 1239
Size: 1391 108 94 531 1388 2579
Fragments arranged by size:
2579 1391 1388 531 108 94
Bpu10I CC'TnA_GC
Cuts at: 3030 3030
Size: 6091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2711 4216 5081 5379 2711
Size: 1505 865 298 3423
Fragments arranged by size:
3423 1505 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2240 4754 882
Size: 617 741 2514 2219
Fragments arranged by size:
2514 2219 741 617
BsaAI yAC'GTr
Cuts at: 493 1204 2050 3882 493
Size: 711 846 1832 2702
Fragments arranged by size:
2702 1832 846 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2830 2860 1178
Size: 30 165 30 1427 30 4409
Fragments arranged by size:
4409 1427 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6091
BseYI C'CCAG_C
Cuts at: 5294 5790 5294
Size: 496 5595
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3541 4302 5330 1540
Size: 275 186 1540 761 1028 2301
Fragments arranged by size:
2301 1540 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4152 4237 5308 4152
Size: 85 1071 4935
Fragments arranged by size:
4935 1071 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 3410 4152 4237 5308 1391
Size: 399 372 1248 742 85 1071 2174
Fragments arranged by size:
2174 1248 1071 742 399 372 85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2674 3105 4586 4760 65
Size: 1183 1426 431 1481 174 1396
Fragments arranged by size:
1481 1426 1396 1183 431 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3068 4735 4922 1700
Size: 110 1258 1667 187 2869
Fragments arranged by size:
2869 1667 1258 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 2836 3355 1184
Size: 1652 519 3920
Fragments arranged by size:
3920 1652 519
BstYI r'GATC_y
Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086
Size: 1449 367 385 17 1452 86 12 346
Cuts at: 6086 1972
Size: 1977
Fragments arranged by size:
1977 1452 1449 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3024 3330 3607 4401 4421 3024
Size: 306 277 794 20 4694
Fragments arranged by size:
4694 794 306 277 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2656 2671 2691 2706 2755 2790 529
Size: 35 2092 15 20 15 49 35 3830
Fragments arranged by size:
3830 2092 49 35 35 20 15 15
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DraIII CAC_nnn'GTG
Cuts at: 2435 2435
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2339 5098 809
Size: 507 1023 2759 1802
Fragments arranged by size:
2759 1802 1023 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2077 2797 3538
Size: 54 1425 50 275 18 247 720 741
Cuts at: 3538 4427 6056 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 741 720 275 247 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2492 2916 4021 1353
Size: 1139 424 1105 3423
Fragments arranged by size:
3423 1139 1105 424
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2630 2738 2832 3149 3363 4154 4751
Size: 393 998 108 94 317 214 791 597
Cuts at: 4751 5538 5806 1239
Size: 787 268 1524
Fragments arranged by size:
1524 998 791 787 597 393 317 268
214 108 94
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
EcoRV GAT'ATC
Cuts at: 2227 2227
Size: 6091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 6091
FspI TGC'GCA
Cuts at: 4597 4597
Size: 6091
HaeII r_GCGC'y
Cuts at: 1678 3069 5238 1678
Size: 1391 2169 2531
Fragments arranged by size:
2531 2169 1391
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2283 2315 2634 2666 4732
Size: 32 135 32 711 32 319 32 2066
Cuts at: 4732 4764 4806 4838 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2066 711 319 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2461 2854 3435 3681 669
Size: 988 281 523 393 581 246 3079
Fragments arranged by size:
3079 988 581 523 393 281 246
HindIII A'AGCT_T
Cuts at: 748 2895 748
Size: 2147 3944
HpaI GTT'AAC
Cuts at: 2461 3681 2461
Size: 1220 4871
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2570 2750 5205 5389 1305
Size: 1265 180 2455 184 2007
Fragments arranged by size:
2455 2007 1265 184 180
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2079 10
Size: 54 1425 343 247 4022
Fragments arranged by size:
4022 1425 343 247 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4049 4408 4567 518
Size: 648 2883 359 159 2042
Fragments arranged by size:
2883 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3017 3507 4184 5332 5577 1606
Size: 1411 490 677 1148 245 2120
Fragments arranged by size:
2120 1411 1148 677 490 245
NaeI GCC'GGC
Cuts at: 3070 3070
Size: 6091
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NgoMIV G'CCGG_C
Cuts at: 3068 3068
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2414 2608 2629 3114 3449 4994 1132
Size: 97 1185 194 21 485 335 1545 2229
Fragments arranged by size:
2229 1545 1185 485 335 194 97 21
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2585 1108
Size: 1477 4614
PfoI T'CCnGG_A
Cuts at: 2754 2754
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 3017 3017
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SapI GCTCTTCn'nnn_
Cuts at: 2916 2916
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 2258 4339 6051 2258
Size: 2081 1712 2298
Fragments arranged by size:
2298 2081 1712
SfcI C'TryA_G
Cuts at: 826 1045 2488 3439 4574 5255 5446 826
Size: 219 1443 951 1135 681 191 1471
Fragments arranged by size:
1471 1443 1135 951 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2690 4195 5096 5358 820
Size: 197 926 747 1505 901 262 1553
Fragments arranged by size:
1553 1505 926 901 747 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 2414 3114 3449 1132
Size: 1282 700 335 3774
Fragments arranged by size:
3774 1282 700 335
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 2004 2630 3530 513
Size: 552 939 626 900 3074
Fragments arranged by size:
3074 939 900 626 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2423 3299 4118 4135 4288 4473 4918
Size: 1255 843 876 819 17 153 185 445
Cuts at: 4918 325
Size: 1498
Fragments arranged by size:
1498 1255 876 843 819 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2256 4337 6049
Size: 274 80 33 51 1443 279 2081 1712
Cuts at: 6049 96
Size: 138
Fragments arranged by size:
2081 1712 1443 279 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2830 2862 6051 6083 2830
Size: 32 3189 32 2838
Fragments arranged by size:
3189 2838 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2586 3211 2586
Size: 625 5466
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 2910 4220 2910
Size: 1310 4781
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI