Restriction map for
pFN21AB6691
(Circular) (Six-base) MAPSORT of: pFN21AB6691.seq Check: 9590 from: 1 to: 5989
pFN21AB6691 5989 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2512 2512
Size: 5989
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 3310 3310
Size: 5989
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 2340 2436 2912 3008 4052 5436 5704
Size: 404 304 96 476 96 1044 1384 268
Cuts at: 5704 1632
Size: 1917
Fragments arranged by size:
1917 1384 1044 476 404 304 268 96
96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 4888 5865 1203
Size: 3685 977 1327
Fragments arranged by size:
3685 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 2625 1166
Size: 1459 4530
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5989
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 5957
AlwNI CAG_nnn'CTG
Cuts at: 2920 3732 5304 2920
Size: 812 1572 3605
Fragments arranged by size:
3605 1572 812
ApaI G_GGCC'C
Cuts at: 2661 2661
Size: 5989
ApaLI G'TGCA_C
Cuts at: 4046 5202 4046
Size: 1156 4833
ApoI r'AATT_y
Cuts at: 1493 3298 3524 3676 5785 1493
Size: 1805 226 152 2109 1697
Fragments arranged by size:
2109 1805 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2165 3314 1870
Size: 73 222 1149 4545
Fragments arranged by size:
4545 1149 222 73
BamHI G'GATC_C
Cuts at: 2104 3319 3686 2104
Size: 1215 367 4407
Fragments arranged by size:
4407 1215 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3310 4765 618
Size: 325 217 627 1523 1455 1842
Fragments arranged by size:
1842 1523 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2661 2709 3308 1391
Size: 1270 48 599 4072
Fragments arranged by size:
4072 1270 599 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2323 928
Size: 971 424 4594
Fragments arranged by size:
4594 971 424
BbvCI CC'TCA_GC
Cuts at: 2762 2762
Size: 5989
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3007 3041 4180 4214 1903
Size: 34 1070 34 1139 34 3678
Fragments arranged by size:
3678 1139 1070 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3881 5091 3881
Size: 1210 4779
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4600 136
Size: 107 122 71 631 605 2928 1525
Fragments arranged by size:
2928 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5984 5984
Size: 5989
BlpI GC'TnA_GC
Cuts at: 3406 3406
Size: 5989
Bme1580I G_kGCm'C
Cuts at: 1790 2661 4050 5206 1790
Size: 871 1389 1156 2573
Fragments arranged by size:
2573 1389 1156 871
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2595 3720 4678
Size: 416 53 83 399 24 1178 1125 958
Cuts at: 4678 442
Size: 1753
Fragments arranged by size:
1753 1178 1125 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2723 2108
Size: 615 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2411 4649 1239
Size: 1044 128 2238 2579
Fragments arranged by size:
2579 2238 1044 128
Bpu10I CC'TnA_GC
Cuts at: 2762 2762
Size: 5989
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4114 4979 5277 4114
Size: 865 298 4826
Fragments arranged by size:
4826 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3780 493
Size: 711 2576 2702
Fragments arranged by size:
2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 3270 3796 4178
Size: 53 83 186 936 609 1128 526 382
Cuts at: 4178 4813 275
Size: 635 1451
Fragments arranged by size:
1451 1128 936 635 609 526 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5764
Fragments arranged by size:
5764 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2717 2759 1186
Size: 1531 42 4416
Fragments arranged by size:
4416 1531 42
BseYI C'CCAG_C
Cuts at: 5192 5688 5192
Size: 496 5493
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5416
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2155 3439 4200 5228 1540
Size: 275 186 154 1284 761 1028 2301
Fragments arranged by size:
2301 1284 1028 761 275 186 154
BsiHKAI G_wGCw'C
Cuts at: 2353 2709 2997 4050 4135 5206 2353
Size: 356 288 1053 85 1071 3136
Fragments arranged by size:
3136 1071 1053 356 288 85
BsmI GAATG_Cn'
Cuts at: 2267 3269 3500 3593 2267
Size: 1002 231 93 4663
Fragments arranged by size:
4663 1002 231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2353 2661 2709 2997 3308 4050 4135
Size: 399 563 308 48 288 311 742 85
Cuts at: 4135 5206 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 563 399 311 308 288
85 48
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 2466 3878 5608 2466
Size: 1412 1730 2847
Fragments arranged by size:
2847 1730 1412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2512 2905 3344 844
Size: 1668 393 439 3489
Fragments arranged by size:
3489 1668 439 393
BsrBI CCG'CTC
Cuts at: 1394 1823 2100 3876 1394
Size: 429 277 1776 3507
Fragments arranged by size:
3507 1776 429 277
BsrDI GCAATG_nn'
Cuts at: 65 1248 3177 4484 4658 65
Size: 1183 1929 1307 174 1396
Fragments arranged by size:
1929 1396 1307 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4633 4820 1700
Size: 110 2823 187 2869
Fragments arranged by size:
2869 2823 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 2059 1925
Size: 134 5855
BssSI C'ACGA_G
Cuts at: 4049 5061 4049
Size: 1012 4977
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5989
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5989
BstYI r'GATC_y
Cuts at: 1972 2104 3319 3686 4071 4088 5540 5626 5638
Size: 132 1215 367 385 17 1452 86 12
Cuts at: 5638 5984 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1215 385 367 346 132 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2435 2435
Size: 5989
BtgI C'CryG_G
Cuts at: 513 1065 2004 3006 3066 513
Size: 552 939 1002 60 3436
Fragments arranged by size:
3436 1002 939 552 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2200 487
Size: 683 56 974 4276
Fragments arranged by size:
4276 974 683 56
BtsI GCAGTG_nn'
Cuts at: 3505 4299 4319 3505
Size: 794 20 5175
Fragments arranged by size:
5175 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DraIII CAC_nnn'GTG
Cuts at: 2124 2124
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4996 809
Size: 507 3680 1802
Fragments arranged by size:
3680 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2243 2922 3436
Size: 54 1425 50 275 18 413 679 514
Cuts at: 3436 4325 5954 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 679 514 413 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 3919 1353
Size: 746 1820 3423
Fragments arranged by size:
3423 1820 746
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4576 4950 5096 387
Size: 1064 138 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2707 2707
Size: 5989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2283 2340 2411 2436 2912 3008
Size: 393 404 247 57 71 25 476 96
Cuts at: 3008 4052 4649 5436 5704 1239
Size: 1044 597 787 268 1524
Fragments arranged by size:
1524 1044 787 597 476 404 393 268
247 96 71 57 25
EcoNI CCTnn'n_nnAGG
Cuts at: 3035 3035
Size: 5989
EcoO109I rG'GnC_Cy
Cuts at: 2431 2658 2431
Size: 227 5762
EcoRI G'AATT_C
Cuts at: 3298 3676 3298
Size: 378 5611
EcoRV GAT'ATC
Cuts at: 2294 2971 2294
Size: 677 5312
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 2752 5136 1678
Size: 1074 2384 2531
Fragments arranged by size:
2531 2384 1074
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 4630 4662 4704
Size: 32 135 32 806 32 2220 32 42
Cuts at: 4704 4736 1373
Size: 32 2626
Fragments arranged by size:
2626 2220 806 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3333 3579 669
Size: 988 281 1395 246 3079
Fragments arranged by size:
3079 1395 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2238 2369 748
Size: 1490 131 4368
Fragments arranged by size:
4368 1490 131
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 3314 3314
Size: 5989
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3015 5103 5287 1305
Size: 1710 2088 184 2007
Fragments arranged by size:
2088 2007 1710 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2245 2924 10
Size: 54 1425 343 413 679 3075
Fragments arranged by size:
3075 1425 679 413 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2625 2693 3947 4306 4465 518
Size: 648 1459 68 1254 359 159 2042
Fragments arranged by size:
2042 1459 1254 648 359 159 68
MspA1I CmG'CkG
Cuts at: 1606 2014 2074 2157 2497 2625 2761 2986 3405
Size: 408 60 83 340 128 136 225 419
Cuts at: 3405 4082 5230 5475 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 419 408 340 245 225
136 128 83 60
NcoI C'CATG_G
Cuts at: 513 1065 2004 3006 3066 513
Size: 552 939 1002 60 3436
Fragments arranged by size:
3436 1002 939 552 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 3160 3347 4892 1132
Size: 97 1931 187 1545 2229
Fragments arranged by size:
2229 1931 1545 187 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5989
PciI A'CATG_T
Cuts at: 4888 4888
Size: 5989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5989
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5989
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PspOMI G'GGCC_C
Cuts at: 2657 2657
Size: 5989
PstI C_TGCA'G
Cuts at: 830 3144 3341 830
Size: 2314 197 3478
Fragments arranged by size:
3478 2314 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 2497 2625 2761 2986 2497
Size: 128 136 225 5500
Fragments arranged by size:
5500 225 136 128
SacI G_AGCT'C
Cuts at: 2709 2709
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 2774 4237 5949 2774
Size: 1463 1712 2814
Fragments arranged by size:
2814 1712 1463
SfcI C'TryA_G
Cuts at: 826 1045 2215 3140 3337 4472 5153 5344 826
Size: 219 1170 925 197 1135 681 191 1471
Fragments arranged by size:
1471 1170 1135 925 681 219 197 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 3316 1872
Size: 1444 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4093 4994 5256 820
Size: 197 926 2150 901 262 1553
Fragments arranged by size:
2150 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SspI AAT'ATT
Cuts at: 5 52 2401 3913 5
Size: 47 2349 1512 2081
Fragments arranged by size:
2349 2081 1512 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5989
StyI C'CwwG_G
Cuts at: 513 1065 2004 2109 3006 3066 3428 513
Size: 552 939 105 897 60 362 3074
Fragments arranged by size:
3074 939 897 552 362 105 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4016 4033 4186 4371 4816 325
Size: 1255 2436 17 153 185 445 1498
Fragments arranged by size:
2436 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2772 4235 5947
Size: 274 80 33 51 1443 795 1463 1712
Cuts at: 5947 96
Size: 138
Fragments arranged by size:
1712 1463 1443 795 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5949 5981 5949
Size: 32 5957
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2719 2719
Size: 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 3314 1870
Size: 1444 4545
XmnI GAAnn'nnTTC
Cuts at: 2961 4118 2961
Size: 1157 4832
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstXI BstZ17I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI