Restriction map for
pFN21AB6689
(Circular) (Six-base) MAPSORT of: pFN21AB6689.seq Check: 4796 from: 1 to: 5710
pFN21AB6689 5710 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2989 3520 278
Size: 53 83 186 936 1453 531 2468
Fragments arranged by size:
2468 1453 936 531 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2256 3053 1937
Size: 319 797 4594
Fragments arranged by size:
4594 797 319
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 2958 3773 5157 5425 1632
Size: 824 502 815 1384 268 1917
Fragments arranged by size:
1917 1384 824 815 502 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 2927 3453 5025 2927
Size: 526 1572 3612
Fragments arranged by size:
3612 1572 526
ApaI G_GGCC'C
Cuts at: 2013 2013
Size: 5710
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 3019 3245 3397 5506 1493
Size: 1526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2829 4264 160
Size: 872 1797 1435 1606
Fragments arranged by size:
1797 1606 1435 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 3035 1870
Size: 73 400 692 4545
Fragments arranged by size:
4545 692 400 73
BamHI G'GATC_C
Cuts at: 2357 2604 3040 3407 2357
Size: 247 436 367 4660
Fragments arranged by size:
4660 436 367 247
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3031 4486 618
Size: 325 217 627 1244 1455 1842
Fragments arranged by size:
1842 1455 1244 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2898 3029 1391
Size: 622 885 131 4072
Fragments arranged by size:
4072 885 622 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2355 2981 928
Size: 971 456 626 3657
Fragments arranged by size:
3657 971 626 456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2948 2982 3901 3935 1903
Size: 34 1011 34 919 34 3678
Fragments arranged by size:
3678 1011 919 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BfrBI ATG'CAT
Cuts at: 2318 2318
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4321 136
Size: 107 122 71 631 605 2649 1525
Fragments arranged by size:
2649 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2013 3771 4927 1790
Size: 223 1758 1156 2573
Fragments arranged by size:
2573 1758 1156 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2613 2687 3441
Size: 416 53 83 399 24 1196 74 754
Cuts at: 3441 4399 442
Size: 958 1753
Fragments arranged by size:
1753 1196 958 754 416 399 83 74
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2789
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4370 1239
Size: 3131 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2091 3835 4700 4998 2091
Size: 1744 865 298 2803
Fragments arranged by size:
2803 1744 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2986 3517 3899 4534
Size: 53 83 186 936 1453 531 382 635
Cuts at: 4534 275
Size: 1451
Fragments arranged by size:
1453 1451 936 635 531 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2415 1186
Size: 1229 4481
BseYI C'CCAG_C
Cuts at: 2729 4913 5409 2729
Size: 2184 496 3030
Fragments arranged by size:
3030 2184 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2014 1163
Size: 573 278 4859
Fragments arranged by size:
4859 573 278
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2898 3029 3771 3856 4927 1391
Size: 399 223 885 131 742 85 1071 2174
Fragments arranged by size:
2174 1071 885 742 399 223 131 85
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2100 3065 844
Size: 1256 965 3489
Fragments arranged by size:
3489 1256 965
BsrBI CCG'CTC
Cuts at: 1394 1823 3597 1394
Size: 429 1774 3507
Fragments arranged by size:
3507 1774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2806 4354 4541 1700
Size: 110 996 1548 187 2869
Fragments arranged by size:
2869 1548 996 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 2626 3770 4782 2626
Size: 1144 1012 3554
Fragments arranged by size:
3554 1144 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstYI r'GATC_y
Cuts at: 1972 2357 2604 3040 3407 3792 3809 5261 5347
Size: 385 247 436 367 385 17 1452 86
Cuts at: 5347 5359 5705 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 436 385 385 367 346 247
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4219
Fragments arranged by size:
4219 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 2100 3226 4020 4040 2100
Size: 1126 794 20 3770
Fragments arranged by size:
3770 1126 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5675
DraI TTT'AAA
Cuts at: 2079 3014 2079
Size: 935 4775
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3157 4046 5675
Size: 54 1425 50 275 18 1327 889 1629
Cuts at: 5675 8
Size: 43
Fragments arranged by size:
1629 1425 1327 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3640 1353
Size: 2287 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2456 2958 3773 4370 5157 5425 1239
Size: 393 824 502 815 597 787 268 1524
Fragments arranged by size:
1524 824 815 787 597 502 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2009 2010 2056 2642 2009
Size: 1 46 586 5077
Fragments arranged by size:
5077 586 46 1
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 5710
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 4857 1678
Size: 3179 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 2844 2876 4351
Size: 32 135 32 770 32 470 32 1475
Cuts at: 4351 4383 4425 4457 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1475 770 470 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3668 4027 4186 518
Size: 648 2502 359 159 2042
Fragments arranged by size:
2502 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3126 3803 4951 5196 1606
Size: 1520 677 1148 245 2120
Fragments arranged by size:
2120 1520 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4219
Fragments arranged by size:
4219 939 552
NdeI CA'TA_TG
Cuts at: 387 2248 387
Size: 1861 3849
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NsiI A_TGCA'T
Cuts at: 2320 2320
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2683 3068 4613 1132
Size: 97 1454 385 1545 2229
Fragments arranged by size:
2229 1545 1454 385 97
PasI CC'CwG_GG
Cuts at: 1348 2478 2577 1348
Size: 1130 99 4481
Fragments arranged by size:
4481 1130 99
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5710
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2642 2056
Size: 586 5124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PspOMI G'GGCC_C
Cuts at: 2009 2009
Size: 5710
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2519 2551 2519
Size: 32 5678
PstI C_TGCA'G
Cuts at: 830 3062 830
Size: 2232 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 3058 4193 4874 5065 826
Size: 219 2013 1135 681 191 1471
Fragments arranged by size:
2013 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 2345 3037 1872
Size: 473 692 4545
Fragments arranged by size:
4545 692 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2106 3814 4715 4977 820
Size: 197 926 163 1708 901 262 1553
Fragments arranged by size:
1708 1553 926 901 262 197 163
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 2683 3068 1132
Size: 1551 385 3774
Fragments arranged by size:
3774 1551 385
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 3149 513
Size: 552 939 1145 3074
Fragments arranged by size:
3074 1145 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2146 2682 3737 3754 3907 4092 4537
Size: 1255 566 536 1055 17 153 185 445
Cuts at: 4537 325
Size: 1498
Fragments arranged by size:
1498 1255 1055 566 536 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 2693 1316
Size: 1377 4333
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 2343 3035 1870
Size: 473 692 4545
Fragments arranged by size:
4545 692 473
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2987 3518 276
Size: 53 83 186 936 1453 531 2468
Fragments arranged by size:
2468 1453 936 531 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspXI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI